Ultrastructure of cultured marginal cells of the guinea pig cochlea.

Abstract:

:Explants of stria vascularis and spiral ligament of guinea pig cochlea were kept in primary culture. On the explant, proliferating marginal cells advanced by 15 microns/day, suggesting that in vivo defects of the strial epithelium can be covered by new marginal cells. The marginal cells growing in the cell culture dish had a diameter of 12.8 +/- 0.7 microns and formed an epithelial monolayer. Adjacent cells were connected by desmosomes and tight junctions. The cells were uniformly polarized. The apical membrane had small invaginations and numerous microvillus-like extensions, and the convoluted lateral membrane interdigitated with adjacent cells. The basal infoldings were smaller in cultured cells than in vivo; mitochondria were dispersed in the entire cytoplasm rather than concentrated in basolateral infoldings. The basal membrane infoldings of cultured marginal cells did not interdigitate with underlying fibroblast-like cells. Marginal cells were separated from underlying fibroblast-like cells by a fluid-filled space which was sometimes enlarged, leading to the formation of "domes" in the otherwise planar epithelium.

journal_name

Acta Otolaryngol

journal_title

Acta oto-laryngologica

authors

Melichar I,Gitter AH

doi

10.3109/00016489209137471

subject

Has Abstract

pub_date

1992-09-01 00:00:00

pages

762-6

issue

5

eissn

0001-6489

issn

1651-2251

journal_volume

112

pub_type

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