Technical aspects on middle molecules: separation, isolation, and identification.

Abstract:

:For the last few years we have attempted to separate and isolate from biological fluids the uremic Middle Molecules (MM). The first step in the treatment of plasma is an ultrafiltration through AN69 membrane. The samples are fractionated by Sephadex G-15 chromatography into 9 peaks "a" to "i' under precise conditions that have been selected in order to exhibit different patterns in the MM range (peak b and c) between uremic and uremic polyneuropathic patients. Further separation of peak b is performed by ion exchange chromatography with Sephadex DEAE A-25 into 7 sub-peaks b; sub-peak b 4-2 is the only one to correlate with neuropathy. Prior to b 4-2 identification studies, specific large scale isolation procedures are necessary. The purity of the product is monitored at each step by thin-layer chromatography on silica gel.

journal_name

Artif Organs

journal_title

Artificial organs

authors

Cueille G,Man NK,Sausse A,Farges JP,Funck-Brentano JL

subject

Has Abstract

pub_date

1981-01-01 00:00:00

pages

8-12

eissn

0160-564X

issn

1525-1594

journal_volume

4 Suppl

pub_type

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