Three-dimensional organotypic models of human colonic epithelium to study the early stages of enteric salmonellosis.


:In vitro cell culture models used to study how Salmonella initiates disease at the intestinal epithelium would benefit from the recognition that organs and tissues function in a three-dimensional (3-D) environment and that this spatial context is necessary for development of cultures that more realistically resemble in vivo tissues/organs. Our aim was to establish and characterize biologically meaningful 3-D models of human colonic epithelium and apply them to study the early stages of enteric salmonellosis. The human colonic cell line HT-29 was cultured in 3-D and characterized by immunohistochemistry, histology, and scanning electron microscopy. Wild-type Salmonella typhimurium and an isogenic SPI-1 type three secretion system (TTSS) mutant derivative (invA) were used to compare the interactions with 3-D cells and monolayers in adherence/invasion, tissue pathology, and cytokine expression studies. The results showed that 3-D culture enhanced many characteristics normally associated with fully differentiated, functional intestinal epithelia in vivo, including better organization of junctional, extracellular matrix, and brush-border proteins, and highly localized mucin production. Wild-type Salmonella demonstrated increased adherence, but significantly lower invasion for 3-D cells. Interestingly, the SPI-I TTSS mutant showed wild-type ability to invade into the 3-D cells but did not cause significant structural changes to these cells. Moreover, 3-D cells produced less interleukin-8 before and after Salmonella infection. These results suggest that 3-D cultures of human colonic epithelium provide valuable alternative models to study human enteric salmonellosis with potential for novel insight into Salmonella pathogenesis.


Microbes Infect


Microbes and infection


Höner zu Bentrup K,Ramamurthy R,Ott CM,Emami K,Nelman-Gonzalez M,Wilson JW,Richter EG,Goodwin TJ,Alexander JS,Pierson DL,Pellis N,Buchanan KL,Nickerson CA




Has Abstract


2006-06-01 00:00:00














  • Influenza genome diversity and evolution.

    abstract::The influenza viruses contain highly variable genomes and are able to infect a wide range of host species. Large-scale sequencing projects have collected abundant influenza sequence data for assessing influenza genome diversity and evolution. This work reviews current influenza sequence databases characteristics and s...

    journal_title:Microbes and infection

    pub_type: 杂志文章


    authors: Tsai KN,Chen GW

    更新日期:2011-05-01 00:00:00

  • Development of an effective Japanese encephalitis virus-specific DNA vaccine.

    abstract::Intramuscular immunization with DNA vaccines has been shown to induce a broad range of immune responses and protective immunity in many animal models, but it is less effective in primates. One reason for this may be the low expression of vector-encoded antigen in cells. Here we report that the use of vaccine vector (p...

    journal_title:Microbes and infection

    pub_type: 杂志文章


    authors: Wu CJ,Li TL,Huang HW,Tao MH,Chan YL

    更新日期:2006-09-01 00:00:00

  • Intestinal epithelial cells express and secrete the CD43 glycoform that contains core 2 O-glycans.

    abstract::Several glycoforms of CD43 are known to regulate cellular interactions in the immune system. One such glycoform, the CD43 that bears core 2 O-glycans, is also known to be expressed on T lymphocytes and natural killer cells, but only after their activation. Previous studies have also shown that when Caco-2 cells, which...

    journal_title:Microbes and infection

    pub_type: 杂志文章


    authors: Amano J,Morimoto C,Irimura T

    更新日期:2001-07-01 00:00:00

  • Co-infection with two different Campylobacter jejuni strains in a patient with the Guillain-Barré syndrome.

    abstract::Campylobacter jejuni is the predominant cause of antecedent infection in Guillain-Barré syndrome (GBS) or Miller Fisher syndrome (MFS). C. jejuni probably triggers GBS or MFS through molecular mimicry between bacterial sialylated lipo-oligosaccharides (LOS) and gangliosides in peripheral nerve tissue. We investigated ...

