Abstract:
:Human pre-implantation stage embryos cultured in vitro spontaneously secreted a factor capable of inducing histamine-release from human blood basophils. The embryo-derived histamine-releasing factor (EHRF) has been isolated from the culture medium by means of heparin-Sepharose affinity chromatography. The factor bound to the column and was then eluted by increasing the buffer molarity to 1.5 M NaCl. EHRF was detected using an enzymatic-isotopic microassay and sensitized basophils known to undergo release with anti-IgE. The EHRF-induced histamine-release was calcium and temperature dependent and the relatively slow kinetics (10 min) were similar to those obtained with anti-IgE. EHRF caused the release of a substantial amount of histamine (48%, n = 18) in a dose-dependent manner. The equivalent fraction isolated from medium containing unfertilized oocytes gave less than 10% of histamine-release using the same source of basophils, suggesting that EHRF was secreted after fertilization. EHRF was very stable since it was resistant to boiling, lyophilization, and to several freeze and thaw treatments. The histamine-releasing activity induced by EHRF was measured in vitro also by means of purified leukocytes containing sensitized basophils. EHRF could represent a message sent by the embryo to the mother to induce histamine release at the time of implantation.
journal_name
J Reprod Immunoljournal_title
Journal of reproductive immunologyauthors
Cocchiara R,Di Trapani G,Azzolina A,Albeggiani G,Geraci Ddoi
10.1016/0165-0378(88)90047-2subject
Has Abstractpub_date
1988-06-01 00:00:00pages
41-52issue
1eissn
0165-0378issn
1872-7603pii
0165-0378(88)90047-2journal_volume
13pub_type
杂志文章abstract::The rosette inhibition test for the detection of early pregnancy factor is described in detail. The extended methodology presented here represents the cumulative experience of three independent laboratories. Special reference is made to the effect on the assay of varying the conditions of rosette formation between lym...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(82)90001-8
更新日期:1982-09-01 00:00:00
abstract::63 female rhesus monkeys were actively immunized against the beta-subunit of ovine luteinizing hormone (oLH beta). 75% of these monkeys developed antibodies which were sufficiently high to induce infertility. We report here the in vivo neutralizing potencies of anti-oLH beta antisera from 14 monkeys and the correlatio...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(83)90235-8
更新日期:1983-07-01 00:00:00
abstract::HLA-G is a non-classical MHC class 1 molecule, expressed primarily on human foetal trophoblast cells, which exhibits almost no genetic polymorphism. Because of these unusual features, HLA-G has been suggested to help prevent maternal immune attack of the semi-allogeneic foetus. The aim of these experiments was to inve...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/s0165-0378(00)00070-x
更新日期:2000-08-01 00:00:00
abstract::A successful pregnancy is characterised by an increase in Th2 cytokines and suppression of Th1 cytokine production. A Th1 to Th2 cytokine shift is also observed in the disease progression of HIV infection. Highly active antiretroviral therapy (HAART) suppresses HIV viremia, increases CD4+ cell counts and counteracts t...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2005.12.001
更新日期:2006-06-01 00:00:00
abstract::The tissue distribution, molecular weight, and biochemical nature of an antigen detected by a mouse monoclonal antibody designated 283D3 and raised against rabbit visceral yolk sac endodermal cells, has been investigated. The antigen is located on the luminal side of apical tubules and large sub-apical vesicles in rab...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(93)90046-k
更新日期:1993-04-01 00:00:00
abstract::The state of pregnancy is an immunological enigma during which the body must prevent rejection of the antigenically foreign fetus while at the same time maintain sufficient maternal host defense mechanisms to combat infection. Although most studies on the immunology of pregnancy focus on immune suppression, several st...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/s0165-0378(98)00076-x
更新日期:1998-11-01 00:00:00
abstract::Decidual stromal cells (DSCs) are an important component of decidual tissues where they are in strict proximity with immune cells. Although previous research has indicated that DSCs participate in the regulation of immune cells during pregnancy, the crosstalk between DSCs and decidual NK cells (dNKs) has not been full...
