Abstract:
:Secreted phospholipase B (PLB1), which contains three enzyme activities in the one protein, is necessary for the initiation of pulmonary infection by Cryptococcus neoformans and for dissemination from the lung via the lymphatics and blood. Adhesion to lung epithelium is the first step in this process, therefore we investigated the role of PLB1 in adhesion to a human lung epithelial cell line, A549, using C. neoformans var. grubii wild-type strain H99, a PLB1 deletion mutant (deltaplb1), and a reconstituted strain (deltaplb1rec). Adhesion of H99 and deltaplb1rec was approximately 69% greater than deltaplb1 at 4 h. Adhesion of deltaplb1 significantly increased after killing by chemicals or heat, and Fourier-transformed analysis by FTIR spectroscopy indicated this was due to changes in capsular and/or cell wall polysaccharides and proteins. Inhibition by specific PLB1 antibodies, or inhibitors of phospholipase B (PLB), but not lysophospholipase (LPL) or lysophospholipase transacylase (LPTA) activities decreased the adhesion of H99 and deltaplb1rec by 33-58%. Growth under conditions of osmotic stress and high glucose concentration increased both PLB secretion and subsequent cryptococcal adhesion. Dose-dependent increases (to 67%) in adhesion of live deltaplb1 were observed in the presence of 0.1-2 mM palmitic acid. We conclude that PLB1 plays a role in the binding of C. neoformans to host lung epithelial cells, possibly due to production of fatty acids from plasma membranes and/or surfactant by PLB activity.
journal_name
Microbes Infectjournal_title
Microbes and infectionauthors
Ganendren R,Carter E,Sorrell T,Widmer F,Wright Ldoi
10.1016/j.micinf.2005.10.018subject
Has Abstractpub_date
2006-04-01 00:00:00pages
1006-15issue
4eissn
1286-4579issn
1769-714Xpii
S1286-4579(05)00398-9journal_volume
8pub_type
杂志文章abstract::Intracellular replication of Cryptosporidium parvum (Apicomplexa) involves the generation of several asexual and sexual forms of the parasite. During the stage conversions, complex mechanisms lead to differential structural and functional properties of the parasite. These require a well tuned gene transcription machin...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2006.04.012
更新日期:2006-07-01 00:00:00
abstract::Matrix metalloproteinases constitute a family of structurally related endopeptidases that are crucial for the normal turnover of the extracellular matrix. Elevated levels of MMP-9 have been demonstrated in synovial fluids of rheumatoid arthritis patients, and a correlation with the severity of the disease has been des...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2006.01.001
更新日期:2006-05-01 00:00:00
abstract::Parasites of the Leishmania genus are the causative agents of a complex disease called leishmaniasis. Many activities of infected cells including their responses to a range of stimuli are modulated by Leishmania parasites. This review will profile some of the parasite molecules that target host cell processes for whic...
journal_title:Microbes and infection
pub_type: 杂志文章,评审
doi:10.1016/j.micinf.2014.07.012
更新日期:2014-09-01 00:00:00
abstract::Recent studies have shown that infection with Porphyromonas gingivalis, a major periodontal pathogen, hastens the progression of non-alcoholic fatty liver disease (NAFLD). However, the intracellular fate of P. gingivalis in hepatocytes remains unknown. Here, using oleic-acid-induced HepG2 cells as an in vitro model fo...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2016.05.004
更新日期:2016-09-01 00:00:00
abstract::Several bacterial pathogens interact with their host through protein secretion effectuated by a type VI secretion system (T6SS). Francisella tularensis is a highly pathogenic intracellular bacterium that causes the disease tularemia. Proteins encoded by the Francisella pathogenicity island (FPI), which constitute a ty...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2016.07.006
更新日期:2016-12-01 00:00:00
abstract::There is increasing evidence that myeloid cells express several receptor families, which include both inhibitory and stimulatory isoforms. The expression of receptor isoforms with similar specificities but opposite functions on the same cell is intriguing. What might be the interplay between these receptors? Some clue...
