Abstract:
:A natural rubber was identified and characterized for the first time in the latex of the perennial Mediterranean shrub Euphorbia characias. Four different methods, i.e., acetone, acetic acid, trichloroacetic acid, and Triton® X-100, followed by successive treatments with cyclohexane/ethanol, were employed to extract the natural rubber. The rubber content was shown to be 14% (w/v) of the E. characias latex, a low content compared with that of Hevea brasiliensis (30-35%) but a similar content to other rubber producing plants. E. characias rubber showed a molecular weight of 93,000 with a M(w) /M(n) of 2.9. (1) H NMR, (13) C NMR, and FTIR analysis revealed the characteristic of the cis-1,4-polyisoprene typical of natural rubber. These results provided novel insight into latex components and will ultimately benefit the broader understanding of E. characias latex composition.
journal_name
Biopolymersjournal_title
Biopolymersauthors
Spanò D,Pintus F,Mascia C,Scorciapino MA,Casu M,Floris G,Medda Rdoi
10.1002/bip.22044subject
Has Abstractpub_date
2012-08-01 00:00:00pages
589-94issue
8eissn
0006-3525issn
1097-0282journal_volume
97pub_type
杂志文章相关文献
BIOPOLYMERS文献大全abstract::An increasing number of RNAs are being discovered that perform their functions by undergoing large changes in conformation in response to a variety of cellular signals, including recognition of proteins and small molecular targets, changes in temperature, and RNA synthesis itself. The measurement of NMR residual dipol...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.20765
更新日期:2007-08-05 00:00:00
abstract::The transient electric birefringence of two small DNA restriction fragments of the same molecular weight, one of which migrates anomalously slowly on polyacrylamide gels, has been investigated. Both fragments exhibit negative birefringence. The decay of the birefringence of the anomalously slowly migrating fragment is...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360311318
更新日期:1991-11-01 00:00:00
abstract::Since the advent of single particle/molecule microscopies, researchers have applied these techniques to understanding the fluid membranes of cells. By observing diffusion of membrane proteins and lipids in live cell membranes of eukaryotic cells, it has been found that membranes contain a mosaic of fluid compartments....
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.20801
更新日期:2007-10-05 00:00:00
abstract::The solution conformations of a hybrid sequence peptide related to the bee venom peptide apamin have been determined using two-dimensional 1H-nmr. Apamin is an 18 amino acid peptide containing a C-terminal helix that is stabilized by two disulfide bonds. The deletion of one residue (K4) of the N-terminal "scaffold" re...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0282(19970405)41:4<451::AID-BIP
更新日期:1997-04-05 00:00:00
abstract::Natural cellulose molecules have a strong tendency of being aggregated into larger structures. Thus, the imaging of isolated individual cellulose molecules is hampered for a long time. In this work, we manage to observe, for the first time, the isolated individual natural cellulose chains on a sample surface by means ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21070
更新日期:2008-12-01 00:00:00
abstract::The coupling of nuC-O and deltaO-D vibrations in the 1200-900 cm(-1) IR range leads to band shifting in opposite directions, which provides information on intramolecular hydrogen bonding of carbohydrates in aqueous solution. The aqueous solution IR spectra of 2-acetamide-1,6-anhydro-2-deoxy-D-glucopyranose and cis-1,2...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10024
更新日期:2002-01-01 00:00:00
abstract::Nocistatin, a new heptadecapeptide encoded in the bPNP-3 gene, has a powerful biological activity connected with the mechanisms of pain transmission. It does not bind to the opioid receptors but to another brain receptor with high affinity. In order to substantiate these novel biological data with a structural basis, ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0282(200003)53:3<257::AID-BIP5>
更新日期:2000-03-01 00:00:00
abstract::Ultraviolet hyperchromicity experiments indicate that in DNA duplex formation, a C-T mismatch is destabilizing in the center of a duplex, but behaves as a stable base pair at the terminus of a duplex. The C-T base pair is thought to contain two hydrogen bonds, but has thermodynamic parameters (delta Ho and delta Go of...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360310908
更新日期:1991-08-01 00:00:00
