Abstract：
BACKGROUND/AIMS:Hepcidin is a liver-expressed peptide which plays an important role in the regulation of iron metabolism. It is a negative regulator of iron absorption and release of iron from cells. The aims of this study were to analyse the expression and localisation of prohepcidin in liver and cell lines. METHODS:We generated antibodies against recombinant mouse prohepcidin and studied its expression in cell lines, primary hepatocytes and livers of normal mice and mice with abnormalities in iron metabolism. RESULTS:Prohepcidin localised to the secretory pathway, primarily the Golgi apparatus in liver cells and tissues. Hfe and beta2-microglobulin knockout mice have similar levels of prohepcidin protein expression as compared to wild-type mice despite increased iron stores. Sex-linked anaemia mice have iron deficiency and no prohepcidin expression in the liver. CONCLUSIONS:Prohepcidin protein is present in the secretory pathway of liver cells. Despite iron loading, mouse models of haemochromatosis have comparatively normal levels of prohepcidin expression whereas mice with iron deficiency have no prohepcidin expression.
journal_name
J Hepatoljournal_title
Journal of hepatologyauthors
Wallace DF,Summerville L,Lusby PE,Subramaniam VNdoi
10.1016/j.jhep.2005.02.047subject
Has Abstractpub_date
2005-10-01 00:00:00pages
720-8issue
4eissn
0168-8278issn
1600-0641pii
S0168-8278(05)00286-2journal_volume
43pub_type
杂志文章abstract：BACKGROUND/AIMS:We conducted an analysis of chromosomal numerical aberrations and their clinical significance in hepatocellular carcinoma. METHODS:We analyzed 87 hepatocellular carcinomas by array-based comparative genomic hybridization with an array containing 800 bacterial artificial chromosome clones. RESULTS:Freq...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2005.05.033
更新日期：2005-11-01 00:00:00
abstract：BACKGROUND/AIMS:Cirrhotic livers exhibit endothelial dysfunction that contributes to the increased hepatic vascular resistance. The present study evaluates the role of cyclooxygenase (COX)-derived prostanoids, implicated in the pathogenesis of endothelial dysfunction in other settings, in the pathogenesis of endothelia...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(03)00347-7
更新日期：2003-10-01 00:00:00
abstract：BACKGROUND & AIMS:Genetic background may affect liver damage in patients with non-alcoholic fatty liver disease (NAFLD). The main outcomes of the study were to assess whether IL28B rs12979860 and rs8099917 polymorphisms, together with PNPLA3 rs738409 C>G polymorphism, are associated with lobular inflammation and fibros...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2012.01.007
更新日期：2012-06-01 00:00:00
abstract：BACKGROUND/AIMS:Non-alcoholic fatty liver disease (NAFLD) pathogenesis remains unknown. Due to the emerging role of free cholesterol (FC) in NAFLD, our aim was to examine the correlation between FC accumulation in patients with NAFLD and the expression of enzymes that regulate cholesterol homeostasis. METHODS:Filipin ...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2008.12.016
更新日期：2009-04-01 00:00:00
abstract：BACKGROUND/AIMS:Modifications of the Child-Pugh classification of liver cirrhosis by incorporation of hyaluronan were tested to improve the prognostic power for long term evaluation of liver cirrhosis in 126 patients observed over a period of 10 years. METHODS:Serum concentrations of HA were determined at study entry....
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(03)00431-8
更新日期：2003-12-01 00:00:00
abstract：BACKGROUND & AIMS:Adherence to antiviral treatment is important to achieve sustained virological response (SVR) in chronic hepatitis C (CHC). We evaluated the efficiency of a multidisciplinary support programme (MSP), based on published HIV treatment experience, to increase patient adherence and the efficacy of pegylat...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2013.06.019
更新日期：2013-11-01 00:00:00
abstract：:Since the first tests for antibodies to components of the hepatitis C virus became widely available there has been considerable interest in evidence linking HCV infection with autoimmune liver diseases and other autoimmune conditions. With respect to autoimmune hepatitis, it is now clear that the early tests were quit...
