Abstract：
BACKGROUND/AIMS:The endogenous low molecular weight iron chelator, citrate, is considered to be an important contributor to iron transport and the liver the main site of uptake of iron citrate in subjects suffering from diseases of iron overload. Moreover, the citrate-metabolising enzyme, aconitase, is implicated in the regulation of cellular iron metabolism. This study was undertaken to determine the role of citrate and ferric citrate in the uptake of iron by rat hepatocytes. METHODS:Cultured rat hepatocytes were incubated (37 degrees C, 15 min) with 100 microM [14C]-citrate in the presence or absence of 1.0 microM 55Fe. Membrane-bound and intracellular radiolabel were separated by incubation with the general protease, Pronase. RESULTS:Our results suggest that ferric citrate uptake is mediated by a specific citrate binding site which exhibits a higher affinity for citrate in the presence of iron than in its absence. Citrate was internalised by hepatocytes, with at least 70% being oxidised to CO2 within 15 min. Citrate uptake was pH-dependent, did not require the presence of sodium and increased with increasing iron concentration. Metabolic energy, anion channels, the Na+, K+-ATPase and vesicle acidification do not appear to play a role in uptake of ferric citrate, but functional sulphydryl groups may be involved. CONCLUSIONS:The data suggest either that ferric citrate complexes with higher molar ratios of iron to citrate relative to the incubation medium are bound preferentially to the membrane, or that once citrate has delivered its iron to the membrane, the complex dissociates and the components are internalised separately.
journal_name
J Hepatoljournal_title
Journal of hepatologyauthors
Graham RM,Morgan EH,Baker Edoi
10.1016/s0168-8278(98)80156-6subject
Has Abstractpub_date
1998-10-01 00:00:00pages
603-13issue
4eissn
0168-8278issn
1600-0641pii
S0168-8278(98)80156-6journal_volume
29pub_type
杂志文章abstract：:In an attempt to evaluate the prevalence, kinetics and impact of HCV infection in renal transplantation, we analyzed 140 kidney recipients according to the histopathological status of the liver. Thirty-three HBsAg-negative patients had chronic active hepatitis, 73 HBsAg-negative patients had a normal liver, 21 HBsAg-n...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/0168-8278(92)90036-o
更新日期：1992-05-01 00:00:00
abstract：:Lactitol is a disaccharide analogue of lactulose which is available as a pure crystalline powder. The efficacy of lactitol in the treatment of chronic hepatic encephalopathy was assessed in 9 cirrhotic patients in a randomised, double-blind, cross-over comparison with lactulose. The sugars were dispensed in solutions,...
journal_title：Journal of hepatology
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0168-8278(87)80086-7
更新日期：1987-04-01 00:00:00
abstract：:Epstein-Barr virus infection is a benign disease, which may occasionally be fatal, particularly in children. Epstein-Barr virus infection is rare in elderly subjects and appears to have a self-limited course. An unusual case of fulminant hepatitis due to primary Epstein-Barr virus infection in a 62-year-old male 18 da...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：
更新日期：1995-09-01 00:00:00
abstract：BACKGROUND & AIMS:Reactive cholangiocytes acquire a neuroendocrine-like phenotype, with synthesis and local release of neuropeptides and hormones. The mechanism that drives such phenotypical changes is still undefined. Pancreatic Duodenal Homeobox-1 (PDX-1) is a transcription factor required for pancreatic development,...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2010.04.022
更新日期：2010-10-01 00:00:00
abstract：BACKGROUND/AIM:Anti-neutrophil cytoplasmic antibodies (ANCA) are a group of autoantibodies first associated with Wegener's granulomatosis and microscopic polyangiitis. The significance of ANCA in autoimmune hepatitis remains uncertain; the nature of the antigen or antigens has not been defined yet. The purpose of this ...
journal_title：Journal of hepatology
pub_type： 临床试验,杂志文章
doi：10.1016/s0168-8278(97)80007-4
更新日期：1997-01-01 00:00:00
abstract：:Liver diseases affect millions of people worldwide. In most developed countries, the incidence of viral hepatitis is waning as a result of modern advances in disease prevention, diagnosis, and therapies. Expanded programmes for systematic immunisation against hepatitis B virus have also significantly brought down the ...
journal_title：Journal of hepatology
pub_type： 杂志文章,评审
doi：10.1016/j.jhep.2019.03.004
更新日期：2019-07-01 00:00:00
abstract：:Design and endpoints of clinical trials in hepatocellular carcinoma. Llovet JM, Di Bisceglie AM, Bruix J, Kramer BS, Lencioni R, Zhu AX, Sherman M, Schwartz M, Lotze M, Talwalkar J, Gores GJ; for the Panel of Experts in HCC-Design Clinical Trials. The design of clinical trials in hepatocellular carcinoma (HCC) is comp...
