Cyclotide synthesis and supply: from plant to bioprocess.

abstract：:Apelin is the endogenous ligand of APJ, which belongs to the superfamily of G protein-coupled receptors. In recent years, the apelin/APJ system has been detected in many tissues and emerges as a promising target for the treatment of various pathophysiological conditions. Pyr1-apelin-13 is the major isoform of apelin i...

journal_title：Biopolymers

authors： Murza A,Belleville K,Longpré JM,Sarret P,Marsault É

更新日期：2014-07-01 00:00:00

abstract：:Water-mediated contacts are known as an important recognition tool in trp-repressor operator systems. One of these contacts involves two conserved base pairs (G(6).C(-6) and A(5). T(-5)) and three amino acids (Lys 72, Ile 79, and Ala 80). To investigate the nature of these contacts, we analyzed the X-ray structure (PD...

journal_title：Biopolymers

authors： Wibowo FR,Rauch C,Trieb M,Wellenzohn B,Liedl KR

更新日期：2004-04-15 00:00:00

abstract：:The kinetics of the hydrodynamic volume change associated with the unfolding and refolding of a globular protein can be observed using high performance size exclusion chromatography. Chromatographic profiles that evidence such dynamics can be simulated using equations in which chromatographic partitioning and the conf...

journal_title：Biopolymers

authors： Shalongo W,Jagannadham M,Stellwagen E

更新日期：1993-01-01 00:00:00

abstract：:Small multidisulfide-containing proteins are attractive structural templates to produce a biologically active conformation that mimics the binding surface of natural large proteins. In particular, the structural motif that is evolutionary conserved in all scorpion toxins has a small size (30-40 amino acid residues), a...

journal_title：Biopolymers

authors： Vita C,Vizzavona J,Drakopoulou E,Zinn-Justin S,Gilquin B,Ménez A

更新日期：1998-01-01 00:00:00

abstract：:A simple solid-state NMR method was used to study the structure of (13)C- and (15)N-enriched silk from two Australian orb-web spider species, Nephila edulis and Argiope keyserlingi. Carbon-13 and (15)N spectra from alanine- or glycine-labeled oriented dragline silks were acquired with the fiber axis aligned parallel o...

journal_title：Biopolymers

authors： Bonev B,Grieve S,Herberstein ME,Kishore AI,Watts A,Separovic F

更新日期：2006-06-05 00:00:00

abstract：:Partially acetylated, high molecular weight glucuronans were produced by a Sinorhizobium meliloti mutant strain. Two native glucuronan samples with various degrees of acetylation were sonicated to obtain lower molecular weight samples and with low viscosity suitable for chemical modification and (13)C NMR experiments....

journal_title：Biopolymers

authors： Pau-Roblot C,Petit E,Sarazin C,Courtois J,Courtois B,Barbotin JN,Séguin JP

更新日期：2002-06-01 00:00:00

abstract：:Studies of catalytically active DNA sequences have expanded considerably since the first artificial deoxyribozyme was identified in 1994. Nevertheless, the field is still quite young, and advances in both fundamental understanding and practical applications of deoxyribozymes are still developing. Deoxyribozymes that e...

journal_title：Biopolymers

authors： Höbartner C,Silverman SK

更新日期：2007-12-05 00:00:00

abstract：:The linear viscoelastic and rheological properties of high molecular weight ovine submaxillary mucin (OSM) solution have been investigated in terms of the Newtonian steady-flow viscosity [eta(gamma)], the complex oscillatory viscosity [eta*(omega)], and the storage and loss shear moduli [G'(omega) and G"(omega)]. It w...

journal_title：Biopolymers

authors： Soby LM,Jamieson AM,Blackwell J,Jentoft N

更新日期：1990-08-15 00:00:00

abstract：:The advent of kinetically controlled Native Chemical Ligation has permitted more efficient sequential Native Chemical Ligation reactions to take place in one pot where one or more of the peptide fragments contains an N-terminal cysteine residue and a C-terminal thioester. The reactivity of the thioester component can ...

journal_title：Biopolymers

authors： Ackrill T,Anderson DW,Macmillan D

更新日期：2010-01-01 00:00:00

abstract：:The aim of this study was to develop a nondestructive method to quantitate relative amounts of n-3 and n-6 polyunsaturated fatty acid (PUFA) species in vegetable oils and oil seeds using Fourier transform IR spectroscopy (FTIR). The alkene Cbond;H stretching vibrations of unsaturated fatty acids in oils showed IR abso...

