Abstract:
:TACC3 is a centrosomal adaptor protein that plays important roles during mitotic spindle assembly. It interacts with chTOG/XMAP215, which catalyzes the addition of tubulin dimers during microtubule growth. A 3D coiled-coil model for this interaction is available but the structural details are not well described. To characterize this interaction at atomic resolution, we have designed a simplified version of the system based on small peptides. Four different peptides have been studied by circular dichroism and nuclear magnetic resonance both singly and in all possible combinations; namely, five peptide pairs and two trios. In cosolvents, all single peptides tend to adopt helical conformations resembling those of the full-length protein. However, neither the single peptides nor pairs of peptides form coiled coils. We show that the simultaneous presence of all preformed helices is a prerequisite for binding. The simplest 3D model for the interaction, based on the NMR results, is proposed. Interestingly, the peptide's structure remains unaffected by mutations at essential positions for TACC3 activity. This suggests that the lack of interaction of this TACC3 mutant with XMAP does not correlate with changes in the protein structure and that specific interactions are likely responsible for the interaction and stability of the complex.
journal_name
Biopolymersjournal_title
Biopolymersauthors
Partida-Hanon A,Treviño MA,Mompeán M,Jiménez MÁ,Bruix Mdoi
10.1002/bip.23039subject
Has Abstractpub_date
2017-11-01 00:00:00issue
11eissn
0006-3525issn
1097-0282journal_volume
107pub_type
杂志文章相关文献
BIOPOLYMERS文献大全abstract::The thermodynamics of the stacking to unstacking transitions of 24 single-stranded DNA sequences (ssDNA), 10-12 bases in length, in sodium phosphate buffer were determined from 10 to 95 degrees C, using differential scanning calorimetry (DSC). An additional 22 ssDNA sequences did not exhibit an S<=>U transition in thi...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21044
更新日期:2008-11-01 00:00:00
abstract::The physicochemical characteristics of the green fluorescent protein (GFP), including the thermodynamic properties (entropy, enthalpy, Gibbs' free energy, heat capacity), normal mode vibrations, and atomic fluctuations, were investigated. The Gaussian 03 computational chemistry program was employed for normal mode ana...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20264
更新日期:2005-06-15 00:00:00
abstract::The enzymatic activities of native myosin light chain kinases are subject to modification by interaction with Ca2(+)-calmodulin (CaM). The interaction between myosin light chain kinase isolated from turkey gizzard (tgMLCK) and calmodulin isolated from bovine testes (CaMbt) and wheat germ (CaMwg) has been examined by m...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360301113
更新日期:1990-01-01 00:00:00
abstract::Alpha-synuclein (alpha-Syn) has been identified as a component of intracellular fibrillar deposits in Parkinson's disease. Though the real pathogenesis is still unknown, many investigations have revealed that conformational alteration and fibril formation of alpha-Syn protein have an important role in causing the dise...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20550
更新日期:2006-10-15 00:00:00
abstract::The conformational behavior of the synthetic peptide, Boc-Gly-Val-Gly-Gly-Leu-OMe, containing the X-Gly-Gly and Gly-Gly-X (X = Val or Leu) repeating sequences and constituting a fragment of elastin was investigated by molecular mechanics and molecular dynamics (MD) simulation. The results suggest that, irrespective of...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360320206
更新日期:1992-02-01 00:00:00
abstract::Surfactant protein C (SP-C) is an important constituent of lung surfactant (LS) and, along with SP-B, is included in exogenous surfactant replacement therapies for treating respiratory distress syndrome (RDS). SP-C's biophysical activity depends upon the presence of a rigid C-terminal helix, of which the secondary str...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.23277
更新日期:2019-06-01 00:00:00
abstract::A series of novel nonpeptide inhibitors of the pp60(c-Src) (Src) SH2 domain is described that exploit multifunctional group replacement of the phenylphosphate moiety of phosphotyrosine (pTyr). Relative to an x-ray structure of citrate complexed to the pTyr binding site of the Src SH2 domain, these nonpeptide ligands i...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10600
更新日期:2003-01-01 00:00:00
abstract::In this study a novel general approach is presented that allows for a straightforward design of receptor binding assays. This principle of a receptor binding assay is applied to the estrogen receptor, which is important in the management of breast cancer and for the estimation of the estrogenic potency of chemicals in...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10402
更新日期:2003-01-01 00:00:00
abstract::In recent years, a revolution has occurred in the basic sciences, which exploits novel single molecule detection and manipulation tools to track and analyze biopolymers in unprecedented detail. A recent Gordon Research Conference style meeting, hosted by the University of Michigan, highlighted current status and futur...