journal_title:Journal of reproductive immunology
pub_type: 临床试验,杂志文章
doi:10.1016/j.jri.2015.01.004
更新日期:2015-06-01 00:00:00
abstract::There is conflicting evidence for the deposition of complement in spiral arteries in normal and abnormal human pregnancies. The immunogold silver staining (IGSS) technique was used to investigate the distribution of C1q, C3d, C4, C6 and C9 within the spiral arteries of formalin-fixed normal pregnancy hysterectomy spec...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(87)90040-4
更新日期:1987-10-01 00:00:00
abstract::The mixed lymphocyte culture (MLC) of maternal and newborn (cord) cells is significantly weaker than that of father-newborn and control-newborn cultures. This hyporeactivity was found not to be due to an impaired function or tolerance of either the maternal or neonatal cells. We investigated the possibility that a spe...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(84)90041-x
更新日期:1984-01-01 00:00:00
abstract::Several kinds of circulating autoantibodies and peripheral blood lymphocyte subsets were studied in 20 patients with secondary amenorrhea manifesting hormonal and clinical features of premature ovarian failure (POF). More than one kind of autoantibody was detected in 14 patients (70%). Seven patients (35%) had anti-th...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(87)90021-0
更新日期:1987-11-01 00:00:00
abstract::The sequence of biochemical and molecular events associated with decidualization in the primate remain unclear. In the baboon, the sequential changes during this period in vivo are characterized by the downregulation of alpha-smooth actin followed by induction of cyclooxygenase-2 (COX-2) at the implantation site and t...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章,评审
doi:10.1016/s0165-0378(01)00141-3
更新日期:2002-05-01 00:00:00
abstract::Hypertensive disorders of pregnancy (HDP) represent 10% of human births globally and the major complication preeclampsia has a 3-5% prevalence. The etiology of HDP remains uncertain; however, major advances have been made over the last 25 years. The Seventh International Workshop on Reproductive Immunology, Immunologi...
journal_title:Journal of reproductive immunology
pub_type: 历史文章,杂志文章,评审
doi:10.1016/j.jri.2011.02.003
更新日期:2011-05-01 00:00:00
abstract::Mammalian pregnancy is a complex phenomenon allowing the maternal immune system to support its allogeneic fetus. Physiological pathways protecting the fetus from rejection are thought to be comparable with those leading to allograft acceptance. Heme oxygenase (HO)-1 is known to protect locally against rejection in tra...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2005.10.001
更新日期:2006-02-01 00:00:00
abstract::Antiphospholipid antibodies (aPL) are a family of autoantibodies that are associated with pregnancy complications including stillbirth and recurrent miscarriage. Infertile women, and those with recurrent IVF implantation failure, have an increased incidence of aPL (22% and 30%, respectively) compared with a healthy, f...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2008.11.005
更新日期:2009-06-01 00:00:00
abstract::The present investigation was undertaken to study the association between placental apoptosis and pre-eclampsia, discriminating between pre-eclamptic pregnancies with appropriate-, and small-for-gestational-age (SGA), infants. Twenty pregnancies with pre-eclampsia and SGA (birth weight at or below -2 standard deviatio...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2003.10.001
更新日期:2004-02-01 00:00:00
abstract::Previous studies in our laboratory indicated that immunization of male and female Wistar and Lewis rats with epididymal protein DE, resulted in the development of anti-DE antibodies in over 90% of the animals, with a significant and reversible reduction of fertility. In the present study, ELISA assays performed to ana...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(95)00927-d
更新日期:1995-05-01 00:00:00
abstract::In our earlier study, we have reported the identification and characterization of a 57-kDa sperm membrane protein from human spermatozoa. The protein was found to be localized on the acrosome in acrosome intact spermatozoa and shifted to equatorial region after acrosomal induction. Further, we demonstrated that it pla...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2005.04.004
更新日期:2005-08-01 00:00:00
abstract::Effector cells associated with an aborting fetus appear to be both thymus derived (T) and natural killer (NK) cells. In order to test the hypothesis that NK cells are a major effector mediating early spontaneous abortion (less than day 8-10), CBA female mice mated by DBA/2 males were treated with either polyinosinic/c...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(87)90018-0
更新日期:1987-06-01 00:00:00
abstract::Vitamin D is essential for the health of both mother and fetus during pregnancy. In the nonpregnant state, vitamin D demonstrates anti-inflammatory effects, but little is known about this relationship during pregnancy. African-American women are at a higher risk of vitamin D deficiency and for altered inflammatory res...