journal_title：Biopolymers

authors： Yoshida S,Yoshida H

更新日期：2003-12-01 00:00:00

abstract：:The indirect solvent-induced effect on the free energy of binding of biopolymers is examined within the framework of classical statistical mechanics. We focus specifically on the role of the solute-solvent hydrogen bonding. In particular, we have estimated the first order solvent effect on the indirect interaction bet...

journal_title：Biopolymers

authors： Ben-Naim A,Ting KL,Jernigan RL

更新日期：1990-05-01 00:00:00

abstract：:The rheological characteristics of gastric and duodenal mucin solutions, the building blocks of the mucus layer that covers the epithelia of the two organs, were investigated using particle tracking microrheology. We used biochemically well characterized purified porcine mucins (MUC5AC and MUC2) as models for human mu...

journal_title：Biopolymers

authors： Georgiades P,Pudney PD,Thornton DJ,Waigh TA

更新日期：2014-04-01 00:00:00

abstract：:Peptomers are oligomeric molecules composed of both α-amino acids and N-substituted glycine monomers, thus creating a hybrid of peptide and peptoid units. Peptomers have been used in several applications such as antimicrobials, protease inhibitors, and antibody mimics. Despite the considerable promise of peptomers as ...

journal_title：Biopolymers

authors： Butterfoss GL,Drew K,Renfrew PD,Kirshenbaum K,Bonneau R

更新日期：2014-09-01 00:00:00

abstract：:Intermolecular NOE experiments have been used to explore interactions of water and ethanol molecules in 35% ethanol/65% water (v/v) with the peptide Trp-cage at temperatures from 5 to 25 degrees C. Magnetic dipole-dipole cross-relaxation terms sigma(HH) (NOE) and sigma(HH) (ROE) for interaction of solvent components w...

journal_title：Biopolymers

authors： Neuman RC Jr,Gerig JT

更新日期：2008-10-01 00:00:00

abstract：:A novel nucleic acid model using peptide ribonucleic acid (PRNA), which contains 5'-amino-5'-deoxypyrimidine ribonucleoside as a recognition site for nucleic acids, has been designed, synthesized and applied to the external reversible control of recognition behavior of the complementary polynucleotide through the orie...

journal_title：Biopolymers

authors： Wada T,Sato H,Inoue Y

更新日期：2004-01-01 00:00:00

abstract：:The periodical D-band pattern is generally considered a unique ultrastructural feature shared by all fibril-forming collagens, which correlates with the intrafibril, paracrystalline array of tropocollagen monomers. Distinct band patterns have been reported, however, for collagen stained long-spacing (SLS) crystallites...

journal_title：Biopolymers

authors： Ortolani F,Giordano M,Marchini M

更新日期：2000-11-01 00:00:00

abstract：:Optical melting curves of 22 DNA dumbbells with the 16-base pair duplex sequence 5'-G-C-A-T-C-A-T-C-G-A-T-G-A-T-G-C-3' linked on both ends by single-strand loops of A, or C, sequences (iota = 2, 3, 4, 6, 8, 10, 14). T sequences (iota = 2, 3, 4, 6, 8, 10), and G iota sequences (iota = 2, 4) were measured in phosphate b...

journal_title：Biopolymers

authors： Paner TM,Riccelli PV,Owczarzy R,Benight AS

更新日期：1996-12-01 00:00:00

abstract：:We have found that tri-L-alanine (Ala3) can crystallize in a parallel-chain beta structure in addition to the previously known antiparallel-chain beta structure. Although the chain conformations in each structure are essentially similar, the ir and Raman spectra are distinctively different. We have calculated the norm...

journal_title：Biopolymers

authors： Qian W,Bandekar J,Krimm S

更新日期：1991-02-05 00:00:00

abstract：:The protected tripeptide tert-butyloxycarbonyl-L-leucyl-L-aminosuccinyl-L-phenylalaninamide crystallizes in the orthorhombic space group P2(1)2(1)2(1), with a = 6.214(3), b = 12.832(3), c = 33.094(4) A, Z = 4. The structure was solved by direct methods using MULTAN 80 and refined to an R value of 0.055 for 1458 reflec...

journal_title：Biopolymers

authors： Capasso S,Mazzarella L,Sica F,Zagari A

更新日期：1989-01-01 00:00:00

abstract：:Lung cancer is usually fatal once it becomes metastatic. However, in order to develop metastases, a tumor usually invades the basal membrane and enters the vascular or lymphatic system. In this study, a three-dimensional artificial membrane using collagen type I, one of the main components of basal membranes, was esta...

