当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Role of Wnt10b and C/EBPalpha in spontaneous adipogenesis of 243 cells.

Role of Wnt10b and C/EBPalpha in spontaneous adipogenesis of 243 cells.

Abstract：

:This report examines the balance of positive and negative adipogenic factors in a line of immortalized 243 embryonic fibroblasts that undergo spontaneous preadipocyte differentiation. Control of adipogenesis reflects the interplay of factors that promote or inhibit expression of C/EBPalpha and PPARgamma. The 243 cells express C/EBPalpha early and at elevated levels compared to 3T3-F442A preadipocytes or adipocytes. Cell clones were derived from the heterogeneous 243 population for ability or inability to differentiate into adipocytes. Wnt10b, a secreted protein that inhibits adipogenesis, is expressed at high levels in cells with low adipogenic potential and is undetectable in preadipocytes that spontaneously differentiate. In contrast, C/EBPalpha is expressed at reduced levels in cells with low adipogenic potential, and is expressed at high levels in preadipocytes that spontaneously differentiate. These data are consistent with a model in which decreased Wnt10b, coupled with increased C/EBPalpha, results in induction of PPARgamma and spontaneous adipogenesis of 243 cells.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Bennett CN,Hodge CL,MacDougald OA,Schwartz J

doi

10.1016/s0006-291x(03)00092-5

subject

Has Abstract

pub_date

2003-02-28 00:00:00

pages

12-6

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006291X03000925

journal_volume

302

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Illegitimate recombination in a bovine papillomavirus shuttle vector: a high level of site specificity.

    abstract：:Recombination in a bovine papillomavirus shuttle vector carrying direct repeats of Moloney murine leukemia virus LTR sequence was examined. Differently from similar vectors carrying direct repeats of SV40 polyA addition signal or neomycin resistance gene, the vector exhibited no homologous recombination between the re...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(91)91362-g

    authors： Kitamura Y,Naito A,Yoshikura H

    更新日期：1991-08-30 00:00:00

  • Cyclosporine downregulates Fas ligand expression on vascular endothelial cells: implication for accelerated vasculopathy by immunosuppressive therapy.

    abstract：:Although the introduction of cyclosporine A (CyA) and FK506 for immunosuppressive therapy has dramatically enhanced the early survival of organ transplant recipients, administration of these immunosuppresants is correlated with high incidence of transplant arteriosclerosis. Transplant-associated arteriosclerosis is be...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1392

    authors： Sata M,Walsh K

    更新日期：1999-09-24 00:00:00

  • Why is phosphonodifluoromethyl phenylalanine a more potent inhibitory moiety than phosphonomethyl phenylalanine toward protein-tyrosine phosphatases?

    abstract：:The phosphonodifluoromethyl phenylalanine (F2Pmp) is superior to phosphonomethyl phenylalanine (Pmp) as a non-hydrolyzable phosphotyrosine (pTyr) mimetic. The difluoromethyl moiety increases the inhibitory potency of a F2Pmp-containing peptide over a Pmp-containing counterpart by 1000-fold toward the protein tyrosine ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.2716

    authors： Chen L,Wu L,Otaka A,Smyth MS,Roller PP,Burke TR Jr,den Hertog J,Zhang ZY

    更新日期：1995-11-22 00:00:00

  • Molecular characterization of human and bovine ceruloplasmin using MALDI-TOF mass spectrometry.

    abstract：:Using SDS-PAGE and MALDI-TOF mass spectrometry, we investigated the difference in the molecular structure between human and bovine ceruloplasmin. In both cases, we found that the protein is present in two majors forms of different molecular mass. The difference between human and bovine ceruloplasmin was more obvious w...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.5881

    authors： Boivin S,Aouffen M,Fournier A,Mateescu MA

    更新日期：2001-11-09 00:00:00

  • Histone deacetylase 3 (HDAC3) is recruited to target promoters by PML-RARalpha as a component of the N-CoR co-repressor complex to repress transcription in vivo.

    abstract：:PML-RARalpha is a chimeric transcription factor tightly associated with acute promyelocytic leukemia. PML-RARalpha plays an important role in the aberrant transcription repression on the target genes of wild-type retinoic acid receptors. Here, we demonstrated that HDAC3, one component of the N-CoR transcription repres...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.05.047

    authors： Atsumi A,Tomita A,Kiyoi H,Naoe T

    更新日期：2006-07-14 00:00:00

  • Identification of renin and renin messenger RNA sequence in rat ovary and uterus.

