Unanticipated changes in steady-state mRNA levels for glyceraldehyde-3-phosphate dehydrogenase in rat tibiae.

Abstract:

:Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA levels are commonly used as an internal control to normalize gene expression data based on the belief that this gene is constitutively expressed. However, GAPDH mRNA levels increased by more than 2.5-fold in tibiae of hindlimb unloaded female rats compared to L32 mRNA levels. Similarly, GAPDH mRNA levels increased compared to 18S ribosomal RNA. Treatment with growth hormone and alcohol show no disparity in GAPDH mRNA levels whereas in some experiments, parathyroid hormone and 17beta-estradiol increased GAPDH mRNA levels. Taken together, these findings indicate that it is essential to demonstrate that GAPDH expression is not altered prior to using the gene as an internal control.

journal_name

Calcif Tissue Int

authors

Maran A,Hefferan TE,Zhang M,Turner RT

doi

10.1007/s00223-002-1098-2

subject

Has Abstract

pub_date

2004-02-01 00:00:00

pages

204-7

issue

2

eissn

0171-967X

issn

1432-0827

journal_volume

74

pub_type

杂志文章
  • The Predictive Value of Sarcopenia and Falls for 2-Year Major Osteoporotic Fractures in Community-Dwelling Older Adults.

    abstract::To evaluate the associations of sarcopenia and previous falls with 2-year major osteoporotic fractures (MOFs) in community-dwelling older adults. Four thousand Chinese men and women ≥ 65 years recruited from Hong Kong communities were prospectively followed up. Measures of muscle mass, grip strength, gait speed and fa...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-020-00709-z

    authors: Su Y,Lam FMH,Leung J,Cheung WH,Ho SC,Kwok T

    更新日期:2020-08-01 00:00:00

  • Long-term treatment with eldecalcitol (1α, 25-dihydroxy-2β- (3-hydroxypropyloxy) vitamin D3) suppresses bone turnover and leads to prevention of bone loss and bone fragility in ovariectomized rats.

    abstract::The purpose of this study is to estimate the efficacy of eldecalcitol (1α, 25-Dihydroxy-2β- (3-hydroxypropyloxy) vitamin D3; ELD) on bone metabolism after long-term administration. Six-month-old Wistar-Imamichi rats were ovariectomized (OVX) and administered ELD orally at doses of 7.5, 15, or 30 ng/kg daily. Bone mine...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-014-9937-5

    authors: Takeda S,Smith SY,Tamura T,Saito H,Takahashi F,Samadfam R,Haile S,Doyle N,Endo K

    更新日期:2015-01-01 00:00:00

  • Effects of aluminum(III), chromium(III), and iron(III) on the rate of dissolution of calcium hydroxyapatite crystals in the absence and presence of the chelating agent desferrioxamine.

    abstract::Aluminum ions (Al) and chromium (III) ions (Cr), as they exist in aqueous solution at neutral pH, adsorb onto calcium hydroxyapatite crystals (HAP) and severely inhibit their dissolution process, when present in concentrations less than 1 microM. Iron (III) ions (Fe), at concentrations up to 10 microM, have no effect ...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/BF02555127

    authors: Christoffersen MR,Thyregod HC,Christoffersen J

    更新日期:1987-07-01 00:00:00

  • Incorporation of (35S)sulfate and (3H)glucosamine into glycoaminoglycans in rat incisor predentine and dentine: comparison by autoradiography of fixation by rapid-freezing, freeze-substitution, and aldehyde fixation.

    abstract::At 4 and 24 hours after injection of (35S)sulfate, there were 22-67% fewer silver grains on conventionally fixed sections than on sections fixed by cryotechniques. Differences were smaller when (3H) glucosamine was chosen as precursor. The number of silver grains increased between 4 and 24 hours in predentine and in a...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/BF02557834

    authors: Goldberg M,Escaig F

    更新日期:1985-09-01 00:00:00

  • The effect of intravenous pamidronate on bone mineral density, bone histomorphometry, and parameters of bone turnover in adults with type IA osteogenesis imperfecta.

    abstract::The type IA osteogenesis imperfecta (OI) phenotype is characterized by multiple fractures, blue sclerae, and minimal skeletal deformity without dentinogenesis imperfecta. The object of this study was to determine the effect of treatment with intravenous pamidronate (30 mg) every 3 months on bone density and bone histo...

    journal_title:Calcified tissue international

    pub_type: 临床试验,杂志文章

    doi:10.1007/s00223-001-1055-5

    authors: Shapiro JR,McCarthy EF,Rossiter K,Ernest K,Gelman R,Fedarko N,Santiago HT,Bober M

    更新日期:2003-02-01 00:00:00

  • Compressive strength, ash weight, and volume of vertebral trabecular bone in experimental fluorosis in pigs.

