Conformational preferences of the amylin nucleation site in SDS micelles: an NMR study.

abstract：:The common blue marine mussel adheres to underwater surfaces using an adhesive protein (Mefp-1) extruded from its foot. This highly hydroxylated protein contains a number of unusual amino acids, including 3,4-dihydroxyphenylalanine (DOPA), which is thought to contribute to the crosslinking of the extruded threads and ...

journal_title：Biopolymers

authors： Akemi Ooka A,Garrell RL

更新日期：2000-01-01 00:00:00

abstract：:The interaction of the immunogenic peptide of human hepatitis B virus (HBV) preS(120-145), including B and T epitopes, with phospholipid vesicles has been studied by fluorescence techniques and CD. In addition, interaction of three lipopeptides derived from preS(120-145) containing stearoyl, cholanoyl, and tripalmitoy...

journal_title：Biopolymers

authors： Cajal Y,Rabanal F,Alsina MA,Reig F

更新日期：1996-05-01 00:00:00

abstract：:Small amounts of phenolic compounds are being used as preservatives in pharmaceutical insulin preparations. It has been shown previously that these compounds bind to specific sites on the insulin hexamer and act as allosteric effectors, inducing a transformation of the T6 hexamer to the R6 hexamer, via a T3R3 intermed...

journal_title：Biopolymers

authors： Berchtold H,Hilgenfeld R

更新日期：1999-01-01 00:00:00

abstract：:Host defense peptides are conserved components of innate immune response present among all classes of life. These peptides are potent, broad spectrum antimicrobial agents with potential as novel therapeutic compounds. Also, the ability of host defense peptides to modulate immunity is an emerging therapeutic concept si...

journal_title：Biopolymers

authors： Alba A,López-Abarrategui C,Otero-González AJ

更新日期：2012-01-01 00:00:00

abstract：:Late expression factor 4 (LEF4) is one of the four subunits of Autographa californica nuclear polyhedrosis virus (AcNPV) RNA polymerase. LEF4 was overexpressed in Escherichia coli and recombinant protein was subjected to structural characterization. Chemical induced unfolding of LEF4 was investigated using intrinsic f...

journal_title：Biopolymers

authors： Rasheedi S,Suragani M,Haq SK,Ghosh S,Ehtesham NZ,Hasnain SE

更新日期：2009-07-01 00:00:00

abstract：:Fluorescence binding measurements and molecular modeling were employed to study the interaction of hypericin (Hyp) with human (HSA), rat (RSA), and bovine (BSA) serum albumins. Fluorescence emission data show the solubility of Hyp increasing in the order BSA, HSA, and RSA. Molecular modeling was used to construct the ...

journal_title：Biopolymers

authors： Hritz J,Kascakova S,Ulicny J,Miskovsky P

更新日期：2002-01-01 00:00:00

abstract：:This brief account traces the development of a "competition dialysis" method used to characterize the structural and sequence selectivity of DNA binding compounds. The method was inspired by a simple "differential dialysis" method pioneered by Don Crothers to explore base-selective intercalator binding. Results from c...

journal_title：Biopolymers

authors： Chaires JB

更新日期：2015-09-01 00:00:00

abstract：:Interactions between the diketopiperazine cyclo-alanylglycine and four fluorinated alcohols in water-fluoroalcohol mixtures were examined by (1)H[(19)F] intermolecular nuclear Overhauser effects (NOE) experiments. The alcohols studied were trifluoroethanol, hexafluoroacetone trihydrate, 1,1,1,3,3,3-hexafluoroisopropan...

journal_title：Biopolymers

authors： Strickler MA,Gerig JT

更新日期：2002-08-15 00:00:00

abstract：:Bleomycins A(2) and B(2) are the two active components in the antineoplastic drug Blenoxane. DNA is targeted by this drug in cancer cells and the mode of action of this drug involves DNA binding. Ambiguity exists as to the way in which bleomycin binds to DNA. Raman spectroscopy was used to examine both calf thymus DNA...

journal_title：Biopolymers

authors： Rajani C,Kincaid JR,Petering DH

更新日期：1999-01-01 00:00:00

abstract：:Spiders can produce up to seven different types of silks or glues with different mechanical properties. Of these, flagelliform (Flag) silk is the most elastic, and aciniform (AcSp1) silk is the toughest. To produce a chimeric spider silk (spidroin) FlagR -AcSp1R , we fused one repetitive module of flagelliform silk fr...

