Abstract:
:MUC1 mucin is a large transmembrane glycoprotein whose extracelluler domain is composed of repeating units of a 20 amino acid sequence. In the cancer associated state, this protein expression becomes upregulated and underglycosylated. Previous studies, which show an enhanced binding of a 5-repeat over a 1-repeat MUC1 peptide to a panel of anti-MUC1 antibodies, have led us to investigate the structural and dynamic consequences of increasing repeat number. Two MUC1 peptides were studied: a 16mer corresponding to slightly less than one full repeat of the MUC1 tandem repeat sequence (GVTSAPDTRPAPGSTA) and a 40mer corresponding to two full repeats of the MUC1 sequence (VTSAPDTRPAPGSTAPPAHG)2. Isotopically labeled versions of these MUC1 peptides were cloned, expressed, purified, and evaluated structurally and dynamically using 15N- and 13C-edited NMR approaches. The data show that MUC1 structure, dynamics, and antibody binding affinity are invariant with increasing repeat number. In light of these results, we conclude that the enhanced antibody affinity of the 5-repeat over the 1-repeat MUC1 peptide is due to multivalency effects, and not due to the development of higher order structure in the longer length peptides. The implications of these results are discussed within the context of a multiple repeat MUC1 breast cancer vaccine design.
journal_name
Biopolymersjournal_title
Biopolymersauthors
Schuman JT,Grinstead JS,Apostolopoulos V,Campbell APdoi
10.1002/bip.20190subject
Has Abstractpub_date
2005-02-05 00:00:00pages
107-20issue
2eissn
0006-3525issn
1097-0282journal_volume
77pub_type
杂志文章相关文献
BIOPOLYMERS文献大全abstract::Molecular dynamics (MD) simulations on stem loop 2 of U1 small nuclear RNA and a construct of the U1A protein were carried out to obtain predictions of the structures for the unbound forms in solution and to elucidate dynamical aspects of induced fit upon binding. A crystal structure of the complex between the U1A pro...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10251
更新日期:2002-12-15 00:00:00
abstract::This brief account traces the development of a "competition dialysis" method used to characterize the structural and sequence selectivity of DNA binding compounds. The method was inspired by a simple "differential dialysis" method pioneered by Don Crothers to explore base-selective intercalator binding. Results from c...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22660
更新日期:2015-09-01 00:00:00
abstract::The DNA-binding protein HU from Bacillus stearothermophilus (HUBst) is a dimer with a molecular weight of 195 kDa that is capable of bending DNA. An x-ray structure has been determined previously [Tanaka et al. 1984) Nature, vol. 310, pp. 376-381], but no structure could be established for a large part of the supposed...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(sici)1097-0282(1996)40:5<553::aid-bip13>3
更新日期:1996-01-01 00:00:00
abstract::Methods to generate fibers from hydrogels, with control over mechanical properties, fiber diameter, and crystallinity, while retaining cytocompatibility and degradability, would expand options for biomaterials. Here, we exploited features of silk fibroin protein for the formation of tunable silk hydrogel fibers. The b...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.23030
更新日期:2017-09-01 00:00:00
abstract::Human islet amyloid polypeptide (hIAPP), or amylin, is a 37 amino acid hormone secreted by pancreatic beta-cells. hIAPP constitutes approximately 90% of the amyloid deposits found in type II diabetic patients. It has been shown that the central region of the peptide (hIAPP(20-29)) constitutes the nucleation site for t...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10305
更新日期:2003-05-01 00:00:00
abstract::Among the most exciting functional features of G-protein coupled receptors (GPCRs) that are coming into focus lately are those relating to the role and structural characteristics of their oligomerization (mostly homo- and heterodimers). The structural underpinnings of these novel functional insights are still not clea...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10311
更新日期:2002-01-01 00:00:00
abstract::The kinetics of the hydrodynamic volume change associated with the unfolding and refolding of a globular protein can be observed using high performance size exclusion chromatography. Chromatographic profiles that evidence such dynamics can be simulated using equations in which chromatographic partitioning and the conf...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360330113
更新日期:1993-01-01 00:00:00
abstract::Regulatory, receptor-binding peptides are considered as the agents of choice for diagnostic imaging and therapy of cancers, because their receptors are overexpressed in various human cancer cells. It has been recently indicated that there is a putative role of NPY in breast tumors. The expression of the two best-inves...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20666
更新日期:2007-01-01 00:00:00
abstract::The X-ray diffraction analysis of a stereocontrolled heterochiral designed model peptide Boc-(D) Pro-Thr-OMe (1) revealed the existence of an unusual folded molecular structure, stabilized via an effective unconventional C---H…O type intramolecular hydrogen-bond, encompassing a noncovalent 12-membered ring-motif. Toge...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21705
更新日期:2012-01-01 00:00:00
abstract::Water-mediated contacts are known as an important recognition tool in trp-repressor operator systems. One of these contacts involves two conserved base pairs (G(6).C(-6) and A(5). T(-5)) and three amino acids (Lys 72, Ile 79, and Ala 80). To investigate the nature of these contacts, we analyzed the X-ray structure (PD...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20023
更新日期:2004-04-15 00:00:00
abstract::Calcium ions (Ca(2+) ) play key roles in various fundamental biological processes such as cell signaling and brain function. Molecular dynamics (MD) simulations have been used to study such interactions, however, the accuracy of the Ca(2+) models provided by the standard MD force fields has not been rigorously tested....
