当前位置： SCI文献检索 > JOURNAL OF HEPATOLOGY期刊下所有文献 > Induction of apoptosis in rat hepatocarcinoma cells by expression of IGF-I antisense c-DNA.

Induction of apoptosis in rat hepatocarcinoma cells by expression of IGF-I antisense c-DNA.

Abstract：

BACKGROUND/AIMS:We have developed a gene therapy strategy based on the observation that insulin-like growth factor I (IGF-I) is necessary for the acquisition and maintenance of the transformed phenotype in hepatocarcinoma. This strategy consists in transfecting the rat hepatoma cell line with an episomal vector expressing the antisense IGF-I c-DNA under the control of the metallothionein I promoter inducible by zinc, decreasing therefore the level of IGF-I in these cells. The transfected clones lost their tumorigenic properties, and were able to induce, in vivo, the regression of an established tumor in syngeneic rats. To understand the loss of tumorigenic properties of these transfected clones, we have quantified, by different approaches, the number of apoptotic cells according to the level of IGF-I expression. METHODS:IGF-I antisense synthesis in transfected cells was stimulated using zinc. We then characterized and quantified apoptosis, in these transfected clones, by morphological and DNA fragmentation analyses, flow cytometry and comet assay. RESULTS:We have demonstrated that IGF-I inhibits the development of apoptosis in parental cells, that the transfected clones are able to restore the spontaneous apoptotic programme, and that apoptosis increases massively when overexpression of IGF-I antisense is caused by zinc stimulation of the metallothionein I promoter. CONCLUSION:The present results allow us to conclude that the level of apoptotic pathway in liver cell lines is directly related to the amount of IGF-I deficiency.

journal_name

J Hepatol

journal_title

Journal of hepatology

authors

Ellouk-Achard S,Djenabi S,De Oliveira GA,Desauty G,Duc HT,Zohair M,Trojan J,Claude JR,Sarasin A,Lafarge-Frayssinet C

doi

10.1016/s0168-8278(98)80263-8

subject

Has Abstract

pub_date

1998-11-01 00:00:00

pages

807-18

issue

5

eissn

0168-8278

issn

1600-0641

pii

S0168-8278(98)80263-8

journal_volume

29

pub_type

杂志文章

相关文献

JOURNAL OF HEPATOLOGY文献大全
  • Oxidative stress induces the endoplasmic reticulum stress and facilitates inclusion formation in cultured cells.

    abstract：BACKGROUND/AIMS:The precise mechanism of formation and significance of Mallory bodies (MBs) are poorly understood. The endoplasmic reticulum (ER) is the organelle responsible for proper folding and elimination of unfolded proteins. Therefore, failure of this function increases defective proteins in the cell. METHODS:W...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/j.jhep.2007.01.039

    authors： Hanada S,Harada M,Kumemura H,Bishr Omary M,Koga H,Kawaguchi T,Taniguchi E,Yoshida T,Hisamoto T,Yanagimoto C,Maeyama M,Ueno T,Sata M

    更新日期：2007-07-01 00:00:00

  • Efficacy and safety of oral naltrexone treatment for pruritus of cholestasis, a crossover, double blind, placebo-controlled study.

    abstract：BACKGROUND/AIMS:To assess the efficacy and safety of naltrexone for the short and long term treatment of pruritus of cholestasis. METHODS:Twenty patients with pruritus and cholestasis were included. A baseline pruritus score was obtained over 1 week. Patients were then randomized to receive 50 mg/day of naltrexone or ...

    journal_title：Journal of hepatology

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1016/s0168-8278(02)00318-5

    authors： Terg R,Coronel E,Sordá J,Muñoz AE,Findor J

    更新日期：2002-12-01 00:00:00

  • Thrombocytopenia post liver transplantation. Correlations with pre-operative platelet count, blood transfusion requirements, allograft function and outcome.

    abstract：:This study reports that thrombocytopenia is a universal phenomenon post hepatic transplantation. In 53 consecutive adult patients undergoing liver transplantation the platelet count fell by a mean of 63% (157 x 10(9)/l to 50 x 10(9)/l). The platelet count reached a nadir at Day 5 post-transplant but returned to pre-op...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/s0168-8278(05)80089-3

    authors： McCaughan GW,Herkes R,Powers B,Rickard K,Gallagher ND,Thompson JF,Sheil AG

    更新日期：1992-09-01 00:00:00

  • Mechanisms of HBV-related hepatocarcinogenesis.

