Identification of a histamine-releasing factor secreted by human pre-implantation embryos grown in vitro.


:Human pre-implantation stage embryos cultured in vitro spontaneously secreted a factor capable of inducing histamine-release from human blood basophils. The embryo-derived histamine-releasing factor (EHRF) has been isolated from the culture medium by means of heparin-Sepharose affinity chromatography. The factor bound to the column and was then eluted by increasing the buffer molarity to 1.5 M NaCl. EHRF was detected using an enzymatic-isotopic microassay and sensitized basophils known to undergo release with anti-IgE. The EHRF-induced histamine-release was calcium and temperature dependent and the relatively slow kinetics (10 min) were similar to those obtained with anti-IgE. EHRF caused the release of a substantial amount of histamine (48%, n = 18) in a dose-dependent manner. The equivalent fraction isolated from medium containing unfertilized oocytes gave less than 10% of histamine-release using the same source of basophils, suggesting that EHRF was secreted after fertilization. EHRF was very stable since it was resistant to boiling, lyophilization, and to several freeze and thaw treatments. The histamine-releasing activity induced by EHRF was measured in vitro also by means of purified leukocytes containing sensitized basophils. EHRF could represent a message sent by the embryo to the mother to induce histamine release at the time of implantation.


J Reprod Immunol


Cocchiara R,Di Trapani G,Azzolina A,Albeggiani G,Geraci D




Has Abstract


1988-06-01 00:00:00














  • Reflections upon immunological mechanisms involved in fertility, pregnancy and parasite infections.

    abstract::During a pregnancy, the mother accepts her semi-allogeneic fetus with no signs of immunological rejection. Therefore, some modulation of the maternal immune system must occur. Similarly, changes in the host's immune system occurs during infections with parasites. In a study conducted in an endemic area in Bolivia, it ...

    journal_title:Journal of reproductive immunology

    pub_type: 杂志文章,评审


    authors: Persson G,Ekmann JR,Hviid TVF

    更新日期:2019-11-01 00:00:00

  • Evidence for the expression of granulocyte-macrophage colony-stimulating factor receptors by human first trimester extravillous trophoblast and its response to this cytokine.

    abstract::The present study has demonstrated that human extravillous trophoblast cells isolated from first trimester placentae can bind granulocyte-macrophage colony-stimulating factor (GM-CSF). We have used a technique which incorporates a phycoerythrin (PE)-conjugated GM-CSF in association with a monoclonal antibody against t...

    journal_title:Journal of reproductive immunology

    pub_type: 杂志文章


    authors: Loke YW,King A,Gardner L,Carter NP

    更新日期:1992-06-01 00:00:00

  • Molecular signature and functional analysis of uterine ILCs in mouse pregnancy.

    abstract::In addition to natural killer cells, other innate lymphoid cells have recently been identified in the mouse and human uterus, but their roles in successful pregnancy remain poorly defined. In this study, we examined the dynamic changes of uterine innate lymphoid cells throughout pregnancy in mice. We found that the to...

    journal_title:Journal of reproductive immunology

    pub_type: 杂志文章


    authors: Li M,Gao Y,Yong L,Huang D,Shen J,Liu M,Ren C,Hou X

    更新日期:2017-09-01 00:00:00

  • Influence of immunization with allogeneic spleen cells on the number of viable neonates in mice.

    abstract::Female CBA/J (H-2k) mice mated with male DBA/2J (H-2d) mice show a high level of fetal resorption, which can be reduced by immunization with BALB/c (H-2d) spleen cells. The morphologically defined fetal resorption rate upon which evaluation of the outcome of pregnancy has previously been based in this strain combinati...

    journal_title:Journal of reproductive immunology

    pub_type: 杂志文章


    authors: Heine O,Neppert J,Mueller-Eckhardt G

    更新日期:1989-05-01 00:00:00

  • Estrogen and thymic hormone interactions in the female mouse.

