Abstract：
:The feasibility of producing epitope-specific antigens by mutation of the gene is demonstrated, the aim being to eliminate unwanted surface epitopes yet allowing the natural folding of the protein to maintain the desired epitope(s). The model protein is the beta subunit of human chorionic gonadotropin (hCG beta) which previously has been used as an immunological contraceptive vaccine but has extensive cross-reaction with human luteinizing hormone. Of a series of mutants made, the mutant with substitutions of Glu for Arg 68, Ser for Arg 74, His for Gly 75 and His for Val 79, lost the ability to react with a panel of cross-reacting monoclonal antibodies while retaining the discontinuous and linear epitopes specific to the holo-hormone. In addition, allocation of amino acid residues to established epitope clusters could be made: residues 24, 25, 68 and 71 probably contribute to the cluster termed beta 3, residues 20, 21, 22, 75 and 77 to cluster beta 6 and residue 68 to clusters beta 2, beta 4 and beta 5.
journal_name
J Reprod Immunoljournal_title
Journal of reproductive immunologyauthors
Jackson AM,Klonisch T,Lapthorn AJ,Berger P,Isaacs NW,Delves PJ,Lund T,Roitt IMdoi
10.1016/0165-0378(96)00978-3subject
Has Abstractpub_date
1996-08-01 00:00:00pages
21-36issue
1-2eissn
0165-0378issn
1872-7603pii
0165-0378(96)00978-3journal_volume
31pub_type
杂志文章abstract：:Prolactin (PRL) influences immune reactivity in animals and in humans and both T-cell abnormalities and reduced natural killer (NK) cell activity have been reported in women with pathological hyperprolactinemia. To investigate further the possible interactions between PRL and the immune system in humans, we analysed T...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/0165-0378(89)90031-4
更新日期：1989-05-01 00:00:00
abstract：:During syngeneic pregnancy the numbers of 'spontaneously' occurring ('background') Ig-secreting cells were determined in the spleen, bone marrow (BM) and mesenteric lymph nodes (MLN) of BALB/c mice that were kept under germfree conditions and fed a low molecular weight synthetic diet (GF-CD), SPF BALB/c mice fed autoc...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/0165-0378(86)90017-3
更新日期：1986-11-01 00:00:00
abstract：:There is increasing evidence to support the view that human parturition represents an inflammatory process. We have previously demonstrated that parturition is associated with leukocyte invasion and pro-inflammatory cytokine production in the cervix and myometrium. Furthermore, we have shown that several cell adhesion...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/j.jri.2004.04.003
更新日期：2004-08-01 00:00:00
abstract：:17 beta-estradiol induces rapid effects on cells of the immune system via plasma membrane surface receptor but the ways in which delayed signals involving intracellular receptors affect the same functions are not well understood. To study the delayed but sustained events in estradiol signaling, we have investigated ma...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/s0165-0378(02)00084-0
更新日期：2003-06-01 00:00:00
abstract：:To analyze immunomodulating effects related to parity status, we studied trophoblast invasion grade, placental expression and systemic concentration of VEGF and its receptor Flt-1 in normal fertile (CBA/JxBALB/c) mice and abortion-prone (CBA/JxDBA/2) H-2(d)xH-2(k) mice. BALB/c or DBA/2 mated CBA/J females were, respec...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/j.jri.2010.03.004
更新日期：2010-06-01 00:00:00
abstract：:The NOD mouse is a model for autoimmune diabetes that develops symptoms similar to Type I diabetes. The incidence of diabetes is greater in females but the degree of insulitis is comparable in both sexes. The purpose of this study was to assess the populations of lymphocytes and macrophages in the pancreas and spleen ...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/s0165-0378(96)00986-2
更新日期：1996-11-01 00:00:00
abstract：:A maternal low-protein (LP) diet in Sprague-Dawley rats results in low birth weight, rapid adipose tissue catch-up growth, adult obesity, and insulin resistance. The placenta functions to fulfill the fetus' nutrient demands. Adequate angiogenic factor concentrations help to ensure normal growth and vasculature develop...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/j.jri.2016.08.009
更新日期：2016-11-01 00:00:00
abstract：:This study has assessed the immunological and biological efficacy of two different doses of a recombinant GnRH vaccine administered to intact domestic cats. Fifteen kittens, 8-9 weeks of age, were allocated to three unequal treatment groups: group 1, 1 male control cat; group 2, 5 females administered 10 microg of GnR...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/j.jri.2004.08.004
更新日期：2004-12-01 00:00:00
abstract：:The sequence of biochemical and molecular events associated with decidualization in the primate remain unclear. In the baboon, the sequential changes during this period in vivo are characterized by the downregulation of alpha-smooth actin followed by induction of cyclooxygenase-2 (COX-2) at the implantation site and t...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章,评审
doi：10.1016/s0165-0378(01)00141-3
更新日期：2002-05-01 00:00:00
abstract：:In vitro fertilization (IVF) pregnancies potentially have a higher rate of preterm delivery (PTD) than do spontaneously conceived gestations, and differences persist following adjustment for multiple gestation, maternal age, and parity. The reasons for this increased susceptibility to PTD remain incompletely elucidate...