journal_title：Biopolymers

authors： Tian LY,Meng Q,Lin Y

更新日期：2020-12-01 00:00:00

abstract：:The pressure-induced structural changes of a de novo designed four-helix bundle protein, (alpha-l-alpha)(2), in aqueous solution have been investigated by FTIR spectroscopy. Changes in the amide I' band intensity show that pressure induces disruption of tertiary interactions and stabilizes the solvated alpha-helical f...

journal_title：Biopolymers

authors： Takekiyo T,Takeda N,Isogai Y,Kato M,Taniguchi Y

更新日期：2007-02-05 00:00:00

abstract：:Peptide-conjugated polysaccharide matrices using bioactive laminin-derived peptides are useful biomaterials for tissue and cell engineering. Here, we demonstrate an easy handling preparation method for peptide-polysaccharide matrices using polyion complex with both alginate and chitosan. First, aldehyde-alginate was s...

journal_title：Biopolymers

authors： Fujimori C,Kumai J,Nakamura K,Gu Y,Katagiri F,Hozumi K,Kikkawa Y,Nomizu M

更新日期：2017-01-01 00:00:00

abstract：:Although the structural analysis of membrane proteins is advancing, an understanding of the basic principles that underlie their folding and assembly remains limited because of the high insolubility intrinsic to these molecules and concomitant challenges in obtaining crystals. Fortunately, from an experimental standpo...

journal_title：Biopolymers

authors： Rath A,Johnson RM,Deber CM

更新日期：2007-01-01 00:00:00

abstract：:MUC1 mucin is a large transmembrane glycoprotein whose extracelluler domain is composed of repeating units of a 20 amino acid sequence. In the cancer associated state, this protein expression becomes upregulated and underglycosylated. Previous studies, which show an enhanced binding of a 5-repeat over a 1-repeat MUC1 ...

journal_title：Biopolymers

authors： Schuman JT,Grinstead JS,Apostolopoulos V,Campbell AP

更新日期：2005-02-05 00:00:00

abstract：:Regulatory, receptor-binding peptides are considered as the agents of choice for diagnostic imaging and therapy of cancers, because their receptors are overexpressed in various human cancer cells. It has been recently indicated that there is a putative role of NPY in breast tumors. The expression of the two best-inves...

journal_title：Biopolymers

authors： Khan IU,Reppich R,Beck-Sickinger AG

更新日期：2007-01-01 00:00:00

abstract：:Serine palmitoyltransferase (SPT) catalyses the first step in the de novo biosynthesis of sphingolipids (SLs). It uses a decarboxylative Claisen-like condensation reaction to couple L-serine with palmitoyl-CoA to generate a long-chain base product, 3-ketodihydrosphingosine. SLs are produced by mammals, plants, yeast, ...

journal_title：Biopolymers

authors： Raman MC,Johnson KA,Clarke DJ,Naismith JH,Campopiano DJ

更新日期：2010-09-01 00:00:00

abstract：:Poly(dimer acid-brassylic acid) [P(DA-BA)] copolymers and poly(dimer acid-pentadecandioic acid) [P(DA-PA)] copolymers were prepared by melt polycondensation of the corresponding mixed anhydride prepolymers. The copolymers were characterized by Fourier transform infrared (FTIR), gel permeation chromatography (GPC), dif...

journal_title：Biopolymers

authors： Guo WX,Huang KX

更新日期：2004-06-15 00:00:00

abstract：:The presence of low pKa N-hydroxylamines is beneficial in peptide chemistry as they reduce some base-mediated side reactions. Here we evaluated the applicability and buffering capacity of Ethyl 2-cyano-2-(hydroxyimino) acetate (Oxyma) in the prevention of aspartimide/piperidide formation and Pro-based overcoupling and...

journal_title：Biopolymers

authors： Subirós-Funosas R,El-Faham A,Albericio F

更新日期：2012-01-01 00:00:00

abstract：:Different hexameric forms of insulin have been crystallized from a variety of conditions. In the presence of 1% phenol, 1.0 M sodium chloride, and at a pH of 8.5, a rhombohedral form is produced with two monomers in the asymmetric unit, space group R3, a = 79.92 A and c = 40.39 A. The structure has been solved and ref...

journal_title：Biopolymers

authors： Smith GD,Dodson GG

更新日期：1992-04-01 00:00:00

abstract：:G protein-coupled receptor kinase 2 (GRK2) plays a central role in the cellular transduction network. In particular, during chronic heart failure GRK2 is upregulated and believed to contribute to disease progression. Thereby, its inhibition offers a potential therapeutic solution to several pathological conditions. In...

journal_title：Biopolymers

authors： Gomez-Monterrey I,Carotenuto A,Cipolletta E,Sala M,Vernieri E,Limatola A,Bertamino A,Musella S,Grieco P,Trimarco B,Novellino E,Iaccarino G,Campiglia P