    abstract::This study showed that there are relationships between thymic and adrenal/ovarian endocrine actions. A 4 mg/kg dose of thymosin fraction 5 (TSN5) advanced vaginal opening and elevated estrogen levels. Single estradiol benzoate (EB) injections decreased thymus weight and caused a transient reduction in circulating plas...

    journal_title:Journal of reproductive immunology

    pub_type: 杂志文章


    authors: Allen LS,McClure JE,Goldstein AL,Barkley MS,Michael SD

    更新日期:1984-01-01 00:00:00

  • Peptide presentation and NK inhibition by HLA-G.

    abstract::The expression of the nonclassical MHC class Ib molecule HLA-G is nearly exclusively restricted to the feto-maternal interface during pregnancy. There it probably serves the same physiological functions already known for classical MHC class I molecules; these include peptide presentation, natural killer cell (NK) inhi...

    journal_title:Journal of reproductive immunology

    pub_type: 杂志文章


    authors: Münz C,Stevanović S,Rammensee HG

    更新日期:1999-07-01 00:00:00

  • Immunological reactivity of maternal lymphocytes during normal pregnancy in the rat.

    abstract::Thoracic duct lymphocytes from pregnant PVG rats differed markedly from similar cells collected from non-pregnant donors in their reactivity in popliteal lymph node assays performed in (PVG x DA) F1 hybrid hosts. Cells from pregnant rats failed to evoke an increase in popliteal node size commensurate with higher chall...

    journal_title:Journal of reproductive immunology

    pub_type: 杂志文章


    authors: McCullagh P

    更新日期:1981-05-01 00:00:00

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    abstract::The objective of these experiments was to determine the effects of neonatal thymectomy on the induction of experimental autoimmune orchitis in inbred rats of the Fischer 344 and Lewis strains. It was found that thymectomy alone in Lewis rats, and thymectomy followed by immunization with testicular extract in both Lewi...

    journal_title:Journal of reproductive immunology

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    authors: Lipscomb HL,Gardner PJ,Sharp JG

    更新日期:1979-12-01 00:00:00

  • Spliceosome protein EFTUD2 is upregulated in the trophoblast of spontaneous miscarriage and hydatidiform mole.

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    journal_title:Journal of reproductive immunology

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    authors: Löb S,Vattai A,Kuhn C,Schmoeckel E,Mahner S,Wöckel A,Kolben T,Szekeres-Bartho J,Jeschke U,Vilsmaier T

    更新日期:2020-08-01 00:00:00

  • Testis physiology relevant to immunoregulation.

    abstract::Intra-testicular transplants are placed in rodents into the large lymphatic sinusoids of the interstitial tissue of the testis. These sinusoids are filled with a protein-rich extracellular fluid that supplies all the requirements of the grafts until vascularization takes place. The testicular microvascular endothelium...

    journal_title:Journal of reproductive immunology

    pub_type: 杂志文章,评审


    authors: Setchell BP,Uksila J,Maddocks S,Pöllänen P

    更新日期:1990-08-01 00:00:00

  • 17 beta-estradiol induces L-type Ca2+ channel activation and regulates redox function in macrophages.

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    journal_title:Journal of reproductive immunology

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    authors: Azenabor AA,Chaudhry AU

    更新日期:2003-06-01 00:00:00

  • Intrauterine infusion of bacterial lipopolysaccharide (LPS) prior to mating has no adverse effect on fertility, fetal survival and fetal development.

