Abstract:
:A series of novel nonpeptide inhibitors of the pp60(c-Src) (Src) SH2 domain is described that exploit multifunctional group replacement of the phenylphosphate moiety of phosphotyrosine (pTyr). Relative to an x-ray structure of citrate complexed to the pTyr binding site of the Src SH2 domain, these nonpeptide ligands illustrate the systematic replacement of the phosphate group by multiple nonhydrolyzable, mono- or dianionic functionalities. Specifically, several phenylalanine (Phe) analogs incorporating key 4' and 3' substituents were synthesized and incorporated into a bicyclic benzamide template previously reported (W. C. Shakespeare et al., Proceedings of the National Academy of Science USA, 2000, Vol. 97, pp. 9373-9378). These pTyr mimetics included 4',3'-diphosphono-Phe (Dpp), 4',3'-dicarboxymethyloxy-Phe (Dcp), and 4'-phosphono-3'-carboxymethyloxy-Phe (Cpp). Noteworthy were nonpeptide inhibitors 8-11 that were 5- to 10-fold more potent than the cognate tetrapeptide ligand Ac-pTyr-Glu-Glu-Ile-NH(2) in binding to the Src SH2 domain.
journal_name
Biopolymersjournal_title
Biopolymersauthors
Sundaramoorthi R,Kawahata N,Yang MG,Shakespeare WC,Metcalf CA 3rd,Wang Y,Merry T,Eyermann CJ,Bohacek RS,Narula S,Dalgarno DC,Sawyer TKdoi
10.1002/bip.10600subject
Has Abstractpub_date
2003-01-01 00:00:00pages
717-29issue
6eissn
0006-3525issn
1097-0282journal_volume
71pub_type
杂志文章相关文献
BIOPOLYMERS文献大全abstract::Although several models have been proposed for the interaction of collagen with gelatinase-A (matrix metalloproteinases-2 (MMP-2)), the extensive role of each domain of gelatinase A in hydrolyzing the collagens with and without interruptions is still elusive. Molecular docking, molecular dynamics (MD) simulation, norm...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22457
更新日期:2014-07-01 00:00:00
abstract::In a seminal paper, Pakula and Sauer (Nature, 1990, 344, 363-364) demonstrated that the increase in side-chain hydrophobicity has a reverse relationship with protein stability. We have addressed this problem with several examples of mutants that span at different locations in protein structure based on secondary struc...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21187
更新日期:2009-07-01 00:00:00
abstract::The characterization of elastic properties of biopolymers is crucial to understand many molecular reactions determined by conformational bending fluctuations of the polymer. Direct measurement of such elastic properties using single-molecule methods is usually hindered by the intrinsic tendency of such biopolymers to ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22533
更新日期:2014-12-01 00:00:00
abstract::The formation mechanism of an alanine-based peptide has been studied by all-atom molecular dynamics simulations with a recently developed all-atom point-charge force field and the Generalize Born continuum solvent model at an effective salt concentration of 0.2M. Thirty-two simulations were conducted. Each simulation ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10216
更新日期:2003-01-01 00:00:00
abstract::The results are presented for the deconvolution of IR spectra of disaccharides and polysaccharides with alpha and beta configurations of the 1 --> 4 glycosidic linkage (maltose, cellobiose, amylose, and cellulose), as well as of their corresponding monosaccharides (alpha- and beta-D-glucose) in the 1200-920 cm(-1) fre...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/1097-0282(2000)57:4<257::AID-BIP7>3.0.CO;2
更新日期:2000-01-01 00:00:00
abstract::Cyclotides are multifunctional plant cyclic peptides containing 28-37 amino acid residues and a pattern of three disulfide bridges, forming a motif known as the cyclic cystine knot. Due to their high biotechnological potential, the sequencing and characterization of cyclotide genes are crucial not only for cloning and...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22938
