CD and (13)C(alpha)-NMR studies of folding equilibria in a two-stranded coiled coil formed by residues 190-254 of alpha-tropomyosin.

abstract：:H-Pro-Pro-Asp-NH2 is a highly active and selective catalyst for asymmetric aldol reactions. Here, the versatility of H-Pro-Pro-Asp-NH2 has been further improved by immobilization on a solid support and functionalization with a short polyethylene glycol linker at the C-terminus. The development, synthesis, and the cata...

journal_title：Biopolymers

authors： Revell JD,Gantenbein D,Krattiger P,Wennemers H

更新日期：2006-01-01 00:00:00

abstract：:The aminoacyl-tRNA synthetases are an ancient group of enzymes that catalyze the covalent attachment of an amino acid to its cognate transfer RNA. The question of specificity, that is, how each synthetase selects the correct individual or isoacceptor set of tRNAs for each amino acid, has been referred to as the second...

journal_title：Biopolymers

authors： Beuning PJ,Musier-Forsyth K

更新日期：1999-01-01 00:00:00

abstract：:The mechanisms of bridge-mediated electron transfer (ET) reactions vary from coherent deep tunneling to thermally activated hopping. This short review focuses on some developments in the study of protein ET mechanisms at the molecular and at the cellular levels. It explains experimental and theoretical work on the inf...

journal_title：Biopolymers

authors： Skourtis SS

更新日期：2013-01-01 00:00:00

abstract：:Rules relating the stereochemistry of N-Dnp (Dnp: 2,4-dinitrophenyl) derivatives of alpha-amino acids and peptides and the sign of the Cotton effects at the longest wavelength band (ca. 400 nm) are surveyed. Some new data and insights concerning the CD spectra of Dnp-alpha-amino acids are included: i.e., the spectra o...

journal_title：Biopolymers

authors： Kawai M,Nagai U,Inai Y,Yamamura H,Akasaka R,Takagi S,Miwa Y,Taga T

更新日期：2005-01-01 00:00:00

abstract：:A water-insoluble polysaccharide (TM8) was isolated from sclerotium of Pleurotus tuber-regium by extraction with 0.5M NaOH aqueous solutions at 120 degrees C. Its chemical structure was confirmed by infrared, high performance liquid chromatography, gas chromatography, and (13)C NMR in dimethylsulfoxide (DMSO) to be co...

journal_title：Biopolymers

authors： Zhang L,Zhang M,Dong J,Guo J,Song Y,Cheung PC

更新日期：2001-11-01 00:00:00

abstract：:The conformation of thymosin beta 9 in solution of 40% (v/v) 1,1,1,3,3,3-hexafluoro-2-propanol-d2 in water has been investigated by two-dimensional 1H-nmr spectroscopy. Under this condition thymosin beta 9 adopts an ordered structure. The determination of the conformation of the peptide was based on a set of 304 appro...

journal_title：Biopolymers

authors： Stoll R,Voelter W,Holak TA

更新日期：1997-05-01 00:00:00

abstract：:Late expression factor 4 (LEF4) is one of the four subunits of Autographa californica nuclear polyhedrosis virus (AcNPV) RNA polymerase. LEF4 was overexpressed in Escherichia coli and recombinant protein was subjected to structural characterization. Chemical induced unfolding of LEF4 was investigated using intrinsic f...

journal_title：Biopolymers

authors： Rasheedi S,Suragani M,Haq SK,Ghosh S,Ehtesham NZ,Hasnain SE

更新日期：2009-07-01 00:00:00

abstract：:The presence of low pKa N-hydroxylamines is beneficial in peptide chemistry as they reduce some base-mediated side reactions. Here we evaluated the applicability and buffering capacity of Ethyl 2-cyano-2-(hydroxyimino) acetate (Oxyma) in the prevention of aspartimide/piperidide formation and Pro-based overcoupling and...

journal_title：Biopolymers

authors： Subirós-Funosas R,El-Faham A,Albericio F

更新日期：2012-01-01 00:00:00

abstract：:Fluorescence binding measurements and molecular modeling were employed to study the interaction of hypericin (Hyp) with human (HSA), rat (RSA), and bovine (BSA) serum albumins. Fluorescence emission data show the solubility of Hyp increasing in the order BSA, HSA, and RSA. Molecular modeling was used to construct the ...

journal_title：Biopolymers

authors： Hritz J,Kascakova S,Ulicny J,Miskovsky P

更新日期：2002-01-01 00:00:00

abstract：:Partially acetylated, high molecular weight glucuronans were produced by a Sinorhizobium meliloti mutant strain. Two native glucuronan samples with various degrees of acetylation were sonicated to obtain lower molecular weight samples and with low viscosity suitable for chemical modification and (13)C NMR experiments....

