Administration of chlordiazepoxide affects [3H][3-MeHis2]thyrotropin-releasing hormone binding in rat brain.


:The effects of acute and subchronic administration of chlordiazepoxide (CDZ) on [3H][3-methyl-histidyl2]thyrotropin-releasing hormone binding to thyrotropin-releasing hormone (TRH) receptors in membrane preparations from various regions of rat brain were examined. Acute administration of CDZ (50 mg/kg x 3 within 24 h) did not alter either the equilibrium dissociation constant (Kd) or the maximum number of binding sites (Bmax) in cerebellum (CB), olfactory bulbs (OB), frontal cortex (Cx), hypothalamus (HT) or corpus striatum (ST). However, the Kds of the pyriform cortex/amygdala (PC/A) (Kd = 3.6 +/- 0.1 nM compared to 1.9 +/- 0.1 nM in the control group; p less than 0.01) and the hippocampus (HP) (Kd = 7.8 +/- 0.7 nM compared to 2.1 +/- 0.1 nM in the control group; p less than 0.01) were increased. There were no changes in Bmax. Subchronic administration of CDZ (50 mg/kg/day for 7 days) increased the Kd of the PC/A complex (p less than 0.05), the OB (p less than 0.05) and the HP (p less than 0.01) without altering in Bmax. These results, showing regional differences in the response of TRH receptors to acute and subchronic CDZ administration, suggest that reduced affinity of TRH receptors in the PC/A complex, OB and HP may be related to some of the neurobiological actions of CDZ and/or its metabolites.






Shimoda K,Mason GA,Walker CH,Garbutt JC,Prange AJ Jr




Has Abstract


1991-01-01 00:00:00
















  • Further structure-activity studies of lactam derivatives of MT-II and SHU-9119: their activity and selectivity at human melanocortin receptors 3, 4, and 5.

    abstract::Recently we have demonstrated that replacing His(6) by constrained amino acids(2) in the well-known antagonist SHU-9119 resulted in potent and selective antagonist ligands especially at the hMC3R and hMC5 receptors. With the aim to further explore position 6 in the sequence of SHU-9119 and MT-II, we have designed, syn...


    pub_type: 杂志文章


    authors: Grieco P,Cai M,Han G,Trivedi D,Campiglia P,Novellino E,Hruby VJ

    更新日期:2007-06-01 00:00:00

  • Ontogeny and molecular weight of immunoreactive arginine vasopressin and corticotropin-releasing factor in the ovine fetal hypothalamus.

    abstract::In sheep, arginine vasopressin (AVP) and corticotropin-releasing factor (CRF) stimulate adrenocorticotropic hormone (ACTH) secretion. Increased hypothalamic secretion of these peptides at the end of gestation initiates parturition in this species. We hypothesized that CRF and/or AVP content in the fetal hypothalamus i...


    pub_type: 杂志文章


    authors: Saoud CJ,Wood CE

    更新日期:1996-01-01 00:00:00

  • Intracerebroventricular angiotensin II increases intraoral intake of water in rats.

    abstract::Ad lib and intraoral intake tests can separate the effects of drugs on the appetitive and consummatory phases of ingestive behavior. Central angiotensin II increases ad lib intake from water bottles, but its effect on intraoral intake has not been examined. Rats with both lateral intracerebroventricular (i.c.v.) cannu...


    pub_type: 杂志文章


    authors: Houpt TA,Corp ES,Berlin R

    更新日期:1998-01-01 00:00:00

  • In vitro interaction of ACTH with rat brain muscarinic receptors.

    abstract::ACTH-(1-24) inhibits the in vitro binding of the muscarinic antagonist [3H]QNB to membranes from rat brain. The magnitude of inhibition is dependent on the concentration of ACTH-(1-24). Kinetic analysis indicates a pure competitive inhibition which is suggestive of a reversible interaction of ACTH with muscarinic rece...


    pub_type: 杂志文章


    authors: Tonnaer JA,Van Vugt M,De Graaf JS

    更新日期:1986-05-01 00:00:00

  • Effect of epidermal growth factor (EGF) on [3H]TdR incorporation into DNA in ad lib fed and fasted CD2F1 mice.

    abstract::The effect of EGF on the incorporation of [3H]TdR into DNA (DNA synthesis) was determined in the esophagus, liver, pancreas, and kidney in mice standardized to 12 hours (hr) of light alternating with 12 hr of darkness. A question asked was whether intraperitoneally administered EGF could alter the circadian patterns o...


    pub_type: 杂志文章


    authors: Scheving LA,Tsai TH,Scheving LE,Hoke WS

    更新日期:1987-03-01 00:00:00

  • Rapid presymptomatic detection of PrPSc via conformationally responsive palindromic PrP peptides.