更新日期:2016-11-01 00:00:00
abstract::Synthesis and CD and (13)C(alpha)-NMR studies in a near-neutral saline buffer are reported for a 65-residue peptide ((190)Tm(254)) comprising residues 190-254 of the alpha-tropomyosin chain. CD on a version disulfide cross-linked via the N-terminal cysteine side chains indicates that this dimer is highly helical and m...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/1097-0282(20011005)59:4<257::AID-BIP1022>3
更新日期:2001-10-05 00:00:00
abstract::The x-ray conformations of 5-, 7-, 9-, and 12-residue loops in bovine pancreatic trypsin inhibitor (BPTI) were predicted by the use of multiple independent Monte Carlo simulating annealing (MCSA) runs starting from random conformations. Four buried water molecules interacted with a 12-residue loop that started at resi...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/1097-0282(20010405)58:4<359::AID-BIP1013>3
更新日期:2001-04-05 00:00:00
abstract::The degradation of elastin, the insoluble biopolymer of tropoelastin, can lead to the production of small peptides. These elastin-derived peptides (EDPs) are playing a key role in cellular behavior within the extracellular matrix, showing a great variety of biological effects such as chemotaxis, stimulation of cell pr...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20276
更新日期:2005-07-01 00:00:00
abstract::The various motifs of RNA molecules are closely related to their structural and functional properties. To better understand the nature and distributions of such structural motifs (i.e., paired and unpaired bases in stems, junctions, hairpin loops, bulges, and internal loops) and uncover characteristic features, we ana...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10525
更新日期:2004-02-15 00:00:00
abstract::A series of cyclic retro-inverso dipeptides--2-[(4-hydroxy)benzyl]-5-benzyl-4,6(1H,2H,3H,5H)-pyrimidinedi one (c[mPhe-gTyr]), 2-benzyl-5-[(4-hydroxy)benzyl]-4,6(1H,2H,3H,5H)-pyrimidinedione (c[mTyr-gPhe]), and 2-benzyl-5-amino-5-[(4-hydroxy)benzyl]-4,6(1H,2H,3H,5H)-pyrimidinedione (c[(alpha-amino)mTyr-gPhe])--were syn...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360311307
更新日期:1991-11-01 00:00:00
abstract::Porins from outer membrane of Gram-negative bacteria have a highly stable structure. Our previous studies on porin from Paracoccus denitrificans showed that the outer membrane protein porin is extremely stable toward heat, pH, and chemical denaturants. The major question we have addressed in this paper is whether the ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20422
更新日期:2006-07-01 00:00:00
abstract::The spectroscopic properties of syringomycin E, an antibiotic lipodepsinonapeptide associated with pathological states in plants, have been investigated by uv absorbance and CD spectroscopies, and by the synthesis of relevant model compounds. Initial studies [E. Vaillo, A. Ballio, P. L. Luisi, and R. M. Thomas (1990) ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360321006
更新日期:1992-10-01 00:00:00
abstract::While our understanding of the folding and structure of water-soluble proteins has progressed to the point where they can be artificially designed and produced from first principles, there has been only limited work toward the de novo design of membrane proteins. Such studies have been hindered in large part due to th...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22149
更新日期:2012-01-01 00:00:00
abstract::The discovery of the prototype delta opioid antagonists TIPP (H-Tyr-Tic-Phe-Phe-OH) and TIP (H-Tyr-Tic-Phe-OH) in 1992 was followed by extensive structure-activity relationship studies, leading to the development of analogues that are of interest as pharmacological tools or as potential therapeutic agents. Stable TIPP...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0282(1999)51:6<411::AID-BIP4>3.