journal_title：Biopolymers

authors： Pau-Roblot C,Petit E,Sarazin C,Courtois J,Courtois B,Barbotin JN,Séguin JP

更新日期：2002-06-01 00:00:00

abstract：:The beta-subunit of the human chorionic gonadotropin (hCG) hormone, which is believed to be related to certain types of cancer, contains three hairpin-like fragments. To investigate the role of beta-hairpin formation in the early stages of the hCGbeta folding, a 28-residue peptide with the sequence RDVRFESIRLPGSPRGVNP...

journal_title：Biopolymers

authors： Silva RA,Sherman SA,Keiderling TA

更新日期：1999-10-05 00:00:00

abstract：:The abnormal aggregation of amyloid proteins is reported to play a critical role in the etiology of neurodegenerative disorders. Studies have shown that excessive ferric irons are associated with the misfolding of amyloid proteins, and that (-)-epigallocatechin gallate (EGCG) is a good metallic ion chelator with inhib...

journal_title：Biopolymers

authors： Xu L,Tu S,Chen C,Zhao J,Zhang Y,Zhou P

更新日期：2016-02-01 00:00:00

abstract：:The development of differential scanning calorimetry has resulted in an increased interest in studies of the unfolding process in proteins with the aim of identifying domains and interactions with ligands or other proteins. Several of these studies were done with actin and showed that the thermal unfolding of F-actin ...

journal_title：Biopolymers

authors： Gicquaud CR,Heppell B

更新日期：2006-11-01 00:00:00

abstract：:Three new polyoxaalkyl esters of lasalocid are synthesized. Their ability to form complexes with Li(+) and Na(+) cations is studied using multinuclear NMR methods, FTIR spectroscopy in the middle and far IR regions, and mass spectrometry. It is found that lasalocid esters form only 1:1 complexes with the metal cations...

journal_title：Biopolymers

authors： Pankiewicz R,Schroeder G,Gierczyk B,Brzezinski B,Bartl F

更新日期：2002-10-15 00:00:00

abstract：:Substance P, one of the mammalian tachykinins, is known to interact strongly with lipid bilayers and this interaction may play a role in the receptor-peptide recognition process. The conformation of substance P bound to vesicles consisting of perdeuterated phosphatidylcholine has been investigated by means of two-dime...

journal_title：Biopolymers

authors： Augé S,Bersch B,Tropis M,Milon A

更新日期：2000-10-15 00:00:00

abstract：:Insect neuropeptides of the insect kinin class share a common C-terminal pentapeptide sequence F(1)X(1)(2)X(2)(3)W(4)G(5)-NH(2) (X(2)(3) = P, S, A) and regulate such critical physiological processes as water balance and digestive enzyme release. Analogs of the insect kinin class, in which the critical residues of F(1)...

journal_title：Biopolymers

authors： Zubrzak P,Williams H,Coast GM,Isaac RE,Reyes-Rangel G,Juaristi E,Zabrocki J,Nachman RJ

更新日期：2007-01-01 00:00:00

abstract：:Sequence-specific optical signals are used to establish long-range sequence order and identification for fragments hundreds of kilo bases in length. ...

journal_title：Biopolymers

authors： Crothers DM

更新日期：2013-12-01 00:00:00

abstract：:Structural formation of segments plays pivotal roles in protein folding and stability, but how the segment influences the structural ensemble remains elusive. We engineered two hybrid proteins by replacing the central helical segment of immunoglobulin G binding domain of streptococcal protein G with an alpha-helix or ...

journal_title：Biopolymers

authors： Du HN,Zhang TY,Chang YG,Lin DH,Hu HY

更新日期：2005-09-01 00:00:00

abstract：:Recent advances in the application of solid state nmr spectroscopy to uniformly aligned biopolymers have opened a window through which to view the detailed structure of biological macromolecules that are unable to be seen with standard techniques for structure determination such as x-ray diffraction. Atomic resolution...

journal_title：Biopolymers

authors： Nicholson LK,Asakura T,Demura M,Cross TA

更新日期：1993-05-01 00:00:00

abstract：:An electron paramagnetic resonance (EPR) study was performed for potato and wheat starch containing Cu2+ ions as a paramagnetic probe. Distribution of water in the starch granules as well as the interactions between the copper and starch matrix of different crystalline structures were determined. EPR spectra of the na...

journal_title：Biopolymers

authors： Łabanowska M,Bidzińska E,Dyrek K,Szymońska J

更新日期：2006-08-15 00:00:00

abstract：:Inhibitor design against viral targets must take into account the peculiar characteristics of viral biology-in particular, the plasticity of their replicative machinery. This includes maturational cleavage of the polyprotein, which is mediated by virally encoded proteases. Designing against a movable target is particu...

