Intramolecular C---H···O hydrogen-bond mediated stabilization of a cis-DPro imide-bond in a stereocontrolled heterochiral model peptide.

abstract：:In order to check our current knowledge on the principles involved in beta-hairpin formation, we have modified the sequence of a 3:5 beta-hairpin forming peptide with two different purposes, first to increase the stability of the formed 3:5 beta-hairpin, and second to convert the 3:5 beta-hairpin into a 2:2 beta-hairp...

journal_title：Biopolymers

authors： Santiveri CM,Pantoja-Uceda D,Rico M,Jiménez MA

更新日期：2005-10-15 00:00:00

abstract：:The pressure-induced structural changes of a de novo designed four-helix bundle protein, (alpha-l-alpha)(2), in aqueous solution have been investigated by FTIR spectroscopy. Changes in the amide I' band intensity show that pressure induces disruption of tertiary interactions and stabilizes the solvated alpha-helical f...

journal_title：Biopolymers

authors： Takekiyo T,Takeda N,Isogai Y,Kato M,Taniguchi Y

更新日期：2007-02-05 00:00:00

abstract：:The chemical synthesis and x-ray crystal structure analysis of a model peptide incorporating a conformationally adaptable unsubstituted beta-Ala residue: Boc-beta-Ala-Acc6-OCH3 (C16H28N2O5, molecular weight = 328.41; 1) has been described. The peptide crystallized in the space group P2(1)2(1)2(1) a = 8.537 (3), b = 8....

journal_title：Biopolymers

authors： Thakur AK,Kishore R

更新日期：2000-05-01 00:00:00

abstract：:In the native structure of hen egg white lysozyme (HEL), the amino acid sequence 87-97 (HEL 87-97) forms an amphiphilic helix, with hydrophilic residues in the sequence directed toward the solvent. A synthetic version of the HEL 87-97 sequence (with the cysteine corresponding to position 94 of HEL replaced by alanine)...

journal_title：Biopolymers

authors： Vuilleumier S,Mutter M

更新日期：1993-03-01 00:00:00

abstract：:With the availability of accurate methods to treat the electrostatic long-range interactions, molecular dynamics simulations have resulted in refined dynamical models of the structure of the hydration shell around RNA motifs. The models reviewed here range from basic Watson-Crick to more specific noncanonical base pai...

journal_title：Biopolymers

authors： Auffinger P,Westhof E

更新日期：2000-01-01 00:00:00

abstract：:TACC3 is a centrosomal adaptor protein that plays important roles during mitotic spindle assembly. It interacts with chTOG/XMAP215, which catalyzes the addition of tubulin dimers during microtubule growth. A 3D coiled-coil model for this interaction is available but the structural details are not well described. To ch...

journal_title：Biopolymers

authors： Partida-Hanon A,Treviño MA,Mompeán M,Jiménez MÁ,Bruix M

更新日期：2017-11-01 00:00:00

abstract：:Recently, the use of hybrid double network (DN) hydrogels has become prominent due to their enhanced mechanical properties, which has opened the door for new applications of these soft materials. Only a few of these gels have demonstrated both injectable and moldable capabilities. In this work, we report the mechanica...

journal_title：Biopolymers

authors： Qiao Z,Mieles M,Ji HF

更新日期：2020-06-01 00:00:00

abstract：:Conformational analysis of the neurohypophyseal hormones oxytocin (OT) and arginine-vasopressin (AVP) has been carried out using two different computational approaches and three force fields, namely by the Electrostatically Driven Monte Carlo (EDMC) method, with the Empirical Conformational Energy Program for Peptides...

journal_title：Biopolymers

authors： Liwo A,Tempczyk A,Ołdziej S,Shenderovich MD,Hruby VJ,Talluri S,Ciarkowski J,Kasprzykowski F,Lankiewicz L,Grzonka Z

更新日期：1996-02-01 00:00:00

abstract：:Raman spectra of series of aqueous solutions of peptides containing two amino acids, glycine-X, alanine-X, and serine-X, where X is an uncharged amino acid, show that the amide III band shifts systematically to lower frequencies as the side chain of the X amino acid becomes larger. The range of this shift is about 20 ...

journal_title：Biopolymers

authors： Weaver JL,Williams RW

更新日期：1990-01-01 00:00:00

abstract：:Spiders can produce up to seven different types of silks or glues with different mechanical properties. Of these, flagelliform (Flag) silk is the most elastic, and aciniform (AcSp1) silk is the toughest. To produce a chimeric spider silk (spidroin) FlagR -AcSp1R , we fused one repetitive module of flagelliform silk fr...

