Investigation of the thermal stability of porin from Paracoccus denitrificans by site-directed mutagenesis and Fourier transform infrared spectroscopy.

abstract：:Reactive oxygen species (ROS) have generated a great deal of interest in the clinical field since experimental studies showed the involvement of these species in carcinogenesis. This paper reports the detection of ROS during the decomposition of H2O2 in the presence of samples obtained from tissues of 16 patients with...

journal_title：Biopolymers

authors： Kładny J,Lichszteld K,Kołecki J

更新日期：2004-08-05 00:00:00

abstract：:Regulatory, receptor-binding peptides are considered as the agents of choice for diagnostic imaging and therapy of cancers, because their receptors are overexpressed in various human cancer cells. It has been recently indicated that there is a putative role of NPY in breast tumors. The expression of the two best-inves...

journal_title：Biopolymers

authors： Khan IU,Reppich R,Beck-Sickinger AG

更新日期：2007-01-01 00:00:00

abstract：:Multiconfiguration thermodynamic integration was used to determine the relative binding strength of tacrine and 6-chlorotacrine by Torpedo californica acetylcholinesterase. 6-Chlorotacrine appears to be bound stronger by 0.7+/-0.4 kcal/mol than unsubstituted tacrine when the active site triad residue His-440 is deprot...

journal_title：Biopolymers

authors： Wlodek ST,Antosiewicz J,McCammon JA,Straatsma TP,Gilson MK,Briggs JM,Humblet C,Sussman JL

更新日期：1996-01-01 00:00:00

abstract：:Photon correlation spectroscopy has been used to study the translational (D0) and internal dynamics of monodisperse linear and polydisperse superhelical plasmid-DNAs. Scattering vector dependent correlation functions were measured and analyzed with the inverse Laplace transform CONTIN written by S. Provencher. For sca...

journal_title：Biopolymers

authors： Seils J,Dorfmüller T

更新日期：1991-06-01 00:00:00

abstract：:The de novo design of peptides and proteins has emerged as an approach for investigating protein structure and function. The success relies heavily on the ability to design relatively short peptides that can adopt stable secondary structures. To this end, substitution with α,β-dehydroamino acids, especially α,β-didehy...

journal_title：Biopolymers

authors： Gupta M,Chauhan VS

更新日期：2011-03-01 00:00:00

abstract：:Successful intracellular delivery of various bioactive molecules has been reported using cell-permeating peptides (CPPs) as delivery vectors. To determine the effects of CPPs on the cellular uptake of immunoglobulin Fab fragment, conjugates of a radio-iodinated Fab fragment with CPPs (CPP-(125)I-Fab) derived from HIV-...

journal_title：Biopolymers

authors： Kameyama S,Horie M,Kikuchi T,Omura T,Tadokoro A,Takeuchi T,Nakase I,Sugiura Y,Futaki S

更新日期：2007-01-01 00:00:00

abstract：:The rheological characteristics of gastric and duodenal mucin solutions, the building blocks of the mucus layer that covers the epithelia of the two organs, were investigated using particle tracking microrheology. We used biochemically well characterized purified porcine mucins (MUC5AC and MUC2) as models for human mu...

journal_title：Biopolymers

authors： Georgiades P,Pudney PD,Thornton DJ,Waigh TA

更新日期：2014-04-01 00:00:00

abstract：:Differential scanning calorimetry measurements performed on RNase A in aqueous binary solutions containing different concentrations of urea, tetramethylurea, guanidinium chloride, and guanidinium thiocyanate, and in aqueous ternary solutions, containing the same denaturants plus 1 M trimethylamine N-oxide, TMAO, demon...

journal_title：Biopolymers

authors： Vigorita M,Cozzolino S,Oliva R,Graziano G,Del Vecchio P

更新日期：2018-08-01 00:00:00

abstract：:The sweet protein brazzein isolated from the fruit of the African plant, Pentadiplandra brazzeana Baillon is 2000-500 times sweeter than sucrose and consists of 54 amino acid residues with four intramolecular disulfide bonds. Brazzein was prepared by the fluoren-9-yl-methoxycarbonyl solid-phase method, and was identic...

journal_title：Biopolymers

authors： Izawa H,Ota M,Kohmura M,Ariyoshi Y

更新日期：1996-07-01 00:00:00

abstract：:A highly potent and selective agonist to the tachykinin NK-3 receptor, [pGlu6,N-MePhe8,Aib9] substance P (6-11) (I), was synthesized via the solid phase method. The ED50 of I was 4 nM in the guinea pig ileum in the absence of atropine (NK-1+NK-3 receptors) and this agonist was 5000-fold less potent in the presence of ...