    abstract::Structurally unique, synthetic prion peptides provide the basis of a simple assay to serve as both a detection and signal amplification system that distinguishes the normal prion protein, PrPC, from the misfolded prion protein, PrPSc, that is associated with the occurrence of transmissible spongiform encephalopathies ...


    pub_type: 杂志文章


    authors: Grosset A,Moskowitz K,Nelsen C,Pan T,Davidson E,Orser CS

    更新日期:2005-11-01 00:00:00

  • Upregulation of ANP and NPR-C mRNA in the kidney and heart of eNOS knockout mice.

    abstract:OBJECTIVES:The aim of the present studywas to examine the question of whether the atrial natriuretic peptide (ANP) system is altered by endothelial nitric-oxide synthase (eNOS). METHODS:Male eNOS-deficient mice (eNOS-/-) and wild type control mice (eNOS+/+, C57B1/6J) were used. Blood pressure was measured in anestheti...


    pub_type: 杂志文章


    authors: Yuan K,Kim SY,Oh YB,Yu J,Shah A,Park BH,Kim SH

    更新日期:2010-07-01 00:00:00

  • Metabolic fate of nociceptin/orphanin FQ in the rat spinal cord and biological activity of its released fragment.

    abstract::Metabolism of orphanin FQ/nociceptin (OFQ/N) was studied in the spinal cord of rats. The heptadecapeptide was efficiently cleaved by a neutral serine endopeptidase, thus releasing the major metabolite, OFQ/N(1-11), further truncated to the final product, OFQ/N(1-6). Biologic activity of this latter fragment was tested...


    pub_type: 杂志文章


    authors: Suder P,Kotlinska J,Smoluch MT,Sällberg M,Silberring J

    更新日期:1999-01-01 00:00:00

  • Bovine pancreatic polypeptide-like immunoreactivity in brain and peripheral nervous system: coexistence with catecholaminergic nerves.

    abstract::The rat central and peripheral nervous system contains a widespread distribution of BPP-like immunoreactive neurons. Some of these neurons coexist with a catecholamine, probably mostly NE. This peptide appears to be releaseable by nerve stimulation. Catecholamine releasing agents such as reserpine do not appear to dep...


    pub_type: 杂志文章


    authors: Jacobowitz DM,Olschowka JA

    更新日期:1982-05-01 00:00:00

  • Central LIF gene therapy suppresses food intake, body weight, serum leptin and insulin for extended periods.

    abstract::Leukemia inhibitory factor (LIF) overexpression, induced by the intracerebroventricular (i.c.v.) injection of an recombinant adeno-associated viral vector encoding LIF (rAAV-LIF), resulted in a dose-dependent reduction in body weight (BW) gain, food intake (FI) and adiposity, evidenced by suppression of serum leptin a...


    pub_type: 杂志文章


    authors: Beretta E,Dhillon H,Kalra PS,Kalra SP

    更新日期:2002-05-01 00:00:00

  • Corticotropin-releasing factor from the rat brain measured by protein immunoblot.

    abstract::The ability to measure changes in brain levels of corticotropin-releasing factor (CRF) is an important step toward understanding the role of this neuropeptide in mood states. Here, we report for the first time that the protein (Western) immunoblot assay can be used to detect and quantify CRF (4.7 kDa) from the rat bra...


    pub_type: 杂志文章


    authors: Meloni EG,Jackson AV,Cohen BM,Carlezon WA Jr

    更新日期:2005-11-01 00:00:00

  • Distribution of pancreatic polypeptide and peptide YY.

    abstract::The cellular distribution of PP and PYY in mammals is reviewed. Expression of PP is restricted to endocrine cells mainly present in the pancreas predominantly in the duodenal portion (head) but also found in small numbers in the gastro-intestinal tract. PYY has a dual expression in both endocrine cells and neurons. PY...


    pub_type: 杂志文章,评审


    authors: Ekblad E,Sundler F

    更新日期:2002-02-01 00:00:00

  • Neuronal messengers in the human cerebral circulation.

    abstract::In recent years our knowledge of the nervous control of the cerebral circulation has increased. The use of denervations and retrograde tracing in combination with immunohistochemical techniques has demonstrated that cerebral vessels are supplied with sympathetic, parasympathetic, and sensory nerve fibers and possibly ...


    pub_type: 杂志文章,评审


    authors: Gulbenkian S,Uddman R,Edvinsson L

    更新日期:2001-06-01 00:00:00

  • Met-enkephalin concentrations in striatum respond reciprocally to alterations in dopamine neurotransmission.