    abstract::The aim of the investigation was to measure the effect of fluoride on vertebral trabecular bone compressive strength and to correlate this with fluoride-induced changes in bone density. This correlation would express changes in the quality of bone during fluoride treatment. Pigs were used in the experiment because the...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/BF02556693

    authors: Mosekilde L,Kragstrup J,Richards A

    更新日期:1987-06-01 00:00:00

  • HNGF6A Inhibits Oxidative Stress-Induced MC3T3-E1 Cell Apoptosis and Osteoblast Phenotype Inhibition by Targeting Circ_0001843/miR-214 Pathway.

    abstract::Humanin (HN), a mitochondrial derived peptide, plays cyto-protective role under various stress. In this study, we aimed to investigate the effects of HNGF6A, an analogue of HN, on osteoblast apoptosis and differentiation and the underlying mechanisms. Cell proliferation of murine osteoblastic cell line MC3TC-E1 was ex...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-020-00660-z

    authors: Zhu X,Zhao Z,Zeng C,Chen B,Huang H,Chen Y,Zhou Q,Yang L,Lv J,Zhang J,Pan D,Shen J,Duque G,Cai D

    更新日期:2020-05-01 00:00:00

  • Influence of body mass index on the age-related slope of total and regional bone mineral content.

    abstract::The influence of body mass index (BMI) on T scores for total body bone mineral content (TBBMC) and regional bone mineral content (RBMC) was studied in 186 healthy women: 100 postmenopausal, 35 perimenopausal, and 51 premenopausal. The three groups were divided by BMI >25 kg/m2 and BMI <25 kg/m2 and the postmenopausal ...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s002239900310

    authors: Revilla M,Villa LF,Sánchez-Atrio A,Hernández ER,Rico H

    更新日期:1997-08-01 00:00:00

  • Absence of linkage for bone mineral density to chromosome 12q12-14 in the region of the vitamin D receptor gene.

    abstract::Polymorphisms in the region of the gene for the vitamin D receptor (VDR) (chromosome 12q12-14) have been associated with differences in bone mineral density (BMD) in some studies but not in others. Because linkage analysis assesses allele sharing identical-by-descent among relatives instead of the association of a par...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s002230001175

    authors: Zee RY,Myers RH,Hannan MT,Wilson PW,Ordovas JM,Schaefer EJ,Lindpaintner K,Kiel DP

    更新日期:2000-12-01 00:00:00

  • Effects of pleiotrophin (PTN) over-expression on mouse long bone development, fracture healing and bone repair.

    abstract::Pleiotrophin (PTN) was found to have potent effects on regulation of osteoblast recruitment, proliferation and differentiation. The present study examined the long-term effects of targeted PTN over-expression on bone development and repair in a transgenic mouse model. Femurs and tibiae from the PTN transgenic mice and...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-004-0145-6

    authors: Li G,Bunn JR,Mushipe MT,He Q,Chen X

    更新日期:2005-04-01 00:00:00

  • Impact of Conventional Medical Therapy on Bone Mineral Density and Bone Turnover in Adult Patients with X-Linked Hypophosphatemia: A 6-Year Prospective Cohort Study.

    abstract::X-linked hypophosphatemia (XLH) is a rare, inheritable disorder manifesting as rickets in children and osteomalacia in adults. While conventional medical treatment with oral phosphate and alfacalcidol is recommended in childhood, it is undecided whether adults should continue therapy. The aim of this 6-year prospectiv...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-017-0363-3

    authors: Shanbhogue VV,Hansen S,Jørgensen NR,Beck-Nielsen SS

    更新日期:2018-03-01 00:00:00

  • Distribution of the perilacunar hypomineralized areas in cortical bone from patients with familial hypophosphatemic (vitamin D-resistant) rickets.

    abstract::The perilacunar areas of low mineral density in microradiographs from cortical bone of patients with hypophosphatemic (vitamin D-resistant) rickets are not evenly distributed throughout the bone tissue. Their frequency and distribution were determined in bone from 9 patients with this disease. It was found that the le...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/BF02441169

    authors: Choufoer JH,Steendijk R

    更新日期:1979-04-17 00:00:00

  • Bone turnover markers and PTH levels in surgical versus natural menopause.

    abstract::In order to assess similarities and differences in women that suffer surgical versus natural menopause, a series of bone, clinical, and biochemical parameters was assayed in a clinical sample of 35 women with surgical menopause and 112 women with natural menopause. Biochemical parameters included hormones [parathyroid...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-003-0054-0

    authors: García-Pérez MA,Moreno-Mercer J,Tarín JJ,Cano A

    更新日期:2004-02-01 00:00:00

  • X-ray microanalysis of teeth from healthy patients and patients with familial hypophosphatemia.