abstract::The ion atmosphere created by monovalent (Na(+) ) or divalent (Mg(2+) ) cations surrounding a B-form DNA duplex were examined using atomistic molecular dynamics (MD) simulations and the nonlinear Poisson-Boltzmann (PB) equation. The ion distributions predicted by the two methods were compared using plots of radial and...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22461
更新日期:2014-08-01 00:00:00
abstract::Aromatic stacking and hydrogen bonding between nucleobases are two of the key interactions responsible for stabilization of DNA double-helical structures. The present work aims at defining the specific contributions of these interactions to the stability of individual base pairs in DNA. The two DNA double helices inve...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20811
更新日期:2007-10-05 00:00:00
abstract::The physicochemical characteristics of the green fluorescent protein (GFP), including the thermodynamic properties (entropy, enthalpy, Gibbs' free energy, heat capacity), normal mode vibrations, and atomic fluctuations, were investigated. The Gaussian 03 computational chemistry program was employed for normal mode ana...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20264
更新日期:2005-06-15 00:00:00
abstract::In this paper, we studied the quenching mechanism of intrinsic fluorescence of type I collagen by a new type photosensitizer and fluorescence quencher, hypocrellin B (HB). It was indicated that type I collagen can emit Tyr-intrinsic fluorescence with the excitation wavelength of Tyr (lambda(ex) = 269 nm). Its fluoresc...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0282(199708)42:2<219::AID-BIP10
更新日期:1997-08-01 00:00:00
abstract::Hybrids of the double-chain bis-cystinyl fragment 225-232/225'-232' of human immunoglobulin G1 (IgG1) with the human little-gastrin sequence 2-17 were found to induce in animals a strong antigastrin humoral immune response with antibody titers comparable to those raised with conventional gastrin/carrier-protein conjug...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360310603
更新日期:1991-05-01 00:00:00
abstract::The crystal and molecular structure of the pentapeptide Boc-D-Ala-delta Phe-Gly-delta Phe-D-Ala-OMe, containing two dehydrophenylalanine residues, was determined by x-ray diffraction. The molecule crystallizes in the orthorombic P2(1)2(1)2(1) space group, with a = 10.439(3), b = 15.319(3) and c = 21.099(4) A. In the s...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360300906
更新日期:1990-01-01 00:00:00
abstract::The role of phosphorylation in stabilizing the N-termini of alpha-helices is examined using computer simulations of model peptides. The models comprise either a phosphorylated or unphosphorylated serine at the helix N-terminus, followed by nine alanines. Monte Carlo/stochastic Dynamics simulations were performed on th...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0282(199903)49:3<225::AID-BIP4>
更新日期:1999-03-01 00:00:00
abstract::The experimental thermodynamic data for the dissolution of five simple hydrocarbon molecules in water were combined with the solute-solvent interaction energy from a computer simulation study to yield data on the enthalpy change of solvent reorganization. Similar data were generated for dissolving these same solute mo...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360310809
更新日期:1991-07-01 00:00:00
abstract::In order to check our current knowledge on the principles involved in beta-hairpin formation, we have modified the sequence of a 3:5 beta-hairpin forming peptide with two different purposes, first to increase the stability of the formed 3:5 beta-hairpin, and second to convert the 3:5 beta-hairpin into a 2:2 beta-hairp...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20345
更新日期:2005-10-15 00:00:00
abstract::This report details the observation of (a) the intact double helix of DNA at 0% relative humidity in poly(dA).poly(dT) and poly(dA-dT).poly(dA-dT) films, (b) the fractal-like growth of DNA crystals in films of poly(dA).poly(dT), and (c) poly(dA).poly(dT) with adenines in the B form and thymines in the A form. Observat...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360340814
更新日期:1994-08-01 00:00:00
abstract::Apelin is the endogenous ligand of APJ, which belongs to the superfamily of G protein-coupled receptors. In recent years, the apelin/APJ system has been detected in many tissues and emerges as a promising target for the treatment of various pathophysiological conditions. Pyr1-apelin-13 is the major isoform of apelin i...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22498