    abstract：:The hepatitis B virus (HBV) is a small enveloped DNA virus, which primarily infects hepatocytes and causes acute and persistent liver disease. Epidemiological studies have provided overwhelming evidence for a causal role of chronic HBV infection in the development of hepatocellular carcinoma, but the molecular mechani...

    journal_title：Journal of hepatology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jhep.2009.10.033

    authors： Neuveut C,Wei Y,Buendia MA

    更新日期：2010-04-01 00:00:00

  • Presence of HBV-DNA in peripheral blood mononuclear cells from anti-HIV symptomless carriers.

    abstract：:The presence of hepatitis B virus DNA (HBV-DNA) in the peripheral blood mononuclear cells (PBMC) of 29 anti-HIV symptomless carriers (eleven HBeAg positive, eleven anti-HBe positive and seven HBsAg negative) and of 40 anti-human immunodeficiency virus (HIV)-negative patients (15 HBeAg positive, 15 anti-HBe positive an...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/0168-8278(90)90050-2

    authors： Bartolomé FJ,Moraleda G,Castillo I,Martinez MG,Porres JC,Carreño V

    更新日期：1990-03-01 00:00:00

  • Putrescine decreases cytochrome P450 3A4 levels during liver regeneration in the rat.

    abstract：BACKGROUND/AIMS:The mechanism by which many cytochrome P450 (CYP) isozymes decrease during liver regeneration is unclear. Peptides and growth factors are thought to be involved. Putrescine, the first polyamine synthesised by ornithine decarboxylase, peaks early following partial hepatectomy and is known to play an esse...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/s0168-8278(98)80295-x

    authors： Favre C,Monti JA,Scapini C,Pellegrino J,Carnovale CE,Carrillo MC

    更新日期：1998-04-01 00:00:00

  • Soft drink consumption is associated with fatty liver disease independent of metabolic syndrome.

    abstract：BACKGROUND/AIMS:The independent role of soft drink consumption in non-alcoholic fatty liver disease (NAFLD) patients remains unclear. We aimed to assess the association between consumption of soft drinks and fatty liver in patients with or without metabolic syndrome. METHODS:We recruited 31 patients (age: 43+/-12 year...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/j.jhep.2009.05.033

    authors： Abid A,Taha O,Nseir W,Farah R,Grosovski M,Assy N

    更新日期：2009-11-01 00:00:00

  • Alternatively activated macrophages promote resolution of necrosis following acute liver injury.

    abstract：BACKGROUND & AIM:Following acetaminophen (APAP) overdose, acute liver injury (ALI) can occur in patients that present too late for N-acetylcysteine treatment, potentially leading to acute liver failure, systemic inflammation, and death. Macrophages influence the progression and resolution of ALI due to their innate imm...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/j.jhep.2020.02.031

    authors： Starkey Lewis P,Campana L,Aleksieva N,Cartwright JA,Mackinnon A,O'Duibhir E,Kendall T,Vermeren M,Thomson A,Gadd V,Dwyer B,Aird R,Man TY,Rossi AG,Forrester L,Park BK,Forbes SJ

    更新日期：2020-08-01 00:00:00

  • Non-organ specific autoantibodies associated with chronic C virus hepatitis.

    abstract：:Recently antibodies to hepatitis C virus were detected in sera of chronic active hepatitis patients, with anti-smooth muscle autoantibodies or with anti-liver/kidney microsomal type 1 autoantibodies. As the latter were used to differentiate autoimmune chronic active hepatitis from chronic non-A, non-B virus hepatitis,...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/s0168-8278(05)80281-8

    authors： Abuaf N,Lunel F,Giral P,Borotto E,Laperche S,Poupon R,Opolon P,Huraux JM,Homberg JC

    更新日期：1993-07-01 00:00:00

  • Identification of naïve HCV-1 patients with chronic hepatitis who may benefit from dual therapy with peg-interferon and ribavirin.

    abstract：BACKGROUND & AIMS:The pool of HCV genotype 1 patients likely to be cured by peg-interferon and ribavirin remains to be quantified. METHODS:In 1045 patients treated with peg-interferon and ribavirin, two therapeutic strategies were confronted: the first one evaluated only baseline variables associated with sustained vi...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/j.jhep.2013.07.040

    authors： Andriulli A,Di Marco V,Margaglione M,Ippolito AM,Fattovich G,Smedile A,Valvano MR,Calvaruso V,Gioffreda D,Milella M,Morisco F,Felder M,Brancaccio G,Fasano M,Gatti P,Tundo P,Barone M,Cozzolongo R,Angelico M,D'Andrea

    更新日期：2014-01-01 00:00:00

  • Deterioration of health-related quality of life and fatigue in patients with chronic hepatitis C: Association with demographic factors, inflammatory activity, and degree of fibrosis.

