Abstract:
OBJECTIVE:To express the hCGbeta-C3d3 fusion protein in a CHO cell continual expression system to investigate further the adjuvant effects of C3d on contraceptive vaccination. METHOD:We constructed a plasmid pcDNA3-hCGbeta-C3d3 which contains three copies of murine C3d cDNA and the hCGbeta gene by cloning the chimerical hCGbeta-C3d3 cDNA into the eukaryotic vector pcDNA3 downstream of the CMV promoter. The plasmid was transfected into a COS-7 cell transient expression system and a CHO cell continual expression system. RIA was used to detect hCGbeta in the culture supernatant. Western blot and Raji cell immunohistochemical assays were performed to evaluate the expressed protein. Then, 6-8-week-old female BALB/c mice were inoculated intramuscularly with pcDNA3-hCGbeta and pcDNA3-hCGbeta-C3d3, and ELISA was used to assess anti-hCGbeta IgG antibody in serum. RESULTS:In 72 h after COS-7 cells were transfected with the plasmid pcDNA3-hCGbeta-C3d3, 1.0x10(5) cells could secrete 152 ng of the recombinant protein (calculated by hCGbeta contained). The transfected CHO cells, which were then screened by G418, could continuously secrete the fusion protein at 660 ng/10(6) cells/48 h. The hCGbeta-C3d3 protein was purified by anti-hCGbeta immunoaffinity chromatography. Raji cell immunohistochemical assay demonstrated that both the hCGbeta and C3d3 were successfully fused. After DNA immunization intramuscularly, the anti-hCGbeta IgG antibody titer in the pcDNA3-hCGbeta-C3d3 immunized group was 243-fold higher than that of the pcDNA3-hCGbeta immunized group. CONCLUSION:We have expressed the hCGbeta-C3d3 protein successfully, both in a transient expression system (COS-7 cells) and in a stable expression system (CHO cells). The C3d3 molecular adjuvant can enhance significantly the immunogenecity of hCGbeta antigen in DNA immunization.
journal_name
J Reprod Immunoljournal_title
Journal of reproductive immunologyauthors
Li DJ,Wang HM,Li L,Zhao XR,Wang MY,Zhu Y,Meng Y,Yuan MMdoi
10.1016/j.jri.2003.09.001subject
Has Abstractpub_date
2003-12-01 00:00:00pages
129-41issue
2eissn
0165-0378issn
1872-7603pii
S016503780300113Xjournal_volume
60pub_type
杂志文章abstract::The objective of these experiments was to determine the effects of neonatal thymectomy on the induction of experimental autoimmune orchitis in inbred rats of the Fischer 344 and Lewis strains. It was found that thymectomy alone in Lewis rats, and thymectomy followed by immunization with testicular extract in both Lewi...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(79)90001-9
更新日期:1979-12-01 00:00:00
abstract::The main message of this work is the fact that female sex hormones, progesterone and estradiol, whose levels significantly rise during pregnancy, inhibit the production of anti-inflammatory cytokine IL-10 with no apparent effect on pro-inflammatory cytokine TNF-α by activated MZ B cells. This is an important piece of ...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2016.05.008
更新日期:2016-08-01 00:00:00
abstract::Placental hypoxia following the immature remodeling of spiral arteries by extravillous cytotrophoblasts (CTs) is focused on the pathogenesis of pre-eclampsia. At the same time, the expression of human leukocyte antigen (HLA)-G is decreased at the protein and mRNA levels in the pre-eclamptic placenta. In view of the po...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2004.07.001
更新日期:2004-10-01 00:00:00
abstract::Cytokines present in the reproductive system play an important role both in the modulation of immune responses to infectious challenge and in the establishment and maintenance of pregnancy. Interleukin 18 (IL-18) has been regarded as an important regulator of innate and acquired immune response, but its expression and...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2011.07.002
更新日期:2011-12-01 00:00:00
abstract::There is now evidence that the classical HLA class I molecule HLA-C is expressed on extravillous trophoblasts together with non-classical HLA-G molecules. Since clones of NK-cells are inhibited differently by supertypic epitopes associated with HLA-C and -B alleles we found it of interest to study HLA-C and -Bw polymo...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/s0165-0378(97)00073-9
更新日期:1997-12-15 00:00:00
abstract::Few studies have assessed longitudinal changes in circulating cytokine levels during normal pregnancy. We have examined the natural history of maternal plasma cytokines from early- to mid-pregnancy in a large, longitudinal cohort. Multiplex flow cytometry was used to measure interleukin (IL)-2, IL-6, IL-12, tumor necr...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2007.06.051
更新日期:2008-04-01 00:00:00
abstract::The expression of the nonclassical MHC class Ib molecule HLA-G is nearly exclusively restricted to the feto-maternal interface during pregnancy. There it probably serves the same physiological functions already known for classical MHC class I molecules; these include peptide presentation, natural killer cell (NK) inhi...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/s0165-0378(99)00029-7