更新日期:2014-07-01 00:00:00
abstract::Side-chain lactam bridges linking amino acid residues that are spaced several residues apart in the linear sequence offer a convenient and flexible method for introducing conformational constraints into a peptide structure. The availability of a variety of selectively cleavable protecting groups for amines and carboxy...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.10203
更新日期:2002-01-01 00:00:00
abstract::Four members of the carcinoembryonic antigen family, CEACAMs 1, 3, 6, and 8, are expressed on human neutrophils and can trigger an activation signal that increases neutrophil adhesion to human umbilical vein endothelial cell (HUVEC) monolayers. To identify active sites on CEACAM1, we previously performed molecular mod...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21447
更新日期:2011-01-01 00:00:00
abstract::Individual ethidium-stained DNA molecules, embedded in an agarose gel made with electrophoresis buffer (0.05 molar salt), are observed using a fluorescence microscope. In the first experiment, open circular 66 kilobase pair (kbp) plasmids, immobilized by agarose fibers threaded through their centers, display entropic ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360290807
更新日期:1990-07-01 00:00:00
abstract::Elucidation of mechanisms underlying collagen fibril assembly and matrix-induced guidance of cell fate will contribute to the design and expanded use of this biopolymer for research and clinical applications. Here, we define how Type I collagen oligomers affect in-vitro polymerization kinetics as well as fibril micros...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21537
更新日期:2011-02-01 00:00:00
abstract::Tissue engineering techniques particularly using electrospun scaffolds have been intensively used in recent years for the development of small diameter vascular grafts. However, the development of a completely successful scaffold that fulfills multiple requirements to guarantee complete vascular regeneration remains c...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22524
更新日期:2014-12-01 00:00:00
abstract::A simple solid-state NMR method was used to study the structure of (13)C- and (15)N-enriched silk from two Australian orb-web spider species, Nephila edulis and Argiope keyserlingi. Carbon-13 and (15)N spectra from alanine- or glycine-labeled oriented dragline silks were acquired with the fiber axis aligned parallel o...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20471
更新日期:2006-06-05 00:00:00
abstract::Combinatorial library composed of rigid rod-like peptides with a triple-helical scaffold was constructed. The component peptides were designed to have various combinations of basic and neutral (or hydrophobic) amino acid residues based on collagen-like (Gly-Pro-Yaa)-repeating sequences, inspired from the basic and amp...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22791
更新日期:2016-11-04 00:00:00
abstract::The visual pigment rhodopsin has been extensively studied for the kinetics of its photointermediates by various spectroscopic methods. Unlike such archaeal retinal proteins as bacteriorhodopsin, visual rhodopsin does not thermally recover its dark state after photoexcitation, which precludes repeated excitation of a s...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20540
更新日期:2006-10-05 00:00:00
abstract::Short peptides composed of phenylalanine and sequences derived from amyloidogenic peptides have the ability to self-assemble to form nanostructures including hydrogels. The self-assembly of peptides composed of only hydrophobic amino acids and aliphatic protecting groups have not been investigated in detail. We have e...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.23033
更新日期:2017-11-01 00:00:00
abstract::The general properties of hydroxyethylcellulose (HEC) grafted with 3-glycidoxypropyltrimethoxysilane (GPTMS) or 3-glycidoxypropylmethyldiethoxysilane (GPDMS) were studied for potential biomedical applications. The graft involved a Williamson reaction between the free hydroxyl function of HEC and the epoxy function of ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10053
更新日期:2002-04-05 00:00:00
abstract::The development of differential scanning calorimetry has resulted in an increased interest in studies of the unfolding process in proteins with the aim of identifying domains and interactions with ligands or other proteins. Several of these studies were done with actin and showed that the thermal unfolding of F-actin ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20567
更新日期:2006-11-01 00:00:00