journal_title：Journal of hepatology
pub_type： 评论,杂志文章
doi：10.1016/j.jhep.2008.07.012
更新日期：2008-10-01 00:00:00
abstract：:Intracellular ion homeostasis in liver cell is accomplished through the concerted action of ion pumps, carriers and channels. Description of these elements includes the Na+/K+-ATPase, Na+/H+- and Cl-/ HCO3(-)-exchange and Na+-HCO-3(-)- and Na(+)-K(+)-2 Cl(-)-cotransport as well as K+-, Cl(-)-, Na+ and nonselective cat...
journal_title：Journal of hepatology
pub_type： 杂志文章,评审
doi：
更新日期：1996-01-01 00:00:00
abstract：BACKGROUND/AIM:Primary biliary cirrhosis (PBC) is an autoimmune cholestatic liver disease thought to develop through a complex interaction of genetic and environmental factors. It is characterised by T-cell-mediated non-suppurative destructive cholangitis. We have studied the polymorphic cytotoxic T lymphocyte-associat...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(00)80213-5
更新日期：2000-04-01 00:00:00
abstract：BACKGROUND & AIM:Natural killer T (NKT) cells are CD1d-restricted innate-like T cells that modulate innate and adaptive immune responses. Unlike the well-characterized invariant/type I NKT cells, type II NKT cells with a diverse T cell receptor repertoire are poorly understood. This study defines the pathogenic role of...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2017.05.024
更新日期：2017-10-01 00:00:00
abstract：:Portal hypertension is the main cause of complications in patients with chronic liver disease. Over the past 25 years, progress in the understanding of the pathophysiology of portal hypertension was followed by the introduction of an effective pharmacological therapy, consisting mainly of treatments aimed at correctin...
journal_title：Journal of hepatology
pub_type： 杂志文章,评审
doi：10.1016/j.jhep.2010.03.021
更新日期：2010-09-01 00:00:00
abstract：BACKGROUND & AIMS:Several lipid synthesis pathways play important roles in the development and progression of hepatocellular carcinoma (HCC), although the precise molecular mechanisms remain to be elucidated. Here, we show the relationship between HCC progression and alteration of phospholipid composition regulated by ...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2013.02.030
更新日期：2013-08-01 00:00:00
abstract：BACKGROUND/AIM:Smooth muscle antibodies and antinuclear antibodies characterize type 1 autoimmune hepatitis. Our aim was to correlate the appearance and disappearance of these autoantibodies with clinical and histological events. METHODS:One hundred and seven patients were evaluated successively over 128+/-9 months. A...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(99)80096-8
更新日期：1999-03-01 00:00:00
abstract：BACKGROUND & AIMS:IL28B polymorphisms, jaundice, decline in HCV-RNA, IP-10, and gender have been proposed to be indicative of spontaneous clearance of acute hepatitis C virus infection. The aim of this study was to define a score enabling the discrimination of patients with spontaneous clearance of HCV from those with ...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2013.06.028
更新日期：2013-11-01 00:00:00
abstract：:The present study was conducted to evaluate if the increased rate of apoptosis previously reported in the liver of ethanol-treated rats was accompanied by increased cell renewal. A quantitative analysis of apoptosis was performed in rats fed an ethanol-containing liquid diet for 5 weeks. S-phase cells were demonstrate...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(05)80498-2
更新日期：1994-04-01 00:00:00
abstract：:In the human fetal liver, CYP3A7 is expressed as early as the 13th week of gestation. This continues to the perinatal period when it is sharply repressed prior to birth. Concomitantly, the expression of CYP3A4, not detectable in the fetus, sharply increases in the perinatal period to remain elevated throughout adultho...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(97)80497-7
更新日期：1997-01-01 00:00:00
abstract：BACKGROUND/AIMS:The aim of the study was to investigate the effect of a standard albumin load on blood volume distribution, arterial compliance, and the renin-angiotensin-aldosterone system in patients with different degrees of cirrhosis. METHODS:31 patients with cirrhosis (Child classes A/B/C=8/14/9) received an intr...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(03)00160-0
更新日期：2003-07-01 00:00:00
abstract：BACKGROUND/AIMS:The applicability of adult-to-adult living donor liver transplantation has not been established yet. We report the first data in a European center of the process leading to this procedure from the first moment the patients were informed about it. METHODS:In phase 1 of the process, 121 adult patients en...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2005.02.016
更新日期：2005-07-01 00:00:00
abstract：BACKGROUND/AIMS:Treatment of hepatorenal syndrome (HRS) is based on vasoconstrictors. Terlipressin is the one with the soundest evidence. Noradrenalin has been suggested as an effective alternative. The current study was aimed at assessing the efficacy and safety of noradrenalin vs terlipressin in patients with HRS. M...
