A systematic approach toward the analysis of drug-DNA interactions using Raman spectroscopy: the binding of metal-free bleomycins A(2) and B(2) to calf thymus DNA.

abstract：:The HPLC retention behavior of three complete single methionine and methionine sulfoxide replacement sets of two 18-mer model peptides and neuropeptide Y (NPY) were investigated. All peptides were prepared by multiple solid-phase peptide synthesis. Plotting the retention time differences between methionine and methion...

journal_title：Biopolymers

authors： Rothemund S,Krause E,Beyermann M,Bienert M,Sykes BD,Sönnichsen FD

更新日期：1996-08-01 00:00:00

abstract：:Infrared spectra of poly(D,L-alanine), poly(L-glutamic acid), poly(L-lysine), silk fibroin, and tropomyosin have been registered for various conformations of the polypeptide chain. Assuming additivity of the main- and side-chain absorption, spectral parameters of amide I and II absorption bands corresponding to alpha-...

journal_title：Biopolymers

authors： Venyaminov SYu,Kalnin NN

更新日期：1990-01-01 00:00:00

abstract：:In recent years, a revolution has occurred in the basic sciences, which exploits novel single molecule detection and manipulation tools to track and analyze biopolymers in unprecedented detail. A recent Gordon Research Conference style meeting, hosted by the University of Michigan, highlighted current status and futur...

journal_title：Biopolymers

authors： Walter NG,Meiners JC,Meyhofer E,Neubig RR,Sunahara RK,Perkins NC,Steel DG,Swanson JA

更新日期：2007-02-05 00:00:00

abstract：:Solid state nmr methods were applied to the study of the motions and structural heterogeneity in polyglycine, sodium poly(L-glutamate), and poly(L-alanine). The response of both the main-chain and side-chain resonances to the addition of water was studied using static and magic-angle sample-spinning line shapes as wel...

journal_title：Biopolymers

authors： Kennedy SD,Bryant RG

更新日期：1990-01-01 00:00:00

abstract：:Relevant parameters and stereochemical consequences of helices [alpha-helix, 3(10)-helix, beta-bend ribbon spiral, gamma-helix, 2.0(5)-helix, poly(Pro)(n) type-I and -II helices, and collagen triple helix] of peptides based on alpha-amino acids for use as templates in various branches of chemistry are briefly discusse...

journal_title：Biopolymers

authors： Crisma M,Formaggio F,Moretto A,Toniolo C

更新日期：2006-01-01 00:00:00

abstract：:Studies of catalytically active DNA sequences have expanded considerably since the first artificial deoxyribozyme was identified in 1994. Nevertheless, the field is still quite young, and advances in both fundamental understanding and practical applications of deoxyribozymes are still developing. Deoxyribozymes that e...

journal_title：Biopolymers

authors： Höbartner C,Silverman SK

更新日期：2007-12-05 00:00:00

abstract：:In this article, we review current knowledge about the silk produced by the larvae of bees, ants, and hornets [Apoidea and Vespoidea: Hymenoptera]. Different species use the silk either alone or in composites for a variety of purposes including mechanical reinforcement, thermal regulation, or humidification. The chara...

journal_title：Biopolymers

authors： Sutherland TD,Weisman S,Walker AA,Mudie ST

更新日期：2012-06-01 00:00:00

abstract：:Xylanase XYNII from Trichoderma longibrachiatum is a small protein of the molecular weight 21 kDa, belonging to the family 11 of glycosyl hydrolases, which catalyses hydrolysis of xylan. This article reports thermal stability study of xylanase XYN II conformation in the temperature range 15-65 degrees C by the small a...

journal_title：Biopolymers

authors： Kozak M

更新日期：2006-12-15 00:00:00

abstract：:TACC3 is a centrosomal adaptor protein that plays important roles during mitotic spindle assembly. It interacts with chTOG/XMAP215, which catalyzes the addition of tubulin dimers during microtubule growth. A 3D coiled-coil model for this interaction is available but the structural details are not well described. To ch...

journal_title：Biopolymers

authors： Partida-Hanon A,Treviño MA,Mompeán M,Jiménez MÁ,Bruix M

更新日期：2017-11-01 00:00:00

abstract：:Small-angle x-ray scattering was used for studying intermediate species, isolated after heat-induced aggregation of the A variant of bovine beta-lactoglobulin. The intermediates were separated in two fractions, the heated metastable dimer and heated metastable oligomers larger than the dimer. The pair distance distrib...