journal_title：Biopolymers

authors： Tian LY,Meng Q,Lin Y

更新日期：2020-12-01 00:00:00

abstract：:Small-angle x-ray scattering was used for studying intermediate species, isolated after heat-induced aggregation of the A variant of bovine beta-lactoglobulin. The intermediates were separated in two fractions, the heated metastable dimer and heated metastable oligomers larger than the dimer. The pair distance distrib...

journal_title：Biopolymers

authors： Carrotta R,Arleth L,Pedersen JS,Bauer R

更新日期：2003-10-01 00:00:00

abstract：:Optical tweezers are ideally suited to perform force microscopy experiments that isolate a single biomolecule, which then provides multiple binding sites for ligands. The captured complex may be subjected to a spectrum of forces, inhibiting or facilitating ligand activity. In the following experiments, we utilize opti...

journal_title：Biopolymers

authors： McCauley MJ,Williams MC

更新日期：2009-04-01 00:00:00

abstract：:IR vibrational CD (VCD) has been observed for the cyclic pentapeptide cyclo-(-Gly-Pro-Gly-D-Ala-Pro-) in solution in CDBr3. The observed VCD spectra do not resemble the VCD features of any of the previously reported peptide secondary structures, such as alpha-helical, "random coil," or sheet structures, and might be d...

journal_title：Biopolymers

authors： Wyssbrod HR,Diem M

更新日期：1992-09-01 00:00:00

abstract：:Through optimization of the linker region and key stabilizing mutations, it has been possible to improve the stability of the circularly permuted (cp) Trp-cage miniprotein. However, even the most stable Trp-cage circular permutants are still less stable than the analogous standard topology (std) Trp-cages. Extending m...

journal_title：Biopolymers

authors： Graham KA,Byrne A,Mason M,Andersen NH

更新日期：2019-12-01 00:00:00

abstract：:The pulsed-field gel electrophoresis (PFG) is a newly developing technique used in the fractionation of large DNA fragments. Advances in PFG demand a better understanding in the corresponding mechanisms of DNA dynamics in the gel network. Detailed experiments are needed to verify and to extend existing theoretical pre...

journal_title：Biopolymers

authors： Chu B,Wang ZL,Xu RL,Lalande M

更新日期：1990-03-01 00:00:00

abstract：:As a model of receptor protein, a series of 3alpha-helix bundle peptides constructed on a template peptide were designed so as to possess a hydrophobic cavity. The size of cavity was modulated by simple replacements of Leu residues to Ala residues in the hydrophobic core. Binding abilities to 8-anilino-1-naphthalenesu...

journal_title：Biopolymers

authors： Obataya I,Sakamoto S,Ueno A,Mihara H

更新日期：2001-08-01 00:00:00

abstract：:Experimental and computational methods of infrared microspectroscopy (IRI-MSP) and infrared spectral mapping (ISM) are presented. These methods are subsequently applied to the analysis of cirrhotic liver tissue. The sensitivity of infrared spectral mapping toward spectral changes caused by disease will be demonstrated...

journal_title：Biopolymers

authors： Diem M,Chiriboga L,Yee H

更新日期：2000-01-01 00:00:00

abstract：:Individual-site isotherms for the binding of bacteriophage lambda repressor to the left and right lambda operators have been determined [D. F. Senear, M. Brenowitz, M. A. Shea, and G. K. Ackers (1986) Biochemistry, Vol. 25, pp. 7344-7354.] using the DNAse protection technique [footprinting; D. J. Galas and A. Schmitz ...

journal_title：Biopolymers

authors： Saroff HA

更新日期：1993-09-01 00:00:00

abstract：:CD experiments are reported for several coiled-coil species in the tyrosine/disulfide (approximately 250-350-nm) region. Intact noncross-linked tropomyosin (approximately 3 degrees C) shows a negative nonsymmetric band maximal at 280 nm. This spectrum is the sum over six tyrosines/chain, and has conformational signifi...

journal_title：Biopolymers

authors： Holtzer ME,Kumar S,Holtzer A,Crimmins DL

更新日期：1989-09-01 00:00:00

abstract：:The development of differential scanning calorimetry has resulted in an increased interest in studies of the unfolding process in proteins with the aim of identifying domains and interactions with ligands or other proteins. Several of these studies were done with actin and showed that the thermal unfolding of F-actin ...