journal_title：Biopolymers

authors： Tallon M,Ron D,Halle D,Amodeo P,Saviano G,Temussi PA,Selinger Z,Naider F,Chorev M

更新日期：1993-06-01 00:00:00

abstract：:Spin-spin and spin-lattice 1H-nmr relaxation times of the sea anemone polypeptide anthopleurin-A were measured at frequencies of 200, 300, 400, and 500 MHz. Relaxation times were fitted iteratively by least squares regression to the isotropic tumbling model, Woessner's model for anisotropic motion, and Lipari and Szab...

journal_title：Biopolymers

authors： Torda AE,Norton RS

更新日期：1989-03-01 00:00:00

abstract：:We have utilized a de novo designed two-stranded α-helical coiled-coil template to display conserved α-helical epitopes from the stem region of hemagglutinin (HA) glycoproteins of influenza A. The immunogens have all the surface-exposed residues of the native α-helix in the native HA protein of interest displayed on t...

journal_title：Biopolymers

authors： Jiang Z,Gera L,Mant CT,Hirsch B,Yan Z,Shortt JA,Pollock DD,Qian Z,Holmes KV,Hodges RS

更新日期：2016-03-01 00:00:00

abstract：:It has recently been reported that synthetic peptides corresponding to the C-terminal sequence of G alpha, can be used to study the molecular mechanisms of interaction between this protein and G protein coupled receptors (Hamm et al., Science, 1988, Vol. 241, pp. 832-835). A conformational analysis on a 11 amino acids...

journal_title：Biopolymers

authors： Albrizio S,D'Ursi A,Fattorusso C,Galoppini C,Greco G,Mazzoni MR,Novellino E,Rovero P

更新日期：2000-09-01 00:00:00

abstract：:The enzymatic activities of native myosin light chain kinases are subject to modification by interaction with Ca2(+)-calmodulin (CaM). The interaction between myosin light chain kinase isolated from turkey gizzard (tgMLCK) and calmodulin isolated from bovine testes (CaMbt) and wheat germ (CaMwg) has been examined by m...

journal_title：Biopolymers

authors： Garone L,Albaugh S,Steiner RF

更新日期：1990-01-01 00:00:00

abstract：:Pig citrate synthase (PCS) can be used as a model enzyme to gain some insight into the structural basis of protein thermostability. The thermal unfolding characteristics of the specific secondary structure elements within PCS were monitored in detail by following changes in its amide I band components. The result of o...

journal_title：Biopolymers

authors： Severcan F,Haris PI

更新日期：2003-08-01 00:00:00

abstract：:The self-cleaving hepatitis delta virus (HDV) ribozyme is essential for the replication of HDV, a liver disease causing pathogen in humans. The catalytically critical nucleotide C75 of the ribozyme is buttressed by a trefoil turn pivoting around an extruded G76. In all available crystal structures, the conformation of...

journal_title：Biopolymers

authors： Sefcikova J,Krasovska MV,Spacková N,Sponer J,Walter NG

更新日期：2007-04-05 00:00:00

abstract：:CD and nmr spectroscopy were used to compare the conformational properties of two related peptides. One of the peptides, Model AB, was designed to adopt a helix-turn-extended strand (alpha beta) tertiary structure in water that might be stabilized by hydrophobic interactions between two leucine residues in the amino-t...

journal_title：Biopolymers

authors： Butcher DJ,Bruch MD,Moe GR

更新日期：1995-08-01 00:00:00

abstract：:A short peptide-based molecule has been found to form a strong hydrogel at phosphate buffer solution of pH 7.46. The hydrogel has been characterized thoroughly using various techniques including field emission scanning electron microscopy (FE-SEM), wide angle powder X-ray diffraction (PXRD), and rheological analysis. ...

journal_title：Biopolymers

authors： Nandi N,Baral A,Basu K,Roy S,Banerjee A

更新日期：2017-01-01 00:00:00

abstract：:Nocistatin, a new heptadecapeptide encoded in the bPNP-3 gene, has a powerful biological activity connected with the mechanisms of pain transmission. It does not bind to the opioid receptors but to another brain receptor with high affinity. In order to substantiate these novel biological data with a structural basis, ...

journal_title：Biopolymers

authors： Crescenzi O,Guerrini R,Picone D,Salvadori S,Tancredi T,Temussi PA

更新日期：2000-03-01 00:00:00

abstract：:The applicability of the FTIR attenuated total reflectance technique for in situ monitoring of plant physiological processes such as leaf senescence and aging has been examined. Difference spectra obtained by subtracting the spectrum of the young plant leaf from that of the older one revealed positive bands at 1650-15...

