Abstract:
:A simple solid-state NMR method was used to study the structure of (13)C- and (15)N-enriched silk from two Australian orb-web spider species, Nephila edulis and Argiope keyserlingi. Carbon-13 and (15)N spectra from alanine- or glycine-labeled oriented dragline silks were acquired with the fiber axis aligned parallel or perpendicular to the magnetic field. The fraction of oriented component was determined from each amino acid, alanine and glycine, using each nucleus independently, and attributed to the ordered crystalline domains in the silk. The relative fraction of ordered alanine was found to be higher than the fraction of ordered glycine, akin to the observation of alanine-rich domains in silk-worm (Bombyx mori) silk. A higher degree of crystallinity was observed in the dragline silk of N. edulis compared with A. keyserlingi, which correlates with the superior mechanical properties of the former.
journal_name
Biopolymersjournal_title
Biopolymersauthors
Bonev B,Grieve S,Herberstein ME,Kishore AI,Watts A,Separovic Fdoi
10.1002/bip.20471subject
Has Abstractpub_date
2006-06-05 00:00:00pages
134-43issue
2eissn
0006-3525issn
1097-0282journal_volume
82pub_type
杂志文章相关文献
BIOPOLYMERS文献大全abstract::Nuclear magnetic resonance and optical experiments are combined to determine the rms amplitude of local angular motion of purines in DNA in solution. A 12 base-pair duplex DNA with the sequence d(CGCGAATTCGCG)2 is deuterated at the H8 positions of adenine and guanine by exchange with solvent at 55 degrees C. The deute...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360340404
更新日期:1994-04-01 00:00:00
abstract::The absorption, CD, and fluorescence emission spectra, and the fluorescence emission and depolarization lifetimes of the human granulocyte-macrophage colony-stimulating factor (hGM-CSF) and related peptides previously tested for their immunological activity, were measured in water at various pHs and temperatures to ob...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360360102
更新日期:1995-07-01 00:00:00
abstract::The conformation of thymosin beta 9 in solution of 40% (v/v) 1,1,1,3,3,3-hexafluoro-2-propanol-d2 in water has been investigated by two-dimensional 1H-nmr spectroscopy. Under this condition thymosin beta 9 adopts an ordered structure. The determination of the conformation of the peptide was based on a set of 304 appro...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0282(199705)41:6<623::AID-BIP3>
更新日期:1997-05-01 00:00:00
abstract::The interaction of the immunogenic peptide of human hepatitis B virus (HBV) preS(120-145), including B and T epitopes, with phospholipid vesicles has been studied by fluorescence techniques and CD. In addition, interaction of three lipopeptides derived from preS(120-145) containing stearoyl, cholanoyl, and tripalmitoy...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(sici)1097-0282(199605)38:5<607::aid-bip6>
更新日期:1996-05-01 00:00:00
abstract::To investigate the mechanism leading to the reduction in tensile strength of permanent waved human hair, the structure of cross-sections at various depths of permanent waved white human hair was directly analyzed without isolating the cuticle and cortex, using Raman spectroscopy. The beta-sheet and/or random coil cont...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20646
更新日期:2007-02-15 00:00:00
abstract::Molecular modeling is used to demonstrate that the major structural deformations of DNA caused by four different minor groove binding proteins, TBP, SRY, LEF-1, and PurR, can all be mimicked by stretching the double helix between two 3'-phosphate groups flanking the binding region. This deformation reproduces the wide...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0282(19990415)49:5<341::AID-BIP
更新日期:1999-04-15 00:00:00
abstract::The presence of low pKa N-hydroxylamines is beneficial in peptide chemistry as they reduce some base-mediated side reactions. Here we evaluated the applicability and buffering capacity of Ethyl 2-cyano-2-(hydroxyimino) acetate (Oxyma) in the prevention of aspartimide/piperidide formation and Pro-based overcoupling and...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21713
更新日期:2012-01-01 00:00:00
abstract::Since the advent of single particle/molecule microscopies, researchers have applied these techniques to understanding the fluid membranes of cells. By observing diffusion of membrane proteins and lipids in live cell membranes of eukaryotic cells, it has been found that membranes contain a mosaic of fluid compartments....
