Abstract:
:In this report we show that serum has differentiation-inducing effects on primitive hematopoietic progenitor cells with the CD34++CD38- immunophenotype. Using the pre-colony forming unit (pre-CFU) assay as a model for early myelopoiesis, we compared the effects of serum-containing and serum-free media and evaluated different cytokine cocktails [interleukin (IL)-1, IL-3, IL-6, kit ligand with and without the Flt3/Flk2 ligand (FL)]. In this assay, pre-CFUs are defined as cells unable to form colonies when plated directly in semi-solid assays, but which can differentiate into CFUs when cultured in liquid medium containing early-acting cytokines. In one of the investigated serum-free media, the average myeloid expansion in liquid medium reached up to more than 50% of that obtained in serum-containing medium. In addition, our experiments revealed differences in the clonogenic output between cells cultured in serum-free medium and those cultured in serum-containing medium, demonstrating that serum has a monocyte differentiation-inducing effect on primitive hematopoietic progenitors. Also in serum-free medium, higher proportions of erythroid progenitors were generated. These differentiation-inducing effects of serum further emphasize the need for serum-free culture protocols for hematopoietic graft engineering. Addition of FL to the culture media ameliorated cellular expansion and resulted in a decrease in the proportion of erythroid and granulocyte progenitors and an increase in the proportion of monocyte progenitors. In conclusion, this study shows that good serum-free conditions are available for differentiation assays with primitive hematopoietic progenitors and demonstrates that serum and FL have biasing effects on the initial phase of hematopoietic differentiation, favoring the monocyte lineage.
journal_name
Ann Hematoljournal_title
Annals of hematologyauthors
Willems R,Henckaerts E,Lenjou M,Nijs G,Rodrigus I,Moulijn AC,Slegers H,Berneman ZN,Van Bockstaele DRdoi
10.1007/s002770000227subject
Has Abstractpub_date
2001-01-01 00:00:00pages
17-25issue
1eissn
0939-5555issn
1432-0584journal_volume
80pub_type
杂志文章abstract::t(8;16)(p11.2;p13.3)/KAT6A-CREBBP is a rare recurrent cytogenetic abnormality associated with acute myeloid leukemia (AML). We report 15 cases with t(8;16)(p11.2;p13.3). All patients were adult and had AML: 13 women and 2 men, with a median age of 50 years. Ten patients had a history of malignancy and received cytotox...
journal_title:Annals of hematology
pub_type: 临床试验,杂志文章
doi:10.1007/s00277-019-03637-7
更新日期:2019-05-01 00:00:00
abstract::Chronic graft-versus-host disease (cGVHD) is an important complication after allogeneic hematopoietic stem cell transplantation (HSCT). To define the roles of T-cells and B-cells in cGVHD, a murine minor histocompatibility complex-mismatched HSCT model was used. Depletion of donor splenocyte CD4(+) T-cells and B220(+)...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-015-2394-z
更新日期:2015-09-01 00:00:00
abstract::Stem cell factor (SCF), also termed mast cell growth factor or c-kit ligand, plays a central role in the regulation of hematopoiesis and maintenance of viability of hematopoietic cells. We used a new murine monoclonal antibody (MAb) specific for canine SCF to further dissect the role of SCF in vitro and in vivo. This ...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s002770050168
更新日期:1996-04-01 00:00:00
abstract::Angioimmunoblastic lymphadenopathy with dysproteinemia (AILD) is an atypical lympho-proliferative disorder with borderline features that often constitute a diagnostic challenge for the hematopathologist and a therapeutic dilemma for the treating clinician. Morphologically, the involved lymph nodes in this disorder are...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s002770050244
更新日期:1996-12-01 00:00:00
abstract::Increasing the number of megakaryocytic cells in stem cell transplants by ex vivo expansion culture may provide an approach to accelerate platelet engraftment after high-dose chemotherapy. However, it is unknown if a relationship exists between the expansion potential of progenitor cells and the time to platelet engra...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-002-0443-x
更新日期:2002-04-01 00:00:00
abstract::A 64-year-old woman with adult T cell leukemia (ATL) was admitted to our hospital with severe hypercalcemia. The serum calcium level was elevated to 14.9 mg/dl. Biochemical parameters for bone formation including serum osteocalcin (bone Gla protein, BGP) and alkaline phosphatase (ALP) were normal. The serum levels of ...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s002770100305
更新日期:2001-07-01 00:00:00
abstract::We present a patient with chronic myelomonocytic leukemia who showed disseminated papules and nodules. Arguments in favor of leukemia cutis are the clinical appearance, the cyclic pattern with which the lesions appeared and disappeared, and the histologic features. The lesions reproducibly responded to treatment with ...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s002770000156
更新日期:2000-07-01 00:00:00
abstract::A 57-year-old man with acute myeloid leukemia (AML) French-American-British (FAB) 4 developed disseminated invasive cerebral and pulmonary aspergillosis during postinduction aplasia. According to international consensus, infection was categorized as probable (two host factors: deep neutropenia for >10 days and refract...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-003-0792-0
更新日期:2004-06-01 00:00:00
abstract::The appropriate management of patients with moderate aplastic anemia (mAA) remains to be unclear and controversial. A cohort of 118 patients with mAA received a novel immunosuppressive strategy of cyclosporine alternately combined with levamisole (CSA and LMS regimen), which included 42 newly diagnosed and 76 chronic ...