    abstract：BACKGROUND/AIMS:Health-related quality of life (HRQoL) is impaired in patients with chronic hepatitis C. We investigated HRQoL and fatigue in patients with chronic hepatitis C virus (HCV) infection in relation to the degree of fibrosis and inflammation, and controlled for the influence of relevant demographic and medic...

    journal_title：Journal of hepatology

    pub_type： 杂志文章,多中心研究

    doi：10.1016/j.jhep.2008.07.025

    authors： Teuber G,Schäfer A,Rimpel J,Paul K,Keicher C,Scheurlen M,Zeuzem S,Kraus MR

    更新日期：2008-12-01 00:00:00

  • Intrahepatic IL-8 producing Foxp3⁺CD4⁺ regulatory T cells and fibrogenesis in chronic hepatitis C.

    abstract：BACKGROUND & AIMS:Regulatory CD4(+) T cells (Tregs) are considered to affect outcomes of HCV infection, because they increase in number during chronic hepatitis C and can suppress T-cell functions. METHODS:Using microarray analysis, in situ immunofluorescence, ELISA, and flowcytometry, we characterised functional diff...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/j.jhep.2013.04.011

    authors： Langhans B,Krämer B,Louis M,Nischalke HD,Hüneburg R,Staratschek-Jox A,Odenthal M,Manekeller S,Schepke M,Kalff J,Fischer HP,Schultze JL,Spengler U

    更新日期：2013-08-01 00:00:00

  • Aquaretic agents: a new potential treatment of dilutional hyponatremia in cirrhosis.

    abstract：:An impairment in the renal capacity to excrete water is a common finding in patients with cirrhosis and ascites. In some patients this abnormality is minor since it is only detectable by measuring urine volume or free water clearance after a water load and is not associated with changes in plasma osmolality and serum ...

    journal_title：Journal of hepatology

    pub_type： 杂志文章,评审

    doi：10.1016/s0168-8278(96)80174-7

    authors： Ginès P,Jiménez W

    更新日期：1996-04-01 00:00:00

  • Gα12 overexpression induced by miR-16 dysregulation contributes to liver fibrosis by promoting autophagy in hepatic stellate cells.

    abstract：BACKGROUND & AIMS:Hepatic stellate cells (HSCs) have a role in liver fibrosis. Guanine nucleotide-binding α-subunit 12 (Gα12) converges signals from G-protein-coupled receptors whose ligand levels are elevated in the environment during liver fibrosis; however, information is lacking on the effect of Gα12 on HSC trans-d...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/j.jhep.2017.10.011

    authors： Kim KM,Han CY,Kim JY,Cho SS,Kim YS,Koo JH,Lee JM,Lim SC,Kang KW,Kim JS,Hwang SJ,Ki SH,Kim SG

    更新日期：2018-03-01 00:00:00

  • The major histocompatibility complex influences the development of chronic liver disease in male children and young adults with cystic fibrosis.

    abstract：BACKGROUND/AIMS:Chronic liver disease is a well-recognised complication of cystic fibrosis. Recent reports suggest that its development is not determined by specific mutations within the cystic fibrosis gene; however, familial clustering of portal hypertension cases and inappropriate immune responses against liver memb...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/0168-8278(95)80058-1

    authors： Duthie A,Doherty DG,Donaldson PT,Scott-Jupp R,Tanner MS,Eddleston AL,Mowat AP

    更新日期：1995-11-01 00:00:00

  • Advances in functional and molecular MRI technologies in chronic liver diseases.

    abstract：:MRI has emerged as the most comprehensive non-invasive diagnostic tool for liver diseases. In recent years, the value of MRI in hepatology has been significantly enhanced by a wide range of contrast agents, both clinically available and under development, that add functional information to anatomically detailed morpho...

    journal_title：Journal of hepatology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jhep.2020.06.020

    authors： Zhou IY,Catalano OA,Caravan P

    更新日期：2020-11-01 00:00:00

  • Therapeutic management of hepatitis and HIV infection in co-infected patients: results of a survey performed before the 2005 Consensus Conference.

    abstract：:The First European Consensus Conference on the Treatment of Chronic Hepatitis B and C in HIV Co-infected Patients aimed to produce recommendations that could be applied across Europe. However, some important differences exist around Europe, in terms of access to treatment and tests for monitoring. This short survey of...

    journal_title：Journal of hepatology

    pub_type： 杂志文章,多中心研究

    doi：10.1016/j.jhep.2005.11.034

    authors： Salmon D,Robain M,Rockstroh JK,Benhamou Y

    更新日期：2006-01-01 00:00:00

  • Evidence for a link between hepatitis C virus infection and diabetes mellitus in a cirrhotic population.