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    journal_title:Journal of reproductive immunology

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    authors: Tuo W,Ott TL,Liu S,Bazer FW

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  • Pravastatin prevents miscarriages in antiphospholipid antibody-treated mice.

    abstract::Miscarriages in patients with antiphospholipid (aPL) antibodies have been attributed to thrombosis of placental vessels. However, we have shown that inflammation plays a crucial role in fetal injury. We identified tissue factor (TF), the major cellular activator of the coagulation cascade, as a key mediator in inflamm...

    journal_title:Journal of reproductive immunology

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    authors: Girardi G

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  • Trophoblast debris modulates the expression of immune proteins in macrophages: a key to maternal tolerance of the fetal allograft?

    abstract::Interactions between maternal immune cells and the placenta are of substantial interest since diseases of pregnancy, such as recurrent miscarriage, villitis of unknown etiology and preeclampsia may arise due to inadequate adaptation of the maternal immune system. During normal pregnancy trophoblast debris is shed from...

    journal_title:Journal of reproductive immunology

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    authors: Abumaree MH,Chamley LW,Badri M,El-Muzaini MF

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    journal_title:Journal of reproductive immunology

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    authors: Buckingham KL,Chamley LW

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    journal_title:Journal of reproductive immunology

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    authors: Rote NS,Lau RJ,Harrison MR,Branch DW,Scott JR

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    abstract::Cytokines seem to play a critical role in the pathogenesis of unexplained recurrent pregnancy loss (RPL). Th1 cytokines have been shown to exert deleterious effects on pregnancy, inhibiting foetal growth and development. On the other hand, Th2 cytokines have been associated with successful pregnancy. The purpose of th...

    journal_title:Journal of reproductive immunology

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    authors: Daher S,de Arruda Geraldes Denardi K,Blotta MH,Mamoni RL,Reck AP,Camano L,Mattar R

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  • Usefulness of synthetic Mimetic ligands in establishing an affinity chromatography procedure for purifying estradiol antibody in sheep serum: potential application in regulating fertility of the ram.

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    journal_title:Journal of reproductive immunology

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    authors: Squire RS,Greenwood JV,Sanford LM

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    abstract::Supernatants from mixed lymphocyte reactions performed in the presence of pregnancy serum (PS) or normal human serum (NHS) were tested for interleukin-1 (IL-1) activity in a thymocyte assay. A lower response was obtained with PS-containing supernatants, suggesting that PS exerts its suppressive effect by inhibiting IL...

    journal_title:Journal of reproductive immunology

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    authors: Garcia Domingo C,Moreno A,Palomino P

    更新日期:1988-06-01 00:00:00

  • IL-1beta during in vitro decidualization in primate.

    abstract::The sequence of biochemical and molecular events associated with decidualization in the primate remain unclear. In the baboon, the sequential changes during this period in vivo are characterized by the downregulation of alpha-smooth actin followed by induction of cyclooxygenase-2 (COX-2) at the implantation site and t...

    journal_title:Journal of reproductive immunology

    pub_type: 杂志文章,评审


    authors: Strakova Z,Srisuparp S,Fazleabas AT

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  • Suppression of fertility in male mice by immunization against LH receptor.

    abstract::We have investigated the potential contraceptive effects of immunization against the luteinizing hormone (LH) receptor in male mice at the prepubertal stage. Two N-terminal fragments of the porcine LH receptor encoding amino acids 1-297 and 1-370 were produced in large quantities through the Baculovirus insect cell sy...

    journal_title:Journal of reproductive immunology

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    authors: Remy JJ,Bozon V,Couture L,Goxe B,Salesse R,Garnier J

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  • Characterisation of the soluble fraction of human syncytiotrophoblast microvillous plasma membrane-associated proteins.

    abstract::Syncytiotrophoblast microvillous plasma membrane (StMPM) preparations were obtained from human full-term placentae by previously published methods of cold saline extraction and phase centrifugation. Purity of these preparations was assessed by electron microscopy, enzyme analysis and hydroxyproline content. IgG, album...

    journal_title:Journal of reproductive immunology

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    authors: Ogbimi AO,Johnson PM,Brown PJ,Fox H

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  • Constitutive or induced elevated levels of L-carnitine correlate with the cytokine and cellular profile of endometriosis.