    abstract：:An increase in plasma prorenin during pregnancy suggests that prorenin might be synthesized in the ovary and the secretion of renin or prorenin may be stimulated by an ovarian steroid-mediated process. Recently, renin and angiotensinogen have been identified in human ovarian follicular fluid. However, there is conside...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(87)90466-9

    authors： Kim S,Shinjo M,Fukamizu A,Miyazaki H,Usuki S,Murakami K

    更新日期：1987-01-15 00:00:00

  • SDF-1/CXCR4 axis promotes the growth and sphere formation of hypoxic breast cancer SP cells by c-Jun/ABCG2 pathway.

    abstract：:ATP-binding cassette sub-family G member 2 (ABCG2) confers to the major phenotypes of side population (SP) cells, the cancer stem-like cells. In this study, the SP cells displayed a distinctly higher ABCG2 expression level, sphere formation efficiency (SFE) and growth rate even under hypoxia condition. CXCR4 overexpre...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.09.130

    authors： He C,Zhang H,Wang B,He J,Ge G

    更新日期：2018-10-28 00:00:00

  • Codon usage pattern in alpha 2(I) chain domain of chicken type I collagen and its implications for the secondary structure of the mRNA and the synthesis pauses of the collagen.

    abstract：:A stability map of local secondary structure of the mRNA of the triple-helical alpha 2(I) chain domain of chicken type I collagen was obtained by plotting the free energy of the optimal secondary structure of a local segment in mRNA against the segment position along a base sequence of the mRNA. It was found that the ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(90)92092-e

    authors： Zama M

    更新日期：1990-03-16 00:00:00

  • Singlet oxygen production from the peroxidase catalyzed formation of styrene glutathione adducts.

    abstract：:Recently, Stock et al. (J. Biol. Chem. 261, 15915-15922 [1986]) described a model enzyme system composed of horseradish peroxidase, hydrogen peroxide, phenol, glutathione and styrene. This system forms glutathione-styrene conjugates. Glutathione radicals and carbon-centered radicals are intermediates in this process. ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)92215-8

    authors： Kanofsky JR

    更新日期：1989-03-31 00:00:00

  • Human monoclonal antibodies produced from L-leucine methyl ester-treated and in vitro immunized peripheral blood lymphocytes.

    abstract：:Primary in vitro immunization of peripheral blood lymphocytes has been studied for the production of human monoclonal antibodies. Depletion of a lysosome-rich cell population, containing large granular lymphocytes, monocytes, and a subset of T suppressor cells, was shown to be crucial before the cells could be immuniz...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(87)80223-1

    authors： Borrebaeck CA,Danielsson L,Möller SA

    更新日期：1987-11-13 00:00:00

  • A novel function for nucleoside diphosphate kinase in Drosophila.

    abstract：:Nucleoside diphosphate (NDP) kinase is an enzyme that transfers the gamma-phosphate of nucleoside triphosphates to nucleoside diphosphates. Besides this well-defined role, recent evidence suggests that NDP kinase may be implicated in a wide variety of essential cellular processes. In this paper, we showed that the NDP...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.0158

    authors： Inoue H,Takahashi M,Oomori A,Sekiguchi M,Yoshioka T

    更新日期：1996-01-26 00:00:00

  • Antibodies reveal the cytolocalization and subunit structure of the 1,4-dihydropyridine component of the neuronal Ca2+ channel.

    abstract：:Rabbit brain synaptosomes bind the 1,4-dihydropyridine derivative (+)[3H]-PN 200-110 with an equilibrium dissociation constant of 0.04 nM and a maximal binding capacity of 400 fmol/mg of protein. Using polyclonal antibodies raised against the different components of the skeletal muscle 1,4-dihydropyridine receptor, we...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(86)80276-5

    authors： Schmid A,Barhanin J,Mourre C,Coppola T,Borsotto M,Lazdunski M

    更新日期：1986-09-30 00:00:00

  • Modulation of in vitro kidney cell growth by hepatic transfer RNAs.

    abstract：:The quiescent and proliferating status of the cell appears characterized by two different tRNA populations. The tRNA species changed both as relative percentage of total tRNA and in absolute concentration. The changes seem causally related to the proliferation status of the cell as tRNAs from proliferating hepatocytes...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.0665

    authors： Kanduc D

    更新日期：1996-04-25 00:00:00

  • Isovalerylcarnitine is a specific activator of the high calcium requiring calpain forms.

    abstract：:Isovalerylcarnitine, a product of the catabolism of L-leucine, is a potent activator of rat calpains isolated from erythrocytes, kidney, liver, skeletal and heart muscle. Only calpains II, but not calpains I, are activated by IVC, with the only exception of rat erythrocyte calpain I, the only species present in these ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(90)91775-n

    authors： Pontremoli S,Melloni E,Viotti PL,Michetti M,Di Lisa F,Siliprandi N

    更新日期：1990-02-28 00:00:00

  • Chromosomal localization of the human apoprotein CI gene and of a polymorphic apoprotein AII gene.