journal_title：Journal of hepatology
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.jhep.2007.04.010
更新日期：2007-10-01 00:00:00
abstract：BACKGROUND & AIMS:Hepatocellular ballooning is a key finding in nonalcoholic steatohepatitis (NASH). It is conventionally defined by hemotoxylin and eosin (H&E) staining showing enlarged cells with rarefied cytoplasm and recently by changes in the cytoskeleton. Fat droplets are emerging as important organelles in cell ...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2010.04.031
更新日期：2010-10-01 00:00:00
abstract：BACKGROUND & AIMS:Regulatory CD4(+) T cells (Tregs) are considered to affect outcomes of HCV infection, because they increase in number during chronic hepatitis C and can suppress T-cell functions. METHODS:Using microarray analysis, in situ immunofluorescence, ELISA, and flowcytometry, we characterised functional diff...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2013.04.011
更新日期：2013-08-01 00:00:00
abstract：BACKGROUND & AIMS:Hepatitis C virus (HCV) incidence among HIV-positive men who have sex with men (MSM) has increased since 2000, although there are regional differences. We aimed to 1) estimate trends in HCV incidence among HIV-positive MSM, 2) assess the association between incidence and geographical region, age and H...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2017.03.038
更新日期：2017-08-01 00:00:00
abstract：BACKGROUND & AIMS:Robust data on hepatocellular carcinoma (HCC) incidence among HIV/hepatitis B virus (HBV)-coinfected individuals on antiretroviral therapy (ART) are needed to inform HCC screening strategies. We aimed to evaluate the incidence and risk factors of HCC among HIV/HBV-coinfected individuals on tenofovir d...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2019.03.032
更新日期：2019-08-01 00:00:00
abstract：BACKGROUND/AIMS:Central nervous system abnormalities such as fatigue and depression occur more frequently in chronic hepatitis C virus (HCV) infection than in many other causes of chronic liver disease. The finding that fatigue is unrelated to activity of hepatitis or mode of infection could indicate an independent eff...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(02)00172-1
更新日期：2002-09-01 00:00:00
abstract：BACKGROUND & AIMS:Steatohepatitis (SH) is associated with mitochondrial dysfunction and excessive production of superoxide, which can then be converted into H(2)O(2) by SOD2. Since mitochondrial GSH (mGSH) plays a critical role in H(2)O(2) reduction, we explored the interplay between superoxide, H(2)O(2), and mGSH in n...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2012.05.024
更新日期：2012-10-01 00:00:00
abstract：BACKGROUND/AIMS:Liver negative-strand hepatitis C virus (HCV) RNA is the most direct indicator of active viral replication but has only been examined in a few semiquantitative studies. METHODS:Positive- and negative-strand HCV RNA in the right (R) and left (L) liver lobes was quantified by rTth-based strand-specific r...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2005.10.014
更新日期：2006-02-01 00:00:00
abstract：BACKGROUND/AIMS:Leptin has a particular profibrogenic role in the liver. We investigated whether IFN-alpha influences leptin production in patients with chronic hepatitis B (CHB) and C (CHC). Leptin was determined in serial samples from 63 CHB and 42 CHC IFN-alpha treated patients. Furthermore, we evaluated whether lep...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2006.01.025
更新日期：2006-05-01 00:00:00
abstract：BACKGROUND/AIMS:Primary and secondary liver tumours are a common clinical problem, with a poor prognosis in most cases. Surgical resection offers the best outcome, but is only appropriate for the minority. Thermal ablation techniques have been described, but the lack of an optimal means of monitoring has limited their ...
journal_title：Journal of hepatology
pub_type： 临床试验,杂志文章
doi：10.1016/s0168-8278(99)80234-7
更新日期：1999-08-01 00:00:00
abstract：BACKGROUND/AIMS:Hepatocellular carcinoma recurrence after curative treatment adversely influences clinical outcome. It is important to explore adjuvant therapies. This phase II/stage 1 multi-center, randomized trial investigated the safety, optimal dosage and preliminary efficacy of PI-88, a novel heparanase inhibitor,...
journal_title：Journal of hepatology
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1016/j.jhep.2008.12.023
更新日期：2009-05-01 00:00:00
abstract：BACKGROUND/AIMS:The liver is the main source of circulating insulin-like growth factor binding proteins. In man, the cellular origin of insulin-like growth factor binding proteins has remained obscure. METHODS:Human hepatocytes isolated from surgical specimens were purified and cultured using a collagen gel immobiliza...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/0168-8278(95)80201-0
更新日期：1995-10-01 00:00:00
