Abstract：
:Recent studies have demonstrated that direct cell-to-cell interaction is one of the microenvironment factors for transdifferentiation of adult stem cells into cardiomyocytes. We investigated whether transdifferentiation of mesenchymal stem cells (MSCs) into cardiomyocytes was dependent on developmental stages of cocultured cardiomyocytes, and direct cell-to-cell interaction was essential for transdifferentiation. MSCs were isolated from adult rat and cocultured in four different ways: (1) with neonatal cardiomyocytes, (2) with adult cardiomyocytes, (3) with neonatal cardiomyocytes on the cell culture inserts, and (4) with the conditioned medium from neonatal cardiomyocytes. After 5 days of coculture with neonatal cardiomyocytes, 9.40+/-1.15% of 1,1'-dioctadecyl-1-3,3,3',3'-tetramethylindocarbocyanine perchlorate labeled MSCs expressed sarcomeric-alpha-actinin. Immunocytochemistry showed that only these MSCs expressed the cardiac markers and were not observed with other coculture condition as well as conditioned medium. Calcein-AM labeling of cardiomyocytes showed gap junctional communication between 56.1+/-2.0% of MSCs (24 h after labeling, n=5) and neonatal cardiomyocytes. These findings suggest that MSCs are capable of differentiating into cardiomyocytes when directly cocultured with neonatal cardiomyocytes by cell-to-cell interaction, but not with adult cardiomyocytes or conditioned medium.
journal_name
Ann Hematoljournal_title
Annals of hematologyauthors
Yoon J,Shim WJ,Ro YM,Lim DSdoi
10.1007/s00277-005-1068-7subject
Has Abstractpub_date
2005-10-01 00:00:00pages
715-21issue
11eissn
0939-5555issn
1432-0584journal_volume
84pub_type
杂志文章abstract：:An investigation into the effects of irradiation and of the storage time on aging and quality are a relevant issue to ensure the safety and the efficiency of irradiation in the prevention of transfusion-associated graft-versus-host disease (TA-GVHD). In this work, the biochemical properties and alterations presented b...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s00277-013-1944-5
更新日期：2014-05-01 00:00:00
abstract：:Thirty-four patients with acute myeloid leukemia (AML) in complete remission (CR), 30 of them aged over 60, received maintenance therapy scheduling four courses of low-dose cytarabine (LDA) 20 mg/m2/day in two subcutaneous injections for 3 weeks every 6 weeks. Each course was stopped when hematologic toxicity occurred...
journal_title：Annals of hematology
pub_type： 临床试验,杂志文章
doi：10.1007/BF01698132
更新日期：1992-08-01 00:00:00
abstract：:Neutral red is a vital stain known to be accumulated in the lysosomes of neutrophils and monocytes. It is used mainly to identify and detect the activated state of these cells. We have found that the extracellular application of physiological ceramide, i.e., a product of sphingomyelin hydrolysis and a newly defined in...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s002770050015
更新日期：2000-02-01 00:00:00
abstract：:Many studies have confirmed that overexpressed WT1 exists in leukemic cells, especially in AML. However, the immunophenotypic features of this sort of leukemic cells remain to be unclarified. We retrospectively analyzed the immunophenotype of 283 newly diagnosed AML patients with intermediated and poor cytogenetic ris...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s00277-019-03808-6
更新日期：2020-02-01 00:00:00
abstract：:Aplastic anemia is a rare complication of thymoma and is properly documented in only few cases. Here, we report the case of a previously healthy, 65-year-old patient who was found simultaneously to have a spindle-cell thymoma and severe hypoplastic anemia with a mild infiltration of the bone marrow by CD4+ and CD8+ T ...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s002770050437
更新日期：1998-10-01 00:00:00
abstract：:Polycythemias or erythrocytoses in childhood and adolescence are very rare. Systematic data on the clinical presentation and laboratory evaluations as well as on treatment regimens are sparse. The diagnostic program in absolute erythrocytosis includes extensive clinical, hematological, biochemical, and molecular biolo...
