Abstract:
:Incorporation of N-succinimidyl-3(4-hydroxyphenyl) propionate (Bolton-Hunter reagent) or its 125I-labeled derivative into peptides can be selectively directed towards either alpha- or epsilon-amine functions by modifying the pH of the reaction. Acylation of alpha-amino groups is favored at pH 6.5 whereas epsilon-amino groups react more readily at pH 8.5. We have taken advantage of this result to prepare two new 125I-labeled analogues of substance P and neurotensin that bind selectively and reversibly to their respective receptors. The method described here is of general interest and can be used to incorporate various reporter groups into peptide structures.
journal_name
Peptidesjournal_title
Peptidesauthors
Gaudriault G,Vincent JPdoi
10.1016/0196-9781(92)90027-zsubject
Has Abstractpub_date
1992-11-01 00:00:00pages
1187-92issue
6eissn
0196-9781issn
1873-5169pii
0196-9781(92)90027-Zjournal_volume
13pub_type
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