journal_title:Microbes and infection
pub_type: 杂志文章,评审
doi:10.1016/s1286-4579(00)00294-x
更新日期:2000-03-01 00:00:00
abstract::The bacterium Haemophilus ducreyi causes the sexually transmitted disease chancroid, which is characterized by the appearance of mucocutaneous, persistent ulcers on the external genitals. To identify carbohydrate receptors that mediate the attachment of this pathogen to host cells, we investigated the binding of 35S-m...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2006.05.009
更新日期:2006-08-01 00:00:00
abstract::Cytokines are increasingly recognized as important components of the cellular immune responses to intracellular pathogens. In this study, we analyzed the production of TGF-beta, IL-10 and IFN-gamma by PBMC of unexposed naïve subjects and LCL patients after stimulation with live Leishmania guyanensis (L.g.). We demonst...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2007.04.009
更新日期:2007-07-01 00:00:00
abstract::The differential recognition of fungal cell wall polysaccharides that program innate and adaptive immunity to the human opportunistic fungal pathogen Aspergillus fumigatus has been a focus of considerable interest. In a mouse model of fungal conidia aspiration, decreased relative levels of cell wall core carbohydrates...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2017.05.001
更新日期:2017-01-01 00:00:00
abstract::Campylobacter jejuni is the predominant cause of antecedent infection in Guillain-Barré syndrome (GBS) or Miller Fisher syndrome (MFS). C. jejuni probably triggers GBS or MFS through molecular mimicry between bacterial sialylated lipo-oligosaccharides (LOS) and gangliosides in peripheral nerve tissue. We investigated ...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2005.06.022
更新日期:2006-01-01 00:00:00
abstract::Respiratory syncytial virus (RSV) infection causes severe lower respiratory diseases in infancy, early childhood and the elderly. RSV infections respond poorly to current therapies. Therefore, we initiated a search for novel drug targets by investigating the characteristics and identity of RSV adhesion receptors on ma...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/s1286-4579(02)00079-5
更新日期:2003-02-01 00:00:00
abstract::Antineutrophil cytoplasmic antibodies (ANCA) directed against bactericidal/permeability-increasing protein (BPI) were repeatedly found in cystic fibrosis (CF) patients. We analyzed the effect of BPI-ANCA in inhibiting neutrophil-mediated killing of Pseudomonas aeruginosa. The bactericidal effect expressed as percentag...
journal_title:Microbes and infection
pub_type: 信件
doi:10.1016/s1286-4579(02)00049-7
更新日期:2003-01-01 00:00:00
abstract::Evaluation of vector control is crucial for improving malaria containment and, according to World Health Organization, new complementary indicators would be very valuable. In this study the IgG response to the Anopheles-specific cE5 salivary protein was tested as a tool to evaluate the efficacy of insecticide-treated ...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2015.01.002
更新日期:2015-06-01 00:00:00
abstract::Erythritol is a four-carbon sugar preferentially utilized by Brucella spp. The presence of erythritol in the placentas of goats, cows, and pigs has been used to explain the localization of Brucella to these sites and the subsequent accumulation of large amounts of bacteria, eventually leading to abortion. Here we show...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2013.02.002
更新日期:2013-06-01 00:00:00
abstract::The innate immune system detects the presence of microbes through different families of pattern-recognition receptors (PRRs). PRRs detect pathogens of all origins and trigger signaling events that activate innate and adaptive immunity. These events need to be tightly regulated in order to ensure optimal activation whe...
journal_title:Microbes and infection
pub_type: 杂志文章,评审
doi:10.1016/j.micinf.2017.01.003
更新日期:2017-01-01 00:00:00
abstract::Mycoplasma arthritidis causes autoimmune arthritis in rodents. It produces a superantigen (MAM) that simultaneously activates antigen presenting cells and T cells inducing nitric oxide and cytokine release. Nitric oxide is a key inducer and regulator of the immune system activation. Here, we investigated nitric oxide ...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2008.09.010
更新日期:2008-11-01 00:00:00
abstract::The immune response, both in innate and adaptive immunity, is controlled at several levels, including signaling from the central nervous system. Neuropeptides released within the lymphoid organs modulate the immune response, either as stimulators or inhibitors. The subject of this review is the description of macropha...
journal_title:Microbes and infection
pub_type: 杂志文章,评审
doi:10.1016/s1286-4579(00)01361-7
更新日期:2001-02-01 00:00:00
abstract::Pseudomonas aeruginosa, a significant cause of human morbidity and mortality, uses a type 3 secretion system (T3SS) to inject effector toxins into host cells. We previously reported that P. aeruginosa uses ADP-ribosyltransferase (ADPr) activity of the T3SS effector ExoS for intracellular replication. T3SS translocon (...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2012.08.007
更新日期:2012-12-01 00:00:00
abstract::Genome sequences of 36 Rickettsia and Orientia were analyzed using Formal Order Analysis (FOA). This approach takes into account arrangement of nucleotides in each sequence. A numerical characteristic, the average distance (remoteness) - "g" was used to compare of genomes. Our results corroborated previous separation ...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2015.09.012
更新日期:2015-11-01 00:00:00
abstract::The role of virus-derived small RNAs (vsRNAs) has been identified as an antiviral mechanism in plants, arthropods, and nematodes. Although mammalian DNA viruses have been observed to encode functional miRNAs, whether RNA virus infection generates functional vsRNAs remains under discussion. This article reviews the mos...