journal_title：Biopolymers

authors： Yang Y,Sulé-Suso J,Sockalingum GD,Kegelaer G,Manfait M,El Haj AJ

更新日期：2005-08-15 00:00:00

abstract：:Laminins are a multifunctional molecule with numerous active sites that have been identified in short peptide sequences. Mixed peptide-conjugated chitosan membranes using laminin-derived active peptides have been previously demonstrated to be useful as a biomaterial for tissue engineering. In this study, two syndecan-...

journal_title：Biopolymers

authors： Otagiri D,Yamada Y,Hozumi K,Katagiri F,Kikkawa Y,Nomizu M

更新日期：2013-11-01 00:00:00

abstract：:A series of CD measurements were carried out on members of peptides in the endothelin and sarafotoxin families. The helical structures taken by these peptides containing the helical motif with the sequences of Cys-X-X-X-Cys and Cys-X-Cys [Y. Kobayashi et al. (1991) Neurochemistry International Vol. 18, pp. 525-534] ar...

journal_title：Biopolymers

authors： Tamaoki H,Kyogoku Y,Nakajima K,Sakakibara S,Hayashi M,Kobayashi Y

更新日期：1992-04-01 00:00:00

abstract：:Depsipeptide analogues of peptide sequences can help in elucidating the role of specific hydrogen bonds in determining the conformation in peptides. The repeating pentapeptide and hexapeptide sequences of elastin have been suggested to contain a type II beta-turn with a 4----1 hydrogen bond. Depsipeptide analogues of ...

journal_title：Biopolymers

authors： Arad O,Goodman M

更新日期：1990-10-01 00:00:00

abstract：:Circular dichroic spectra and oscillator strengths of the π-π transition near 190 nm are calculated for helical (Gly)6 and (Ala)6 at 30° intervals of the backbone torsion angles (ϕ,ψ) over the range -180° ≤ ϕ ≤ -60°, -60° ≤ ψ ≤ 180°, using the partially dispersive normal mode treatment of the dipole interaction model....

journal_title：Biopolymers

authors： Applequist J

更新日期：1981-02-01 00:00:00

abstract：:The transient electric birefringence of two small DNA restriction fragments of the same molecular weight, one of which migrates anomalously slowly on polyacrylamide gels, has been investigated. Both fragments exhibit negative birefringence. The decay of the birefringence of the anomalously slowly migrating fragment is...

journal_title：Biopolymers

authors： Stellwagen NC

更新日期：1991-11-01 00:00:00

abstract：:Conformational analysis of the neurohypophyseal hormones oxytocin (OT) and arginine-vasopressin (AVP) has been carried out using two different computational approaches and three force fields, namely by the Electrostatically Driven Monte Carlo (EDMC) method, with the Empirical Conformational Energy Program for Peptides...

journal_title：Biopolymers

authors： Liwo A,Tempczyk A,Ołdziej S,Shenderovich MD,Hruby VJ,Talluri S,Ciarkowski J,Kasprzykowski F,Lankiewicz L,Grzonka Z

更新日期：1996-02-01 00:00:00

abstract：:The aminoacyl-tRNA synthetases are an ancient group of enzymes that catalyze the covalent attachment of an amino acid to its cognate transfer RNA. The question of specificity, that is, how each synthetase selects the correct individual or isoacceptor set of tRNAs for each amino acid, has been referred to as the second...

journal_title：Biopolymers

authors： Beuning PJ,Musier-Forsyth K

更新日期：1999-01-01 00:00:00

abstract：:We review the neighbor exclusion model and some of its applications to analyze the binding data of DNA-ligand complexes. We revisit the closed form of the model developed by McGhee and von Hippel in 1974, showing that this classic model can be used to help studying the behavior of DNA contour and persistence lengths w...

journal_title：Biopolymers

authors： Rocha MS

更新日期：2010-01-01 00:00:00

abstract：:A theoretical study is presented of the influence of salt bridges between cationic side chains and DNA phosphates on DNA conformation and flexibility. The DNA sequence studied is that of the catabolite activator protein binding oligomer from the crystallized complex. The effect of salt bridges is modeled by neutraliza...

journal_title：Biopolymers

authors： Gurlie R,Duong TH,Zakrzewska K

更新日期：1999-04-01 00:00:00

abstract：:The de novo design of peptides and proteins has emerged as an approach for investigating protein structure and function. The success relies heavily on the ability to design relatively short peptides that can adopt stable secondary structures. To this end, substitution with α,β-dehydroamino acids, especially α,β-didehy...

journal_title：Biopolymers

authors： Gupta M,Chauhan VS

更新日期：2011-03-01 00:00:00