Abstract：
:Antithymocyte globulin (ATG) is the treatment of choice for those aplastic anemia patients who are not suitable for bone marrow transplantation (BMT). ATG is also used for the treatment of rejections in organ transplantation and as a conditioning regimen in BMT. Despite the proven efficacy of ATG in these areas, its mechanism of action is not known. Profound T-cell lymphopenia observed in vivo with ATG treatment is supposed to contribute to its therapeutic effect. We have previously shown that apoptosis is one of the mechanisms responsible for ATG-induced lymphopenia. Our next objective was to investigate the effect of ATG on modulation of Fas and TNF pathways, the two main pathways of T-cell apoptosis. Maximum surface expression of Fas on T cells was observed after 24 h at an ATG dose of 100 microg/ml; at this dose 88% of cells expressed Fas as compared to 26% of untreated cells. Surface expression of FasL was found to peak after 24 h at an ATG dose of 1000 microg/ml when 34% of cells were positive for FasL as compared to 1.5% of untreated T cells. Tumor necrosis factor (TNF)-alpha production was found to be maximum after 6 h at 1000 microg/ml dose (20%) as measured by intracellular cytokine staining of T cells. TNF-alpha production was also measured by enzyme-linked immunosorbent assay (ELISA) in the supernatant of lymphocytes treated with ATG for 6 h. A dose-dependent increase in TNF-alpha production was found in these supernatants with a plateau being achieved at an ATG dose of 1000 micro g/ml. We conclude that ATG-induced apoptosis in T cells involves both Fas and TNF pathways and TNF-alpha is produced much earlier than Fas and FasL expression.
journal_name
Ann Hematoljournal_title
Annals of hematologyauthors
Dubey S,Nityanand Sdoi
10.1007/s00277-003-0645-xsubject
Has Abstractpub_date
2003-08-01 00:00:00pages
496-499issue
8eissn
0939-5555issn
1432-0584pii
10.1007/s00277-003-0645-xjournal_volume
82pub_type
杂志文章abstract：:Autologous stem cell transplant (ASCT) is standard consolidation therapy in management of multiple myeloma (MM) patients. We reviewed records of all consecutive MM patients who underwent ASCT with high-dose melphalan at our center from year 2002 to 2016. A total of 141 ASCT were conducted (90 males and 51 females) wit...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s00277-018-3370-1
更新日期：2018-10-01 00:00:00
abstract：:Toxoplasmosis is one of the most common parasitic infections in humans, but in most cases it does not cause serious illness; this protozoan can nevertheless cause devastating disease in immunocompromised hosts such as HIV-positive individuals. Only rarely is toxoplasmosis documented in hematological patients, and amon...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s00277-004-0898-z
更新日期：2004-09-01 00:00:00
abstract：:Abnormal blood coagulation often occurs in critically ill patients, which seriously affects their prognosis. This retrospective study investigated the implications of changes in blood coagulation in patients with coronavirus disease 2019 (COVID-19). Records were reviewed for patients admitted with COVID-19 between Feb...
journal_title：Annals of hematology
pub_type： 临床试验,杂志文章
doi：10.1007/s00277-020-04305-x
更新日期：2021-01-01 00:00:00
abstract：:Solitary plasmacytoma of bone (SPB) is a rare tumor that represents a minority of patients with plasma cell localized malignancy characterized by a single osteolytic bone lesion. The molecular mechanism underlying the genesis of SPB has remained enigmatic. Signal transducers and activators of transcription-3 (STAT3) i...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s00277-013-1897-8
更新日期：2014-03-01 00:00:00
abstract：:Hematopoietic chimerism can be used as a tool for patient management after allogeneic hematopoietic stem cell transplantation (HSCT). An increase in the proportion of recipient cells after transplantation is strongly associated with relapse in chronic myeloid leukemia. However, in acute myeloid leukemia (AML) the sign...
journal_title：Annals of hematology
pub_type： 临床试验,杂志文章
doi：10.1007/s00277-013-1858-2
更新日期：2014-02-01 00:00:00
abstract：:Telomere shortening represents an established mechanism connecting aging and cancer development. We sequentially analyzed telomere length (TL) of 49 acute myeloid leukemia (AML) patients at diagnosis (n = 24), once they achieved complete cytological remission (CCR) and/or during refractory disease or relapse and after...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s00277-017-3049-z
更新日期：2017-09-01 00:00:00
abstract：:Lymphoma-associated hemophagocytic lymphohistiocytosis (HLH) is a rare but fatal disease. Differences between B cell and T cell lymphoma-associated HLH remain unclear, specifically clinical characteristics and survival. We retrospectively analyzed 30 lymphoma-associated HLH patients from July 2004 to October 2012. Pat...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s00277-013-1784-3
更新日期：2013-11-01 00:00:00
abstract：:We investigated the efficacy of an antithymocyte globulin/cyclophosphamide preparative regimen prior to allogeneic stem cell transplantation from HLA-identical siblings in patients with severe aplastic anemia. Since 1990, 21 patients, 6 males and 15 females, with a median age of 25 years (range: 7-43) have been enroll...