    journal_title:Microbes and infection

    pub_type: 杂志文章


    authors: Godschalk PC,Gilbert M,Jacobs BC,Kramers T,Tio-Gillen AP,Ang CW,Van den Braak N,Li J,Verbrugh HA,Van Belkum A,Endtz HP

    更新日期:2006-01-01 00:00:00

  • Interplay between parasite cysteine proteases and the host kinin system modulates microvascular leakage and macrophage infection by promastigotes of the Leishmania donovani complex.

    abstract::Kinins, the vasoactive peptides proteolytically liberated from kininogens, were recently recognized as signals alerting the innate immune system. Here we demonstrate that Leishmania donovani and Leishmania chagasi, two etiological agents of visceral leishmaniasis (VL), activate the kinin system. Intravital microscopy ...

    journal_title:Microbes and infection

    pub_type: 杂志文章


    authors: Svensjö E,Batista PR,Brodskyn CI,Silva R,Lima AP,Schmitz V,Saraiva E,Pesquero JB,Mori MA,Müller-Esterl W,Scharfstein J

    更新日期:2006-01-01 00:00:00

  • Induction of systemic and mucosal immune response and decrease in Streptococcus pneumoniae colonization by nasal inoculation of mice with recombinant lactic acid bacteria expressing pneumococcal surface antigen A.

    abstract::Mucosal epithelia constitute the first barriers to be overcome by pathogens during infection. The induction of protective IgA in this location is important for the prevention of infection and can be achieved through different mucosal immunization strategies. Lactic acid bacteria have been tested in the last few years ...

    journal_title:Microbes and infection

    pub_type: 杂志文章


    authors: Oliveira ML,Arêas AP,Campos IB,Monedero V,Perez-Martínez G,Miyaji EN,Leite LC,Aires KA,Lee Ho P

    更新日期:2006-04-01 00:00:00

  • Evidence for Trypanosoma cruzi in adipose tissue in human chronic Chagas disease.

    abstract::Trypanosoma cruzi the cause of Chagas disease persists in tissues of infected experimental animals and humans. Here we demonstrate the persistence of the parasite in adipose tissue from of three of 10 elderly seropositive patients with chronic chagasic heart disease. Nine control patients had no parasites in the fat. ...

    journal_title:Microbes and infection

    pub_type: 杂志文章


    authors: Ferreira AV,Segatto M,Menezes Z,Macedo AM,Gelape C,de Oliveira Andrade L,Nagajyothi F,Scherer PE,Teixeira MM,Tanowitz HB

    更新日期:2011-11-01 00:00:00

  • Autonomous immunity in mucosal epithelial cells: fortifying the barrier against infection.

    abstract::Mucosal epithelial cells express an autonomous innate immune response that controls the overgrowth of invaded bacteria, mitigates the harmful effects of the bacteria carried within, and does not rely on other external arms of the immune response. Epithelial cell autonomous innate immunity "respects" the social biology...

    journal_title:Microbes and infection

    pub_type: 杂志文章,评审


    authors: Ross KF,Herzberg MC

    更新日期:2016-06-01 00:00:00

  • Inhibitory neuropeptide receptors on macrophages.

    abstract::The immune response, both in innate and adaptive immunity, is controlled at several levels, including signaling from the central nervous system. Neuropeptides released within the lymphoid organs modulate the immune response, either as stimulators or inhibitors. The subject of this review is the description of macropha...

    journal_title:Microbes and infection

    pub_type: 杂志文章,评审


    authors: Ganea D,Delgado M

    更新日期:2001-02-01 00:00:00

  • The role of cGMP signalling in regulating life cycle progression of Plasmodium.

    abstract::The 3'-5'-cyclic guanosine monophosphate (cGMP)-dependent protein kinase (PKG) is the main mediator of cGMP signalling in the malaria parasite. This article reviews the role of PKG in Plasmodium falciparum during gametogenesis and blood stage schizont rupture, as well as the role of the Plasmodium berghei orthologue i...

    journal_title:Microbes and infection

    pub_type: 杂志文章,评审


    authors: Hopp CS,Bowyer PW,Baker DA

    更新日期:2012-08-01 00:00:00

  • Control of organ-specific autoimmunity by immunoregulatory CD4(+)CD25(+) T cells.