    abstract::The striatum is richly innervated by both enkephalinergic and dopaminergic neurons, providing an anatomic framework from which intimate functional interrelationships between these neuronal systems may be postulated. Accordingly, many functional processes within dopamine neurons have been shown to be modulated by opioi...


    pub_type: 杂志文章


    authors: George SR,Kertesz M

    更新日期:1987-05-01 00:00:00

  • Anxiolytic effect of motilin and reversal with GM-109, a motilin antagonist, in mice.

    abstract::There have been few reports on the effects of the brain-gut peptide motilin on the central nervous system (CNS). We administered motilin intracerebroventricularly to mice and investigated the effect of motilin on anxiety using an elevated plus-maze. Motilin produced a significant decrease in anxiety with an inverted U...


    pub_type: 杂志文章


    authors: Momose K,Inui A,Asakawa A,Ueno N,Nakajima M,Kasuga M

    更新日期:1998-01-01 00:00:00

  • Structural characterization of calcitonin gene-related peptide purified from rabbit intestine.

    abstract::Calcitonin gene-related peptide (CGRP) immunoreactive material has been found in extracts of the intestine, however, the structure of intestinal CGRP is not known. Analytical reverse phase HPLC and ion-exchange FPLC revealed one predominant immunoreactive CGRP peak in rabbit intestinal extracts. This material was puri...


    pub_type: 杂志文章


    authors: Eysselein VE,Reeve JR Jr,Sternini C,Cominelli F,Davis WM,Davis MT,Lee TD,Ho FJ,Ridout D,Shively JE

    更新日期:1991-03-01 00:00:00

  • Isolation and characterization of a novel mung bean protease inhibitor with antipathogenic and anti-proliferative activities.

    abstract::A novel protease inhibitor, designated mungoin, with both antifungal and antibacterial activities, and exhibiting a molecular mass of 10kDa in SDS-polyacrylamide gel electrophoresis, was isolated from mung bean (Phaseolus mungo) seeds. The isolation procedure involved a combination of extraction, ammonium sulfate prec...


    pub_type: 杂志文章


    authors: Wang S,Lin J,Ye M,Ng TB,Rao P,Ye X

    更新日期:2006-12-01 00:00:00

  • Isolation and characterization of a variant form of vasoactive intestinal polypeptide.

    abstract::A variant form of the vasoactive intestinal polypeptide (VIP) has been isolated. It was found to consist of a molecule which instead of the C-terminal asparagine amide of VIP has a C-terminal extension of Gly-Lys-Arg. This VIP variant displaces VIP in a VIP receptor assay, reacts with N-terminally-directed antisera in...


    pub_type: 杂志文章


    authors: Gafvelin G,Andersson M,Dimaline R,Jörnvall H,Mutt V

    更新日期:1988-05-01 00:00:00

  • The development of the MCH system.

    abstract::Although a great deal is published on the MCH neurons, very few works were devoted to the study of their development. However, existing literature points out two important traits: first, these neurons differentiate a MCH phenotype very early in all species studied so far, which might suggest a role for the MCH peptide...


    pub_type: 杂志文章,评审


    authors: Risold PY,Croizier S,Legagneux K,Brischoux F,Fellmann D,Griffond B

    更新日期:2009-11-01 00:00:00

  • Correlation between the expression of the histone H4 mRNA variant H4-v.1 and the levels of histone H4-(86-100) and H4-(89-102) (OGP) in various rat tissues and alveolar macrophages.

    abstract::We studied the expression of the osteogenic and antinociceptive C-terminal histone H4-related peptide fragments, H4-(89-102) (OGP) and H4-(86-100), respectively, within various rat tissues and isolated alveolar macrophages (AM) by radioimmunoassay (RIA). OGP was located mainly within the bone marrow, spleen, thymus, a...


    pub_type: 杂志文章


    authors: Poirier R,Lemaire I,Dumont M,Leduc N,Le HT,Lemaire S

    更新日期:2005-08-01 00:00:00

  • Animals models of MCH function and what they can tell us about its role in energy balance.

    abstract::Melanin-concentrating hormone (MCH) has attracted considerable attention because of its effects on food intake and body weight and the MCH receptor (MCHR1) remains one of the viable targets for obesity therapy. This review summarizes the literature examining the effects of MCH on body weight, food intake and energy ex...


    pub_type: 杂志文章,评审


    authors: Pissios P

    更新日期:2009-11-01 00:00:00

  • Local renin-angiotensin systems in the adrenal gland.

    abstract::In the adrenal gland all components of the renin-angiotensin system (RAS) are expressed in both the adrenal cortex and the adrenal medulla. In this review evidence shall be presented that a local secretory RAS exists in the adrenal cortex that stimulates aldosterone production and serves as an amplification system for...