journal_title：Annals of hematology
pub_type： 临床试验,杂志文章
doi：10.1007/s00277-002-0566-0
更新日期：2002-11-01 00:00:00
abstract：:The clinical targets for which new generations of antithrombotics have been or are currently under clinical development are those associated with a high risk for thromboembolism, (a) patients undergoing general, orthopedic, major abdominal, and cancer surgery, to prevent venous thromboembolism; (b) patients with deep-...
journal_title：Annals of hematology
pub_type： 杂志文章,评审
doi：10.1007/s002770050158
更新日期：1996-04-01 00:00:00
abstract：:Several guidelines and recommendations on the management of chronic myeloid leukemia (CML) have been prepared by several scientific societies. The European LeukemiaNet (ELN) appointed a panel of experts who submitted their recommendations to peer-reviewed scientific journals in 2006, 2009, and 2013. Here, we make a cr...
journal_title：Annals of hematology
pub_type： 杂志文章,评审
doi：10.1007/s00277-015-2322-2
更新日期：2015-04-01 00:00:00
abstract：BACKGROUND:Low folate intake and changes in folate metabolism due to polymorphisms in the methylentetrahydrofolate reductase (MTHFR) gene have been associated with myelomagenesis. However, controversial data have been published regarding a protective role of variant alleles of MTHFR on MM. PATIENTS AND METHODS:To inve...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s00277-006-0097-1
更新日期：2006-07-01 00:00:00
abstract：:Idarubicin belongs to a group of anthracyclines in which the methoxyl group in position 4 of the D ring in the aglycone moiety is replaced by a hydrogen atom. Lipophilicity is increased compared with other anthracyclines; as a result, idarubicin is the first anthracycline that can be administered orally while at the s...
journal_title：Annals of hematology
pub_type： 杂志文章,评审
doi：10.1007/BF01737687
更新日期：1993-01-01 00:00:00
abstract：:The combination of sickle cell disease crisis and thrombotic thrombocytopenic purpura has been described only a few times. Here we present the case of a patient with a hemolytic crisis due to sickle cell disease complicated by thrombotic thrombocytopenic purpura. We also review the cases previously reported and compar...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s002770050558
更新日期：1999-12-01 00:00:00
abstract：:Treatment of relapse and primary progression in aggressive lymphoma remains unsatisfactory; outcome is still poor. Better treatment strategies are much needed for this patient population. The R1 study is a prospective multi-center phase I/II study evaluating a dose finding approach with a triple transplant regimen in ...
journal_title：Annals of hematology
pub_type： 杂志文章,多中心研究
doi：10.1007/s00277-016-2671-5
更新日期：2016-06-01 00:00:00
abstract：:Transplantation of autologous hematopoietic stem cells is a well established therapeutic procedure. Despite advances in efficacy of the stem cell mobilization and apheresis process until now a predictive factor for the expected stem cell yield before initiation of mobilization therapy could not be identified. The main...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s00277-005-0074-0
更新日期：2006-06-01 00:00:00
abstract：:In this report we show that serum has differentiation-inducing effects on primitive hematopoietic progenitor cells with the CD34++CD38- immunophenotype. Using the pre-colony forming unit (pre-CFU) assay as a model for early myelopoiesis, we compared the effects of serum-containing and serum-free media and evaluated di...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s002770000227
更新日期：2001-01-01 00:00:00
abstract：:Subcutaneous panniculitis-like T-cell lymphoma (SPTCL) is an uncommon type of cutaneous lymphoma with an aggressive natural history. It generally carries a poor prognosis despite standard anthracycline-based chemotherapy. The optimum therapy is unknown. We report the case of a 66-year-old man with CD4/CD8 double negat...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s00277-003-0638-9
更新日期：2003-04-01 00:00:00
abstract：:Ruxolitinib is a promising option for treating steroid-refractory acute graft-versus-host disease (SR-aGVHD) after allogeneic hematopoietic stem cell transplantation (allo-HSCT). In this study, we describe ruxolitinib treatment for SR-aGVHD in HSCT patients with Epstein-Barr virus-associated hemophagocytic lymphohisti...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s00277-019-03864-y
更新日期：2020-02-01 00:00:00
abstract：:Secondary hemophagocytic lymphohistiocytosis (HLH) is a rare but fatal condition with various underlying disorders in adult patients and is diagnosed based on the HLH-2004 criteria, which were established based on experience in pediatric patients. However, few studies have prospectively evaluated the treatment outcome...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s00277-020-04083-6
更新日期：2020-09-01 00:00:00
abstract：:We have studied some of the factors involved in the cytotoxic actions of the anticancer anthracycline antibiotics doxorubicin (DOX) and idarubicin (IDA) towards human B-cell lymphoma cells in vitro. IDA was found to accumulate within cells to a greater degree than the related drug DOX for both short (1 h) and long-ter...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/BF01757348
更新日期：1994-01-01 00:00:00
abstract：:In hematopoietic system development, PU.1 and GATA-1 as lineage-specific transcription factors (TF) are expressed in common myeloid progenitors. The cross antagonism between them ascertains gene expression programs of monocytic and erythroid cells, respectively. This concept in transdifferentiation approaches has not ...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s00277-015-2583-9
更新日期：2016-03-01 00:00:00
abstract：:The optimal treatment strategy for elderly patients with natural killer/T-cell lymphoma (NKTCL) remains to be established. A total of 63 elderly patients with newly diagnosed NKTCL were retrospectively reviewed. Among the patients with stage I-II disease, 58.3 % received radiotherapy (RT) ± chemotherapy, and 41.7 % re...
