Abstract:
:Reactive oxygen intermediates (ROI) generated in the respiratory burst reaction are crucial for the killing of bacteria and fungi in phagocytes. The key enzyme for the respiratory burst reaction is the NADPH oxidase. Reactive oxygen intermediates have additionally been proposed to be of general importance for the expression of FAS and soluble FAS ligand (sFASL) and the subsequent induction of apoptosis. This conclusion has been drawn from the observation that neutrophils with an inborn lack of the NADPH oxidase as well as cell lines and monocytes with artificially blocked NADPH oxidase exhibit impaired apoptosis. Being one of the few centers caring for patients with chronic granulomatous disease (CGD) who exhibit an inborn lack of NADPH oxidase, we had the unique opportunity to determine the role of the NADPH oxidase for apoptosis in monocytes with otherwise unmanipulated cells of these patients (CGD monocytes). We compared the expression of FAS on monocytes and the concentration of sFASL in the supernatant between CGD monocytes and healthy donors undergoing spontaneous apoptosis. Neither the expression of FAS nor the concentration of sFASL was decreased in CGD monocytes. We further compared spontaneous apoptosis and apoptosis occurring after the phagocytosis of Staphylococcus aureus in CGD monocytes to monocytes of healthy controls. In these experiments we could not determine any significant impairment of apoptosis in CGD monocytes. Our data indicate for the first time that in an unmanipulated human model a functional NADPH oxidase is not crucial for the apoptosis of monocytes and disprove a general role of ROI for the induction of apoptosis in phagocytes.
journal_name
Ann Hematoljournal_title
Annals of hematologyauthors
v Bernuth H,Kulka C,Roesler J,Gahr M,Rösen-Wolff Adoi
10.1007/s00277-003-0837-4subject
Has Abstractpub_date
2004-04-01 00:00:00pages
206-11issue
4eissn
0939-5555issn
1432-0584journal_volume
83pub_type
杂志文章abstract::Approximately, 4-11 % of the patients with idiopathic venous thrombosis (VT) show protein C (PC) deficiency. The molecular pathology of PC deficiency was analyzed in 102 patients; 98 healthy controls were also studied to assess the association of various polymorphisms with reduced PC levels. PROC gene mutations were d...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-012-1483-5
更新日期:2012-09-01 00:00:00
abstract::In some instances, because of the lack of mass formation and the absence of prominent lymph node enlargement, diagnosis of lymphoma-associated hemophagocytic syndrome (LAHS) is difficult, which results in the development of progressive disease with unfavorable prognosis. Therefore, in the diagnosis of secondary hemoph...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-011-1239-7
更新日期:2012-01-01 00:00:00
abstract::Patients with non-Hodgkin's lymphoma (NHL) receiving rituximab-containing chemotherapy are at risk of developing respiratory complications, but comprehensive information on these complications and their impact on survival is lacking. We performed a retrospective cohort analysis on 123 NHL patients who received rituxim...
journal_title:Annals of hematology
pub_type: 临床试验,杂志文章
doi:10.1007/s00277-018-3448-9
更新日期:2018-12-01 00:00:00
abstract::The development of thrombocytopenia following exposure to the platelet glycoprotein (GP) IIb/ IIIa receptor antagonist abciximab (c7E3 Fab, ReoPro) is associated with adverse clinical outcome and excessive bleeding. Pseudothrombocytopenia is an important differential diagnosis in sudden onset of thrombocytopenia in a ...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s002770050573
更新日期:2000-03-01 00:00:00
abstract::Amifostine increases in vitro burst-forming unit-erythroid and colony-forming unit-granulocyte/granulcoyte-macrophage cultured from bone-marrow cells from patients with myelodysplastic syndrome (MDS). Several small clinical studies give divergent informations about the potential of amifostine as single agent to improv...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s002770050589
更新日期:2000-05-01 00:00:00
abstract::Extramedullary (e) relapse in multiple myeloma(MM) has an adverse prognosis, but knowledge concerning biological features and preferred treatment is scarce. We screened the myeloma registry of our institution for eMM relapses and identified 24 cases among 357 patients (pts).Only 8% of eMM relapses occurred after initi...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-012-1414-5
更新日期:2012-07-01 00:00:00
abstract::An investigation into the effects of irradiation and of the storage time on aging and quality are a relevant issue to ensure the safety and the efficiency of irradiation in the prevention of transfusion-associated graft-versus-host disease (TA-GVHD). In this work, the biochemical properties and alterations presented b...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-013-1944-5
更新日期:2014-05-01 00:00:00
abstract::The appropriate management of patients with moderate aplastic anemia (mAA) remains to be unclear and controversial. A cohort of 118 patients with mAA received a novel immunosuppressive strategy of cyclosporine alternately combined with levamisole (CSA and LMS regimen), which included 42 newly diagnosed and 76 chronic ...