    pub_type: 杂志文章,评审


    authors: Peters J

    更新日期:2012-04-01 00:00:00

  • Differential antagonism of endomorphin-1 and endomorphin-2 supraspinal antinociception by naloxonazine and 3-methylnaltrexone.

    abstract::To determine if different subtypes of mu-opioid receptors were involved in antinociception induced by endomorphin-1 and endomorphin-2, the effect of pretreatment with various mu-opioid receptor antagonists beta-funaltrexamine, naloxonazine and 3-methylnaltrexone on the inhibition of the paw-withdrawal induced by endom...


    pub_type: 杂志文章


    authors: Sakurada S,Hayashi T,Yuhki M,Fujimura T,Murayama K,Yonezawa A,Sakurada C,Takeshita M,Sato T,Zadina JE,Kastin AJ,Sakurada T

    更新日期:2002-05-01 00:00:00

  • Identification of a novel male reproduction-related gene and its regulated expression patterns in the prawn, Macrobrachium rosenbergii.

    abstract::To identify male-specific genes that could be involved in male development, we screened a subtracted male reproductive tract library and isolated a novel gene named Mar-Mrr (M. rosenbergii male reproduction-related gene). The Mar-Mrr cDNA sequence consists of 683 nucleotides with a 333 nucleotide open reading frame, e...


    pub_type: 杂志文章


    authors: Cao JX,Yin GL,Yang WJ

    更新日期:2006-04-01 00:00:00

  • Differential effects of amylin on memory processing using peripheral and central routes of administration.

    abstract::Amylin is a peptide hormone secreted from the beta cells of the pancreatic islets. Amylin was administered peripherally or centrally following weak or strong training on footshock avoidance conditioning in a T-maze. Under conditions of weak training, amylin improved memory retention in a dose-dependent manner. Under c...


    pub_type: 杂志文章


    authors: Flood JF,Morley JE

    更新日期:1992-05-01 00:00:00

  • Functional roles of the NTS2 and NTS3 receptors.

    abstract::Neurotensin exerts its actions in the central nervous system and the periphery through three identified receptors. Two of them, the NTS2 and NTS3, display unusual properties either because of their complex signal transduction mechanisms (NTS2) or because of their structural composition as a non-G-protein-coupled recep...


    pub_type: 杂志文章,评审


    authors: Mazella J,Vincent JP

    更新日期:2006-10-01 00:00:00

  • GLP-1 receptor agonists show neuroprotective effects in animal models of diabetes.

    abstract::Enzyme-resistant receptor agonists of the incretin hormone glucagon-like peptide-1 (GLP-1) have shown positive therapeutic effects in people with type 2 diabetes mellitus (T2DM). T2DM has detrimental effects on brain function and impairment of cognition and memory formation has been described. One of the underlying me...


    pub_type: 杂志文章,评审


    authors: Gault VA,Hölscher C

    更新日期:2018-02-01 00:00:00

  • Validity of multiple-time regression analysis in measurement of tritiated and iodinated leptin crossing the blood-brain barrier: meaningful controls.

    abstract::Multiple-time regression analysis has been used to study the influx of radiolabeled peptides and polypeptides across the blood-brain barrier (BBB). This study used both tritiated and iodinated leptin to clarify several issues associated with these measurements. Recombinant murine leptin was radiolabeled with 3H by der...


    pub_type: 杂志文章


    authors: Kastin AJ,Akerstrom V,Pan W

    更新日期:2001-12-01 00:00:00

  • Intracerebroventricular administration of neuronostatin delays gastric emptying and gastrointestinal transit in mice.

    abstract::Neuronostatin is a 13-amino acid amidated peptide widely distributed in various organs including gastrointestinal tract. However, the effect of neuronostatin on gastrointestinal motility has not been well characterized. In the present work, effects of central administration of neuronostatin on gastric emptying and gas...


    pub_type: 杂志文章


    authors: Su SF,Yang AM,Yang SB,Wang NB,Lu SS,Wang HH,Chen Q

    更新日期:2012-05-01 00:00:00

  • Structure-activity relationship of human liver-expressed antimicrobial peptide 2.

    abstract::Liver-expressed antimicrobial peptide 2 (LEAP-2) is a 40-residue cationic peptide originally purified from human blood ultrafiltrate. The native peptide contains two disulfide bonds and is unique regarding its primary structure. Its biological role is not known but a previous study showed that chemically synthesized L...


    pub_type: 杂志文章


    authors: Hocquellet A,Odaert B,Cabanne C,Noubhani A,Dieryck W,Joucla G,Le Senechal C,Milenkov M,Chaignepain S,Schmitter JM,Claverol S,Santarelli X,Dufourc EJ,Bonneu M,Garbay B,Costaglioli P

    更新日期:2010-01-01 00:00:00