Recent advances in DNA catalysis.

abstract：:Recombinant murine amelogenins M179 and M166 were expressed in Escherichia coli and purified. The aggregation properties of these amelogenins have been investigated in aqueous solutions as well as acetonitrile-containing solutions using dynamic light scattering. Dynamic light scattering provides direct measurement of ...

journal_title：Biopolymers

authors： Moradian-Oldak J,Simmer JP,Lau EC,Sarte PE,Slavkin HC,Fincham AG

更新日期：1994-10-01 00:00:00

abstract：:Solid-state nmr spectroscopy provides a robust method for investigating polypeptides that have been prepared by chemical synthesis and that are immobilized by strong interactions with solid surfaces or large macroscopic complexes. Solid-state nmr spectroscopy has been widely used to investigate membrane polypeptides o...

journal_title：Biopolymers

authors： Bechinger B,Kinder R,Helmle M,Vogt TC,Harzer U,Schinzel S

更新日期：1999-01-01 00:00:00

abstract：:Electronic absorbance and fluorescence titrations are used to probe the interaction of chlorobenzylidine with DNA. The binding of chlorobenzylidine to DNA results in hypochromism, a small shift to a longer wavelength in the absorption spectra, and emission quenching in the fluorescence spectra. These spectral characte...

journal_title：Biopolymers

authors： Zhong W,Yu JS,Huang W,Ni K,Liang Y

更新日期：2001-01-01 00:00:00

abstract：:Cellulose acetate (DS = 2.45) was extensively investigated by Circular Dichroism (CD) in acetonitrile and dioxane. We found great differences between the CD spectra of a 1 wt % acetonitrile solution and the corresponding dioxane solution of cellulose acetate (CA) indicating that the macromolecules exist in those solut...

journal_title：Biopolymers

authors： Meissner D,Einfeldt J,Schareina T

更新日期：1999-08-01 00:00:00

abstract：:Individual ethidium-stained DNA molecules, embedded in an agarose gel made with electrophoresis buffer (0.05 molar salt), are observed using a fluorescence microscope. In the first experiment, open circular 66 kilobase pair (kbp) plasmids, immobilized by agarose fibers threaded through their centers, display entropic ...

journal_title：Biopolymers

authors： Smith SB,Bendich AJ

更新日期：1990-07-01 00:00:00

abstract：:In this paper, we explore the possibility of using experimental observations in the Monte Carlo chain growth method that we have previously developed. In this method, the macromolecule (peptide, protein, nuclei acid, etc.) is grown atom-by-atom (or residue-by-residue, etc.) and partial chains are replicated according ...

journal_title：Biopolymers

authors： Bascle J,Garel T,Orland H,Velikson B

更新日期：1993-12-01 00:00:00

abstract：:Insect neuropeptides of the insect kinin class share a common C-terminal pentapeptide sequence F(1)X(1)(2)X(2)(3)W(4)G(5)-NH(2) (X(2)(3) = P, S, A) and regulate such critical physiological processes as water balance and digestive enzyme release. Analogs of the insect kinin class, in which the critical residues of F(1)...

journal_title：Biopolymers

authors： Zubrzak P,Williams H,Coast GM,Isaac RE,Reyes-Rangel G,Juaristi E,Zabrocki J,Nachman RJ

更新日期：2007-01-01 00:00:00

abstract：:In order to check our current knowledge on the principles involved in beta-hairpin formation, we have modified the sequence of a 3:5 beta-hairpin forming peptide with two different purposes, first to increase the stability of the formed 3:5 beta-hairpin, and second to convert the 3:5 beta-hairpin into a 2:2 beta-hairp...

journal_title：Biopolymers

authors： Santiveri CM,Pantoja-Uceda D,Rico M,Jiménez MA

更新日期：2005-10-15 00:00:00

abstract：:The advent of kinetically controlled Native Chemical Ligation has permitted more efficient sequential Native Chemical Ligation reactions to take place in one pot where one or more of the peptide fragments contains an N-terminal cysteine residue and a C-terminal thioester. The reactivity of the thioester component can ...

journal_title：Biopolymers

authors： Ackrill T,Anderson DW,Macmillan D

更新日期：2010-01-01 00:00:00

abstract：:H-Pro-Pro-Asp-NH2 is a highly active and selective catalyst for asymmetric aldol reactions. Here, the versatility of H-Pro-Pro-Asp-NH2 has been further improved by immobilization on a solid support and functionalization with a short polyethylene glycol linker at the C-terminus. The development, synthesis, and the cata...