    abstract：:Human apoprotein(apo) CI and apo AII cDNA probes have been used to analyze the segregation of the human genes in panels of human-mouse hybrids. The apo CI (APOCI) gene segregates with chromosome 19 and the apo AII (APOA2) gene with chromosome 1. Somatic cell hybrids containing chromosome translocations were used to ma...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(84)80368-x

    authors： Knott TJ,Eddy RL,Robertson ME,Priestley LM,Scott J,Shows TB

    更新日期：1984-11-30 00:00:00

  • General order parameter based correlation analysis of protein backbone motions between experimental NMR relaxation measurements and molecular dynamics simulations.

    abstract：:Internal backbone dynamic motions are essential for different protein functions and occur on a wide range of time scales, from femtoseconds to seconds. Molecular dynamic (MD) simulations and nuclear magnetic resonance (NMR) spin relaxation measurements are valuable tools to gain access to fast (nanosecond) internal mo...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.01.018

    authors： Liu Q,Shi C,Yu L,Zhang L,Xiong Y,Tian C

    更新日期：2015-02-13 00:00:00

  • Sodium-dependent, concentrative nucleoside transport in cultured intestinal epithelial cells.

    abstract：:In a simple salts medium, monolayers of IEC-6 intestinal cells achieved concentrations of unmetabolized formycin B (an analog of inosine) about 6-fold higher than in the medium. Rates of formycin B influx were a saturable function of Na+ concentrations in the medium. Although IEC-6 cells possess sites with high affini...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(86)90738-2

    authors： Jakobs ES,Paterson AR

    更新日期：1986-11-14 00:00:00

  • Contribution of inducible nitric oxide synthase and cyclooxygenase-2 to apoptosis induction in smooth chorion trophoblast cells of human fetal membrane tissues.

    abstract：:We examined the contribution of apoptosis- and oxidative stress-associated genes to apoptosis induction in trophoblast cells of human fetal membrane tissues undergoing apoptosis during in vitro incubation. RT-PCR analyses demonstrated an increased level of HO-1, Mn-SOD, Cox-2, iNOS, TNFalpha, TNFR1, IL-1beta, IL-6, Ba...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.01.042

    authors： Yuan B,Ohyama K,Bessho T,Toyoda H

    更新日期：2006-03-17 00:00:00

  • Alpha-agglutinin expression in Saccharomyces cerevisiae.

    abstract：:A polyclonal antiserum raised against purified alpha-agglutinin was made specific for alpha-agglutinin after adsorption with a cells. The adsorbed antiserum identified alpha-agglutinin peptides on Western blots and bound to cell surface alpha-agglutinin, inhibiting the binding of alpha cells to a cells. Using the anti...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)91557-x

    authors： Wojciechowicz D,Lipke PN

    更新日期：1989-05-30 00:00:00

  • Expression of APP in brains of transgenic mice containing the entire human APP gene.

    abstract：:A major component of amyloid deposits found in Alzheimer disease and Down syndrome is the beta/A4 peptide, which is derived from the Alzheimer amyloid protein precursor (APP). Recent evidence indicates that increases in APP expression and/or beta/A4 peptide accumulation may underlie the amyloidosis characteristic of t...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.2527

    authors： Buxbaum JD,Christensen JL,Ruefli AA,Greengard P,Loring JF

    更新日期：1993-12-15 00:00:00

  • Oxidation of an organosulfur xenobiotic by microsomes from soybean cotyledons.

    abstract：:Methiocarb, an aromatic-alkyl sulfide insecticide was enzymatically oxidized into its sulfoxide by microsomes from soybean cotyledons. No further oxidation into sulfone was detected. Distribution of the sulfoxidase activity was studied in soybean seedlings and found maximal in cotyledons. Subcellular fractionation of ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(86)91016-8

    authors： Blee E,Durst F

    更新日期：1986-03-28 00:00:00

  • Neonatal pancreatic cells redifferentiate into both neural and pancreatic lineages.

    abstract：:Studies of islet neogenesis have suggested that the regeneration of islet cells can be achieved through redifferentiation of pre-existing islet cells. However, this hypothesis is largely unproven and fails to account for the diversity of observed islet neogenesis. Here we show that cultured neonatal pancreatic cells d...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.10.179

    authors： Zhao W,Hirose T,Ishikawa M,Oshima Y,Hirai S,Ohno S,Taniguchi H

    更新日期：2007-01-05 00:00:00

  • Accumulation of deletions in MtDNA during tissue aging: analysis by long PCR.

