Abstract：
:The mineral in bone is located primarily within the collagen fibril, and during mineralization the fibril is formed first and then water within the fibril is replaced with mineral. Our goal is to understand the mechanism of fibril mineralization, and as a first step we recently determined the size exclusion characteristics of the fibril. This study indicates that apatite crystals up to 12 unit cells in size can access the water within the fibril while molecules larger than a 40-kDa protein are excluded. We proposed a novel mechanism for fibril mineralization based on these observations, one that relies exclusively on agents excluded from the fibril. One agent generates crystals outside the fibril, some of which diffuse into the fibril and grow, and the other selectively inhibits crystal growth outside of the fibril. We have tested this mechanism by examining the impact of removing the major serum inhibitor of apatite growth, fetuin, on the serum-induced calcification of collagen. The results of this test show that fetuin determines the location of serum-driven mineralization: in fetuin's presence, mineral forms only within collagen fibrils; in fetuin's absence, mineral forms only in solution outside the fibrils. The X-ray diffraction spectrum of serum-induced mineral is comparable to the spectrum of bone crystals. These observations show that serum calcification activity consists of an as yet unidentified agent that generates crystal nuclei, some of which diffuse into the fibril, and fetuin, which favors fibril mineralization by selectively inhibiting the growth of crystals outside the fibril.
journal_name
Calcif Tissue Intjournal_title
Calcified tissue internationalauthors
Toroian D,Price PAdoi
10.1007/s00223-007-9085-2subject
Has Abstractpub_date
2008-02-01 00:00:00pages
116-26issue
2eissn
0171-967Xissn
1432-0827journal_volume
82pub_type
杂志文章abstract：:The effects of long-term running were studied in 20 beagle dogs. Ten dogs ran from the age of 15 weeks to the age of 70 weeks in a progressive program for up to 40 km/day. Ten sister dogs spent the same time in individual cages. At the age of 70 weeks the spines of the dogs were studied with regard to mineral density ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02555099
更新日期：1991-01-01 00:00:00
abstract：:Regular tennis play is associated with impressive asymmetries in bone strength in favor of the racquet arm, particularly in the humerus. However, the relative effects of service and ground strokes are not known. Serendipitously, we encountered a 46-year-old regular tennis player who has played service and ground strok...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-015-9995-3
更新日期：2015-08-01 00:00:00
abstract：:A comparative polarized light (PLM), scanning (SEM), and transmission (TEM) electron microscopy study was carried out on cross- and longitudinal sections of human lamellar bone in the tibiae of four male subjects aged 9, 23, 45, and 70 years. SEM analysis was also performed on rectangular-prismatic samples in order to...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF01673402
更新日期：1993-01-01 00:00:00
abstract：:Polymorphisms in the region of the gene for the vitamin D receptor (VDR) (chromosome 12q12-14) have been associated with differences in bone mineral density (BMD) in some studies but not in others. Because linkage analysis assesses allele sharing identical-by-descent among relatives instead of the association of a par...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s002230001175
更新日期：2000-12-01 00:00:00
abstract：:The main congenital conditions of hypophosphatemia expressed in adulthood include several forms of hereditary hypophosphatemic rickets and a congenital disorder of vitamin D metabolism characterized by osteomalacia and hypophosphatemia in adult patients. Hypophosphatemia in adults is defined as serum phosphate concent...