更新日期：2014-01-01 00:00:00

abstract：:Cytochrome c3 from Desulfovibrio gigas is electrostatically adsorbed on Ag electrodes coated with self-assembled monolayers (SAMs) of 11-mercaptoundecanoic acid. The redox equilibria and electron transfer dynamics of the adsorbed four-heme protein are studied by surface enhanced resonance Raman spectroscopy. Immobiliz...

journal_title：Biopolymers

authors： Simaan AJ,Murgida DH,Hildebrandt P

更新日期：2002-01-01 00:00:00

abstract：:A method is presented for determining the concentrations of peptides and proteins having isodichroic points near 203 nm. The existence of an isodichroic point for a given substance indicates a local two-state (alpha-helix, random coil) population. The mean residue ellipticity at the isodichroic point, [theta lambda i]...

journal_title：Biopolymers

authors： Holtzer ME,Holtzer A

更新日期：1992-12-01 00:00:00

abstract：:A systematic molecular dynamics (MD) simulation has been carried out on collagen-like peptides with different combinations of interruptions in the Gly-X(AA) -Y(AA) repeats. Although experimental studies have been carried out to elucidate the structural consequences of homotrimeric collagen-like peptides, this is the f...

journal_title：Biopolymers

authors： Singam ER,Balamurugan K,Gopalakrishnan R,Subramanian SR,Subramanian V,Ramasami T

更新日期：2012-11-01 00:00:00

abstract：:The folding of membrane proteins was addressed using outer membrane protein porin from the soil bacterium Paracoccus denitrificans (P. den.). IR spectroscopy and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis were used to probe the effect of mutagenesis on the thermal stability of the pr...

journal_title：Biopolymers

authors： Sukumaran S,Zscherp C,Mäntele W

更新日期：2004-05-01 00:00:00

abstract：:Hexameric insulin is an allosteric protein that undergoes transitions between three conformational states (T(6), T(3)R(3), and R(6)). These allosteric states are stabilized by the binding of ligands to the phenolic pockets and by the coordination of anions to the His B10 metal sites. Raman difference (RD) spectroscopy...

journal_title：Biopolymers

authors： Ferrari D,Diers JR,Bocian DF,Kaarsholm NC,Dunn MF

更新日期：2001-01-01 00:00:00

abstract：:Synthetic RNA poly[r(A-T)] has been synthesized and its CD spectral properties compared to those of poly[r(A-U)], poly[d(A-T)], and poly[d(A-U)] in various salt and ethanolic solutions. The CD spectra of poly[r(A-T)] in an aqueous buffer and of poly[d(A-T)] in 70.8% v/v ethanol are very similar, suggesting that they b...

journal_title：Biopolymers

authors： Vorlícková M,Kypr J,Jovin TM,Planck M

更新日期：1990-02-05 00:00:00

abstract：:The de novo design of peptides and proteins has emerged as an approach for investigating protein structure and function. The success relies heavily on the ability to design relatively short peptides that can adopt stable secondary structures. To this end, substitution with α,β-dehydroamino acids, especially α,β-didehy...

journal_title：Biopolymers

authors： Gupta M,Chauhan VS

更新日期：2011-03-01 00:00:00

abstract：:Successful intracellular delivery of various bioactive molecules has been reported using cell-permeating peptides (CPPs) as delivery vectors. To determine the effects of CPPs on the cellular uptake of immunoglobulin Fab fragment, conjugates of a radio-iodinated Fab fragment with CPPs (CPP-(125)I-Fab) derived from HIV-...

journal_title：Biopolymers

authors： Kameyama S,Horie M,Kikuchi T,Omura T,Tadokoro A,Takeuchi T,Nakase I,Sugiura Y,Futaki S

更新日期：2007-01-01 00:00:00

abstract：:The aim of this study was to investigate the effect of dopa and catecholamines on the generation of oxygen active species during oxidation of farmorubicin by "Fenton-like" reagents using chemiluminescent and spectrophotometric techniques. The tested catechols were found to reduce the light emission accompanying oxidat...

journal_title：Biopolymers

authors： Kruk I,Lichszteld K,Aboul-Enein HY

更新日期：2002-01-01 00:00:00

abstract：:Natural cellulose molecules have a strong tendency of being aggregated into larger structures. Thus, the imaging of isolated individual cellulose molecules is hampered for a long time. In this work, we manage to observe, for the first time, the isolated individual natural cellulose chains on a sample surface by means ...

journal_title：Biopolymers

authors： Wan Z,Li L,Cui S

更新日期：2008-12-01 00:00:00