journal_title:Annals of hematology
pub_type: 临床试验,杂志文章
doi:10.1007/s00277-013-1764-7
更新日期:2013-09-01 00:00:00
abstract::This study aimed to determine whether dose-dense therapy improves 3-year survival over the standard therapy for untreated aggressive lymphoma. One hundred and fifteen patients with untreated aggressive lymphoma were stratified by center, age, and international prognostic index and randomized to one of two treatment ar...
journal_title:Annals of hematology
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00277-009-0811-x
更新日期:2010-03-01 00:00:00
abstract::Somatic mutations in the SF3B1 gene, a gene encoding the splicing factor 3B subunit 1, were recently reported in myelodysplastic syndromes (MDS), particularly in the presence of ring sideroblasts (RS). The authors investigated the prevalence and clinical significance of SF3B1 mutations in Korean patients with myeloid ...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-013-1915-x
更新日期:2014-04-01 00:00:00
abstract::The presenting features of 356 previously untreated multiple myeloma (MM) patients grouped according to age were analyzed in order (a) to elucidate the possible differences in initial clinical and laboratory features between patients younger than 50 years and the older ones and (b) to statistically assess the prognost...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s002770050365
更新日期:1998-02-01 00:00:00
abstract::Hematopoietic recovery after high-dose chemotherapy (HDC) in the treatment of hematological diseases may be slow and/or incomplete. This is generally attributed to progressive hematopoietic stem cell failure, although defective hematopoiesis may be in part due to poor stromal function. Chemotherapy is known to damage ...
journal_title:Annals of hematology
pub_type: 临床试验,杂志文章
doi:10.1007/s00277-009-0896-2
更新日期:2010-07-01 00:00:00
abstract::Relapse remains one of the major obstacles in Philadelphia chromosome-positive acute lymphoblastic leukemia (Ph+ALL) even after allogeneic hematopoietic stem cell transplantation. The persistence of leukemia-propagating cells (LPCs) may lead to the recurrence of Ph+ALL. Using a xenograft assay, LPCs enrichment in the ...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-018-3253-5
更新日期:2018-05-01 00:00:00
abstract::Recent studies in iron-depleted women have challenged the current approach of treating iron-deficiency anemia (IDA) with oral iron in divided daily doses. Alternate day dosing leads to more fractional absorption of iron. In this randomized controlled trial, we looked at the efficacy and safety of alternate-day (AD) ve...
journal_title:Annals of hematology
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00277-019-03871-z
更新日期:2020-01-01 00:00:00
abstract::In 1989, a prospective randomized multicenter study was initiated in order to determine the safety and efficacy of oral clodronate in myeloma patients. The primary objective of this long-term trial is to evaluate whether supportive clodronate is able to prevent or retard the progression of bone disease and reduce the ...
journal_title:Annals of hematology
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1007/BF01697625
更新日期:1993-03-01 00:00:00
abstract::Since December 2019, a novel coronavirus has spread throughout China and across the world, causing a continuous increase in confirmed cases within a short period of time. Some studies reported cases of thrombocytopenia, but hardly any studies mentioned how the virus causes thrombocytopenia. We propose several mechanis...
journal_title:Annals of hematology
pub_type: 杂志文章,评审
doi:10.1007/s00277-020-04019-0
更新日期:2020-06-01 00:00:00
abstract::Indolent primary cutaneous B cell lymphomas (PCBCL) generally have a good prognosis, but they often relapse leading in some cases to extracutaneous disease and therefore, to poor survival. We developed a prognostic model to improve the therapeutic approach to these lymphomas. Two hundred and seventeen patients with di...