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22868
更新日期:2016-10-01 00:00:00
abstract::Two-dimensional nmr spectra [correlated spectroscopy (COSY), homonuclear Hartmann-Hahn (HOHAHA), nuclear Overhauser effect spectroscopy (NOESY)] have been observed for cross-linked lysozyme, a chemically modified lysozyme derivative with an extra ester cross-link between residues E35 and W108. Eight shifted cross-peak...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0282(199702)41:2<131::AID-BIP2>
更新日期:1997-02-01 00:00:00
abstract::This work concerns the aggregation properties of (Gly-Val-Gly-Val-Pro)(251) rec, a polypentapeptide reflecting a highly conserved repetitive unit of the bioelastomer, elastin. On raising the temperature of aqueous solutions above 25 degrees C, this polypeptide was already known to undergo concurrent conformational cha...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/1097-0282(200107)59:1<51::AID-BIP1005>3.0.
更新日期:2001-07-01 00:00:00
abstract::The constraints on combinations of nearest neighbors in nucleic acid sequences and the numbers of independent sequences needed to describe nearest-neighbor properties of oligomers and polymers are derived and summarized. It has been pointed out in previous work [D. M. Gray and I. Tinoco, Jr. (1970) Biopolymers, Vol. 9...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(sici)1097-0282(199712)42:7<783::aid-bip4>
更新日期:1997-12-01 00:00:00
abstract::The pressure-induced structural changes of a de novo designed four-helix bundle protein, (alpha-l-alpha)(2), in aqueous solution have been investigated by FTIR spectroscopy. Changes in the amide I' band intensity show that pressure induces disruption of tertiary interactions and stabilizes the solvated alpha-helical f...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20628
更新日期:2007-02-05 00:00:00
abstract::Gold nanoparticles are known to aggregate in the presence of proper multifunctional compounds. For those larger than 3 nm, the process can be followed with the naked eye because the surface plasmon absorption band of the particles shifts to longer wavelengths and the solution color changes from red to blue. To exploit...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.23111
更新日期:2018-08-01 00:00:00
abstract::The discovery of extreme halophile microorganisms in the Dead Sea, which are specifically dependent on a multimolar salt environment to survive, stimulated major developments in biology and physical chemistry. The minireview focuses on the molecular level. After a brief introduction to the history of halophile studies...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22154
更新日期:2013-04-01 00:00:00
abstract::Late expression factor 4 (LEF4) is one of the four subunits of Autographa californica nuclear polyhedrosis virus (AcNPV) RNA polymerase. LEF4 was overexpressed in Escherichia coli and recombinant protein was subjected to structural characterization. Chemical induced unfolding of LEF4 was investigated using intrinsic f...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21180
更新日期:2009-07-01 00:00:00
abstract::Electronic absorbance and fluorescence titrations are used to probe the interaction of chlorobenzylidine with DNA. The binding of chlorobenzylidine to DNA results in hypochromism, a small shift to a longer wavelength in the absorption spectra, and emission quenching in the fluorescence spectra. These spectral characte...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10001
更新日期:2001-01-01 00:00:00
abstract::A conformational study of poly-L-serine has shown that it can exist in the left-handed α-helical form. A study of a pair of peptide units with the serine sidegroup attached to the α carbon atom linking the two units showed that OH ⃛O hydrogen bonds between the OH group of the side chain and a carbonyl oxygen of the f...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.1968.360060403
更新日期:1968-04-01 00:00:00
