Abstract:
:Phosphorylation and dephosphorylation are key events in protein expression and regulation and in signal transduction. Phosphopeptides are very useful reagents for the study of these processes, and have been used to great advantage in the study of phosphatase substrate specificity, SH2 domain ligand specificity, and protein-protein interactions. Furthermore, the advent of cell-permeable peptide carriers, such as those from the antennapedia homeodomain and the HIV TAT transcription factor, has allowed the study of intracellular events, thus underscoring the utility of these reagents. In this paper we review methods for the synthesis of phosphopeptides with the emphasis on the preparation of phosphoamino acid building blocks.
journal_name
Biopolymersjournal_title
Biopolymersauthors
McMurray JS,Coleman DR 4th,Wang W,Campbell MLdoi
10.1002/1097-0282(2001)60:1<3::AID-BIP1001>3.0.CO;subject
Has Abstractpub_date
2001-01-01 00:00:00pages
3-31issue
1eissn
0006-3525issn
1097-0282pii
10.1002/1097-0282(2001)60:1<3::AID-BIP1001>3.0.CO;journal_volume
60pub_type
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