Preservation of human saphenous vein against reactive oxygen species-induced oxidative stress by green tea polyphenol pretreatment.

Abstract:

:The injurious effects of reactive oxygen species on venous tissues and the potential protective role played by green tea polyphenol (GTPP) on human saphenous veins were investigated. Oxidative stress was induced exogenously in the vein segments, either by adding 0.8 or 1.6 M of H2O2, or by using 80 or 160 U/L of xanthine oxidase in the presence of xanthine (0.5 mM). After incubation, the viability of the endothelial cells dissociated from veins and the histology of the veins were evaluated. Due to both types of treatment, a significant decrease in cellular viability, severe morphological changes in the veins, and extracellular structural damage were induced. The H2O2-induced alterations were prevented by preincubating the veins with either 0.5 or 1.0 mg/ml of GTPP for 1 h. When the oxidative stress was induced by xanthine oxidase, cellular viability and venous structure were preserved at the same polyphenol concentrations. These results demonstrate that GTPP can act as a biological antioxidant and protect veins from oxidative stress-induced toxicity.

journal_name

Artif Organs

journal_title

Artificial organs

authors

Han DW,Suh H,Park YH,Cho BK,Hyon SH,Park JC

doi

10.1111/j.1525-1594.2003.07163.x

subject

Has Abstract

pub_date

2003-12-01 00:00:00

pages

1137-42

issue

12

eissn

0160-564X

issn

1525-1594

pii

7163

journal_volume

27

pub_type

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