journal_title：Biopolymers

authors： Gicquaud CR,Heppell B

更新日期：2006-11-01 00:00:00

abstract：:Methods to generate fibers from hydrogels, with control over mechanical properties, fiber diameter, and crystallinity, while retaining cytocompatibility and degradability, would expand options for biomaterials. Here, we exploited features of silk fibroin protein for the formation of tunable silk hydrogel fibers. The b...

journal_title：Biopolymers

authors： Bradner SA,Partlow BP,Cebe P,Omenetto FG,Kaplan DL

更新日期：2017-09-01 00:00:00

abstract：:The results are presented for the deconvolution of IR spectra of disaccharides and polysaccharides with alpha and beta configurations of the 1 --> 4 glycosidic linkage (maltose, cellobiose, amylose, and cellulose), as well as of their corresponding monosaccharides (alpha- and beta-D-glucose) in the 1200-920 cm(-1) fre...

journal_title：Biopolymers

authors： Nikonenko NA,Buslov DK,Sushko NI,Zhbankov RG

更新日期：2000-01-01 00:00:00

abstract：:Piscidin 1 (Pis-1) has a high broad-spectrum activity against bacteria, fungi, and viruses but it also has a moderate hemolytic activities. To improve the antibacterial activity and to reduce toxicity, mutants Pis-1AA (G8A/G13A double mutant) and Pis-1PG (G8P mutant) have been designed based on the crystal structure o...

journal_title：Biopolymers

authors： Yuan T,Zhang X,Hu Z,Wang F,Lei M

更新日期：2012-12-01 00:00:00

abstract：:Tissue engineering techniques particularly using electrospun scaffolds have been intensively used in recent years for the development of small diameter vascular grafts. However, the development of a completely successful scaffold that fulfills multiple requirements to guarantee complete vascular regeneration remains c...

journal_title：Biopolymers

authors： Vatankhah E,Prabhakaran MP,Semnani D,Razavi S,Morshed M,Ramakrishna S

更新日期：2014-12-01 00:00:00

abstract：:Peptide-polysaccharide matrices can mimic extracellular matrix structure and function and are useful for tissue and cell engineering. The spacer between the peptide and the polysaccharide is important for both peptide conformation and the interaction between the peptide and receptors. Here, the effect of a spacer on t...

journal_title：Biopolymers

authors： Kumai J,Hozumi K,Yamada Y,Katagiri F,Kikkawa Y,Nomizu M

更新日期：2016-11-04 00:00:00

abstract：:Myosins are a large family of molecular motors that use the common P-loop, Switch 1 and Switch 2 nucleotide binding motifs to recognize ATP, to create a catalytic site than can efficiently hydrolyze ATP and to communicate the state of the nucleotide pocket to other allosteric binding sites on myosin. The energy of ATP...

journal_title：Biopolymers

authors： Geeves MA

更新日期：2016-08-01 00:00:00

abstract：:The comprehensive structural analysis reported herein of eight N-glycopeptides, in three different solvents, is based on quantitative CD experiments, homonuclear nuclear Overhauser effect measurements, and molecular dynamics (MD) calculations. Although several orientations of the two amide planes attached to the carbo...

journal_title：Biopolymers

authors： Perczel A,Kollát E,Hollósi M,Fasman GD

更新日期：1993-04-01 00:00:00

abstract：:The structure of a bacterial cell wall may alter during bacterial reproduction. Moreover, these cell wall variations, on a nanoscale resolution, have not yet fully been elucidated. In this work, Raman spectroscopy and atomic force microscopy (AFM) technique are applied to evaluate the culture time-dependent cell wall ...

journal_title：Biopolymers

authors： McEwen GD,Wu Y,Zhou A

更新日期：2010-02-01 00:00:00

abstract：:Two-dimensional nmr spectra [correlated spectroscopy (COSY), homonuclear Hartmann-Hahn (HOHAHA), nuclear Overhauser effect spectroscopy (NOESY)] have been observed for cross-linked lysozyme, a chemically modified lysozyme derivative with an extra ester cross-link between residues E35 and W108. Eight shifted cross-peak...

journal_title：Biopolymers

authors： Noda Y,Fukuda Y,Segawa S

更新日期：1997-02-01 00:00:00

abstract：:A new series of oxy-peptide nucleic acids (pyrrolidine-based oxy-peptide nucleic acids = POPNAs) of four different stereoisomeric forms (cis-L, cis-D, trans-L, trans-D) have been synthesized. To find a favorable stereoisomer of POPNA for hybridization with DNA or RNA, thermodynamic parameters and conformations of the ...

journal_title：Biopolymers

authors： Kitamatsu M,Shigeyasu M,Saitoh M,Sisido M

更新日期：2006-01-01 00:00:00