journal_title:Biopolymers
pub_type:
doi:10.1002/bip.20621
更新日期:2007-02-05 00:00:00
abstract::Combinatorial chemistry has recently burst on the scene as a valuable tool for the discovery of new drug candidates. The ability to synthesize hundreds of compounds for screening is a useful complement to rational drug design. There are many similarities between the design of new therapeutic agents and the development...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20395
更新日期:2006-01-01 00:00:00
abstract::Ribonuclease A is the archetype of a functionally diverse superfamily of vertebrate-specific ribonucleases. Inhibitors of its action have potential use in the elucidation of the in vivo roles of these enzymes and in the treatment of pathologies associated therewith. Derivatives of adenosine 5'-pyrophosphate are the mo...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21158
更新日期:2009-12-01 00:00:00
abstract::Cathelicidins are a numerous group of mammalian proteins that carry diverse antimicrobial peptides at the C-terminus of a highly conserved preproregion. These peptides, which become active when released from the proregion, display a remarkable variety of sizes, sequences, and structures, and in fact comprise represent...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/1097-0282(2000)55:1<31::AID-BIP40>3.0.CO;2
更新日期:2000-01-01 00:00:00
abstract::Peptide-bond modification via N-hydroxylation has been explored as a strategy for metal coordination to induce conformational rigidity and orient side chains for specific molecular recognition. N-Hydroxyamides were prepared by reacting N-benzyloxyamino acid esters or amides with Fmoc-AA-Cl/AgCN (Fmoc: 9-fluorenylmetho...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10471
更新日期:2003-01-01 00:00:00
abstract::An examination of x-ray structures of single-cluster [4Fe-4S] proteins in the Protein Data Bank has revealed that all redox proteins and the glutamine 5-phosphoribosyl-l-pyrophosphate amidotransferase from Bacillus subtilis have a topological configuration arbitrarily designated as D, whereas the DNA repair enzyme end...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0282(19971005)42:4<411::AID-BIP
更新日期:1997-10-05 00:00:00
abstract::The HPLC retention behavior of three complete single methionine and methionine sulfoxide replacement sets of two 18-mer model peptides and neuropeptide Y (NPY) were investigated. All peptides were prepared by multiple solid-phase peptide synthesis. Plotting the retention time differences between methionine and methion...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0282(199608)39:2%3C207::AID-BIP
更新日期:1996-08-01 00:00:00
abstract::A new representation of protein sequence is devoted in this paper, in which each protein can be represented by a 20-dimensional (20D) vector of unit length. Inspired by the principle of superposition of state in quantum mechanics, the squares of the 20 components of the vector correspond to the amino acid composition....
journal_title:Biopolymers
pub_type:
doi:10.1002/1097-0282(20010415)58:5<491::AID-BIP1024>3
更新日期:2001-04-15 00:00:00
abstract::Recent results from molecular dynamics (MD) simulations on hydration of DNA with respect to conformation are reviewed and compared with experimental data. MD simulations of explicit solvent around DNA can now give a detailed model of DNA that not only matches well with the experimental data but provides additional ins...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0282(1998)48:4<199::AID-BIP2>3.