journal_title:Journal of reproductive immunology
pub_type: 临床试验,杂志文章
doi:10.1016/j.jri.2014.09.054
更新日期:2015-02-01 00:00:00
abstract::Pregnancy is thought to be a state of immunological tolerance. The mechanisms underlying this phenomenon are still poorly understood. In our previous study, adenovirus mediated CTLA4Ig transgene (Ad-CTLA4Ig) therapy was demonstrated to improve pregnancy outcome in an abortion-prone mouse model by skewing the Th2/Th1 c...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2013.01.002
更新日期:2013-04-01 00:00:00
abstract::Members of the galectin family are expressed within the female reproductive tract and have been shown to be involved in multiple biological functions that support the progression of pregnancy. Specific expression patterns of different members of this family have been identified at the maternal decidua and on the place...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章,评审
doi:10.1016/j.jri.2013.05.005
更新日期:2014-03-01 00:00:00
abstract::The immune reactivity of lymphoid cells from pregnant mice was studied during the course of pregnancy in primiparous and multiparous animals either " isopregnant " (male and female of same strain) or " allopregnant " (male and female differing at H-2), using a local GVH assay (CBA lymphoid cells injected into (CBA X A...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(84)90024-x
更新日期:1984-05-01 00:00:00
abstract::An elevated level of insulin growth factor (IGF-1) in rat uterine fluid has been shown to exert detrimental effects of embryo development possibly leading to an increase in pregnancy loss. Interestingly, the administration of somatostatin to rats undergoing superovulation reduced IGF-1 levels in uterine luminal fluid ...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2018.03.002
更新日期:2018-06-01 00:00:00
abstract::Antisera to Mason-Pfizer or Baboon Endogenous Virus possessed complement-dependent cytotoxicity for cell lines chronically infected with these viruses, with some degree of cross-reaction. When appropriately absorbed with virus-free cells, the antisera were not cytotoxic for lymphocytes of adult males but lysed lymphoc...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(81)90001-2
更新日期:1981-03-01 00:00:00
abstract::Endometriosis is a chronic benign disease that affects women of reproductive age causing abdominal pain and infertility. Its pathogenesis remains obscure despite all the research conducted over the past 100 years. However, there is a consensus among the specialists that the basis of its pathophysiology would be multif...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章,评审
doi:10.1016/j.jri.2013.03.003
更新日期:2013-06-01 00:00:00
abstract::We have investigated serum and intracavitary levels of IL-1 alpha, IL-1 beta and IL-1ra from agonadal women undergoing mock cycles (n = 20) of oocyte donation as a clinical model of controlled hormonal stimulation. Further, we compared the intracavitary IL-1 alpha, IL-1 beta and IL-1ra levels in the microenvironment o...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(96)00982-5
更新日期:1996-10-01 00:00:00
abstract::Lactoferrin (LF) and transferrin (Trf) are glycoproteins with strong affinities for ferric ions. Human syncytiotrophoblastic membranes analyzed by enzyme linked immunosorbent assay (ELISA) and immunoblotting were negative with monoclonal and polyclonal antibodies to LF. Immunohistological studies of 35 normal placenta...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(93)90024-c
更新日期:1993-01-01 00:00:00
abstract::This study has assessed the immunological and biological efficacy of two different doses of a recombinant GnRH vaccine administered to intact domestic cats. Fifteen kittens, 8-9 weeks of age, were allocated to three unequal treatment groups: group 1, 1 male control cat; group 2, 5 females administered 10 microg of GnR...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2004.08.004
更新日期:2004-12-01 00:00:00
abstract::The majority of anti-sperm monoclonal antibodies which were shown to inhibit in vitro fertilization in our laboratory react with antigens in the acrosomal region of spermatozoa. To elucidate the mechanism of human and/or mouse fertilization inhibition, the effects of these antibodies on mouse sperm-zona binding and mo...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(92)90023-w
更新日期:1992-03-01 00:00:00
abstract::A large clinical literature suggests that estradiol (E(2)) plays a critical role in immune function. To further explore the relationship between E(2) and immune function, we examined a variety of immunological parameters in a rhesus monkey model of menopause and hormone replacement therapy. Rhesus monkeys (Age, 13.7+/...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/s0165-0378(00)00087-5
更新日期:2001-04-01 00:00:00