journal_title：Journal of hepatology
pub_type： 杂志文章,评审
doi：
更新日期：1996-01-01 00:00:00
abstract：:A monoclonal antibody, designated K-PLC1, has been produced to a tumour-associated antigen on the cell membrane of the PLC/PRF/5 cell line. Using an indirect immunofluorescence technique this antibody produced membrane staining of three hepatocellular carcinoma (HCC) cell lines and it has positively stained 10 of 11 h...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(87)80009-0
更新日期：1987-02-01 00:00:00
abstract：:To study experimentally the protective effect of post-exposure prophylaxis against hepatitis B (HB), a special preparation of hepatitis B immune globulin (HBIG) was injected intravenously (i.v.) into three chimpanzees simultaneously with, or at different time intervals after, intravenous injection of a titred inoculum...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/0168-8278(89)90051-2
更新日期：1989-09-01 00:00:00
abstract：BACKGROUND/AIMS:Interaction between CYP2E1, ethanol metabolites, and enhanced lipid peroxidation is linked to the pathogenesis of alcoholic liver disease. This study was conducted to compare the expression of various cytochrome enzymes and the appearance of aldehyde adducts in humans. METHODS:Acetaldehyde- and lipid p...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(00)80120-8
更新日期：2000-12-01 00:00:00
abstract：BACKGROUND/AIMS:Fulminant hepatic failure (FHF) can be dreadful. When coma sets in, brain edema develops taking FHF into a lethal course. Mechanisms of brain extravasation leading to brain edema remain incompletely understood. Matrix metalloproteinase (MMP)-9 is implicated in various brain injuries. We hypothesized tha...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2005.09.019
更新日期：2006-06-01 00:00:00
abstract：BACKGROUND/AIMS:Hepatitis C has been associated with a decrease in quality of life and with neurological abnormalities. The aim of our study was to investigate the relationship between quality of life and cognitive function. METHODS:Quality of life, clinical variables and neuropsychological function were evaluated in ...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(03)00189-2
更新日期：2003-08-01 00:00:00
abstract：BACKGROUND/AIMS:We assessed mitochondrial morphology by electron microscopy and the prevalence of a mitochondrial gene deletion in patients with non-alcoholic steatohepatitis (NASH), alcohol-related liver disease and non-fatty liver diseases. Respiratory chain function using a cytoplasmic hybrid (cybrid) assay was furt...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(99)80033-6
更新日期：1999-09-01 00:00:00
abstract：:We report a case of chronic active hepatitis caused by benzarone, a benzofuran derivative used in Europe for the treatment of peripheral venous disorders. Jaundice and serum alanine aminotransferase activity increased while drug administration was continued, but promptly decreased when it was eventually interrupted. L...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(87)80039-9
更新日期：1987-12-01 00:00:00
abstract：BACKGROUND & AIMS:Although there is increasing interest in its use, definitive evidence demonstrating a benefit for postmortem normothermic regional perfusion (NRP) in controlled donation after circulatory death (cDCD) liver transplantation is lacking. The aim of this study was to compare results of cDCD liver transpla...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2018.12.013
更新日期：2019-04-01 00:00:00
abstract：BACKGROUND & AIMS:Biliary atresia represents obstructive cholangiopathy in infants progressing rapidly to cirrhosis and end-stage liver disease. Activated NK cells expressing Nkg2d have been linked to bile duct injury and obstruction by establishing contact with cholangiocytes. To define the mechanisms used by cytotoxi...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2013.09.021
更新日期：2014-02-01 00:00:00
abstract：:Tissue stress and cell death result in inflammation even in the absence of pathogens. Such sterile inflammation is dependent on a cytosolic complex of proteins inside immune cells termed the inflammasome. This complex converts two groups of extracellular signals into an inflammatory response via activation of caspase-...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2012.12.017
更新日期：2013-05-01 00:00:00
abstract：BACKGROUND & AIMS:The mechanisms of treatment resistance to interferon (IFN) and ribavirin (Rib) combination therapy for hepatitis C virus (HCV) infection are not known. This study aims to gain insight into these mechanisms by exploring hepatic gene expression before and during treatment. METHODS:Liver biopsy was perf...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2010.04.036
更新日期：2010-11-01 00:00:00
abstract：:The prolylhydroxylase inhibitor HOE 077 which has proven to be liver-selective in animals was tested in general pharmacological and toxicological studies for unwanted effects and safety. The results of these studies permit clinical development. A single-dose study in healthy volunteers was performed which showed that ...