journal_title：Biopolymers

authors： Carrotta R,Arleth L,Pedersen JS,Bauer R

更新日期：2003-10-01 00:00:00

abstract：:Bombolitins are five structurally related heptadecapeptides originally isolated from the venom of a bumblebee, acting at membrane level and able to enhance the activity of Phospholipase A2. The biological activity of this class of natural peptides seems to be related to the their ability to form amphiphilic helical st...

journal_title：Biopolymers

authors： Peggion E,Mammi S,Schievano E

更新日期：1997-01-01 00:00:00

abstract：:Water-mediated contacts are known as an important recognition tool in trp-repressor operator systems. One of these contacts involves two conserved base pairs (G(6).C(-6) and A(5). T(-5)) and three amino acids (Lys 72, Ile 79, and Ala 80). To investigate the nature of these contacts, we analyzed the X-ray structure (PD...

journal_title：Biopolymers

authors： Wibowo FR,Rauch C,Trieb M,Wellenzohn B,Liedl KR

更新日期：2004-04-15 00:00:00

abstract：:The natural amino acids are primarily helix breakers at the low assignment temperatures characteristic of many studies, but recent genomic analyses of thermophilic proteins suggest that at high temperatures, some breakers may become strong helix formers. Moreover, the breaker/former inventory has not been previously c...

journal_title：Biopolymers

authors： Nasr KA,Schubert CR,Török M,Kennedy RJ,Kemp DS

更新日期：2009-05-01 00:00:00

abstract：:The rheological characteristics of gastric and duodenal mucin solutions, the building blocks of the mucus layer that covers the epithelia of the two organs, were investigated using particle tracking microrheology. We used biochemically well characterized purified porcine mucins (MUC5AC and MUC2) as models for human mu...

journal_title：Biopolymers

authors： Georgiades P,Pudney PD,Thornton DJ,Waigh TA

更新日期：2014-04-01 00:00:00

abstract：:The structural evolution of regenerated Bombyx mori silk fibroin during shearing with a Couette cell has been studied in situ by synchrotron radiation small- and wide-angle x-ray scattering techniques. An elongation of fibroin molecules was observed with increasing shear rate, followed by an aggregation phase. The agg...

journal_title：Biopolymers

authors： Rössle M,Panine P,Urban VS,Riekel C

更新日期：2004-07-01 00:00:00

abstract：:Bacteria contain a diverse set of RNAs to provide tight regulation of gene expression in response to environmental stimuli. Bacterial small RNAs (sRNAs) work in conjunction with protein cofactors to bind complementary mRNA sequences in the cell, leading to up- or downregulation of protein synthesis. In vivo imaging of...

journal_title：Biopolymers

authors： Kitto RZ,Christiansen KE,Hammond MC

更新日期：2021-01-01 00:00:00

abstract：:The pressure-induced structural changes of a de novo designed four-helix bundle protein, (alpha-l-alpha)(2), in aqueous solution have been investigated by FTIR spectroscopy. Changes in the amide I' band intensity show that pressure induces disruption of tertiary interactions and stabilizes the solvated alpha-helical f...

journal_title：Biopolymers

authors： Takekiyo T,Takeda N,Isogai Y,Kato M,Taniguchi Y

更新日期：2007-02-05 00:00:00

abstract：:A new triclinic crystal structure form of porcine pancreatic procarboxypeptidase B (PCPB) was obtained at higher resolution than the previously known tetragonal crystal structure. This new crystal polymorph has allowed for a corrected, accurate assignment of residues along the polypeptide chain based on the currently ...

journal_title：Biopolymers

authors： Fernández D,Boix E,Pallarès I,Avilés FX,Vendrell J

更新日期：2010-02-01 00:00:00

abstract：:A series of iota-carrageenans containing different amounts of nu-carrageenan (0-23 monomer %) have been prepared from neutrally extracted carrageenan of Eucheuma denticulatum. nu-Carrageenan is the biochemical precursor of iota-carrageenan. The conformational order-disorder transition and rheological properties of the...

journal_title：Biopolymers

authors： van de Velde F,Rollema HS,Grinberg NV,Burova TV,Grinberg VY,Tromp RH

更新日期：2002-11-15 00:00:00

abstract：:Near uv CD spectra of Trp residues in proteins frequently show a complex line shape deriving from the overlap of 1La and 1Lb electronic transitions. This study presents an original empirical method of resolving these components, based on the near uv CD spectra of well-defined complexes of calmodulin domains with targe...