    abstract：:Abnormalities of carbohydrate metabolism, including hyperinsulinaemia and insulin resistance, are well recognised complications of cirrhosis. While diabetes mellitus can be explained in many instances on the basis of coincident pancreatic disease, in most the characteristic glucose intolerance of cirrhosis is not read...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/s0168-8278(05)80631-2

    authors： Allison ME,Wreghitt T,Palmer CR,Alexander GJ

    更新日期：1994-12-01 00:00:00

  • Characterisation of citrate and iron citrate uptake by cultured rat hepatocytes.

    abstract：BACKGROUND/AIMS:The endogenous low molecular weight iron chelator, citrate, is considered to be an important contributor to iron transport and the liver the main site of uptake of iron citrate in subjects suffering from diseases of iron overload. Moreover, the citrate-metabolising enzyme, aconitase, is implicated in th...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/s0168-8278(98)80156-6

    authors： Graham RM,Morgan EH,Baker E

    更新日期：1998-10-01 00:00:00

  • Natural history and outcome in 32 Swedish patients with small duct primary sclerosing cholangitis (PSC).

    abstract：BACKGROUND/AIMS:This study aims at describing the natural history and outcome of small duct primary sclerosing cholangitis (PSC). METHODS:Thirty-two patients with small duct PSC were studied. The average time taken for diagnosis was 69 (1-168) months. The median follow-up time was 63 (1-194) months. RESULTS:All patie...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/s0168-8278(02)00036-3

    authors： Broomé U,Glaumann H,Lindstöm E,Lööf L,Almer S,Prytz H,Sandberg-Gertzén H,Lindgren S,Fork FT,Järnerot G,Olsson R

    更新日期：2002-05-01 00:00:00

  • CD95 is a key mediator of invasion and accelerated outgrowth of mouse colorectal liver metastases following radiofrequency ablation.

    abstract：BACKGROUND & AIMS:Recently, we have shown that micro-metastases, in the hypoxic transition zone surrounding lesions generated by radiofrequency ablation (RFA), display strongly accelerated outgrowth. CD95 is best known for its ability to induce apoptosis but can also promote tumorigenesis in apoptosis-resistant tumor c...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/j.jhep.2010.04.040

    authors： Nijkamp MW,Hoogwater FJ,Steller EJ,Westendorp BF,van der Meulen TA,Leenders MW,Borel Rinkes IH,Kranenburg O

    更新日期：2010-12-01 00:00:00

  • Toll-like receptor 3 downregulation is an escape mechanism from apoptosis during hepatocarcinogenesis.

    abstract：BACKGROUND & AIMS:Low levels of toll-like receptor 3 (TLR3) in patients with hepatocellular carcinoma (HCC) are associated with poor prognosis, primarily owing to the loss of inflammatory signaling and subsequent lack of immune cell recruitment to the liver. Herein, we explore the role of TLR3-triggered apoptosis in HC...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/j.jhep.2019.05.031

    authors： Bonnin M,Fares N,Testoni B,Estornes Y,Weber K,Vanbervliet B,Lefrançois L,Garcia A,Kfoury A,Pez F,Coste I,Saintigny P,Viari A,Lang K,Guey B,Hervieu V,Bancel B,Bartoch B,Durantel D,Renno T,Merle P,Lebecque S

    更新日期：2019-10-01 00:00:00

  • A predictive model of treatment outcome in patients with chronic HCV infection using IL28B and PD-1 genotyping.

    abstract：BACKGROUND & AIMS:The advent of new chronic hepatitis C virus (HCV) therapies requires characterization of patients in order to predict adequate treatment. A good candidate marker is Programmed Cell Death-1 (PD-1) which is involved in progression of HCV infection. The aim of this study was to analyse the effect of seve...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/j.jhep.2012.01.011

    authors： Vidal-Castiñeira JR,López-Vázquez A,Alonso-Arias R,Moro-García MA,Martinez-Camblor P,Melón S,Prieto J,López-Rodriguez R,Sanz-Cameno P,Rodrigo L,Pérez-López R,Pérez-Álvarez R,López-Larrea C

    更新日期：2012-06-01 00:00:00

  • Hedgehog signaling in the liver.

    abstract：:Reactivation of Hedgehog (Hh), a morphogenic signaling pathway that controls progenitor cell fate and tissue construction during embryogenesis occurs during many types of liver injury in adult. The net effects of activating the Hedgehog pathway include expansion of liver progenitor populations to promote liver regener...