journal_title：Biopolymers

authors： Ivanova DG,Singh BR

更新日期：2003-01-01 00:00:00

abstract：:Laminins are a multifunctional molecule with numerous active sites that have been identified in short peptide sequences. Mixed peptide-conjugated chitosan membranes using laminin-derived active peptides have been previously demonstrated to be useful as a biomaterial for tissue engineering. In this study, two syndecan-...

journal_title：Biopolymers

authors： Otagiri D,Yamada Y,Hozumi K,Katagiri F,Kikkawa Y,Nomizu M

更新日期：2013-11-01 00:00:00

abstract：:A winter flounder antifreeze polypeptide (HPLC-6) has been studied in vacuo and in aqueous solution using molecular dynamics computer simulation techniques. The helical conformation of this polypeptide was found to be stable both in vacuum and in solution. The major stabilizing interactions were found to be the main-c...

journal_title：Biopolymers

authors： McDonald SM,Brady JW,Clancy P

更新日期：1993-10-01 00:00:00

abstract：:Host defense peptides are conserved components of innate immune response present among all classes of life. These peptides are potent, broad spectrum antimicrobial agents with potential as novel therapeutic compounds. Also, the ability of host defense peptides to modulate immunity is an emerging therapeutic concept si...

journal_title：Biopolymers

authors： Alba A,López-Abarrategui C,Otero-González AJ

更新日期：2012-01-01 00:00:00

abstract：:We describe a method to determine the magnitude of protein-induced DNA bends relative to a set of standard A tract bends using comparative gel electrophoresis. The DNA bend of interest was that induced by the catabolite activator protein (CAP), the transcriptional activator protein of the lac operon. The set of compar...

journal_title：Biopolymers

authors： Zinkel SS,Crothers DM

更新日期：1990-01-01 00:00:00

abstract：:Infrared spectra of the amino acid residues in H2O solution have been obtained in the 1800-1400-cm-1 region. It has been established that amino acid residues of arginine, asparagine, glutamine, aspartic and glutamic acids, lysine, tyrosine, histidine, and phenylalanine have intensive absorption in this spectral region...

journal_title：Biopolymers

authors： Venyaminov SYu,Kalnin NN

更新日期：1990-01-01 00:00:00

abstract：:Spray drying is a way to generate protein solids (powders), which is also true for lyophilization. Sugars are used to protect proteins from conformational changes and chemical degradations arising from drying processes and storage conditions such as the humidity. The influence of trehalose and humidity on the conforma...

journal_title：Biopolymers

authors： French DL,Arakawa T,Li T

更新日期：2004-03-01 00:00:00

abstract：:Nanoparticle based delivery systems can offer opportunities for targeting, controlled release, and enhanced stability of their drug, protein, or gene therapy payload. This study investigated the use of chitosan in combination with the ionic additives sulfobutyl-ether-7-beta-cyclodextrin (SB-CD) or SB-CD/dextran sulfat...

journal_title：Biopolymers

authors： Chen Y,Siddalingappa B,Chan PH,Benson HA

更新日期：2008-01-01 00:00:00

abstract：:Protein flexibility and conformational diversity is well known to be a key characteristic of the function of many proteins. Human blood coagulation proteins have multiple substrates, and various protein-protein interactions are required for the smooth functioning of the coagulation cascade to maintain blood hemostasis...

journal_title：Biopolymers

authors： Singh N,Briggs JM

更新日期：2008-12-01 00:00:00

abstract：:In the first two papers of this series, the tools necessary to evaluate substrate ring deformations were developed, and then the modeling of short amylose fragments (maltotriose and maltopentaose) inside the catalytic site of barley alpha-amylase was performed. In this third paper, this docking has been extended to th...

journal_title：Biopolymers

authors： André G,Buléon A,Haser R,Tran V

更新日期：1999-12-01 00:00:00

abstract：:Flow linear dichroism measurements extended into the vacuum uv region yield inclinations for the base normal from the helix axis of 21 degrees for dA and 40 degrees for dT in the X-form of poly(dAdT).poly(dAdT). These inclination angles are similar to the B form of the synthetic polymer, but the axes around which the ...

journal_title：Biopolymers

authors： Riazance-Lawrence JH,Kang H,Chou PJ,Johnson WC Jr,Vorlícková M

更新日期：1994-11-01 00:00:00