journal_title:Annals of hematology
pub_type: 临床试验,杂志文章
doi:10.1007/s00277-013-1764-7
更新日期:2013-09-01 00:00:00
abstract::Impact of FLT3 receptor tyrosine kinase activation via internal tandem duplication (ITD) of the juxtamembrane region on outcome of acute myeloid leukemia (AML) is still controversial. Recent researches reveal a role of FLT3 in monocyte differentiation in hematopoiesis. We analyzed the clinical impact of FLT3 alteratio...
journal_title:Annals of hematology
pub_type: 杂志文章,多中心研究
doi:10.1007/s00277-009-0733-7
更新日期:2009-11-01 00:00:00
abstract::The objective of this study is to compare and evaluate the diagnostic value of hereditary spherocytosis (HS) by three screening tests, comparing mean spherical corpuscular volume (MSCV) to mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), and flow cytometric osmotic fragility test. Perip...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-014-2270-2
更新日期:2015-05-01 00:00:00
abstract::Over 80% of the α-thalassemia cases in southern China are caused by large deletions involving the α-globin gene cluster on chromosome 16p13.3. Here, we characterized a novel 27.6-kb deletion on the α-globin gene cluster in a Chinese family. Its breakpoints were detected to lie between coordinates 9079 and 36718 of the...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-010-1030-1
更新日期:2011-01-01 00:00:00
abstract::An additional affiliation for the first author was not indicated. Hyewon Lee is also affiliated with: Department of Internal Medicine, Yonsei University College of Medicine, Gangnam Severance Hospital, Seoul, South Korea. ...
journal_title:Annals of hematology
pub_type: 杂志文章,已发布勘误
doi:10.1007/s00277-019-03868-8
更新日期:2020-01-01 00:00:00
abstract::In the context of discussions on the reproducibility of clinical studies, we reanalyzed a prospective randomized study on the role of splenic irradiation as adjunct to the conditioning for hematopoietic stem cell transplantation (HSCT) for chronic myeloid leukemia (CML). Between 1986 and 1989, a total of 229 patients ...
journal_title:Annals of hematology
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00277-016-2638-6
更新日期:2016-05-01 00:00:00
abstract::Rituximab induces B-cell depletion; therefore, it has been used in the treatment of immune thrombocytopenic purpura (ITP). The aim of this retrospective study was to evaluate the effectiveness of rituximab in the treatment of 89 patients with chronic ITP refractory to several treatments. All the patients had platelet ...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-005-0073-1
更新日期:2006-06-01 00:00:00
abstract::Aberrant promoter methylation of RASSF6 and RASSF10 occurs at a high frequency in acute lymphoblastic leukemia (ALL). Because of the complexity of the current minimal residual disease (MRD) detecting-methods, the DNA methylation status of the RASSF6 and RASSF10 genes could potentially become biomarkers for the assessm...
journal_title:Annals of hematology
pub_type: 临床试验,杂志文章
doi:10.1007/s00277-019-03775-y
更新日期:2019-12-01 00:00:00
abstract::It is often difficult for standard blood banks in Korea to supply adequate amounts of blood for patients with rare phenotype. Moreover, the definition of a blood in need is ambiguous, and much remains to be learned. In this study, we determined the prevalence of various red blood cell (RBC) antigens from a donor viewp...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-016-2645-7
更新日期:2016-05-01 00:00:00
abstract::The aim of this study was to evaluate the role of computed tomography (CT)-guided core needle biopsy (CNB) performed by modified coaxial technique as an alternative tool to surgical biopsy in patients with refractory or recurrent lymphomas. Between May 2005 and May 2012, 57 CT-guided CNB of deep lesions were performed...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-014-2078-0
更新日期:2014-09-01 00:00:00
abstract::The present meta-analysis was undertaken to (1) assess erythroid response rates in myelodysplastic syndromes (MDS) patients treated with epoetin alfa as a monotherapy, (2) gain further insights into predictors of response rates, and (3) compare the erythroid response rates observed with epoetin alfa and darbepoetin al...
journal_title:Annals of hematology
pub_type: 杂志文章,meta分析,评审
doi:10.1007/s00277-008-0450-7
更新日期:2008-07-01 00:00:00
abstract::Neutropenic enterocolitis (NE) is a severe gastrointestinal complication in patients who undergo aggressive chemotherapy. It is a necrotizing inflammation of the cecum, colon, and the terminal part of the ileum. The serious clinical state of NE patients requires very frequent surgical consultations; however, in a few ...
