Abstract:
:The solution conformations of a hybrid sequence peptide related to the bee venom peptide apamin have been determined using two-dimensional 1H-nmr. Apamin is an 18 amino acid peptide containing a C-terminal helix that is stabilized by two disulfide bonds. The deletion of one residue (K4) of the N-terminal "scaffold" region of the apamin sequence results in a helical peptide, but with a change in the pairing of cysteines to form the disulfide cross links. The new disulfide arrangement is analogous to that of the vasoconstrictor peptide endothelin. Two sets of nmr resonances were observed for the apamin-deletion (AD) peptide, due to cistrans isomerism at the A4-P5 peptide bond. The cis isomer of the AD peptide contains a tight turn in residues 3-6, which is required for formation of the alpha-helix in residues 7-15. Nuclear Overhauser effects observed for the trans AD peptide are not consistent with any single unique fold, indicating the presence of conformational averaging when the peptide adopts the trans form. Distance geometry calculations on the cis AD peptide reveal an alpha-helical structure that appears to be more like that of apamin than the crystal structure of human endothelin, despite the reversal of the disulfide pattern in the AD peptide from that of apamin to that of endothelin.
journal_name
Biopolymersjournal_title
Biopolymersauthors
Volkman BF,Wemmer DEdoi
10.1002/(SICI)1097-0282(19970405)41:4<451::AID-BIPsubject
Has Abstractpub_date
1997-04-05 00:00:00pages
451-60issue
4eissn
0006-3525issn
1097-0282pii
10.1002/(SICI)1097-0282(19970405)41:4<451::AID-BIPjournal_volume
41pub_type
杂志文章相关文献
BIOPOLYMERS文献大全abstract::G protein-coupled receptor kinase 2 (GRK2) plays a central role in the cellular transduction network. In particular, during chronic heart failure GRK2 is upregulated and believed to contribute to disease progression. Thereby, its inhibition offers a potential therapeutic solution to several pathological conditions. In...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22295
更新日期:2014-01-01 00:00:00
abstract::The (Fe2S2)2+ complex of an artificial 20-peptide ligand, Ac-Pro-Tyr-Ser-Cys-Arg-Ala-Gly-Ala-Cys-Ser-Thr-Cys-Ala-Gly-Pro-Leu-Leu-T hr-Cys- Val-NH2, containing an invariant Cys-A-B-C-D-Cys-X-Y-Cys (A, B, C, D, X, Y = amino acid residues) fragment of plant-type ferredoxins was synthesized by a ligand exchange method wit...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360321111
更新日期:1992-11-01 00:00:00
abstract::13C and (31)P NMR spectra of a transmembrane peptide, [1-(13)C]Ala(14)-labeled A(6-34), of bacteriorhodopsin incorporated into dimyristoylphosphatidylcholine (DMPC) bilayer were recorded to clarify its dynamics and orientation in the lipid bilayer. This peptide is shown to take an alpha-helical form both in liquid cry...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10021
更新日期:2002-02-01 00:00:00
abstract::IR vibrational CD (VCD) has been observed for the cyclic pentapeptide cyclo-(-Gly-Pro-Gly-D-Ala-Pro-) in solution in CDBr3. The observed VCD spectra do not resemble the VCD features of any of the previously reported peptide secondary structures, such as alpha-helical, "random coil," or sheet structures, and might be d...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360320912
更新日期:1992-09-01 00:00:00
abstract::Ubiquitin (Ub) is a small 76 amino acid long protein that is highly conserved in all eukaryotes studied to date. In humans, more than 600 ligases are involved in the reversible modification of specific lysine side-chain amines in substrate proteins by conjugation with the C-terminal carboxylate of Ub. Initially monoub...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.22253
更新日期:2014-02-01 00:00:00
abstract::The conformation of basic fatty acid binding protein from chicken liver and the binding properties of the apo protein toward 11-dansylamino-undecanoic acid were investigated by CD and fluorescence spectroscopy. In one set of experiments the binding process was followed by the appearance of induced optical activity in ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360340707
更新日期:1994-07-01 00:00:00