    abstract：:Multiple deletions of mtDNA have not only been implicated in aging, but also in a wide variety of pathological conditions. The enzyme system used in long-PCR makes it possible to synthesize the entire mitochondrial genome (16.5 kb), exposing the multiple deletions in mtDNAs implicated in and, at least partially, respo...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.2745

    authors： Reynier P,Malthiery Y

    更新日期：1995-12-05 00:00:00

  • Inhibition of interferon-gamma-activated nuclear factor-kappa B by cyclosporin A: A possible mechanism for synergistic induction of apoptosis by interferon-gamma and cyclosporin A in gastric carcinoma cells.

    abstract：:We previously reported synergistic induction of apoptosis by IFN-gamma plus either cyclosporin A (CsA) or tacrolimus (FK506) in gastric carcinoma cells. In this study, we aimed to elucidate the mechanism for this synergistic induction of apoptosis. IFN-gamma plus CsA synergistically induced caspase-3 mediated apoptosi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(03)00853-2

    authors： Beppu K,Morisaki T,Matsunaga H,Uchiyama A,Ihara E,Hirano K,Kanaide H,Tanaka M,Katano M

    更新日期：2003-06-13 00:00:00

  • Developmentally regulated expression of a nonmuscle myosin heavy chain IIB inserted isoform in rat brain.

    abstract：:The alternatively spliced isoform of the nonmuscle myosin II heavy chain B (MHC-B) with an insert of 21 amino acids at the 50- to 20-kDa junction of the globular region of myosin has been demonstrated to be expressed specifically in the central nervous system (CNS) in chicken. To explore the role of this B2 inserted i...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.0717

    authors： Takahashi M,Hirano T,Uchida K,Yamagishi A

    更新日期：1999-05-27 00:00:00

  • Microarray and gene co-expression analysis reveals that melatonin attenuates immune responses and modulates actin rearrangement in macrophages.

    abstract：:Melatonin produced by the pineal gland suppresses inflammatory responses in innate immune cells. However, the mechanism of how melatonin affects inflammatory gene regulation remains unclear. Here we performed comprehensive microarray analysis combined with transcription factor binding site (TFBS) analysis using LPS-in...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.02.063

    authors： Kadena M,Kumagai Y,Vandenbon A,Matsushima H,Fukamachi H,Maruta N,Kataoka H,Arimoto T,Morisaki H,Funatsu T,Kuwata H

    更新日期：2017-04-01 00:00:00

  • Effects of recombinant human osteogenic protein-1 on the differentiation of osteoclast-like cells and bone resorption.

    abstract：:Recombinant human OP-1 stimulated the formation of tartrate-resistant acid phosphatase (TRAP)-positive multinucleated cells (MNCs) significantly and in a dose-dependent manner in rat bone marrow cell culture. Newly formed MNCs either induced by hOP-1 alone or with 1,25(OH)2D3 were also positive for vitronectin recepto...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.1521

    authors： Hentunen TA,Lakkakorpi PT,Tuukkanen J,Lehenkari PP,Sampath TK,Väänänen HK

    更新日期：1995-04-17 00:00:00

  • Extraction of phosphorylated sperm specific histone H1 from sea urchin eggs: analysis of phosphopeptide maps.

    abstract：:Sea urchin spermatozoa contain a unique histone H1 (SpH1) with an unusual primary structure. Within 12 minutes postinsemination SpH1 is phosphorylated and lost from the chromatin. Both sperm and egg kinases phosphorylate SpH1 in vivo. Until now the analysis of the phosphorylation of SpH1 in eggs in vivo during the fir...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(88)80493-5

    authors： Porter DC,Vacquier VD

    更新日期：1988-03-30 00:00:00

  • Purification and characterization of two haloperoxidases from the glycopeptide antibiotic producer Streptomyces toyocaensis NRRL 15009.

    abstract：:Streptomyces toyocaensis NRRL 15009 produces A47934, a glycopeptide antibiotic. This compound is composed of several unusual amino acids, some of which have chlorinated aromatic rings. We have isolated two distinct halogenating enzymes, a chloroperoxidase (42,882 Da) and a catalase/bromoperoxidase (53,890 Da), from la...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.0276

    authors： Marshall GC,Wright GD

    更新日期：1996-02-15 00:00:00

  • Tissue-specific roles of GCN2 in aging and autosomal dominant retinitis pigmentosa.

    abstract：:The organisms have the capacity to sense and adapt to their surroundings for their life in a dynamic environment. In response to amino acid starvation, cells activate a rectifying physiological program, termed the integrated stress response (ISR), to restore cellular homeostasis. General controlled non-repressed (GCN2...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.09.120

    authors： Kim K,Park JE,Yeom J,Park N,Trần TT,Kang MJ

    更新日期：2020-12-17 00:00:00