Abstract：
:While the Vif protein of human immunodeficiency virus type 1 (HIV-1) is essential for viral replication in non-permissive cells, it is rapidly degraded intracellularly. We have previously suggested that the rapid turn-over of Vif is biologically meaningful to prevent detrimental effects of this protein at high expression levels. We now studied the mechanism of Vif degradation by examining the blocking effect of protease inhibitors in pulse/chase experiments and by monitoring the extent of Vif ubiquitination. The rapid turn-over of Vif could be blocked by proteasome inhibitors, and Vif was highly ubiquitinated. Cytoskeletal Vif was found to be more stable than soluble cytosolic Vif. These degradation characteristics of Vif were cell type-independent and observed in both non-permissive and permissive cells. Characterization of a series of vif deletion mutants showed that amino acids predicted to be important for formation of beta-strand structures (amino acid nos. 63-70 and 86-89) were critical for maintaining a normal expression level of Vif and for viral infectivity. Finally, we performed comparative stability analysis of the four HIV-1 accessory proteins. Vif was unique in its short half-life and in the magnitude of the degradation. Taken together, we conclude that the proteasome degradation of HIV-1 Vif is a virologically important process and crucial for the function of Vif.
journal_name
Microbes Infectjournal_title
Microbes and infectionauthors
Fujita M,Akari H,Sakurai A,Yoshida A,Chiba T,Tanaka K,Strebel K,Adachi Adoi
10.1016/j.micinf.2004.04.011subject
Has Abstractpub_date
2004-07-01 00:00:00pages
791-8issue
9eissn
1286-4579issn
1769-714Xpii
S1286-4579(04)00159-5journal_volume
6pub_type
杂志文章abstract：:Helicobacter hepaticus strain 3B1 (H. hepaticus) contains a genomic island of approximately 71 kb, HHGI1, with some of the common features shared among known bacterial pathogenicity islands. In this study, we characterized the pathogenic potential of HHGI1 by infecting B6.129-IL10 tm1Cgn (IL10-/-) mice with an isogeni...
journal_title：Microbes and infection
pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Microbes and infection
pub_type： 杂志文章,评审
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abstract：:Recombinant BCG strain that secretes the chimaeric protein of Ag85B and ESAT-6 has been demonstrated to augment Th1 immune response in C57BL/6 mice. In this paper, we studied the immunostimulatory activity of the recombinant BCG strains in vitro and found out that rBCG-A(N)-E-A(C) activated THP-1 cells and induced hig...
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pub_type： 杂志文章
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journal_title：Microbes and infection
pub_type： 杂志文章
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journal_title：Microbes and infection
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journal_title：Microbes and infection
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pub_type： 杂志文章
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journal_title：Microbes and infection
pub_type： 杂志文章
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更新日期：2006-04-01 00:00:00
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journal_title：Microbes and infection
pub_type： 杂志文章
doi：10.1016/j.micinf.2006.05.008
更新日期：2006-08-01 00:00:00
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journal_title：Microbes and infection
pub_type： 杂志文章
doi：10.1016/j.micinf.2009.07.006
更新日期：2009-11-01 00:00:00
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journal_title：Microbes and infection
pub_type： 杂志文章
doi：10.1016/j.micinf.2011.07.008
更新日期：2011-12-01 00:00:00
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journal_title：Microbes and infection
pub_type： 杂志文章
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更新日期：2018-03-01 00:00:00
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journal_title：Microbes and infection
pub_type： 杂志文章
doi：10.1016/j.micinf.2011.06.002
更新日期：2011-11-01 00:00:00
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journal_title：Microbes and infection
pub_type： 杂志文章,评审
doi：10.1016/s1286-4579(02)01603-9
更新日期：2002-07-01 00:00:00
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journal_title：Microbes and infection
pub_type： 杂志文章
doi：10.1016/j.micinf.2016.09.005
更新日期：2017-01-01 00:00:00
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journal_title：Microbes and infection
pub_type： 杂志文章
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更新日期：2011-11-01 00:00:00
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journal_title：Microbes and infection
pub_type： 杂志文章
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更新日期：2000-07-01 00:00:00
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journal_title：Microbes and infection
pub_type： 杂志文章,评审
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更新日期：2001-06-01 00:00:00
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journal_title：Microbes and infection
pub_type： 杂志文章
doi：10.1016/j.micinf.2011.08.011
更新日期：2012-01-01 00:00:00
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journal_title：Microbes and infection
pub_type： 杂志文章
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更新日期：2005-05-01 00:00:00
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journal_title：Microbes and infection
pub_type： 杂志文章
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更新日期：2015-06-01 00:00:00
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journal_title：Microbes and infection
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更新日期：2016-09-01 00:00:00
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journal_title：Microbes and infection
pub_type： 杂志文章
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更新日期：2010-06-01 00:00:00
abstract：:Onchocerca volvulus is a tissue-invasive parasitic nematode causing skin and eye pathology in human onchocerciasis. The filariae habour abundant intracellular Wolbachia bacteria, now recognised as obligatory symbionts, and therefore emerging as a novel target for chemotherapy. Recent research demonstrates that both th...
journal_title：Microbes and infection
pub_type： 杂志文章,评审
doi：10.1016/j.micinf.2003.11.003
更新日期：2004-01-01 00:00:00
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journal_title：Microbes and infection
pub_type： 杂志文章
doi：10.1016/j.micinf.2008.03.006
更新日期：2008-05-01 00:00:00
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journal_title：Microbes and infection
pub_type： 杂志文章,评审
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更新日期：2000-04-01 00:00:00
abstract：:In this paper we evaluate the role of human γδ T cells in control of Francisella tularensis infection. Using an in vitro model of infection, a reduction in bacterial numbers was detected in the presence of human γδ T cells for both attenuated LVS and virulent SCHU S4 strains of F. tularensis. Antibody neutralisation o...
journal_title：Microbes and infection
pub_type： 杂志文章
doi：10.1016/j.micinf.2012.02.001
更新日期：2012-07-01 00:00:00
abstract：:The differentiation and survival of developing alpha beta thymocytes depends on effective T-cell receptor (TCR) signaling upon recognition of self peptide/major histocompatibility complex (MHC) molecule ligands. Although this concept is uniformly accepted with regard to immature thymocytes, there are conflicting repor...
journal_title：Microbes and infection
pub_type： 杂志文章,评审
doi：10.1016/s1286-4579(02)01571-x
更新日期：2002-04-01 00:00:00