更新日期:1998-01-01 00:00:00
abstract::A new family of small plant peptides was recently described and found to be widespread throughout the Millereae and Heliantheae tribes of the sunflower family Asteraceae. These peptides originate from the post-translational processing of unusual seed-storage albumin genes, and have been termed PawS-derived peptides (P...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22901
更新日期:2016-11-01 00:00:00
abstract::Inhibitor design against viral targets must take into account the peculiar characteristics of viral biology-in particular, the plasticity of their replicative machinery. This includes maturational cleavage of the polyprotein, which is mediated by virally encoded proteases. Designing against a movable target is particu...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.10230
更新日期:2002-01-01 00:00:00
abstract::Peptoids, oligomers of N-substituted glycine, have been valuable targets for study and diverse application as peptidomimetics and as nanomaterials. Their conformational heterogeneity has made the study of peptoid structures using high-resolution analyses challenging, limiting our understanding of the physiochemical fe...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21605
更新日期:2011-01-01 00:00:00
abstract::The folding of membrane proteins was addressed using outer membrane protein porin from the soil bacterium Paracoccus denitrificans (P. den.). IR spectroscopy and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis were used to probe the effect of mutagenesis on the thermal stability of the pr...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20049
更新日期:2004-05-01 00:00:00
abstract::Using a variety of spectroscopic techniques, a number of molecular functionalities have been studied in relation to the adsorption process of proteins to air-water interfaces. While ellipsometry and drop tensiometry are used to derive information on adsorbed amount and exerted surface pressure, external reflection cir...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20036
更新日期:2004-05-01 00:00:00
abstract::The indirect solvent-induced effect on the free energy of binding of biopolymers is examined within the framework of classical statistical mechanics. We focus specifically on the role of the solute-solvent hydrogen bonding. In particular, we have estimated the first order solvent effect on the indirect interaction bet...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360290604
更新日期:1990-05-01 00:00:00
abstract::RNA and DNA molecules experience motions on a wide range of time scales, ranging from rapid localized motions to much slower collective motions of entire helical domains. The many functions of RNA in biology very often require this molecule to change its conformation in response to biological signals in the form of sm...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.20650
更新日期:2007-08-05 00:00:00
abstract::We prepared the water soluble model peptide, (E)(8) GGLGGQGAG(A)(6) GGAGQGGYGG, to throw light on the local structure of spidroin 1 (MaSpl) protein in spider dragline silk of Nephila clavipes before and after spinning. Solution (13) C NMR showed that the conformation of the peptide in aqueous solution was essentially ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21718
更新日期:2012-06-01 00:00:00
abstract::Myosins are a large family of molecular motors that use the common P-loop, Switch 1 and Switch 2 nucleotide binding motifs to recognize ATP, to create a catalytic site than can efficiently hydrolyze ATP and to communicate the state of the nucleotide pocket to other allosteric binding sites on myosin. The energy of ATP...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.22853
更新日期:2016-08-01 00:00:00
abstract::The enhanced nucleophilicity and redox sensitivity of the thiol group renders cysteine residues susceptible to numerous electrophilic and oxidative post-translational modifications to form disulfides, sulfenic acids, nitrosothiols, and lipid-modified species. Outside of these well-characterized modifications of cystei...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.22252
更新日期:2014-02-01 00:00:00
abstract::The solid state conformational analysis of [Tyr4] cyclolinopeptide A has been carried out by x-ray diffraction studies. The crystal structure of the monoclinic form, grown from a dioxane-water mixture [alpha = 9.849 (5) A, b = 20.752 (4) A, c = 16.728 (5) A, beta = 98.83 (3) degrees, space group P21, Z = 2], shows the...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360360408
更新日期:1995-10-01 00:00:00
abstract::Recently, we isolated and purified a neutral polysaccharide (PGN) from edible fungus Pleurotus geestanus. Its structure was characterized by a range of physical-chemical methods, including high performance anion exchange chromatography, uronic acid, and protein analyses, size exclusion chromatography with ultraviolet,...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21303
更新日期:2010-02-01 00:00:00
abstract::Tissue engineering techniques particularly using electrospun scaffolds have been intensively used in recent years for the development of small diameter vascular grafts. However, the development of a completely successful scaffold that fulfills multiple requirements to guarantee complete vascular regeneration remains c...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22524
更新日期:2014-12-01 00:00:00