journal_title：Annals of hematology
pub_type： 杂志文章,评审
doi：10.1007/s00277-004-0985-1
更新日期：2005-03-01 00:00:00
abstract：:Thirty-nine pregnant women with idiopathic thrombocytopenic purpura (ITP) were studied in order to evaluate the influence of therapies for maternal ITP on fetal passive immune thrombocytopenia (PIT). Neonatal platelet counts were also compared with platelet counts, amount of PAIgG, and presence of circulating antiplat...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/BF01695918
更新日期：1994-01-01 00:00:00
abstract：:Rituximab was recently described also as first-line therapy of chronic graft-versus-host disease (cGvHD). We retrospectively analyzed the efficacy and safety of all patients receiving rituximab for treatment of cGvHD between 2005 and 2016 at the Regensburg University transplant center with a median follow-up after rit...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s00277-019-03768-x
更新日期：2019-10-01 00:00:00
abstract：:Immunoglobulin D (IgD) myeloma is a rare subtype that used to lead to a poor outcome. To investigate the current clinical features, cytogenetic changes and survival of patients with IgD myeloma under novel treatments, we analysed 47 patients with IgD myeloma, 31 men and 16 women, with a median age of 54.5 years. We fo...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s00277-018-3582-4
更新日期：2019-04-01 00:00:00
abstract：:We investigated the efficacy and toxicity of the combination of busulfan, cyclophosphamide, and etoposide (Bu/Cy/VP-16) as a preparative regimen prior to autologous hematopoietic stem cell transplantation (ASCT) in patients with Hodgkin's disease (HD) or non-Hodgkin's lymphoma (NHL). Fifty-three patients with recurren...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s00277-001-0413-8
更新日期：2002-02-01 00:00:00
abstract：:We conducted a phase 2 study with bortezomib, doxorubicin, and dexamethasone (PAD) followed by thalidomide and dexamethasone (TD) in patients with relapsed multiple myeloma (MM). Forty patients were enrolled between November 2005 and October 2007, with follow-up continuing until January 2009. Efficacy could be assesse...
journal_title：Annals of hematology
pub_type： 杂志文章,多中心研究
doi：10.1007/s00277-010-0943-z
更新日期：2010-09-01 00:00:00
abstract：:Children with sickle cell disease (SCD) benefit from newborn screening, because life-threatening complications can be prevented by pre-symptomatic diagnosis. In Germany, the immigration of people from endemic countries is steadily growing. Comprehensive data about the epidemiology and prevalence of SCD in Germany are ...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s00277-015-2573-y
更新日期：2016-02-01 00:00:00
abstract：:Hematopoietic recovery after high-dose chemotherapy (HDC) in the treatment of hematological diseases may be slow and/or incomplete. This is generally attributed to progressive hematopoietic stem cell failure, although defective hematopoiesis may be in part due to poor stromal function. Chemotherapy is known to damage ...
journal_title：Annals of hematology
pub_type： 临床试验,杂志文章
doi：10.1007/s00277-009-0896-2
更新日期：2010-07-01 00:00:00
abstract：:Solitary plasmacytoma of bone (SPB) is a rare tumor that represents a minority of patients with plasma cell localized malignancy characterized by a single osteolytic bone lesion. The molecular mechanism underlying the genesis of SPB has remained enigmatic. Signal transducers and activators of transcription-3 (STAT3) i...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s00277-013-1897-8
更新日期：2014-03-01 00:00:00
abstract：:Platelet concentrates were pretreated with a stable synthetic prostacyclin analogue (Iloprost) at two different concentrations before the second centrifugation step (pelleting step) of preparation. This resulted in loss of platelet sensitivity to aggregating agents. To mimic the situation after transfusion and to asse...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/BF01695474
更新日期：1992-06-01 00:00:00
abstract：:Transplantation of peripheral blood stem cells (PBSC), positively and/or negatively selected immediately after harvest, has become a widely applied therapeutic option in hematological or oncological patients. The following case of peripheral blood stem cell transplantation represents the first case of successful trans...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s002770000243
更新日期：2001-02-01 00:00:00
abstract：:A case of an 82-year-old man who suffered an acute ST-elevation myocardial infarction while receiving treatment with intravenous immunoglobulin (IVIg) for thrombocytopenia is discussed. A total of 29 other cases of thromboembolism related to IVIg therapy have been reported, and the incidence seems to be especially hig...
journal_title：Annals of hematology
pub_type： 杂志文章,评审
doi：10.1007/s00277-004-0895-2
更新日期：2004-10-01 00:00:00
abstract：:Elderly and infirm patients with acute myeloid leukemia (AML) with either induction refractory or relapse disease may benefit from treatment with azacitidine. We retrospectively reviewed the data from five tertiary centers in Israel, treated between 2009 and 2015. Thirty-four patients (median age 74 years) were identi...