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.20801
更新日期:2007-10-05 00:00:00
abstract::The advent of kinetically controlled Native Chemical Ligation has permitted more efficient sequential Native Chemical Ligation reactions to take place in one pot where one or more of the peptide fragments contains an N-terminal cysteine residue and a C-terminal thioester. The reactivity of the thioester component can ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21473
更新日期:2010-01-01 00:00:00
abstract::Lung cancer is usually fatal once it becomes metastatic. However, in order to develop metastases, a tumor usually invades the basal membrane and enters the vascular or lymphatic system. In this study, a three-dimensional artificial membrane using collagen type I, one of the main components of basal membranes, was esta...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20297
更新日期:2005-08-15 00:00:00
abstract::A large number of potent and selective therapeutic agents, useful for the treatment of several diseases, have been isolated from natural sources. For example, the most active thrombin inhibitors are those secreted by the salivary glands of leeches. One peculiar feature of these agents is the lack of any significant in...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0282(1999)51:1<19::AID-BIP4>3.0
更新日期:1999-01-01 00:00:00
abstract::A new representation of protein sequence is devoted in this paper, in which each protein can be represented by a 20-dimensional (20D) vector of unit length. Inspired by the principle of superposition of state in quantum mechanics, the squares of the 20 components of the vector correspond to the amino acid composition....
journal_title:Biopolymers
pub_type:
doi:10.1002/1097-0282(20010415)58:5<491::AID-BIP1024>3
更新日期:2001-04-15 00:00:00
abstract::We report on an unrestrained molecular dynamics simulation of the flavin mononucleotide (FMN)-RNA aptamer. The simulated average structure maintains both cross-strand and intermolecular FMN-RNA nuclear Overhauser effects from the nmr experiments and has all qualitative features of the nmr structure including the G10-U...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0282(199909)50:3<287::AID-BIP5>
更新日期:1999-09-01 00:00:00
abstract::Fluorescein-labeled antibodies are widely used in clinical assays and fluorescence microscopy. The fluorescent signal per labeled antibody is limited by fluorescein self-quenching, which occurs when the antibody is heavily labeled with multiple fluoresceins. We examined immunoglobulin G (IgG) when labeled with 0.7 to ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20098
更新日期:2004-08-15 00:00:00
abstract::Disintegrins, small molecular weight proteins contained in the venom of vipers and rattlesnakes, are high-affinity and selectivity integrin antagonists. Disintegrins inhibitory epitope mainly consists in a tripeptide sequence localized in a mobile loop protruding from the protein core. RTS and/or KTS tripeptide charac...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22578
更新日期:2015-03-01 00:00:00
abstract::Relevant parameters and stereochemical consequences of helices [alpha-helix, 3(10)-helix, beta-bend ribbon spiral, gamma-helix, 2.0(5)-helix, poly(Pro)(n) type-I and -II helices, and collagen triple helix] of peptides based on alpha-amino acids for use as templates in various branches of chemistry are briefly discusse...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.20357
更新日期:2006-01-01 00:00:00
abstract::We have utilized a de novo designed two-stranded α-helical coiled-coil template to display conserved α-helical epitopes from the stem region of hemagglutinin (HA) glycoproteins of influenza A. The immunogens have all the surface-exposed residues of the native α-helix in the native HA protein of interest displayed on t...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22808
更新日期:2016-03-01 00:00:00
abstract::While end capping in alpha-helices is well understood, the concept of capping a beta-hairpin is a relatively recent development; to date, favorable Coulombic interactions are the only example of sidechains at the termini influencing the overall stability of a beta-hairpin. While cross-strand hydrophobic residues gener...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21177
更新日期:2009-07-01 00:00:00
abstract::The linear viscoelastic and rheological properties of high molecular weight ovine submaxillary mucin (OSM) solution have been investigated in terms of the Newtonian steady-flow viscosity [eta(gamma)], the complex oscillatory viscosity [eta*(omega)], and the storage and loss shear moduli [G'(omega) and G"(omega)]. It w...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360291004
更新日期:1990-08-15 00:00:00
abstract::Spray drying is a way to generate protein solids (powders), which is also true for lyophilization. Sugars are used to protect proteins from conformational changes and chemical degradations arising from drying processes and storage conditions such as the humidity. The influence of trehalose and humidity on the conforma...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10558
更新日期:2004-03-01 00:00:00
abstract::Additive physical properties of DNA double strand polymers have been expanded in terms of 8 irreducible parameters. This provided consistency relations among the corresponding 10 duplex dimer contributions. To allow for oligomer analysis, end parameters were often added, and this would add extra degrees of freedom to ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22214
更新日期:2013-08-01 00:00:00
abstract::The design, synthesis, and evaluation of a novel series of receptors for protein surface recognition are described. The design of these agents is based around the attachment of four constrained peptide loops onto a central calix[4]arene scaffold. This arrangement mimics the role of the hypervariable loops in antibody ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0282(1998)47:4<285::AID-BIP4>3.