journal_title：Biopolymers

authors： Revell JD,Gantenbein D,Krattiger P,Wennemers H

更新日期：2006-01-01 00:00:00

abstract：:The spectroscopic properties of syringomycin E, an antibiotic lipodepsinonapeptide associated with pathological states in plants, have been investigated by uv absorbance and CD spectroscopies, and by the synthesis of relevant model compounds. Initial studies [E. Vaillo, A. Ballio, P. L. Luisi, and R. M. Thomas (1990) ...

journal_title：Biopolymers

authors： Vaillo E,Ballio A,Luisi PL,Thomas RM

更新日期：1992-10-01 00:00:00

abstract：:The creation of peptide libraries by site-selective modification of a few peptide substrates would increase the efficiency of discovery processes, but still is a real synthetic challenge. The site-selective modification of small peptides at serine or threonine residues, by using a short scission-addition procedure, al...

journal_title：Biopolymers

authors： Romero-Estudillo I,Saavedra C,Boto A,Álvarez E

更新日期：2015-09-01 00:00:00

abstract：:The solution conformations of a hybrid sequence peptide related to the bee venom peptide apamin have been determined using two-dimensional 1H-nmr. Apamin is an 18 amino acid peptide containing a C-terminal helix that is stabilized by two disulfide bonds. The deletion of one residue (K4) of the N-terminal "scaffold" re...

journal_title：Biopolymers

authors： Volkman BF,Wemmer DE

更新日期：1997-04-05 00:00:00

abstract：:Three new polyoxaalkyl esters of lasalocid are synthesized. Their ability to form complexes with Li(+) and Na(+) cations is studied using multinuclear NMR methods, FTIR spectroscopy in the middle and far IR regions, and mass spectrometry. It is found that lasalocid esters form only 1:1 complexes with the metal cations...

journal_title：Biopolymers

authors： Pankiewicz R,Schroeder G,Gierczyk B,Brzezinski B,Bartl F

更新日期：2002-10-15 00:00:00

abstract：:Structural characterization of G protein-coupled receptors (GPCRs) is hindered by the inherent hydrophobicity, flexibility, and large size of these signaling proteins. Insights into conformational preferences and the three-dimensional (3D) structure of domains of these receptors can be obtained using polypeptide fragm...

journal_title：Biopolymers

authors： Caroccia KE,Estephan R,Cohen LS,Arshava B,Hauser M,Zerbe O,Becker JM,Naider F

更新日期：2011-01-01 00:00:00

abstract：:MUC1 mucin is a large transmembrane glycoprotein whose extracelluler domain is composed of repeating units of a 20 amino acid sequence. In the cancer associated state, this protein expression becomes upregulated and underglycosylated. Previous studies, which show an enhanced binding of a 5-repeat over a 1-repeat MUC1 ...

journal_title：Biopolymers

authors： Schuman JT,Grinstead JS,Apostolopoulos V,Campbell AP

更新日期：2005-02-05 00:00:00

abstract：:Serine palmitoyltransferase (SPT) catalyses the first step in the de novo biosynthesis of sphingolipids (SLs). It uses a decarboxylative Claisen-like condensation reaction to couple L-serine with palmitoyl-CoA to generate a long-chain base product, 3-ketodihydrosphingosine. SLs are produced by mammals, plants, yeast, ...

journal_title：Biopolymers

authors： Raman MC,Johnson KA,Clarke DJ,Naismith JH,Campopiano DJ

更新日期：2010-09-01 00:00:00

abstract：:The phase content of amylose-water mixtures (0.7/0.3 w/w) has been analyzed by transmission Fourier transform infrared (FTIR) spectroscopy in the 1175-950 cm(-1) region. Spectra are obtained under three different conditions: (a) as a function of temperature (T) from 25 to 97 degrees C; (b) at room temperature (RT), af...

journal_title：Biopolymers

authors： Bernazzani P,Chapados C,Delmas G

更新日期：2001-03-01 00:00:00

abstract：:The thermodynamics of the stacking to unstacking transitions of 24 single-stranded DNA sequences (ssDNA), 10-12 bases in length, in sodium phosphate buffer were determined from 10 to 95 degrees C, using differential scanning calorimetry (DSC). An additional 22 ssDNA sequences did not exhibit an S<=>U transition in thi...

journal_title：Biopolymers

authors： Ramprakash J,Lang B,Schwarz FP

更新日期：2008-11-01 00:00:00

abstract：:Experimental and computational methods of infrared microspectroscopy (IRI-MSP) and infrared spectral mapping (ISM) are presented. These methods are subsequently applied to the analysis of cirrhotic liver tissue. The sensitivity of infrared spectral mapping toward spectral changes caused by disease will be demonstrated...