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    journal_title:Journal of reproductive immunology

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    authors: Dionyssopoulou E,Vassiliadis S,Evangeliou A,Koumantakis EE,Athanassakis I

    更新日期:2005-04-01 00:00:00

  • Intrauterine administration of autologous hCG- activated peripheral blood mononuclear cells improves pregnancy outcomes in patients with recurrent implantation failure; A double-blind, randomized control trial study.

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    journal_title:Journal of reproductive immunology

    pub_type: 杂志文章


    authors: Pourmoghadam Z,Soltani-Zangbar MS,Sheikhansari G,Azizi R,Eghbal-Fard S,Mohammadi H,Siahmansouri H,Aghebati-Maleki L,Danaii S,Mehdizadeh A,Hojjat-Farsangi M,Motavalli R,Yousefi M

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  • Foetal immune programming: hormones, cytokines, microbes and regulatory T cells.

    abstract::In addition to genetic factors, environmental cues play important roles in shaping the immune system. The first environment that the developing foetal immune system encounters is the uterus. Although physically the mother and the foetus are separated by the placental membranes, various factors such as hormones and cyt...

    journal_title:Journal of reproductive immunology

    pub_type: 杂志文章,评审


    authors: Hsu P,Nanan R

    更新日期:2014-10-01 00:00:00

  • LPS mediated production of IL-1, PGE2 and PGF2alpha from term decidua involves tumour necrosis factor and tumour necrosis factor receptor p55.

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    journal_title:Journal of reproductive immunology

    pub_type: 杂志文章


    authors: Arntzen KJ,Egeberg K,Rahimipoor S,Vatten L,Austgulen R

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  • Analysis of immunoregulatory activity of a choriocarcinoma-derived factor: specific suppression of proliferative process of cell-mediated immune responses including LAK cell generation.

    abstract::The immunosuppressive activity of a JEG-3 choriocarcinoma-derived factor in human IL-2-dependent T cell responses has been studied, together with its effect on IL-2-independent T cell responses induced by 10 nM TPA. The factor completely suppressed the IL-2-independent proliferative responses of T cells but failed to ...

    journal_title:Journal of reproductive immunology

    pub_type: 杂志文章


    authors: Matsuzaki N,Saji F,Okada T,Sawai K,Kameda T,Tanizawa O

    更新日期:1991-02-01 00:00:00

  • Galectin signature in normal pregnancy and preeclampsia.

    abstract::Members of the galectin family are expressed within the female reproductive tract and have been shown to be involved in multiple biological functions that support the progression of pregnancy. Specific expression patterns of different members of this family have been identified at the maternal decidua and on the place...

    journal_title:Journal of reproductive immunology

    pub_type: 杂志文章,评审


    authors: Blois SM,Barrientos G

    更新日期:2014-03-01 00:00:00

  • Characterization of myeloid cell populations in human testes collected after sex reassignment surgery.

    abstract::The testis has been described in animal models as a site of immune privilege, which protects spermatids against tissue damage during inflammation. Myeloid cells, including macrophages and dendritic cells (DC), are defined as key players in the testicular immune privilege in animal models. However, their distribution a...

    journal_title:Journal of reproductive immunology

    pub_type: 杂志文章


    authors: Ponte R,Dupuy FP,Brimo F,Mehraj V,Brassard P,Belanger M,Yurchenko E,Jenabian MA,Bernard NF,Routy JP,ORCHID study group.

    更新日期:2018-02-01 00:00:00

  • Interleukin 1 beta is induced by interleukin 11 during decidualization of human endometrial stromal cells, but is not released in a bioactive form.

    abstract::Blastocyst implantation is dependent on the differentiation of endometrial stromal cells (ESC) into decidual cells. Decidualization of human ESC in vitro is enhanced by interleukin 11 (IL11), with associated changes in gene expression. Genes downstream of IL11 may provide targets for the treatment of implantation fail...

    journal_title:Journal of reproductive immunology

    pub_type: 杂志文章


    authors: White CA,Dimitriadis E,Sharkey AM,Stoikos CJ,Salamonsen LA

    更新日期:2007-02-01 00:00:00