abstract::Depsipeptide analogues of peptide sequences can help in elucidating the role of specific hydrogen bonds in determining the conformation in peptides. The repeating pentapeptide and hexapeptide sequences of elastin have been suggested to contain a type II beta-turn with a 4----1 hydrogen bond. Depsipeptide analogues of ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360291212
更新日期:1990-10-01 00:00:00
abstract::Insect neuropeptides of the insect kinin class share a common C-terminal pentapeptide sequence F(1)X(1)(2)X(2)(3)W(4)G(5)-NH(2) (X(2)(3) = P, S, A) and regulate such critical physiological processes as water balance and digestive enzyme release. Analogs of the insect kinin class, in which the critical residues of F(1)...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20638
更新日期:2007-01-01 00:00:00
abstract::Alpha-synuclein (alpha-Syn) has been identified as a component of intracellular fibrillar deposits in Parkinson's disease. Though the real pathogenesis is still unknown, many investigations have revealed that conformational alteration and fibril formation of alpha-Syn protein have an important role in causing the dise...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20550
更新日期:2006-10-15 00:00:00
abstract::The hinge region links the antigen binding Fab part to the constant Fc domain in immunoglobulins. For the hinge peptide derivative [AcThr(OtBu)-Cys-Pro-Pro-Cys-Pro-Ala-ProNH2]2 the assignment of the 1H and 13C resonances was achieved by two-dimensional nmr techniques: total correlation spectroscopy (TOCSY), nuclear Ov...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360311007
更新日期:1991-09-01 00:00:00
abstract::TC5b is a 20 residue polypeptide notable for its compact tertiary structure, a rarity for a short peptide. This structure is due to the "Trp-cage" motif, an association of aromatic, Pro, and Gly residues. The structure of TC5b has been fully characterized by NMR and electronic circular dichroism (ECD) studies, but has...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20709
更新日期:2007-01-01 00:00:00
abstract::Polymeric macromolecules, when viewed abstractly as strings of symbols, can be treated in terms of formal language theory, providing a mathematical foundation for characterizing such strings both as collections and in terms of their individual structures. In addition this approach offers a framework for analysis of ma...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.22101
更新日期:2013-03-01 00:00:00
abstract::Optical melting curves of 22 DNA dumbbells with the 16-base pair duplex sequence 5'-G-C-A-T-C-A-T-C-G-A-T-G-A-T-G-C-3' linked on both ends by single-strand loops of A, or C, sequences (iota = 2, 3, 4, 6, 8, 10, 14). T sequences (iota = 2, 3, 4, 6, 8, 10), and G iota sequences (iota = 2, 4) were measured in phosphate b...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0282(199612)39:6%3C779::AID-BIP
更新日期:1996-12-01 00:00:00
abstract::An increasing number of RNAs are being discovered that perform their functions by undergoing large changes in conformation in response to a variety of cellular signals, including recognition of proteins and small molecular targets, changes in temperature, and RNA synthesis itself. The measurement of NMR residual dipol...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.20765
更新日期:2007-08-05 00:00:00
abstract::The presence of low pKa N-hydroxylamines is beneficial in peptide chemistry as they reduce some base-mediated side reactions. Here we evaluated the applicability and buffering capacity of Ethyl 2-cyano-2-(hydroxyimino) acetate (Oxyma) in the prevention of aspartimide/piperidide formation and Pro-based overcoupling and...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21713
更新日期:2012-01-01 00:00:00
abstract::A conformational study of poly-L-serine has shown that it can exist in the left-handed α-helical form. A study of a pair of peptide units with the serine sidegroup attached to the α carbon atom linking the two units showed that OH ⃛O hydrogen bonds between the OH group of the side chain and a carbonyl oxygen of the f...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.1968.360060403
更新日期:1968-04-01 00:00:00
abstract::Calcium ions (Ca(2+) ) play key roles in various fundamental biological processes such as cell signaling and brain function. Molecular dynamics (MD) simulations have been used to study such interactions, however, the accuracy of the Ca(2+) models provided by the standard MD force fields has not been rigorously tested....