journal_title:Annals of hematology
pub_type: 杂志文章,评审
doi:10.1007/s00277-003-0815-x
更新日期:2004-08-01 00:00:00
abstract::The JAK2V617F mutation is part of the major criteria for diagnosis of myeloproliferative neoplasms (MPN). Allele-specific quantitative PCR (qPCR) is the most prevalent method used in laboratories but with the advent of next-generation sequencing (NGS) techniques, we felt necessary to evaluate this approach for JAK2 mu...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-018-3499-y
更新日期:2019-01-01 00:00:00
abstract::Philadelphia-negative myeloproliferative neoplasms (Ph-negative MPN) comprise a heterogeneous group of chronic hematologic malignancies. The quality of life, morbidity, and mortality of patients with MPN are primarily affected by disease-related symptoms, thromboembolic and hemorrhagic complications, and progression t...
journal_title:Annals of hematology
pub_type: 共识发展会议,杂志文章,实务指引,评审
doi:10.1007/s00277-016-2621-2
更新日期:2016-04-01 00:00:00
abstract::von Willebrand disease (VWD) is the commonest inherited bleeding disorder, yet it has not been well recognized in Southeast Asia. The aim of this prospective study was to report our experience of VWD diagnosis and to establish the clinical presentations of VWD in Taiwan. From October 2003 to April 2010, 863 patients w...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-011-1266-4
更新日期:2011-10-01 00:00:00
abstract::High-dose chemotherapy (HDT) and stem cell transplantation is a newer treatment option widely applied in poor-risk germ cell tumor patients. Due to the increasing practical clinical experience and the availability of hematopoietic growth factors, this treatment approach has become a relatively safe procedure. Dependin...
journal_title:Annals of hematology
pub_type: 杂志文章,评审
doi:10.1007/s002770050386
更新日期:1998-05-01 00:00:00
abstract::The purpose of this cross-sectional prospective study was to determine the prevalence of anemia among elderly hospitalized patients in Germany and to investigate its association with multidimensional loss of function (MLF). One hundred participants aged 70 years or older from two distinct wards (50 each from an emerge...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-014-2110-4
更新日期:2014-10-01 00:00:00
abstract::The introduction of rituximab into the primary treatment of malignant lymphomas of the B cell lineage has had a major impact on the management of these diseases. In addition, prolonged exposure to rituximab as maintenance therapy has been beneficial in patients with follicular lymphoma and mantle cell lymphoma. For th...
journal_title:Annals of hematology
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00277-008-0560-2
更新日期:2009-01-01 00:00:00
abstract::We conducted an investigation to clarify whether or not the levels of total, free, and functional protein S and C4-binding protein (C4bp) in plasma are decreased in systemic lupus erythematosus (SLE) patients, especially those with antiphospholipid antibody (aPL), which is known to be a causative factor of such compli...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/BF01696561
更新日期:1994-12-01 00:00:00
abstract::Chronic idiopathic neutropenia of adults (CINA) is a granulocytic disorder characterised by the "unexplained" decrease in the number of circulating neutrophils. Serum inflammatory cytokines and chemokines are increased in CINA. In addition, cytokines gene polymorphisms are associated with increased levels of respectiv...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-005-1066-9
更新日期:2005-10-01 00:00:00
abstract::Methods to estimate bone marrow plasma cells (BMPC) basically include histopathology, cytomorphology, and flow cytometry. The present study compares the outcomes of these methods with special focus on the impact of BMPC-specific characteristics on their recovery by either method. Laboratory reports of diagnostic sampl...
journal_title:Annals of hematology
pub_type: 杂志文章,多中心研究
doi:10.1007/s00277-020-04249-2
更新日期:2020-11-01 00:00:00
abstract::The use of prophylactic antimicrobials during autologous peripheral blood stem cell transplantation (APBSCT) remains controversial. A prospective study was therefore conducted to examine whether the use of prophylactic antimicrobials is necessary. In this study, all the antimicrobials were given therapeutically rather...
journal_title:Annals of hematology
pub_type: 临床试验,杂志文章
doi:10.1007/s00277-002-0567-z
更新日期:2003-01-01 00:00:00
abstract::Parainfluenza virus (PIV) infection is a significant cause of morbidity and mortality, especially in hematologic malignancy patients including hematopoietic stem cell transplantation (HCT) recipients. However, limited information is available for risk stratification in PIV-infected patients with hematologic malignancy...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-020-03996-6
更新日期:2020-06-01 00:00:00