Abstract:
:The phenotypic and genotypic profiles of the V. cholerae strains causing the Mozambican 1997-8 epidemic were characterized to provide a reference for comparison with other epidemic strains. A total of 75 strains of V. cholerae O1 isolated in different provinces, were analysed. Strains were characterized by PCR for detecting toxin genes (ctxA, zot and ace), virulence associated genes (tcpA. nanH, hlyA and torR) and ERIC sequences. All V. cholerae strains were serotype O1, Ogawa, biotype El Tor. MIC testing showed a high proportion of strains multi-resistant to drugs (100% to cotrimoxazole and 52% to tetracycline) and susceptibility to ciprofloxacin. The isolates contained two intact copies of the CTX genetic element and all other genes tested. PCR of restricted DNA revealed two ERIC types: the first in provincial isolates, also predominant in other African epidemic strains, and the second in Maputo isolates (the national capital).
journal_name
Epidemiol Infectjournal_title
Epidemiology and infectionauthors
Folgosa E,Mastrandrea S,Cappuccinelli P,Uzzau S,Rappelli P,Brian MJ,Colombo MMdoi
10.1017/s0950268801005623subject
Has Abstractpub_date
2001-08-01 00:00:00pages
17-25issue
1eissn
0950-2688issn
1469-4409journal_volume
127pub_type
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