journal_title：Annals of hematology
pub_type： 杂志文章,多中心研究
doi：10.1007/s00277-016-2914-5
更新日期：2017-04-01 00:00:00
abstract：:Only about one third of all patients with acute myeloid leukemia (AML) will be cured by common chemotherapy regimens. Susceptibility towards chemotherapy either of the leukemic bulk or the leukemic stem cell is considered the major determining parameter for long-term outcome. The purpose of the present study was to in...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s00277-007-0361-z
更新日期：2008-01-01 00:00:00
abstract：:Despite high-dose chemotherapy and autografting, the outcome for patients with primary refractory Hodgkin's disease (HD) or multiple relapses remains unsatisfactory. Six pediatric patients (median age: 16 years, range: 11-19) received reduced intensity conditioning and allogeneic peripheral blood stem cell transplanta...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s00277-003-0814-y
更新日期：2004-04-01 00:00:00
abstract：:Uptake and biochemical and biological effects of antisense oligodeoxynucleotides (ODN) specific for c-abl and bcr genes were studied in normal immature myeloid cells. CD34-positive cells were purified by positive and negative selection and cultured in liquid culture for 7 days. These cells were then incubated with ODN...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/BF00641311
更新日期：1996-02-01 00:00:00
abstract：:Although lenalidomide plus dexamethasone (RD) is a therapeutic option for relapsed/refractory multiple myeloma (RRMM), limited real-world clinical data exist. The purpose of this study was to estimate efficacy and safety of RD in RRMM patients of the clinical practice. Data from patients at 25 university hospitals in ...
journal_title：Annals of hematology
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1007/s00277-019-03904-7
更新日期：2020-02-01 00:00:00
abstract：:Angioimmunoblastic lymphadenopathy with dysproteinemia (AILD) is an atypical lympho-proliferative disorder with borderline features that often constitute a diagnostic challenge for the hematopathologist and a therapeutic dilemma for the treating clinician. Morphologically, the involved lymph nodes in this disorder are...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s002770050244
更新日期：1996-12-01 00:00:00
abstract：:A prospective randomized phase III study was performed to evaluate whether intensified cytarabine would induce a higher response rate and longer event-free interval as compared to low-dose cytarabine in chronic myeloid leukemia (CML). One hundred and eighteen patients with CML in early chronic phase entered the study....
journal_title：Annals of hematology
pub_type： 杂志文章,随机对照试验
doi：10.1007/s00277-006-0186-1
更新日期：2007-02-01 00:00:00
abstract：:Primary non-Hodgkin's lymphoma of bone (PLB) is a rare entity. We present three new cases and a review of the literature. If PLB is suspected radiologically, the diagnosis must be confirmed by open biopsy. Histopathologically, PLB usually represents diffuse large B-cell lymphoma or lymphoma of the follicular center ty...
journal_title：Annals of hematology
pub_type： 杂志文章,评审
doi：10.1007/s00277-003-0674-5
更新日期：2003-07-01 00:00:00
abstract：:Eight patients who were carriers of beta-thalassemia induced by the cd121 (G-->T) mutation are described in four nonrelated Dutch families. This mutant, which is considered rare and inherited in a dominant manner, is expressed in a different way among each of the four families and even among carriers of the same famil...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s002770050453
更新日期：1998-12-01 00:00:00
abstract：:The purpose of this cross-sectional prospective study was to determine the prevalence of anemia among elderly hospitalized patients in Germany and to investigate its association with multidimensional loss of function (MLF). One hundred participants aged 70 years or older from two distinct wards (50 each from an emerge...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s00277-014-2110-4
更新日期：2014-10-01 00:00:00
abstract：:The laboratory diagnosis of hereditary spherocytosis (HS) is based on several screening and confirmatory tests; our algorithm includes clinical features, red blood cell morphology analysis and cryohaemolysis test, and, in case of positive screening, sodium dodecyl sulphate polyacrylamide gel electrophoresis as a diagn...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s00277-014-2127-8
更新日期：2014-11-01 00:00:00
abstract：:Idarubicin belongs to a group of anthracyclines in which the methoxyl group in position 4 of the D ring in the aglycone moiety is replaced by a hydrogen atom. Lipophilicity is increased compared with other anthracyclines; as a result, idarubicin is the first anthracycline that can be administered orally while at the s...
journal_title：Annals of hematology
pub_type： 杂志文章,评审
doi：10.1007/BF01737687
更新日期：1993-01-01 00:00:00
abstract：:Reactive oxygen intermediates (ROI) generated in the respiratory burst reaction are crucial for the killing of bacteria and fungi in phagocytes. The key enzyme for the respiratory burst reaction is the NADPH oxidase. Reactive oxygen intermediates have additionally been proposed to be of general importance for the expr...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s00277-003-0837-4
更新日期：2004-04-01 00:00:00