    abstract::CD4(+)CD25(+) T cells regulate the activity of autoreactive T cells. Depletion of these cells results in the development of a wide-spectrum of organ-specific autoimmune diseases. In vitro model systems have been developed to study the function of these potent suppressor cells. Following their activation via their T-ce...

    journal_title:Microbes and infection

    pub_type: 杂志文章,评审


    authors: McHugh RS,Shevach EM,Thornton AM

    更新日期:2001-09-01 00:00:00

  • Selection of HLA-B57-associated Gag A146P mutant by HLA-B∗48:01-restricted Gag140-147-specific CTLs in chronically HIV-1-infected Japanese.

    abstract::We previously showed the possibility that Gag A146P, which is an escape mutant from HLA-B∗57-restricted CTLs, was selected by HLA-B∗48:01-restricted Gag138-147(LI10)-specific CTLs in a Japanese cohort in which HLA-B∗57 individuals were not detected. We herein demonstrated Gag140-147(GI8) to be the optimal epitope rath...

    journal_title:Microbes and infection

    pub_type: 杂志文章


    authors: Naruto T,Murakoshi H,Chikata T,Koyanagi M,Kawashima Y,Gatanaga H,Oka S,Takiguchi M

    更新日期:2011-08-01 00:00:00

  • Respiratory nosocomial infections in the medical intensive care unit.

    abstract::Intensive care unit (ICU)-acquired lower respiratory tract infections include acute tracheobronchitis and hospital-acquired and ventilator-associated pneumonia (VAP). Nosocomial pneumonia is the second most common hospital-acquired infection and the leading cause of death in hospital-acquired infections. The mortality...

    journal_title:Microbes and infection

    pub_type: 杂志文章


    authors: Cavalcanti M,Valencia M,Torres A

    更新日期:2005-02-01 00:00:00

  • Evaluation of the protective immune response induced in mice by immunization with Schistosoma mansoni schistosomula tegument (Smteg) in association with CpG-ODN.

    abstract::In schistosomiasis, the current control strategy does not prevent reinfection, therefore, vaccine strategies are essential to combat the Schistosoma mansoni. The efficacy vaccine depends on parasite stage and effective adjuvant. We have recently demonstrated that S. mansoni schistosomula tegument (Smteg) is able to ac...

    journal_title:Microbes and infection

    pub_type: 杂志文章


    authors: Teixeira de Melo T,Araujo JM,Campos de Sena I,Carvalho Alves C,Araujo N,Toscano Fonseca C

    更新日期:2013-01-01 00:00:00

  • Respiratory fungal allergy.

    abstract::Fungal allergy including allergic rhinitis, conjunctivitis, bronchial asthma, and allergic bronchopulmonary mycoses results from exposure to spores. In this review we have dealt with the common allergenic fungi and allergens, immunopathogenesis, diagnostic assays, and the possible control of allergy in the future base...

    journal_title:Microbes and infection

    pub_type: 杂志文章,评审


    authors: Kurup VP,Shen HD,Banerjee B

    更新日期:2000-07-01 00:00:00

  • Mammalian RNA virus-derived small RNA: biogenesis and functional activity.

    abstract::The role of virus-derived small RNAs (vsRNAs) has been identified as an antiviral mechanism in plants, arthropods, and nematodes. Although mammalian DNA viruses have been observed to encode functional miRNAs, whether RNA virus infection generates functional vsRNAs remains under discussion. This article reviews the mos...

    journal_title:Microbes and infection

    pub_type: 杂志文章,评审


    authors: Weng KF,Hsieh PT,Huang HI,Shih SR

    更新日期:2015-08-01 00:00:00

  • Epidemiology and pathogenesis of Vibrio vulnificus.

    abstract::Vibrio vulnificus is capable of causing severe and often fatal infections in susceptible individuals. It causes two distinct disease syndromes, a primary septicemia and necrotizing wound infections. This review discusses the interaction of environmental conditions, host factors, and bacterial virulence determinants th...