journal_title：Biopolymers

authors： Diem M,Chiriboga L,Yee H

更新日期：2000-01-01 00:00:00

abstract：:The enhanced nucleophilicity and redox sensitivity of the thiol group renders cysteine residues susceptible to numerous electrophilic and oxidative post-translational modifications to form disulfides, sulfenic acids, nitrosothiols, and lipid-modified species. Outside of these well-characterized modifications of cystei...

journal_title：Biopolymers

authors： Shannon DA,Weerapana E

更新日期：2014-02-01 00:00:00

abstract：:Cyclotides are disulfide-rich miniproteins with a circular backbone and a knotted arrangement ofdisulfide bonds. Because these plant-derived peptides are resistant to degradation and exhibit a diverse range of bioactivity they have become important agronomic and industrial objectives. They belong to a group of compoun...

journal_title：Biopolymers

authors： Dörnenburg H

更新日期：2010-01-01 00:00:00

abstract：:The secondary structure of a 38 kDa core protein from pig skin proteodermatan sulfate (PDS), was investigated in solution using CD and Fourier transform (FT) ir spectroscopy. Both techniques generally have provided complementary data on the secondary structures of proteins. CD spectral analysis has shown that the core...

journal_title：Biopolymers

authors： Renugopalakrishnan V,Damle SP,Horowitz PM,Moore S,Hutson TB,Gregory JD

更新日期：1989-11-01 00:00:00

abstract：:It was shown earlier, from database analysis, model building studies, and molecular dynamics simulations that formation of cross-strand bifurcated or Extra Watson-Crick hydrogen (EWC) bonds between successive base pairs may lead to extra rigidity to DNA double helices of certain sequences. The strengths of these hydro...

journal_title：Biopolymers

authors： Bandyopadhyay D,Bhattacharyya D

更新日期：2006-10-15 00:00:00

abstract：:Hydrophobicity has proven to be an extremely useful parameter in small molecule drug discovery programmes given that it can be used as a predictive tool to enable rational design. For larger molecules, including peptoids, where folding is possible, the situation is more complicated and the average hydrophobicity (as d...

journal_title：Biopolymers

authors： Bolt HL,Williams CEJ,Brooks RV,Zuckermann RN,Cobb SL,Bromley EHC

更新日期：2017-07-01 00:00:00

abstract：:The discovery of extreme halophile microorganisms in the Dead Sea, which are specifically dependent on a multimolar salt environment to survive, stimulated major developments in biology and physical chemistry. The minireview focuses on the molecular level. After a brief introduction to the history of halophile studies...

journal_title：Biopolymers

authors： Zaccai G

更新日期：2013-04-01 00:00:00

abstract：:A secondary structure sequence is a symbolic string composed of three kinds of letters, indicating the helix, strand, and coil (including turns), respectively. A graphic representation for this abstract symbolic sequence is proposed here, called the S curve. The S curve is the unique representation for a given seconda...

journal_title：Biopolymers

authors： Zhang CT,Zhang R

更新日期：2000-06-01 00:00:00

abstract：:The crystal and molecular structure of the pentapeptide Boc-D-Ala-delta Phe-Gly-delta Phe-D-Ala-OMe, containing two dehydrophenylalanine residues, was determined by x-ray diffraction. The molecule crystallizes in the orthorombic P2(1)2(1)2(1) space group, with a = 10.439(3), b = 15.319(3) and c = 21.099(4) A. In the s...

journal_title：Biopolymers

authors： Ciajolo MR,Tuzi A,Pratesi CR,Fissi A,Pieroni O

更新日期：1990-01-01 00:00:00

abstract：:The conformation of basic fatty acid binding protein from chicken liver and the binding properties of the apo protein toward 11-dansylamino-undecanoic acid were investigated by CD and fluorescence spectroscopy. In one set of experiments the binding process was followed by the appearance of induced optical activity in ...

journal_title：Biopolymers

authors： Schievano E,Quarzago D,Spadon P,Monaco HL,Zanotti G,Peggion E

更新日期：1994-07-01 00:00:00

abstract：:Purified integral membrane proteins require amphipathic molecules to maintain their solubility in aqueous solutions. These complexes, in turn, are used in studies to characterise the protein structures by a variety of biophysical and structural techniques, including spectroscopy, crystallography, and cryo-electron mic...

journal_title：Biopolymers

authors： Ireland SM,Sula A,Wallace BA

更新日期：2018-08-01 00:00:00