journal_title：Annals of hematology
pub_type： 临床试验,杂志文章
doi：10.1007/s00277-015-2395-y
更新日期：2015-09-01 00:00:00
abstract：:Autologous stem cell transplant (ASCT) is an effective treatment for non-Hodgkin lymphoma (NHL). However, recent supply issues and toxicity of carmustine have necessitated a new conditioning regimen. We conducted a multicenter, phase II study of BEB (busulfan, etoposide, and bendamustine) conditioning regimen for ASCT...
journal_title：Annals of hematology
pub_type： 杂志文章,多中心研究
doi：10.1007/s00277-020-03942-6
更新日期：2020-04-01 00:00:00
abstract：:Extreme thrombocytosis (ExT) has been associated with an increased bleeding risk in myeloproliferative neoplasm (MPN) patients and is included in the high risk category in treatment guidelines. Treatment of patients with ExT has not been studied in prospective trials. To study physicians' approaches to ExT, we distrib...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s00277-016-2826-4
更新日期：2017-01-01 00:00:00
abstract：:Rituximab was recently described also as first-line therapy of chronic graft-versus-host disease (cGvHD). We retrospectively analyzed the efficacy and safety of all patients receiving rituximab for treatment of cGvHD between 2005 and 2016 at the Regensburg University transplant center with a median follow-up after rit...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s00277-019-03768-x
更新日期：2019-10-01 00:00:00
abstract：:Aberrant promoter methylation of RASSF6 and RASSF10 occurs at a high frequency in acute lymphoblastic leukemia (ALL). Because of the complexity of the current minimal residual disease (MRD) detecting-methods, the DNA methylation status of the RASSF6 and RASSF10 genes could potentially become biomarkers for the assessm...
journal_title：Annals of hematology
pub_type： 临床试验,杂志文章
doi：10.1007/s00277-019-03775-y
更新日期：2019-12-01 00:00:00
abstract：:The matricellular protein SPARC (secreted protein acidic and rich in cysteine)/osteonectin was determined in patients with multiple myeloma and related disease to assess the hypothesized role of SPARC as a possible marker of tumor burden and disease progression. Soluble SPARC was measured by competitive enzyme-linked ...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s00277-004-0990-4
更新日期：2005-05-01 00:00:00
abstract：:The aim of this study was to analyze the ability of an alloantibody from a patient with severe von Willebrand disease (vWD) to interfere with the vWF domain for FVIII, to inhibit factor VIII (FVIII), and to compare it with a rabbit polyclonal antibody. The vWF domain for binding to FVIII was assayed by a method previo...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s002770050323
更新日期：1997-09-01 00:00:00
abstract：:Hemophagocytic lymphohistiocytosis (HLH) is a rare multisystem disorder characterized by proliferation and diffuse infiltration multiple organs with histiocytes, including the central nervous system (CNS). Neurological manifestations of HLH have been recognized in different studies with children, but they remain relat...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s00277-017-3035-5
更新日期：2017-08-01 00:00:00
abstract：:HbE/Beta thalassemia (HbE/β-thalassemia) is one of the common genetic disorders in South East Asia. It is heterogeneous in its clinical presentation and molecular defects. There are genetic modifiers which have been reported to influence the disease severity of this disorder. The aim of this study was to determine the...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s00277-020-03927-5
更新日期：2020-04-01 00:00:00