    abstract::Energy-dispersive X-ray microanalysis was used to determine calcium/phosphorous (Ca/P) ratios in undecalcified teeth, and the sulfur (S) content of dentin of decalcified teeth from normal patients and patients with familial hypophosphatemia, in an attempt to determine the effect of phosphorus deficiency. The results s...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/BF02555886

    authors: Daley TD,Jarvis A,Wysocki GP,Kogon SL

    更新日期:1990-12-01 00:00:00

  • Effect of fluoride pretreatment on the solubility of synthetic carbonated apatite.

    abstract::The purpose of this research was to address the following question: How is the solubility of fluoride-pretreated carbonated apatite (CAP) in aqueous acidic media related to the equilibrium solution fluoride and/or the CAP adsorbed fluoride levels? A CAP sample prepared by a precipitation method at 70 degrees C contain...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-001-1069-z

    authors: Barry AB,Zhuang H,Baig AA,Higuchi WI

    更新日期:2003-03-01 00:00:00

  • A histochemical method for the demonstration of calcifying cartilage.

    abstract::The demonstration of histochemical characteristics in calcifying cartilage is fraught with methodological difficulties including the distinction of mineralized from unmineralized cartilage and the demonstration of cell detail in relatively hard tissue. This study uses the decalcified bone matrix-induced enchondral (en...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/BF02555141

    authors: Aaron RK,Jolly G,Ciombor DM,Barrach HJ

    更新日期:1988-10-01 00:00:00

  • High osteoblastic activity in C3H/HeJ mice compared to C57BL/6J mice is associated with low apoptosis in C3H/HeJ osteoblasts.

    abstract::This study sought to confirm that osteoblasts of C3H/HeJ (C3H) mice, which have higher differentiation status and bone-forming ability compared to C57BL/6J (B6) osteoblasts, also have a lower apoptosis level and to test whether the higher differentiation status and bone-forming ability of C3H osteoblasts were related ...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-005-0303-5

    authors: Sheng MH,Lau KH,Mohan S,Baylink DJ,Wergedal JE

    更新日期:2006-05-01 00:00:00

  • Menopausal bone loss is partially regulated by dietary intake of vitamin D.

    abstract::Five years ago we reported results from a cross-sectional study of the effect of nutritional factors on calcium-regulating hormones and bone loss in perimenopausal women. We found an inverse correlation between serum 25-hydroxyvitamin D (25OHD) and immunoreactive parathyroid hormone (PTH), and we postulated that over ...

    journal_title:Calcified tissue international

    pub_type: 临床试验,杂志文章

    doi:10.1007/BF00334543

    authors: Lukert B,Higgins J,Stoskopf M

    更新日期:1992-09-01 00:00:00

  • Radioimmunoassay for human osteocalcin using an antibody raised against the synthetic human (h37-49) sequence.

    abstract::Radioiodination of synthetic human 37-49 osteocalcin requires optimal labeling conditions in order to obtain a maximum of mono- and di-iodinated tracer with little contamination by tri- and tetra-iodinated products or "radio-damage." The antibody raised against osteocalcin(h37-49) had the highest affinity for the C-te...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/BF02555196

    authors: Jüppner H,Schettler T,Giebel G,Wenner S,Hesch RD

    更新日期:1986-11-01 00:00:00

  • A pediatric bone mass scan has poor ability to predict adult bone mass: a 28-year prospective study in 214 children.

    abstract::As the correlation of bone mass from childhood to adulthood is unclear, we conducted a long-term prospective observational study to determine if a pediatric bone mass scan could predict adult bone mass. We measured cortical bone mineral content (BMC [g]), bone mineral density (BMD [g/cm(2)]), and bone width (cm) in th...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-013-9802-y

    authors: Buttazzoni C,Rosengren BE,Tveit M,Landin L,Nilsson JÅ,Karlsson MK

    更新日期:2014-02-01 00:00:00

  • Contribution of collagen and mineral to the elastic anisotropy of bone.

    abstract::It has long been thought that collagen fibers within the bone matrix are deposited in an aligned pattern that channels mineral growth. If this model of bone structure is correct, both organic and inorganic phases of bone should have similar elastic anisotropy. Using an acoustic microscope, we measured longitudinal and...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/BF00299319

    authors: Hasegawa K,Turner CH,Burr DB

    更新日期:1994-11-01 00:00:00

  • Osteocalcin gene expression is regulated by wild-type p53.

    abstract::The tumor-suppressor p53 is a transcription factor that regulates a number of genes in the process of cell-cycle inhibition, apoptosis, and DNA damage. Recent studies have revealed a crucial role for p53 in bone remodeling. In our previous studies we have shown that p53 is an important regulator of osteoblast differen...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-011-9533-x

    authors: Chen H,Hays E,Liboon J,Neely C,Kolman K,Chandar N

    更新日期:2011-11-01 00:00:00

  • The essential role of fetuin in the serum-induced calcification of collagen.