abstract::The solution structure of a hexapeptide, cyclo (Gln-Trp-Phe-Gly-Leu-Met), which is a selective NK-2 antagonist, has been studied by a combination of two-dimensional nmr and molecular dynamics (MD) techniques. The simulation based on nmr and MD data resulted in the convergence to a family of structures. Free molecular ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360340906
更新日期:1994-09-01 00:00:00
abstract::Seven water-insoluble (1 --> 3)-beta-D-glucan fractions TM8-1 to TM8-7 with weight-average molecular mass M(w) ranged from 2.22 to 77.4 x 10(4) obtained from the sclerotia of Pleurotus tuber-regium were carboxymethylated to produce the water-soluble fractions CTM8-1 to CTM8-7 with M(w) ranged from 3.87 to 87.8 x 10(4)...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10277
更新日期:2003-02-01 00:00:00
abstract::G protein-coupled receptor kinase 2 (GRK2) plays a central role in the cellular transduction network. In particular, during chronic heart failure GRK2 is upregulated and believed to contribute to disease progression. Thereby, its inhibition offers a potential therapeutic solution to several pathological conditions. In...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22295
更新日期:2014-01-01 00:00:00
abstract::IR vibrational CD (VCD) has been observed for the cyclic pentapeptide cyclo-(-Gly-Pro-Gly-D-Ala-Pro-) in solution in CDBr3. The observed VCD spectra do not resemble the VCD features of any of the previously reported peptide secondary structures, such as alpha-helical, "random coil," or sheet structures, and might be d...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360320912
更新日期:1992-09-01 00:00:00
abstract::The periodical D-band pattern is generally considered a unique ultrastructural feature shared by all fibril-forming collagens, which correlates with the intrafibril, paracrystalline array of tropocollagen monomers. Distinct band patterns have been reported, however, for collagen stained long-spacing (SLS) crystallites...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/1097-0282(200011)54:6<448::AID-BIP80>3.0.C
更新日期:2000-11-01 00:00:00
abstract::The advent of kinetically controlled Native Chemical Ligation has permitted more efficient sequential Native Chemical Ligation reactions to take place in one pot where one or more of the peptide fragments contains an N-terminal cysteine residue and a C-terminal thioester. The reactivity of the thioester component can ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21473
更新日期:2010-01-01 00:00:00
abstract::The solution conformations of three trispeptides--L,L,L-1,3,5-C6H3[CH2NHCOCH(X)-NHBoc++ +]3, X = CH3 (Ala) or CH2CH(CH3)2 (Leu), and L,L,L-N(CH2CH2NHCOCH[CH2-CH(CH3)2]NHBoc)3--have been determined from their ir and vibrational CD (VCD) spectra in the NH stretching and carbonyl stretching regions in apolar solution. Th...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360320706
更新日期:1992-07-01 00:00:00
abstract::Insect neuropeptides of the insect kinin class share a common C-terminal pentapeptide sequence F(1)X(1)(2)X(2)(3)W(4)G(5)-NH(2) (X(2)(3) = P, S, A) and regulate such critical physiological processes as water balance and digestive enzyme release. Analogs of the insect kinin class, in which the critical residues of F(1)...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20638
更新日期:2007-01-01 00:00:00
abstract::The magnitude and nature of lesion-induced energetic perturbations empirically correlate with mutagenicity/cytotoxicity profiles and can be predictive of lesion outcomes during polymerase-mediated replication in vitro. In this study, we assess the sequence and counterbase-dependent energetic impact of the Thymine glyc...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22680
更新日期:2015-09-01 00:00:00
abstract::Bombolitins are five structurally related heptadecapeptides originally isolated from the venom of a bumblebee, acting at membrane level and able to enhance the activity of Phospholipase A2. The biological activity of this class of natural peptides seems to be related to the their ability to form amphiphilic helical st...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0282(1997)43:6<419::AID-BIP4>3.
更新日期:1997-01-01 00:00:00