    journal_title：Journal of hepatology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jhep.2010.10.003

    authors： Omenetti A,Choi S,Michelotti G,Diehl AM

    更新日期：2011-02-01 00:00:00

  • Prevalence and prognostic value of quantified electroencephalogram (EEG) alterations in cirrhotic patients.

    abstract：BACKGROUND/AIMS:The electroencephalogram (EEG) is frequently altered in cirrhotic patients. We, therefore, performed a study to ascertain the features and the prognosis of cirrhotic patients without current overt hepatic encephalopathy (OHE) who have EEG alterations. METHODS:A series of 296 consecutive cirrhotic patie...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/s0168-8278(01)00129-5

    authors： Amodio P,Del Piccolo F,Pettenò E,Mapelli D,Angeli P,Iemmolo R,Muraca M,Musto C,Gerunda G,Rizzo C,Merkel C,Gatta A

    更新日期：2001-07-01 00:00:00

  • Cytokine gene expression in cirrhotic and non-cirrhotic human liver.

    abstract：BACKGROUND/AIMS:In order to explore the role of cytokines in the pathogenesis of liver cirrhosis, we analyzed their gene expression in hepatic biopsies from patients with alcoholic liver cirrhosis, post-hepatitis C liver cirrhosis, and with idiopathic portal hypertension without cirrhosis. METHODS:We assessed the gene...

    journal_title：Journal of hepatology

    pub_type： 临床试验,杂志文章

    doi：10.1016/s0168-8278(96)80140-1

    authors： Llorent L,Richaud-Patin Y,Alcocer-Castillejos N,Ruiz-Soto R,Mercado MA,Orozco H,Gamboa-Domínguez A,Alcocer-Varela J

    更新日期：1996-05-01 00:00:00

  • Phenprocoumon-induced liver disease ranges from mild acute hepatitis to (sub-) acute liver failure.

    abstract：BACKGROUND/AIMS:Except for bleeding complications, other serious adverse reactions of coumarin anticoagulants such as hepatotoxicity or skin necrosis are comparatively rare. Nonetheless, a small number of coumarin-induced (sub-) acute liver failures has been published. METHODS:A retrospective analysis was performed of...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/j.jhep.2004.03.010

    authors： Schimanski CC,Burg J,Möhler M,Höhler T,Kanzler S,Otto G,Galle PR,Lohse AW

    更新日期：2004-07-01 00:00:00

  • TGF-β-dependent induction of CD4⁺CD25⁺Foxp3⁺ Tregs by liver sinusoidal endothelial cells.

    abstract：BACKGROUND & AIMS:CD4(+) CD25(+) Foxp3(+) regulatory T cells (Tregs) have a profound ability to control immune responses. We have previously shown that the liver is a major source of peripherally induced Tregs. Here, we investigate the liver cell types and molecular mechanisms responsible for hepatic Treg induction. M...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/j.jhep.2014.04.027

    authors： Carambia A,Freund B,Schwinge D,Heine M,Laschtowitz A,Huber S,Wraith DC,Korn T,Schramm C,Lohse AW,Heeren J,Herkel J

    更新日期：2014-09-01 00:00:00

  • An outbreak of hepatitis A virus infection with a high case-fatality rate among injecting drug users.

    abstract：BACKGROUND/AIMS:In 2002, the first reported outbreak of hepatitis A virus (HAV) infection involving mostly intravenous drug users (IDU) occurred in Italy. We attempted a thorough evaluation of the outbreak, including epidemiological, clinical and virological analyses. METHODS:We conducted an epidemiological investigat...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/j.jhep.2005.06.012

    authors： Spada E,Genovese D,Tosti ME,Mariano A,Cuccuini M,Proietti L,Giuli CD,Lavagna A,Crapa GE,Morace G,Taffon S,Mele A,Rezza G,Rapicetta M

    更新日期：2005-12-01 00:00:00

  • Normal pharmacologically-induced, but decreased regenerative liver growth in interleukin-6-deficient (IL-6(-/-)) mice.

    abstract：BACKGROUND/AIMS:In the absence of liver damage, rapid liver growth can be induced pharmacologically by so-called primary liver growth promoters. The importance of the acute-phase cytokines interleukin-6 and tumor necrosis factor-alpha for the actions of these compounds is not clear. This study aimed to investigate the ...

    journal_title：Journal of hepatology

    pub_type： 杂志文章

    doi：10.1016/s0168-8278(00)80130-0

    authors： Wallenius V,Wallenius K,Jansson JO

    更新日期：2000-12-01 00:00:00