    abstract::The mineral in bone is located primarily within the collagen fibril, and during mineralization the fibril is formed first and then water within the fibril is replaced with mineral. Our goal is to understand the mechanism of fibril mineralization, and as a first step we recently determined the size exclusion characteri...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-007-9085-2

    authors: Toroian D,Price PA

    更新日期:2008-02-01 00:00:00

  • Virally induced periosteal osteogenesis in mice.

    abstract::Inoculation of Moloney murine sarcoma virus (M-MSV) into shank muscles of adult NMRI mice resulted in localized sarcoma and periosteal membrane proliferation with subsequent periosteal bone formation. Newly formed bone arose from outer surface of shank bones. Two weeks after M-MSV inoculation the width of bone cortex ...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/BF02409426

    authors: Włodarski K,Kobus M,Luczak M,Dołowy J

    更新日期:1981-01-01 00:00:00

  • Risedronate increases bone density and reduces vertebral fracture risk within one year in men on corticosteroid therapy.

    abstract::Limited information is available on the effect of bisphosphonates in men receiving corticosteroid therapy. We studied 184 men among the patients enrolled in two, double-blind, placebo-controlled, 1-year studies with similar protocols. The studies evaluated the effects of risedronate in patients beginning corticosteroi...

    journal_title:Calcified tissue international

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1007/s00223-001-1060-8

    authors: Reid DM,Adami S,Devogelaer JP,Chines AA

    更新日期:2001-10-01 00:00:00

  • Effects of SP500263, a novel selective estrogen receptor modulator, on bone, uterus, and serum cholesterol in the ovariectomized rat.

    abstract::We describe here the activity of a novel selective estrogen receptor modulator, SP500263. When given to adult ovariectomized (OVX) rats for 28 days at doses of 0.3, 1, or 3 mg/kg/day, we found that SP500263 partially protected against OVX-induced loss of bone mineral content in the distal ends of femurs and in the who...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-002-1029-2

    authors: Sutherland MK,Brady H,Gayo-Fung LM,Leisten J,Lipps SG,McKie JA,O'Leary E,Patnaik N,Anderson DW,Bhagwat SS,Stein B

    更新日期:2003-06-01 00:00:00

  • Epidemiological survey of ossification of the posterior longitudinal ligament in an adult Korean population: three-dimensional computed tomographic observation of 3,240 cases.

    abstract::The prevalence rates of ossification of the posterior longitudinal ligament (OPLL) in the Korean population were reported as 3.4 and 0.6%. However, these studies were performed before the era of three-dimensional computed tomography (3D CT). We investigated the prevalence of cervical OPLL on the basis of 3D CT and fea...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-014-9846-7

    authors: Sohn S,Chung CK,Yun TJ,Sohn CH

    更新日期:2014-06-01 00:00:00

  • Rapid establishment of chemical and mechanical properties during lamellar bone formation.

    abstract::The development of prophylaxes and treatments of bone diseases that can effectively increase the strength of bone as a structure necessitates a better understanding of the time course by which chemical properties define the stiffness of the material during primary and secondary mineralization. It was hypothesized that...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-005-0148-y

    authors: Busa B,Miller LM,Rubin CT,Qin YX,Judex S

    更新日期:2005-12-01 00:00:00

  • 1,25 Dihydroxyvitamin D3 modifies cyclosporine-induced bone loss.

    abstract::We have previously shown that cyclosporin A (CsA) produces high bone remodeling with resorption exceeding formation and loss of bone volume in the rat. This may have important clinical implications where CsA is widely used in organ transplantation. 1,25 dihydroxyvitamin D3 (1,25(OH)2D3) is a bone mineralizing hormone ...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/BF02555980

    authors: Epstein S,Schlosberg M,Fallon M,Thomas S,Movsowitz C,Ismail F

    更新日期:1990-09-01 00:00:00

  • Osteonecrosis in a chemically induced rat model of human hemolytic disorders associated with thrombosis--a new model for avascular necrosis of bone.

    abstract::Bone injury occurs in human hemolytic disorders associated with thrombosis, such as beta-thalassemia and sickle cell disease. Exposure of rats to 2-butoxyethanol (BE) has been associated with hemolytic anemia, disseminated thrombosis, and infarction in multiple organs including bone. This rat model apparently mimics a...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-003-0068-7

    authors: Shabat S,Nyska A,Long PH,Goelman G,Abramovitch R,Ezov N,Levin-Harrus T,Peddada S,Redlich M,Yedgar S,Nyska M

    更新日期:2004-03-01 00:00:00