journal_title:Annals of hematology
pub_type: 杂志文章,多中心研究
doi:10.1007/s00277-010-1083-1
更新日期:2011-04-01 00:00:00
abstract::Imatinib is a new promising therapeutic option for chronic myeloid leukemia (CML) with efficacy even in the blast phase of the disease. However, most patients treated with imatinib in the blast phase develop progressive disease rapidly. Thus, treatment with imatinib has to be followed by other treatment strategies. Th...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-003-0643-z
更新日期:2003-05-01 00:00:00
abstract::Arsenic trioxide (As2O3) is a highly effective agent in the treatment of acute promyelocytic leukemia (APL), whereas other hematopoietic tumors are less responsive to this agent and mechanisms underlying As2O3,-resistance are poorly understood. To better understand the complex network of GSH-related pathways in As2O3 ...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-006-0139-8
更新日期:2006-10-01 00:00:00
abstract::The response to intravenous immunoglobulin treatment (IVIG) is thought, in part, to be due to blockade of Fc receptor for IgG in the mononuclear phagocyte system (MPS). We have studied this by measuring splenic clearance of heat-damaged and IgG antibody-coated red cells in immune thrombocytopenic patients receiving IV...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/BF01698483
更新日期:1994-08-01 00:00:00
abstract::We screened for increased osmotic fragility of erythrocytes in 1464 healthy German blood donors. The osmotic fragility was determined by an acidified glycerol lysis test (AGLT) using glycerol-sodium phosphate-buffered NaCl solution. Since the original test described by Zanella et al. [23] showed only low specificity f...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/BF01715351
更新日期:1992-02-01 00:00:00
abstract::Immune thrombocytopenia (ITP) is characterised by a transient or persistent decrease in platelets accompanied by an increased risk of bleeding, which can have a significant negative impact on patients' health-related quality of life. The condition has long been associated with an increased rate of immune-mediated plat...
journal_title:Annals of hematology
pub_type: 杂志文章,评审
doi:10.1007/s00277-010-0953-x
更新日期:2010-07-01 00:00:00
abstract::In this paper we report a rare association of a splenic marginal zone B-cell lymphoma with villous lymphocytes and a T-cell large granular lymphocytic leukemia coexpressing CD4 and CD8 as well as CD56 and CD57 natural killer-associated markers in an asymptomatic patient investigated because of an occasional finding of...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s002770100369
更新日期:2001-11-01 00:00:00
abstract::The combination of sickle cell disease crisis and thrombotic thrombocytopenic purpura has been described only a few times. Here we present the case of a patient with a hemolytic crisis due to sickle cell disease complicated by thrombotic thrombocytopenic purpura. We also review the cases previously reported and compar...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s002770050558
更新日期:1999-12-01 00:00:00
abstract::To elucidate the role of hematopoietic growth factors (HGFs) and other cytokines in the autocrine or paracrine regulation of inducible hematopoiesis we studied cytokine gene expression in the bone marrow (BM) of patients after myelosuppressive treatment. Furthermore, we studied the cytokine gene expression profile in ...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s002770050308
更新日期:1997-07-01 00:00:00
abstract::To investigate the problem of allogeneic bone marrow transplantation (allo-BMT) for advanced stage patients, we retrospectively analyzed 24 consecutive patients who underwent allo-BMT in the non-remission stage. Twenty-four patients (19 males and 5 females) with acute leukemia, chronic myelogenous leukemia, and malign...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-002-0506-z
更新日期:2002-10-01 00:00:00
abstract::The prognostic value of peripheral blasts (PB) is not well-studied in patients with myelodysplastic syndromes (MDS). We evaluated the impact of PB on overall survival (OS) and transformation to acute myeloid leukemia (AML) in a large cohort. The MDS database at the Moffitt Cancer Center was retrospectively reviewed to...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-017-3186-4
更新日期:2018-02-01 00:00:00
abstract::Most cases of erythrocytosis occur secondary to chronic tissue hypoxia or as a clonal disease such as polycythemia vera with somatic mutations in the Janus kinase 2 (JAK2) gene. Rarely, erythrocytosis is caused by hereditary gene mutations. This study investigated hereditary gene mutations in 38 unrelated Korean patie...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-014-2006-3
更新日期:2014-06-01 00:00:00
abstract::Wilms' tumor gene 1 (WT1) is gaining increasing attention as a therapeutic target molecule due to its common expression in acute leukemias and its involvement in cell proliferation. Here, we reported on WT1 messenger RNA expression levels at diagnosis in a series of 238 adult acute lymphoblastic leukemia (ALL) samples...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-009-0746-2
更新日期:2009-12-01 00:00:00