journal_title：Calcified tissue international
pub_type： 杂志文章,评审
doi：10.1007/s00223-020-00695-2
更新日期：2021-01-01 00:00:00
abstract：:Extracellular ATP, and to a lesser extent adenosine, an ATP metabolite, stimulated cell proliferation in osteoblast-like cells (MC3T3-E1). ATP increased cytosolic Ca2+ due to Ca2+ mobilization from intracellular storage in the same concentration range of the nucleotide as that effective for DNA synthesis, suggesting t...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02529732
更新日期：1996-02-01 00:00:00
abstract：:We have previously noted that a relatively large load (150 N) is required to induce a strain on the cortex of rat vertebrae similar to that induced on weight-bearing bones by normal mechanical usage. It seems unlikely that the musculature of the tail normally imposes loads of this magnitude, and this suggests that the...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s002239900097
更新日期：1996-08-01 00:00:00
abstract：:Twenty-five female Caucasians, aged 19-57 years, with the hypermobility syndrome had bone density measurements using established noninvasive techniques such as dual X-ray absorptiometry (DXA), single photon absorptiometry (SPA), heel ultrasound (US), and peripheral computed tomography (pQCT) acquisitions of the radius...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223001093
更新日期：2000-07-01 00:00:00
abstract：:The protein components of biomineralized structures (matrix proteins) are believed to modulate crystal nucleation and growth, and thereby influence the shape and strength of the final structure. The chicken eggshell contains a complex array of distinct matrix proteins. The most abundant of these was purified to homoge...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF00298593
更新日期：1995-06-01 00:00:00
abstract：:The discriminating ability and relevance of clinical risk factors, quantitative ultrasound (QUS) variables, X-ray-based bone mineral density (BMD) and hip axis length (HAL) measurements to evaluate the risk of osteoporotic fracture in elderly Brazilian women were examined in this study. QUS at the calcaneus (Achilles ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-002-1096-4
更新日期：2003-12-01 00:00:00
abstract：:We studied the influence of age, gender, latitude, season, diet and ethnicity on plasma 25-hydroxyvitamin D 25 OHD, PTH, 1,25-dihydroxyvitamin D, vitamin D-binding protein, bone-specific alkaline phosphatase, and osteocalcin levels in 46 Greenlanders living in Nuuk (64 degrees N) on a traditional fare (group A), 45 Gr...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-003-0110-9
更新日期：2004-03-01 00:00:00
abstract：:Quantitative computed tomography (QCT) was compared to dual X-ray absorptiometry (DXA) measured in the lumbar spine of 508 European women defined as normal without fracture (NoF), or osteoporotic (OP), with either vertebral fracture (VF), or peripheral fracture (PF). The correlations between QCT and DXA BMD measuremen...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02678144
更新日期：2001-02-01 00:00:00
abstract：:The method of heat-denaturation and trypsin digestion was used to dissect bone biochemically into mineral-protected and mineral-unprotected pools of collagenous matrix. It was found that varying the particle size of the bone powder had a profound effect on the results. Using mature bovine cortical bone, the observed p...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02553750
更新日期：1988-04-01 00:00:00
abstract：:Mononuclear phagocytes have been implicated as important cellular elements in the process of bone resorption. We have postulated that the recruitment and migration of mononuclear phagocytes to bone occurs via a mechanism(s) in which bone-derived chemotactic factors (BDCF) are released from foci undergoing resorption. ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02557681
更新日期：1985-01-01 00:00:00
abstract：:There is considerable interest in determining whether hypergravity can be used as a countermeasure for microgravity-induced bone loss. This study was conducted on 20 immature male rats in order to investigate possible elastic adaptations of cortical bone in rapidly growing rats exposed to chronic hypergravity. Ten rat...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s002239900111
更新日期：1996-09-01 00:00:00
abstract：:Duffy antigen receptor for chemokines (DARC) binds to a number of pro-inflammatory chemokines, and since chemokines are known to regulate trafficking of osteoclast (OC) precursors, we predicted that DARC would regulate OC recruitment to sites of inflammation by modulating chemokine activity. To test this hypothesis, w...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-016-0170-2
更新日期：2016-11-01 00:00:00
abstract：:We have compared two components of bacterial cell walls, muramyl dipeptide (MDP) and lipopolysaccharide (LPS), for their effects on bone resorption as measured by the release of previously incorporated 45Ca. MDP is the smallest active component of peptidoglycan, whereas LPS is the active component of endotoxin. Fetal ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02411269
更新日期：1982-07-01 00:00:00
abstract：:At 4 and 24 hours after injection of (35S)sulfate, there were 22-67% fewer silver grains on conventionally fixed sections than on sections fixed by cryotechniques. Differences were smaller when (3H) glucosamine was chosen as precursor. The number of silver grains increased between 4 and 24 hours in predentine and in a...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02557834
更新日期：1985-09-01 00:00:00
abstract：:High PTH levels increase bone turnover and decrease bone mineral density (BMD). Low plasma 25-hydroxyvitamin D (25OHD) levels cause secondary hyperparathyroidism, but the relative contribution of low 25OHD and high PTH levels on risk of fracture is largely unknown. Within the cohort of women (n = 2,016) included in th...
journal_title：Calcified tissue international
pub_type： 杂志文章,多中心研究
doi：10.1007/s00223-010-9454-0
更新日期：2011-03-01 00:00:00
abstract：:The acute-phase response (APR) is frequently observed in patients treated with intravenous (iv) zoledronate (ZOL). We investigated whether a short course of rosuvastatin (ROSU) could attenuate the ZOL--induced APR through blocking the mevalonate pathway at a proximal level. Twenty-eight osteoporotic postmenopausal wom...