journal_title：Journal of reproductive immunology
pub_type： 临床试验,杂志文章
doi：10.1016/j.jri.2015.05.004
更新日期：2015-11-01 00:00:00
abstract：:The cross reactivities of acidic extracts from boar, bovine, hamster, human and ram spermatozoa to a polyclonal antibody of the boar proacrosin binding protein has been investigated. The pH 3.0 extracts of the washed spermatozoa from each species were subjected to Western blot analysis using a polyclonal antibody deve...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/0165-0378(92)90033-z
更新日期：1992-04-01 00:00:00
abstract：:Galectin-1 (gal-1), a member of a family of conserved β-galactoside-binding proteins, has been shown to exert a key role during gestation. Though gal-1 is expressed at higher levels in the placenta from HELLP patients, it is still poorly understood whether systemic gal-1 levels also differ in HELLP patients. In the pr...
journal_title：Journal of reproductive immunology
pub_type： 临床试验,杂志文章
doi：10.1016/j.jri.2016.02.002
更新日期：2016-04-01 00:00:00
abstract：:Inbred mice from selected unrelated and congenic strains were mated to determine the relative effects of maternal-fetal disparity at major histocompatibility complex (H-2) and non-H-2 minor histocompatibility antigens on the feto-placental unit at 14 days of gestation. A significant increase in weight of the feto-plac...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/0165-0378(85)90045-2
更新日期：1985-11-01 00:00:00
abstract：:We have previously shown that in patients with idiopathic premature ovarian failure there were significant changes in lymphocyte subsets. To test our hypothesis that these changes were due to oestrogen deficiency we studied lymphocyte subsets in patients with oestrogen deficiency due to other causes. Blood was taken f...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/0165-0378(91)90025-l
更新日期：1991-05-01 00:00:00
abstract：:Development of a vaccine(s) based on sperm antigens represents a promising approach to contraception. The utility of an antigen in immunocontraception is contingent upon sperm/testis-specificity and its involvement in the fertilization process. Since, the sperm-zona pellucida (ZP) recognition and binding constitutes t...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/s0165-0378(01)00094-8
更新日期：2002-01-01 00:00:00
abstract：:Few studies have assessed longitudinal changes in circulating cytokine levels during normal pregnancy. We have examined the natural history of maternal plasma cytokines from early- to mid-pregnancy in a large, longitudinal cohort. Multiplex flow cytometry was used to measure interleukin (IL)-2, IL-6, IL-12, tumor necr...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/j.jri.2007.06.051
更新日期：2008-04-01 00:00:00
abstract：:Placental culture supernatants (PS) obtained from various mouse crossbreedings were added to mouse IgG1 hybridoma cultures producing anti-DNP antibodies. The quantity of monoclonal antibody (mAb) produced, the nature of these antibodies and the proliferation of the hybridoma cells were studied. It was observed that th...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/0165-0378(96)00958-8
更新日期：1996-05-01 00:00:00
abstract：:Cationic host defence peptides (CHDP; also known as antimicrobial peptides) are key components of the immune response in the female reproductive tract. The role of the placental trophoblast in ovine host defence remains poorly understood. This study characterises expression of genes for cathelicidin and defensin pepti...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/j.jri.2016.06.006
更新日期：2016-09-01 00:00:00
abstract：:Cytokines present in the reproductive system play an important role both in the modulation of immune responses to infectious challenge and in the establishment and maintenance of pregnancy. Interleukin 18 (IL-18) has been regarded as an important regulator of innate and acquired immune response, but its expression and...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/j.jri.2011.07.002
更新日期：2011-12-01 00:00:00
abstract：:Currently, different approaches are used to select oocytes for in vitro fertilization (IVF) procedures, but they do not assure a significant association with the pregnancy outcome. Since several studies have proposed the expression of HLA-G antigens in early embryos to be a possible marker of elevated implantation rat...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/j.jri.2007.02.005