更新日期:1999-07-01 00:00:00
abstract::Peripheral blood levels of K cells were measured in normal pregnant and post-partum women by a plaque-forming cell technique that detects K cells on the basis of their activity of antibody-dependent cell-mediated cytotoxicity (ADCC). Peripheral K cells decreased throughout normal pregnancy (n = 46; 7.8 +/- 3.4%, P les...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(89)90042-9
更新日期:1989-03-01 00:00:00
abstract::A quantitative immunohistochemical study of IgA-containing plasma cells in the human uterine cervix has shown that the number of such cells increases during the late secretory phase of the menstrual cycle but decreases in the early secretory phase of the cycle, in pregnancy, in cystic glandular hyperplasia of the endo...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(82)90020-1
更新日期:1982-02-01 00:00:00
abstract::Visfatin (PBEF/Nampt) is an adipocytokine that exerts pleiotropic effects within the human body, particularly affecting its metabolism and immunity. Visfatin was originally identified as being secreted by peripheral blood lymphocytes acting as a pre-B-cell colony-enhancing factor (PBEF). However, it was subsequently r...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章,评审
doi:10.1016/j.jri.2015.09.004
更新日期:2015-11-01 00:00:00
abstract::The feasibility of producing epitope-specific antigens by mutation of the gene is demonstrated, the aim being to eliminate unwanted surface epitopes yet allowing the natural folding of the protein to maintain the desired epitope(s). The model protein is the beta subunit of human chorionic gonadotropin (hCG beta) which...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(96)00978-3
更新日期:1996-08-01 00:00:00
abstract::Although antisperm autoantibody responses to obstruction of the male reproductive system have been documented, information on the nature of the cognate sperm autoantigens has been limited. In the present study, the patterns of sperm autoantigens recognized by sera from rats after obstruction of the vas deferens or epi...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/s0165-0378(98)00090-4
更新日期:1999-05-01 00:00:00
abstract::Anticardiolipin antibodies (aCL) were eluted from the placentae of four women with elevated serum levels of aCL, demonstrating that these antibodies are bound to affected placentae. Anticardiolipin antibodies bound to affected placentae were only of the IgG isotype and the level of aCL in placental eluates did not ref...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(93)90064-o
更新日期:1993-12-01 00:00:00
abstract::The rabbit sperm membrane autoantigen, RSA-1, has been isolated from the kidneys of bilaterally vasectomized rabbits. Using detergent extraction the IgG-RSA-1 complex in the kidney was isolated first on an anti-IgG immunocolumn. The eluted IgG-RSA-1 complex was then applied to an anti-sperm autoantibody column which b...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(84)90006-8
更新日期:1984-02-01 00:00:00
abstract::Using a passive haemagglutination assay, agglutinins against trophoblast components were detected in term maternal sera at titres above those of non-pregnant control sera. These agglutinin titres were independent of the family relationship between individual sera and trophoblast samples. They were unaffected by adsorp...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(86)90031-8
更新日期:1986-12-01 00:00:00
abstract::Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a classical haematopoietic cytokine which has also been implicated in placental growth and development. In this study we have performed a detailed immunohistological localization of the low affinity GM-CSF receptor (GM-CSF-R alpha) in human first trimester i...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(94)90037-x
更新日期:1994-03-01 00:00:00
abstract::We sought to determine whether cytokine expression in peripheral blood mononuclear cells is altered in patients with preeclampsia and in patients with a history of recurrent spontaneous abortion (RSA). Twenty-four patients with preeclampsia and twenty patients with a history of RSA were included into the study. Two co...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/s0165-0378(01)00128-0
更新日期:2002-03-01 00:00:00
abstract::Endometriosis is a chronic benign disease that affects women of reproductive age causing abdominal pain and infertility. Its pathogenesis remains obscure despite all the research conducted over the past 100 years. However, there is a consensus among the specialists that the basis of its pathophysiology would be multif...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章,评审
doi:10.1016/j.jri.2013.03.003
更新日期:2013-06-01 00:00:00