journal_title：Calcified tissue international
pub_type： 杂志文章,随机对照试验
doi：10.1007/s00223-011-9468-2
更新日期：2011-05-01 00:00:00
abstract：:The influence of body mass index (BMI) on T scores for total body bone mineral content (TBBMC) and regional bone mineral content (RBMC) was studied in 186 healthy women: 100 postmenopausal, 35 perimenopausal, and 51 premenopausal. The three groups were divided by BMI >25 kg/m2 and BMI <25 kg/m2 and the postmenopausal ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s002239900310
更新日期：1997-08-01 00:00:00
abstract：:Vitamin D supplements are often recommended to restore sufficiency, although the adherence to treatment is low. This study assessed vitamin D status at different time intervals following the cessation of treatment. The database of Clalit-Health-Services (CHS), a not-for-profit HMO covering more than half of the Israel...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-011-9536-7
更新日期：2011-11-01 00:00:00
abstract：:The type IA osteogenesis imperfecta (OI) phenotype is characterized by multiple fractures, blue sclerae, and minimal skeletal deformity without dentinogenesis imperfecta. The object of this study was to determine the effect of treatment with intravenous pamidronate (30 mg) every 3 months on bone density and bone histo...
journal_title：Calcified tissue international
pub_type： 临床试验,杂志文章
doi：10.1007/s00223-001-1055-5
更新日期：2003-02-01 00:00:00
abstract：:The aim of this study was to investigate the effect of transplanted chondrocytes on endochondral bone formation in cartilage repair tissue. In the knee joint of miniature pigs, cartilage lesions were treated by microfracturing and were then either left empty, covered with a collagen membrane, or treated by matrix-asso...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-009-9288-9
更新日期：2009-11-01 00:00:00
abstract：:Limited information is available on the effect of bisphosphonates in men receiving corticosteroid therapy. We studied 184 men among the patients enrolled in two, double-blind, placebo-controlled, 1-year studies with similar protocols. The studies evaluated the effects of risedronate in patients beginning corticosteroi...
journal_title：Calcified tissue international
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1007/s00223-001-1060-8
更新日期：2001-10-01 00:00:00
abstract：:Men and women (n = 518) receiving moderate-to-high doses of corticosteroids were enrolled in two studies with similar protocols and randomly assigned to receive either placebo or risedronate (2.5 or 5 mg) for 1 year. All patients received daily calcium supplementation (500-1000 mg), and most also received supplemental...
journal_title：Calcified tissue international
pub_type： 临床试验,杂志文章,多中心研究,随机对照试验
doi：10.1007/s002230001146
更新日期：2000-10-01 00:00:00
abstract：:The aim of this study was to follow the changes in bone mineral density (BMD) and biochemical markers of bone turnover in 10 children (7.5-17.5 years of age) with severe juvenile idiopathic arthritis (JIA), during a 3-year therapy with salmon calcitonin (100 IU/day 2 months on and 2 off for a year and 200 IU/day for 2...
journal_title：Calcified tissue international
pub_type： 临床试验,杂志文章
doi：10.1007/s00223-001-0008-3
更新日期：2001-07-01 00:00:00
abstract：:In vitro models of osteogenesis are essential for investigating bone biology and the effects of pharmaceutical, chemical, and physical cues on bone formation. Osteogenesis takes place in a complex three-dimensional (3D) environment with cells from both mesenchymal and hematopoietic origins. Existing in vitro models of...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-011-9467-3
更新日期：2011-05-01 00:00:00
abstract：:Several rare inherited disorders have been described that show phenotypic overlap with Paget's disease of bone (PDB) and in which PDB is a component of a multisystem disorder affecting muscle and the central nervous system. These conditions are the subject of this review article. Insertion mutations within exon 1 of t...
journal_title：Calcified tissue international
pub_type： 杂志文章,评审
doi：10.1007/s00223-019-00520-5
更新日期：2019-05-01 00:00:00
abstract：:Impact activity is an important contributor to bone accrual. Children engaging in such activity have been shown to have greater bone mineral density (BMD) than their peers. This cross-sectional study was designed to quantify the association between BMD and impact activity in pre-pubescent girls, specifically examining...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-001-1131-x
更新日期：2003-01-01 00:00:00