更新日期：2007-06-01 00:00:00
abstract：:Throughout follicular growth the number of immune cells increases, enhanced under stimulation with exogenous gonadotropins. This treatment, however, may adversely influence folliculogenesis and negatively affect oocyte quality through modifications in the follicular concentrations of cytokines released by these immune...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/j.jri.2014.06.003
更新日期：2014-12-01 00:00:00
abstract：:The process of ovarian follicular development is a long and arduous one, marked by dramatic proliferation and differentiation of both the somatic and germ cell elements. To a large extent, this explosive agenda is under the control of conventional endocrine principles, involving hormones such as pituitary gonadotropin...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章,评审
doi：10.1016/s0165-0378(98)00026-6
更新日期：1998-08-01 00:00:00
abstract：:CD14, the major receptor for bacterial lipopolysaccharide (LPS) as well as other microbial antigens, is a component of the innate immune system. We hypothesized that a single nucleotide C>T polymorphism at position -159 in the CD14 gene that results in elevated CD14 production would influence susceptibility to preterm...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/j.jri.2005.12.002
更新日期：2006-06-01 00:00:00
abstract：:Reproductive immunology applies general immunology principles to specialised targets, reproduction and development. The involvement of colony-stimulating factors (CSFs) in reproduction illustrates this. The CSF family includes CSF-1 or macrophage CSF (M-CSF), CSF-2 or granulocyte macrophage CSF (GM-CSF), and CSF-3 or ...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章,评审
doi：10.1016/j.jri.2015.01.005
更新日期：2015-06-01 00:00:00
abstract：:Lymphohematopoietic cytokines play a significant role in many biological mechanisms including a number of reproductive processes such as ovulation, implantation, placentation, cervical dilation and parturition. Recent experiments have suggested that cytokines play a crucial role in the mechanisms of preterm labor and ...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章,评审
doi：10.1016/s0165-0378(00)00064-4
更新日期：2000-07-01 00:00:00
abstract：:Menstrual toxic shock syndrome (mTSS) is an acute systemic inflammatory disease associated with the superantigenic exotoxin, toxic shock syndrome toxin (TSST)-1, produced by Staphylococcus aureus and the use of high absorbency tampons. Even though S. aureus is capable of elaborating several other superantigenic exotox...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/j.jri.2006.06.007
更新日期：2007-02-01 00:00:00
abstract：:For many years, experience has been accumulated on embryo and gamete manipulation in livestock animals. The present work is a review of these techniques and their possible application in human embryology in specific cases. It is possible to manipulate gametes at different levels, producing paternal or maternal haploid...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章,评审
doi：10.1016/s0165-0378(01)00138-3
更新日期：2002-05-01 00:00:00
abstract：:A monoclonal anti-human sperm antibody (Mab 1A1) has been produced by fusion of myeloma cells with splenocytes from a BALB/c mouse immunized with in vitro capacitated human spermatozoa. Immunofluorescence studies with Mab 1A1 show that it recognizes an antigen(s) (Ag 1A1) which is located in the acrosome of human sper...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/0165-0378(91)90039-s
更新日期：1991-04-01 00:00:00
abstract：:The process of embryo attachment and invasion through the endometrial epithelial cells and subsequent implantation into the decidualized endometrial stroma is the groundbreaking step for the establishment of a successful pregnancy. Necessary prerequisites are a receptive endometrium, a good-quality embryo and a well-o...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章,评审
doi：10.1016/j.jri.2015.02.002
更新日期：2015-04-01 00:00:00
abstract：:Endometriosis is a benign, chronic inflammatory disease that presents alterations in immune response that can be detected in eutopic endometrium, peritoneal fluid and peripheral blood of affected women. Regulatory T (TReg) cells are a subpopulation of T lymphocytes specialized in immune regulation that seem to partici...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章,评审
doi：10.1016/j.jri.2017.04.003
更新日期：2017-04-01 00:00:00