更新日期:1998-01-01 00:00:00
abstract::Interactions between the diketopiperazine cyclo-alanylglycine and four fluorinated alcohols in water-fluoroalcohol mixtures were examined by (1)H[(19)F] intermolecular nuclear Overhauser effects (NOE) experiments. The alcohols studied were trifluoroethanol, hexafluoroacetone trihydrate, 1,1,1,3,3,3-hexafluoroisopropan...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10176
更新日期:2002-08-15 00:00:00
abstract::Simulated ir absorption and vibrational CD (VCD) spectra of four alanine-based octapeptides, each having its main chain constrained to a different secondary structure conformation, were analyzed and compared with experimental results for several different peptides. The octapeptide simulations were based on transfer of...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0282(20000415)53:5<380::AID-BIP
更新日期:2000-04-15 00:00:00
abstract::We describe a method to determine the magnitude of protein-induced DNA bends relative to a set of standard A tract bends using comparative gel electrophoresis. The DNA bend of interest was that induced by the catabolite activator protein (CAP), the transcriptional activator protein of the lac operon. The set of compar...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360290106
更新日期:1990-01-01 00:00:00
abstract::The formation of the KRas4B-PDEδ complex activates different signaling pathways required for the development and maintenance of cancer. Previous experimental and theoretical studies have allowed researchers to design an inhibitor of the KRas4B-PDEδ complex, "Deltarasin." This inhibitor binds to the prenyl-binding pock...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.23333
更新日期:2019-11-01 00:00:00
abstract::Hemochromatosis factor E (HFE) is a member of class I MHC family and plays a significant role in the iron homeostasis. Denaturation of HFE induced by guanidinium chloride (GdmCl) was measured by monitoring changes in [θ]222 (mean residue ellipticity at 222 nm), intrinsic fluorescence emission intensity at 346 nm (F346...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22760
更新日期:2016-03-01 00:00:00
abstract::A natural rubber was identified and characterized for the first time in the latex of the perennial Mediterranean shrub Euphorbia characias. Four different methods, i.e., acetone, acetic acid, trichloroacetic acid, and Triton® X-100, followed by successive treatments with cyclohexane/ethanol, were employed to extract t...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22044
更新日期:2012-08-01 00:00:00
abstract::We prepared the water soluble model peptide, (E)(8) GGLGGQGAG(A)(6) GGAGQGGYGG, to throw light on the local structure of spidroin 1 (MaSpl) protein in spider dragline silk of Nephila clavipes before and after spinning. Solution (13) C NMR showed that the conformation of the peptide in aqueous solution was essentially ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21718
更新日期:2012-06-01 00:00:00
abstract::The kinetics of the hydrodynamic volume change associated with the unfolding and refolding of a globular protein can be observed using high performance size exclusion chromatography. Chromatographic profiles that evidence such dynamics can be simulated using equations in which chromatographic partitioning and the conf...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360330113
更新日期:1993-01-01 00:00:00