Abstract:
:Neutral red is a vital stain known to be accumulated in the lysosomes of neutrophils and monocytes. It is used mainly to identify and detect the activated state of these cells. We have found that the extracellular application of physiological ceramide, i.e., a product of sphingomyelin hydrolysis and a newly defined intracellular second-messenger substance, increased the uptake of neutral red in a dose-dependent manner in human neutrophils, monocytes, and lymphocytes, as demonstrated by flow cytometry. Staurosporine was able to totally block this phenomenon, suggesting the involvement of protein kinase C in the process. These results indicate that the flow-cytometric analysis of ceramide-induced uptake of neutral red can be a new method for the evaluation of lysosome-related activation processes in both phagocytes and lymphocytes.
journal_name
Ann Hematoljournal_title
Annals of hematologyauthors
Sipka S,Antal-Szalmás P,Szöllösi I,Csipö I,Lakos G,Szegedi Gdoi
10.1007/s002770050015subject
Has Abstractpub_date
2000-02-01 00:00:00pages
83-5issue
2eissn
0939-5555issn
1432-0584journal_volume
79pub_type
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