journal_title：Journal of hepatology
pub_type： 杂志文章,评审
doi：10.1016/0168-8278(91)90009-z
更新日期：1991-01-01 00:00:00
abstract：BACKGROUND & AIMS:Recently, we have shown that micro-metastases, in the hypoxic transition zone surrounding lesions generated by radiofrequency ablation (RFA), display strongly accelerated outgrowth. CD95 is best known for its ability to induce apoptosis but can also promote tumorigenesis in apoptosis-resistant tumor c...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2010.04.040
更新日期：2010-12-01 00:00:00
abstract：BACKGROUND/AIMS:In order to explore the role of cytokines in the pathogenesis of liver cirrhosis, we analyzed their gene expression in hepatic biopsies from patients with alcoholic liver cirrhosis, post-hepatitis C liver cirrhosis, and with idiopathic portal hypertension without cirrhosis. METHODS:We assessed the gene...
journal_title：Journal of hepatology
pub_type： 临床试验,杂志文章
doi：10.1016/s0168-8278(96)80140-1
更新日期：1996-05-01 00:00:00
abstract：:The activation of protein kinase C (PKC) has been implicated in the pathogenesis of gram-negative sepsis. The effects of PKC modulation on hepatic flow and metabolism were studied using isolated liver perfusion. The liver was isolated from well-fed or overnight-fasted, male Sprague-Dawley rats weighing 250-310 g, and ...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(05)80561-6
更新日期：1993-11-01 00:00:00
abstract：BACKGROUND & AIMS:Weak hepatitis C virus (HCV) specific immunity in peripheral blood has been shown to be partially controlled by regulatory T cells (Treg). However, little is known about Treg present in livers of HCV-infected patients, their association with clinical parameters, and immunopathology resulting in diseas...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2009.12.013
更新日期：2010-03-01 00:00:00
abstract：:Hepatitis C virus (HCV) infection is associated with tremendous morbidity and mortality due to liver complications. HCV infection is also associated with many extrahepatic manifestations including cardiovascular diseases, glucose metabolism impairment, cryoglobulinemia vasculitis, B cell non-Hodgkin lymphoma and chron...
journal_title：Journal of hepatology
pub_type： 杂志文章,评审
doi：10.1016/j.jhep.2016.06.011
更新日期：2016-10-01 00:00:00
abstract：BACKGROUND & AIMS:Hepatocyte-like cells, differentiated from different stem cell sources, are considered to have a range of possible therapeutic applications, including drug discovery, metabolic disease modelling, and cell transplantation. However, little is known about how stem cells differentiate into mature and func...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2014.07.025
更新日期：2014-12-01 00:00:00
abstract：BACKGROUND & AIMS:There is no data to suggest that the size of bile ducts, portal venules, and hepatic arterioles varies according to age in the normal human liver. We sought to examine whether hepatic arteriolar size, wall thickness, and luminal diameter change with increasing age. METHODS:Histologically normal liver...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2010.12.018
更新日期：2011-09-01 00:00:00
abstract：BACKGROUND/AIMS:We have developed a gene therapy strategy based on the observation that insulin-like growth factor I (IGF-I) is necessary for the acquisition and maintenance of the transformed phenotype in hepatocarcinoma. This strategy consists in transfecting the rat hepatoma cell line with an episomal vector express...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(98)80263-8
更新日期：1998-11-01 00:00:00
abstract：:Current medical practice artificially dichotomises a diagnosis of fatty liver disease into one of two common forms: alcoholic liver disease (ALD) and non-alcoholic fatty liver disease (NAFLD). Together, these account for the majority of chronic liver diseases worldwide. In recent years, there has been a dramatic incre...
journal_title：Journal of hepatology
pub_type： 杂志文章,评审
doi：10.1016/j.jhep.2017.11.006
更新日期：2018-02-01 00:00:00
abstract：BACKGROUND & AIMS:Sustained viral response (SVR) after therapy with interferon-ribavirin (IF-RB) reduces liver-related (LR) complications and mortality in HIV/HCV-co-infected patients. Here, we assess the impact of end-of-treatment response with subsequent relapse (REL) on LR events (LR death, liver decompensation, hep...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2013.01.042
更新日期：2013-06-01 00:00:00
abstract：BACKGROUND & AIMS:Diabetes occurring as a direct consequence of loss of liver function is usually characterized by non-diabetic fasting plasma glucose (FPG) and haemoglobin A1c (HbA1c) levels and should regress after orthotopic liver transplantation (OLT). This observational, longitudinal study investigated the relatio...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2019.01.015
更新日期：2019-05-01 00:00:00