abstract::Human pre-implantation stage embryos cultured in vitro spontaneously secreted a factor capable of inducing histamine-release from human blood basophils. The embryo-derived histamine-releasing factor (EHRF) has been isolated from the culture medium by means of heparin-Sepharose affinity chromatography. The factor bound...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(88)90047-2
更新日期:1988-06-01 00:00:00
abstract::The immune reactivity of lymphoid cells from pregnant mice was studied during the course of pregnancy in primiparous and multiparous animals either " isopregnant " (male and female of same strain) or " allopregnant " (male and female differing at H-2), using a local GVH assay (CBA lymphoid cells injected into (CBA X A...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(84)90024-x
更新日期:1984-05-01 00:00:00
abstract:OBJECTIVE:Repeated implantation failure (RIF) has been shown related to maternal immune imbalance. Many studies suggested that prednisone promoted the Th17/Treg balance shift to the direction of immune tolerance. Our study aimed to evaluate the role of prednisone in Th17/Treg balance and pregnancy outcome in RIF patien...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2020.103245
更新日期:2021-02-01 00:00:00
abstract::Currently, different approaches are used to select oocytes for in vitro fertilization (IVF) procedures, but they do not assure a significant association with the pregnancy outcome. Since several studies have proposed the expression of HLA-G antigens in early embryos to be a possible marker of elevated implantation rat...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2007.02.005
更新日期:2007-06-01 00:00:00
abstract::Various ELISA methods have been applied by different research centers to test the efficiency of this approach for the diagnosis of sperm-immune infertility cases. The antigens used were either whole spermatozoa or solubilized spermatozoal membrane preparations and were immobilized on microtiter plates, except in one c...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(85)90004-x
更新日期:1985-12-01 00:00:00
abstract:BACKGROUND:Elongation factor Tu GTP binding domain containing 2 (EFTUD2) is an alternative splicing factor that modulates cell differentiation and activation processes. EFTUD2 is known to modulate immune responses and mutation of the EFTUD2-gene lead to fetal malformation. Little is known about its expression and role ...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2020.103149
更新日期:2020-08-01 00:00:00
abstract::Miscarriages in patients with antiphospholipid (aPL) antibodies have been attributed to thrombosis of placental vessels. However, we have shown that inflammation plays a crucial role in fetal injury. We identified tissue factor (TF), the major cellular activator of the coagulation cascade, as a key mediator in inflamm...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2009.03.003
更新日期:2009-11-01 00:00:00
abstract:INTRODUCTION:The aim of this study was to compare serum levels of leptin in women with unexplained infertility with fertile subjects. MATERIAL AND METHOD:Serum leptin levels of 27 infertile and 30 fertile women on day 3 of the menstrual cycle were assessed and compared in this prospective age and body mass index (BMI)...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2007.04.001
更新日期:2007-10-01 00:00:00
abstract::Members of the galectin family have been shown to exert several roles in the context of reproduction. They contribute to placentation, maternal immune regulation and facilitate angiogenesis encompassing decidualisation and placenta formation during pregnancy. In the context of neo-vascularisation, galectins have been ...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章,评审
doi:10.1016/j.jri.2014.12.001
更新日期:2015-04-01 00:00:00
abstract::Viviparity has many evolutionary advantages but brings with it the problem of the semi-allogeneic foetus having to coexist with the mother for the duration of pregnancy. In species with haemochorial placentation this problem is particularly evident as foetal trophoblast cells are extensively intermingled with maternal...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章,评审
doi:10.1016/j.jri.2012.10.006
更新日期:2013-03-01 00:00:00
abstract::Pre-eclampsia (PE) remains the leading cause of pregnancy-associated mortality and morbidity, urging the need for a better understanding of its aetiology and pathophysiological progression. A key characteristic of PE is a systemic, exaggerated, inflammatory condition involving abnormal cytokine levels in serum, altere...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2014.02.004
更新日期:2014-06-01 00:00:00
abstract::Interferon-gamma (IFN-gamma) is a lymphokine, produced by activated T lymphocytes, which plays a key regulatory role in the host immunological responses. In addition, IFN-gamma is expressed by human and porcine trophoblast. As IFN-gamma exerts its biological functions through specific cell surface receptors and a grea...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(94)90013-2
更新日期:1994-08-01 00:00:00