journal_title：Biopolymers

authors： Barth A,Martin SR,Bayley PM

更新日期：1998-06-01 00:00:00

abstract：:The ion atmosphere created by monovalent (Na(+) ) or divalent (Mg(2+) ) cations surrounding a B-form DNA duplex were examined using atomistic molecular dynamics (MD) simulations and the nonlinear Poisson-Boltzmann (PB) equation. The ion distributions predicted by the two methods were compared using plots of radial and...

journal_title：Biopolymers

authors： Robbins TJ,Ziebarth JD,Wang Y

更新日期：2014-08-01 00:00:00

abstract：:In the first two papers of this series, the tools necessary to evaluate substrate ring deformations were developed, and then the modeling of short amylose fragments (maltotriose and maltopentaose) inside the catalytic site of barley alpha-amylase was performed. In this third paper, this docking has been extended to th...

journal_title：Biopolymers

authors： André G,Buléon A,Haser R,Tran V

更新日期：1999-12-01 00:00:00

abstract：:Synthetic RNA poly[r(A-T)] has been synthesized and its CD spectral properties compared to those of poly[r(A-U)], poly[d(A-T)], and poly[d(A-U)] in various salt and ethanolic solutions. The CD spectra of poly[r(A-T)] in an aqueous buffer and of poly[d(A-T)] in 70.8% v/v ethanol are very similar, suggesting that they b...

journal_title：Biopolymers

authors： Vorlícková M,Kypr J,Jovin TM,Planck M

更新日期：1990-02-05 00:00:00

abstract：:Peptides are frequently used model systems for protein folding. They are also gaining increased importance as therapeutics. Here, the ability of molecular dynamics (MD) simulation for describing the structure and dynamics of β-hairpin peptides was investigated, with special attention given to the impact of a single in...

journal_title：Biopolymers

authors： Niebling S,Danelius E,Brath U,Westenhoff S,Erdélyi M

更新日期：2015-11-01 00:00:00

abstract：:The characterization of elastic properties of biopolymers is crucial to understand many molecular reactions determined by conformational bending fluctuations of the polymer. Direct measurement of such elastic properties using single-molecule methods is usually hindered by the intrinsic tendency of such biopolymers to ...

journal_title：Biopolymers

authors： Alemany A,Ritort F

更新日期：2014-12-01 00:00:00

abstract：:DNA sequences containing four types of analog nucleosides are described. All four are pyridine derivatives constructed as C-nucleosides so that they mimic the pyrimidine derivatives 2'-deoxyuridine, thymidine or 2'-deoxycytidine, but in all cases the analogs lack the corresponding O2-carbonyls that in duplex DNA are l...

journal_title：Biopolymers

authors： Sun Z,McLaughlin LW

更新日期：2007-10-05 00:00:00

abstract：:A natural rubber was identified and characterized for the first time in the latex of the perennial Mediterranean shrub Euphorbia characias. Four different methods, i.e., acetone, acetic acid, trichloroacetic acid, and Triton® X-100, followed by successive treatments with cyclohexane/ethanol, were employed to extract t...

journal_title：Biopolymers

authors： Spanò D,Pintus F,Mascia C,Scorciapino MA,Casu M,Floris G,Medda R

更新日期：2012-08-01 00:00:00

abstract：:Flow linear dichroism measurements extended into the vacuum uv region yield inclinations for the base normal from the helix axis of 21 degrees for dA and 40 degrees for dT in the X-form of poly(dAdT).poly(dAdT). These inclination angles are similar to the B form of the synthetic polymer, but the axes around which the ...

journal_title：Biopolymers

authors： Riazance-Lawrence JH,Kang H,Chou PJ,Johnson WC Jr,Vorlícková M

更新日期：1994-11-01 00:00:00

abstract：:To date, several antigens based on the amino-terminal leucine/isoleucine heptad repeat (NHR) region of an HIV-1 envelope protein gp41 and fusion inhibitors based on the carboxy-terminal leucine/isoleucine heptad repeat (CHR) region of gp41 have been reported. We have developed a synthetic antigen targeting the membran...

journal_title：Biopolymers

authors： Nomura W,Mizuguchi T,Tamamura H

更新日期：2016-11-04 00:00:00

abstract：:The interaction of the immunogenic peptide of human hepatitis B virus (HBV) preS(120-145), including B and T epitopes, with phospholipid vesicles has been studied by fluorescence techniques and CD. In addition, interaction of three lipopeptides derived from preS(120-145) containing stearoyl, cholanoyl, and tripalmitoy...

journal_title：Biopolymers

authors： Cajal Y,Rabanal F,Alsina MA,Reig F

更新日期：1996-05-01 00:00:00