journal_title：Biopolymers

authors： Bianchi E,Pessi A

更新日期：2002-01-01 00:00:00

abstract：:Peptide-conjugated polysaccharide matrices using bioactive laminin-derived peptides are useful biomaterials for tissue and cell engineering. Here, we demonstrate an easy handling preparation method for peptide-polysaccharide matrices using polyion complex with both alginate and chitosan. First, aldehyde-alginate was s...

journal_title：Biopolymers

authors： Fujimori C,Kumai J,Nakamura K,Gu Y,Katagiri F,Hozumi K,Kikkawa Y,Nomizu M

更新日期：2017-01-01 00:00:00

abstract：:We review the neighbor exclusion model and some of its applications to analyze the binding data of DNA-ligand complexes. We revisit the closed form of the model developed by McGhee and von Hippel in 1974, showing that this classic model can be used to help studying the behavior of DNA contour and persistence lengths w...

journal_title：Biopolymers

authors： Rocha MS

更新日期：2010-01-01 00:00:00

abstract：:Peptides based on 2-amino-2,3-dihydro-1H-cyclopenta[b]anthracene-2-carboxylic acid (antAib), a fluorescent, achiral, alpha-amino acid belonging to the class of C(i) (alpha)-->C(i) (alpha) cyclized, C(alpha,alpha)-disubstituted glycines, combined with L-Ala, up to the hexamer level, were synthesized by solution methods...

journal_title：Biopolymers

authors： Wright K,Lohier JF,Wakselman M,Mazaleyrat JP,Peggion C,Formaggio F,Toniolo C

更新日期：2007-01-01 00:00:00

abstract：:G protein-coupled receptor kinase 2 (GRK2) plays a central role in the cellular transduction network. In particular, during chronic heart failure GRK2 is upregulated and believed to contribute to disease progression. Thereby, its inhibition offers a potential therapeutic solution to several pathological conditions. In...

journal_title：Biopolymers

authors： Gomez-Monterrey I,Carotenuto A,Cipolletta E,Sala M,Vernieri E,Limatola A,Bertamino A,Musella S,Grieco P,Trimarco B,Novellino E,Iaccarino G,Campiglia P

更新日期：2014-01-01 00:00:00

abstract：:Ubiquitin (Ub) is a small 76 amino acid long protein that is highly conserved in all eukaryotes studied to date. In humans, more than 600 ligases are involved in the reversible modification of specific lysine side-chain amines in substrate proteins by conjugation with the C-terminal carboxylate of Ub. Initially monoub...

journal_title：Biopolymers

authors： Weller CE,Pilkerton ME,Chatterjee C

更新日期：2014-02-01 00:00:00

abstract：:Through optimization of the linker region and key stabilizing mutations, it has been possible to improve the stability of the circularly permuted (cp) Trp-cage miniprotein. However, even the most stable Trp-cage circular permutants are still less stable than the analogous standard topology (std) Trp-cages. Extending m...

journal_title：Biopolymers

authors： Graham KA,Byrne A,Mason M,Andersen NH

更新日期：2019-12-01 00:00:00

abstract：:Cyclic tri-β-peptide having tetrathiafulvalene (TTF) at the side chain was synthesized to prepare a peptide nanotube aligning TTF side chains along the nanotube. The polarized light microscopic observations revealed crystallization of the cyclic peptide by the vapor diffusion method. Fourier-transform infrared and ele...

journal_title：Biopolymers

authors： Uji H,Kim H,Imai T,Mitani S,Sugiyama J,Kimura S

更新日期：2016-05-01 00:00:00

abstract：:The ability of an exopolymer of glycoproteic character (GP) excreted by a new gram-negative species Pseudoalteromonas antarctica NF(3), to coat phosphatidylcholine (PC) liposomes and to protect these bilayers against the action of the nonionic surfactant dodecyl maltoside was investigated. Transmission electron micros...

journal_title：Biopolymers

authors： de La Maza A,Codech L,Lopez O,Parra JL,Sabes M,Guinea J

更新日期：1999-11-01 00:00:00

abstract：:Photon correlation spectroscopy has been used to study the translational (D0) and internal dynamics of monodisperse linear and polydisperse superhelical plasmid-DNAs. Scattering vector dependent correlation functions were measured and analyzed with the inverse Laplace transform CONTIN written by S. Provencher. For sca...

journal_title：Biopolymers

authors： Seils J,Dorfmüller T

更新日期：1991-06-01 00:00:00