更新日期:1999-01-01 00:00:00
abstract::DNA sequences d-TGAGGAAAGAAGGT (a 14-mer) and d-CTCCTTTCTTCC (a 12-mer) are complementary in parallel orientation forming either Donohue (reverse Watson-Crick) base pairing at neutral pH or Hoogsteen base pairing at slightly acidic pH. The structure of the complex formed by dissolving the two strands in equimolar rati...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0282(199706)41:7<773::AID-BIP6>
更新日期:1997-06-01 00:00:00
abstract::Fourteen tryptic peptides and nine intermediates were identified as products of trypsin digestion of reduced and S-3-(trimethylated amino) propylated lysozyme. Kinetics of the appearance and disappearance of these products were observed by monitoring the peak areas on the chromatogram. In spite of the complicated reac...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360340208
更新日期:1994-02-01 00:00:00
abstract::A large number of potent and selective therapeutic agents, useful for the treatment of several diseases, have been isolated from natural sources. For example, the most active thrombin inhibitors are those secreted by the salivary glands of leeches. One peculiar feature of these agents is the lack of any significant in...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0282(1999)51:1<19::AID-BIP4>3.0
更新日期:1999-01-01 00:00:00
abstract::Vibrational spectroscopy coupled with thermogravimetry (TG) and differential scanning calorimetry (DSC) was used to characterize racemic propanoic acid, 2-methyl-5,6,7,8-tetrahydro-6-(methylamino)-1,2-naphthalenediyl ester hydrochloride (CHF-1035), which is a new DA2 dopaminergic receptor/alpha2 agonist and beta block...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10089
更新日期:2002-01-01 00:00:00
abstract::We report on an unrestrained molecular dynamics simulation of the flavin mononucleotide (FMN)-RNA aptamer. The simulated average structure maintains both cross-strand and intermolecular FMN-RNA nuclear Overhauser effects from the nmr experiments and has all qualitative features of the nmr structure including the G10-U...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0282(199909)50:3<287::AID-BIP5>
更新日期:1999-09-01 00:00:00
abstract::Shorter analogues of a continuous epitope of hepatitis A virus, VP3(110-121) peptide, failed to react with convalescent sera, indicating the importance of the entire peptide in the epitope structure. To better understand the influence of the structural properties of this 12-mer peptide epitope on its biological activi...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/1097-0282(200012)54:7<477::AID-BIP10>3.0.C
更新日期:2000-12-01 00:00:00
abstract::Understanding the high temperature behavior of collagen and collagenous tissue is important for surgical procedures and biomaterials processing for the food, pharmaceutical, and cosmetics industries. One primary event for proteins is thermal denaturation that involves unfolding the polypeptide chains while maintaining...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.23017
更新日期:2017-06-01 00:00:00
abstract::Flow linear dichroism measurements extended into the vacuum uv region yield inclinations for the base normal from the helix axis of 21 degrees for dA and 40 degrees for dT in the X-form of poly(dAdT).poly(dAdT). These inclination angles are similar to the B form of the synthetic polymer, but the axes around which the ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360341104
更新日期:1994-11-01 00:00:00
abstract::Computer simulations can provide in principle quantitative correlation between the structures of DNA polymerases and the replication fidelity. This paper describes our progress in this direction. Using several theoretical approaches, including the free energy perturbation (FEP), linear response approximation (LRA), an...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10244
更新日期:2003-03-01 00:00:00
abstract::A theoretical method is proposed to identify structural domains in proteins of known structures. It is based on the distribution of the local axes of the polypeptide chain. In particular, a statistical analysis is applied to the contributions of the local axes to the absolute writhing number, a topological property of...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/1097-0282(200102)58:2<218::AID-BIP100>3.0.
更新日期:2001-02-01 00:00:00
abstract::Cellulose acetate (DS = 2.45) was extensively investigated by Circular Dichroism (CD) in acetonitrile and dioxane. We found great differences between the CD spectra of a 1 wt % acetonitrile solution and the corresponding dioxane solution of cellulose acetate (CA) indicating that the macromolecules exist in those solut...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0282(199908)50:2<163::AID-BIP5>
更新日期:1999-08-01 00:00:00
abstract::Cyclotides are disulfide-rich miniproteins with a circular backbone and a knotted arrangement ofdisulfide bonds. Because these plant-derived peptides are resistant to degradation and exhibit a diverse range of bioactivity they have become important agronomic and industrial objectives. They belong to a group of compoun...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.21466
更新日期:2010-01-01 00:00:00
abstract::Functional RNAs such as ribosomal RNA and structured domains of mRNA are targets for small molecule ligands that can act as modulators of the RNA biological activity. Natural ligands for RNA display a bewildering structural and chemical complexity that has yet to be matched by synthetic RNA binders. Comparison of natu...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.10410
更新日期:2003-09-01 00:00:00
abstract::Human saliva contains hundreds of small proline-rich peptides originated by the proteolytic cleavage of the salivary basic Proline-Rich Proteins. Nevertheless only for few of them a specific biological activity has been assigned to date. Among them, the 1932 Da peptide (p1932) has been patented as an anti-HIV agent. I...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22889
更新日期:2016-09-01 00:00:00
abstract::Because of its involvement in HIV entry, the chemokine receptor CXCR4 is an attractive target for antiretroviral drugs. Despite the large number of CXCR4 inhibitors studied, the 3D pharmacophore for binding to CXCR4 remains elusive, mainly as a result of conformational flexibility inherent in the identified ligands. I...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20508
更新日期:2006-01-01 00:00:00