    journal_title:Microbes and infection

    pub_type: 杂志文章,评审


    authors: Strom MS,Paranjpye RN

    更新日期:2000-02-01 00:00:00

  • Altered macrophage function is associated with severe Burkholderia pseudomallei infection in a murine model of type 2 diabetes.

    abstract::This study used a murine model of type 2 diabetes (BKS.Cg-Dock7(m) +/+Lepr(db)/J mice) to investigate the inflammatory and cellular mechanisms predisposing to Burkholderia pseudomallei infection and co-morbid diabetes. Homozygous db/db (diabetic) mice developed extreme obesity, dyslipidaemia and glucose intolerance le...

    journal_title:Microbes and infection

    pub_type: 杂志文章


    authors: Hodgson KA,Morris JL,Feterl ML,Govan BL,Ketheesan N

    更新日期:2011-12-01 00:00:00

  • Leishmania parasites: could we consider them as living organisms per se?

    abstract::Over the last 10 years - in Microbes and Infection - the publications dealing with protozoan parasites were mainly providing insights on the pathogenic processes leading to the local or systemic damages in the mammals, these parasitic organisms exploit/subvert as hosts. As a result, many investigators introduced the o...

    journal_title:Microbes and infection

    pub_type: 杂志文章


    authors: Milon G

    更新日期:2008-07-01 00:00:00

  • Inflammatory lesion and parasite load are inversely associated in Leishmania amazonensis infected mice genetically selected according to oral tolerance susceptibility.

    abstract::Two strains of mice selected according to extreme phenotypes of susceptibility and resistance to oral tolerance (TS and TR mice, respectively) were infected with 1 x 10(7) Leishmania amazonensis promastigotes and studied comparatively. TS mice developed a minor pathology while permitting parasite growth with the prese...

    journal_title:Microbes and infection

    pub_type: 杂志文章


    authors: Tavares D,da Conceição Ribeiro R,Carlos da Silva A

    更新日期:2006-04-01 00:00:00

  • Virulence factors of schistosomes.

    abstract::This review considers whether the products of schistosomes in the mammalian host can be considered as virulence factors. These include: the cercarial secretions used in infection, those of the migrating schistosomulum, surface-exposed proteins of adult worms in the portal system and their gut vomitus in the context of...

    journal_title:Microbes and infection

    pub_type: 杂志文章,评审


    authors: Wilson RA

    更新日期:2012-12-01 00:00:00

  • Chlamydia trachomatis genes whose products are related to energy metabolism are expressed differentially in active vs. persistent infection.

    abstract::The Chlamydia trachomatis genome encodes glycolysis and pentose phosphate pathway enzymes, two ATP/ADP exchange proteins, and other energy transduction-related components. We asked if and when chlamydial genes specifying products related to energy transduction are expressed during active vs. persistent infection in in...

    journal_title:Microbes and infection

    pub_type: 杂志文章


    authors: Gérard HC,Freise J,Wang Z,Roberts G,Rudy D,Krauss-Opatz B,Köhler L,Zeidler H,Schumacher HR,Whittum-Hudson JA,Hudson AP

    更新日期:2002-01-01 00:00:00

  • Complete development of Cryptosporidium parvum in rabbit chondrocytes (VELI cells).

    abstract::Cryptosporidium parvum is an intracellular protozoan parasite that causes severe infection in humans and animals. The great difficulties in treating people and animals suffering from cryptosporidiosis have prompted the development of in vitro experimental models. The aim of this study was to demonstrate that C. parvum...

    journal_title:Microbes and infection

    pub_type: 杂志文章


    authors: Lacharme L,Villar V,Rojo-Vazquez FA,Suárez S

    更新日期:2004-05-01 00:00:00

  • Neural pathogenesis of enterovirus 71 infection.

    abstract::Enterovirus 71 (EV71) is a neurotropic pathogen that can cause severe neural diseases and complications on infected patients. Clinical observations showed that EV71-induced immune responses may be associated with virus induced neurogenic pulmonary edema. Here reviewed studies that discovered several host molecules as ...