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22868
更新日期:2016-10-01 00:00:00
abstract::The interaction of an RGD-containing epitope from the hepatitis A virus VP3 capsid protein and its RGA-analogue with lipid membranes was studied by biophysical methods. Two types of model membrane were used: vesicles and monolayers spread at the air/water interface, with a composition that closely resembles the lipid ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/1097-0282(200101)58:1<63::AID-BIP70>3.0.CO
更新日期:2001-01-01 00:00:00
abstract::The ability of an exopolymer of glycoproteic character (GP) excreted by a new gram-negative species Pseudoalteromonas antarctica NF(3), to coat phosphatidylcholine (PC) liposomes and to protect these bilayers against the action of the nonionic surfactant dodecyl maltoside was investigated. Transmission electron micros...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0282(199911)50:6<579::AID-BIP2>
更新日期:1999-11-01 00:00:00
abstract::Apelin is the endogenous ligand of APJ, which belongs to the superfamily of G protein-coupled receptors. In recent years, the apelin/APJ system has been detected in many tissues and emerges as a promising target for the treatment of various pathophysiological conditions. Pyr1-apelin-13 is the major isoform of apelin i...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22498
更新日期:2014-07-01 00:00:00
abstract::Intermolecular NOE experiments have been used to explore interactions of water and ethanol molecules in 35% ethanol/65% water (v/v) with the peptide Trp-cage at temperatures from 5 to 25 degrees C. Magnetic dipole-dipole cross-relaxation terms sigma(HH) (NOE) and sigma(HH) (ROE) for interaction of solvent components w...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21028
更新日期:2008-10-01 00:00:00
abstract::Relevant parameters and stereochemical consequences of helices [alpha-helix, 3(10)-helix, beta-bend ribbon spiral, gamma-helix, 2.0(5)-helix, poly(Pro)(n) type-I and -II helices, and collagen triple helix] of peptides based on alpha-amino acids for use as templates in various branches of chemistry are briefly discusse...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.20357
更新日期:2006-01-01 00:00:00
abstract::This work theoretically investigates the mechanical properties of a novel silk-derived biopolymer as polymerized in silico from sericin and elastin-like monomers. Molecular Dynamics simulations and Steered Molecular Dynamics were the principal computational methods used, the latter of which applies an external force o...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22494
更新日期:2014-09-01 00:00:00
abstract::Bleomycins A(2) and B(2) are the two active components in the antineoplastic drug Blenoxane. DNA is targeted by this drug in cancer cells and the mode of action of this drug involves DNA binding. Ambiguity exists as to the way in which bleomycin binds to DNA. Raman spectroscopy was used to examine both calf thymus DNA...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/1097-0282(1999)52:3<110::AID-BIP20>3.0.CO;
更新日期:1999-01-01 00:00:00
abstract::The occurrence of a highly conserved 11-mer repeating motif in the primary sequence is a major characteristic of group 3 late embryogenesis abundant (LEA3) proteins, which are strongly associated with abiotic stress tolerance of the plants. In this study, the three-dimensional structure, mimetic membrane association, ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21693
更新日期:2012-01-01 00:00:00
abstract::Circular dichroism using synchrotron radiation (SRCD) can extend the spectral range down to approximately 130 nm for dry proteins, potentially providing new structural information. Using a selection of dried model proteins, including alpha-helical, beta-sheet, and mixed-structure proteins, we observe a low-wavelength ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21011
更新日期:2008-09-01 00:00:00
abstract::The x-ray conformations of 5-, 7-, 9-, and 12-residue loops in bovine pancreatic trypsin inhibitor (BPTI) were predicted by the use of multiple independent Monte Carlo simulating annealing (MCSA) runs starting from random conformations. Four buried water molecules interacted with a 12-residue loop that started at resi...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/1097-0282(20010405)58:4<359::AID-BIP1013>3
更新日期:2001-04-05 00:00:00
abstract::In the past two decades, keratin biomaterials have shown impressive results as scaffolds for tissue engineering, wound healing, and nerve regeneration. In addition to its intrinsic biocompatibility, keratin interacts with specific cell receptors eliciting beneficial biochemical cues. However, during extraction from na...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.23037
更新日期:2017-10-01 00:00:00
abstract::Successful intracellular delivery of various bioactive molecules has been reported using cell-permeating peptides (CPPs) as delivery vectors. To determine the effects of CPPs on the cellular uptake of immunoglobulin Fab fragment, conjugates of a radio-iodinated Fab fragment with CPPs (CPP-(125)I-Fab) derived from HIV-...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20689
更新日期:2007-01-01 00:00:00
abstract::The indirect solvent-induced effect on the free energy of binding of biopolymers is examined within the framework of classical statistical mechanics. We focus specifically on the role of the solute-solvent hydrogen bonding. In particular, we have estimated the first order solvent effect on the indirect interaction bet...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360290604
更新日期:1990-05-01 00:00:00