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Identification and characterization of human acrosomal antigen defined by a monoclonal antibody with blocking effect on in vitro fertilization.

Abstract：

:A monoclonal anti-human sperm antibody (Mab 1A1) has been produced by fusion of myeloma cells with splenocytes from a BALB/c mouse immunized with in vitro capacitated human spermatozoa. Immunofluorescence studies with Mab 1A1 show that it recognizes an antigen(s) (Ag 1A1) which is located in the acrosome of human spermatozoa. As shown by Western blotting experiments, 1A1 antigen represents a family of proteins with Mr ranging from 20 kDa to 34 kDa. Immunofluorescence observations on epitope exposure and location suggest that during in vitro capacitation of human spermatozoa, Mab 1A1 epitope-bearing molecules are concentrated in regularly arranged granules in the acrosome. After long-term incubation the epitope is exposed on the apical acrosome surface exhibiting a spot-like arrangement. The 1A1 epitope is widely distributed among mammalian species: boar, ram, mouse and rat acrosome is intensively stained by Mab 1A1. The antibody inhibits in vitro fertilization mainly by blocking sperm attachment to and penetration through the zona pellucida when included in the medium for the in vitro fertilization of mouse, porcine and human oocytes.

journal_name

J Reprod Immunol

journal_title

Journal of reproductive immunology

authors

Dubova-Mihailova M,Mollova M,Ivanova M,Kehayov I,Kyurkchiev S

doi

10.1016/0165-0378(91)90039-s

subject

Has Abstract

pub_date

1991-04-01 00:00:00

pages

251-68

issue

3

eissn

0165-0378

issn

1872-7603

pii

0165-0378(91)90039-S

journal_volume

19

pub_type

杂志文章

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