Abstract:
AIMS/HYPOTHESIS:The conversion of D-[3-(3)H]glucose to (3) HOH is currently measured to assess D-glucose utilisation. The validity of such a procedure was re-evaluated. METHODS:The conversion of D-[3-(3)H]glucose and D-[5-(3)H]glucose to (3) HOH was measured in rat pancreatic islets, parotid cells and erythrocytes. The tritiation of lipids were also examined in islets exposed to D-[3-(3)H]glucose or D-[5-(3)H]glucose. RESULTS:In rat pancreatic islets and parotid cells, but not in rat erythrocytes, the generation of (3) HOH from D-[3-(3)H]glucose underestimates the rate of D-glucose utilisation, this being apparently attributable to a partial escape from detritiation of [1-(3)H]glycerone-3-phosphate. Such an escape phenomenon resulted in a higher tritiation of lipids in pancreatic islets exposed to D-[3-(3)H]glucose, rather than D-[5-(3)H]glucose. Its relative extent was affected by a number of environmental factors such as the cell type under consideration, the metabolic status of the animals, and the extracellular concentration of D-glucose. CONCLUSION/INTERPRETATION:These findings impose a reservation on the use of D-[3-(3)H]glucose conversion to (3)HOH as a tool to assess the utilisation of the hexose in some cell types.
journal_name
Diabetologiajournal_title
Diabetologiaauthors
Sener A,Giroix MH,Malaisse WJdoi
10.1007/s00125-002-0907-5subject
Has Abstractpub_date
2002-09-01 00:00:00pages
1274-80issue
9eissn
0012-186Xissn
1432-0428journal_volume
45pub_type
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