    journal_title:Microbes and infection

    pub_type: 杂志文章,评审


    authors: Weng KF,Chen LL,Huang PN,Shih SR

    更新日期:2010-07-01 00:00:00

  • TLR3 and TLR7 are involved in expression of IL-23 subunits while TLR3 but not TLR7 is involved in expression of IFN-beta by Theiler's virus-infected RAW264.7 cells.

    abstract::Theiler's murine encephalomyelitis virus (TMEV) infects macrophages and causes demyelinating disease (DD) in certain mouse strains. IL-23 p19/p40 and IFN-beta, which are both expressed by macrophages in response to TMEV, could contribute to or prevent DD. Because TMEV may induce macrophages' cytokines through TLR3 and...

    journal_title:Microbes and infection

    pub_type: 杂志文章


    authors: Al-Salleeh F,Petro TM

    更新日期:2007-09-01 00:00:00

  • The Anopheles gambiae cE5 salivary protein: a sensitive biomarker to evaluate the efficacy of insecticide-treated nets in malaria vector control.

    abstract::Evaluation of vector control is crucial for improving malaria containment and, according to World Health Organization, new complementary indicators would be very valuable. In this study the IgG response to the Anopheles-specific cE5 salivary protein was tested as a tool to evaluate the efficacy of insecticide-treated ...

    journal_title:Microbes and infection

    pub_type: 杂志文章


    authors: Marie A,Ronca R,Poinsignon A,Lombardo F,Drame PM,Cornelie S,Besnard P,Le Mire J,Fiorentino G,Fortes F,Carnevale P,Remoue F,Arcà B

    更新日期:2015-06-01 00:00:00

  • The innate immune response to Aspergillus fumigatus.

    abstract::Despite the development of new treatments, the mortality due to invasive pulmonary aspergillosis remains above 50%, reaching 95% in certain situations. The battle against Aspergillus fumigatus involves several components of the pulmonary innate immune system: cells, mediators, and natural antifungal molecules involved...

    journal_title:Microbes and infection

    pub_type: 杂志文章,评审


    authors: Balloy V,Chignard M

    更新日期:2009-10-01 00:00:00

  • Regulation between survival, persistence, and elimination of intracellular mycobacteria: a nested equilibrium of delicate balances.

    abstract::This review elaborates on recent insights gained on the dynamic aspects of interactions between pathogen and the host intracellular machinery in tuberculosis infection. In addition we will also discuss the utility of adopting a systems biology approach, both for a better understanding of TB biology, and for the develo...

    journal_title:Microbes and infection

    pub_type: 杂志文章,评审


    authors: Kumar D,Rao KV

    更新日期:2011-02-01 00:00:00

  • Amoebal endosymbiont Neochlamydia protects host amoebae against Legionella pneumophila infection by preventing Legionella entry.

    abstract::Acanthamoeba isolated from environmental soil harbors the obligate intracellular symbiont Neochlamydia, which has a critical role in host amoebal defense against Legionella pneumophila infection. Here, by using morphological analysis with confocal laser scanning fluorescence microscopy and transmission electron micros...

    journal_title:Microbes and infection

    pub_type: 杂志文章


    authors: Maita C,Matsushita M,Miyoshi M,Okubo T,Nakamura S,Matsuo J,Takemura M,Miyake M,Nagai H,Yamaguchi H

    更新日期:2018-04-01 00:00:00

  • Antibiotics and Clostridium difficile.

    abstract::Clostridium difficile is now established as a major nosocomial pathogen. C. difficile infection is seen almost exclusively as a complication of antibiotic therapy, and is particularly associated with clindamycin and third-generation cephalosporins. Depletion of the indigenous gut microflora by antibiotic therapy has l...

    journal_title:Microbes and infection

    pub_type: 杂志文章,评审


    authors: Freeman J,Wilcox MH

    更新日期:1999-04-01 00:00:00