journal_title:Microbes and infection
pub_type: 杂志文章,评审
doi:10.1016/j.micinf.2015.04.005
更新日期:2015-08-01 00:00:00
abstract::Onchocerca volvulus is a tissue-invasive parasitic nematode causing skin and eye pathology in human onchocerciasis. The filariae habour abundant intracellular Wolbachia bacteria, now recognised as obligatory symbionts, and therefore emerging as a novel target for chemotherapy. Recent research demonstrates that both th...
journal_title:Microbes and infection
pub_type: 杂志文章,评审
doi:10.1016/j.micinf.2003.11.003
更新日期:2004-01-01 00:00:00
abstract::Leprosy is a chronic infection of the skin and nerves caused by Mycobacterium leprae. Erythema nodosum leprosum (ENL) is a reactive state in lepromatous leprosy. Thalidomide has been used to treat ENL since the 1960s. One of its mechanisms of action is anti-inflammatory through selective inhibition of the pro-inflamma...
journal_title:Microbes and infection
pub_type: 杂志文章,评审
doi:10.1016/s1286-4579(02)01645-3
更新日期:2002-09-01 00:00:00
abstract::Human histocompatibility leukocyte antigen (HLA)-G is an antigen-presenting molecule. This review discusses the possibility that this might not be its primary function. HLA-G indeed modulates innate immunity by interacting with immunoglobulin-like receptors and by regulating HLA-E expression and its subsequent interac...
journal_title:Microbes and infection
pub_type: 杂志文章,评审
doi:10.1016/s1286-4579(01)01386-7
更新日期:2001-04-01 00:00:00
abstract::Afipia felis is a Gram-negative alpha-proteobacterium, a rare cause of human cat scratch disease (CSD), and likely a pathogen of amoeba. Here, we show that various members of the genus Afipia attach to and are taken up by various non-professional phagocytic mammalian cells (epithelial CHO, endothelial EA.hy926, epithe...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2006.12.007
更新日期:2007-03-01 00:00:00
abstract::Lipoarabinomannans (LAMs) from mycobacteria were investigated concerning their effect on human neutrophils. Two types of LAM, the mannose-capped ManLAM from the virulent Mycobacterium tuberculosis H37Rv and the mannose-lacking AraLAM from a rapidly growing mycobacterial strain were used. Neither AraLAM nor ManLAM indu...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/s1286-4579(01)01470-8
更新日期:2001-11-01 00:00:00
abstract::The inner ear has been regarded as an immunoprivileged site because of isolation by the blood-labyrinthine barrier. Several reports have indicated the existence of immune cells in the inner ear, but there are no reports showing immunocompetence of the cochlear tissue. In this report, we examined the potential involvem...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2013.04.008
更新日期:2013-07-01 00:00:00
abstract::Infection of mice with Plasmodium berghei NK65 represents a well-recognized malaria model in which infection is accompanied by an intense hepatic inflammatory response. Enzyme-inducible nitric oxide synthase is an important regulator of inflammation and leukocyte recruitment in microvessels, but these functions have y...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2013.08.001
更新日期:2013-11-01 00:00:00
abstract::CCR5-tropic (R5) immunodeficiency virus type 1 (HIV-1) strains are highly transmissible during the early stage of infection in humans, whereas CXCR4-tropic (X4) strains are less transmissible. This study aimed to explore the basis for early phase R5 and X4 HIV-1 infection in vivo by using humanized mice dually challen...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2015.02.002
更新日期:2015-05-01 00:00:00
abstract::Interleukin (IL)-15 is a recently described cytokine that resembles IL-2 in its biological activities, stimulating natural killer cells, T cells and B cells to proliferate, secrete cytokines, and exhibit increased cytotoxicities or produce antibody. IL-15 also exerts unique functions such as stimulation of phagocytes,...
journal_title:Microbes and infection
pub_type: 杂志文章,评审
doi:10.1016/s1286-4579(00)00329-4
更新日期:2000-04-01 00:00:00
abstract::While the Vif protein of human immunodeficiency virus type 1 (HIV-1) is essential for viral replication in non-permissive cells, it is rapidly degraded intracellularly. We have previously suggested that the rapid turn-over of Vif is biologically meaningful to prevent detrimental effects of this protein at high express...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2004.04.011
更新日期:2004-07-01 00:00:00