Abstract:
:Porins from outer membrane of Gram-negative bacteria have a highly stable structure. Our previous studies on porin from Paracoccus denitrificans showed that the outer membrane protein porin is extremely stable toward heat, pH, and chemical denaturants. The major question we have addressed in this paper is whether the high stability of porin is a consequence of the beta-barrel structure and whether it is required for its function. To explain this we have analyzed two cases: first, we used porin wild-type and mutants and compared their structure and function; second, we compared the activity of porin preheated to different temperatures. Structural changes were monitored by infrared spectroscopy. We observed that the structural stability of porin is not equivalent to functional activity as minor alteration in the structure can result in drastic differences in the activity of porins.
journal_name
Biopolymersjournal_title
Biopolymersauthors
Sukumaran S,Hauser K,Maier E,Benz R,Mäntele Wdoi
10.1002/bip.20422subject
Has Abstractpub_date
2006-07-01 00:00:00pages
344-8issue
4eissn
0006-3525issn
1097-0282journal_volume
82pub_type
杂志文章相关文献
BIOPOLYMERS文献大全abstract::The mode coupling diffusion theory is applied to the derivation of local dynamics in proteins in solution. The rotational dynamics of the bonds along the protein sequence are calculated and compared to the experimentally measured nmr (15)N spin-lattice relaxation time T(1), at 36.5, 60.8, and 81.1 MHz of the vnd/NK-2 ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/1097-0282(200008)54:2<89::AID-BIP2>3.0.CO;
更新日期:2000-08-01 00:00:00
abstract::The interaction of the immunogenic peptide of human hepatitis B virus (HBV) preS(120-145), including B and T epitopes, with phospholipid vesicles has been studied by fluorescence techniques and CD. In addition, interaction of three lipopeptides derived from preS(120-145) containing stearoyl, cholanoyl, and tripalmitoy...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(sici)1097-0282(199605)38:5<607::aid-bip6>
更新日期:1996-05-01 00:00:00
abstract::Despite the positive results observed in vitro and in vivo, clinical trials with bioactive peptides are generally hampered by their fast degradation in the biological system. Two bioactive peptides, P20 (CSSRTMHHC) and the combined peptide C (CVNHPAFACGYGHTMYYHHYQHHL) have been identified as anticancer therapeutics. C...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.23029
更新日期:2017-09-01 00:00:00
abstract::The phase content of amylose-water mixtures (0.7/0.3 w/w) has been analyzed by transmission Fourier transform infrared (FTIR) spectroscopy in the 1175-950 cm(-1) region. Spectra are obtained under three different conditions: (a) as a function of temperature (T) from 25 to 97 degrees C; (b) at room temperature (RT), af...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/1097-0282(200103)58:3<305::AID-BIP1007>3.0
更新日期:2001-03-01 00:00:00
abstract::Optical melting curves of 22 DNA dumbbells with the 16-base pair duplex sequence 5'-G-C-A-T-C-A-T-C-G-A-T-G-A-T-G-C-3' linked on both ends by single-strand loops of A, or C, sequences (iota = 2, 3, 4, 6, 8, 10, 14). T sequences (iota = 2, 3, 4, 6, 8, 10), and G iota sequences (iota = 2, 4) were measured in phosphate b...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0282(199612)39:6%3C779::AID-BIP
更新日期:1996-12-01 00:00:00
abstract::Recombinant murine amelogenins M179 and M166 were expressed in Escherichia coli and purified. The aggregation properties of these amelogenins have been investigated in aqueous solutions as well as acetonitrile-containing solutions using dynamic light scattering. Dynamic light scattering provides direct measurement of ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360341006
更新日期:1994-10-01 00:00:00
abstract::Caspases comprise a family of dimeric cysteine proteases that control apoptotic programmed cell death and are therefore critical in both organismal development and disease. Specific inhibition of individual caspases has been repeatedly attempted, but has not yet been attained. Caspase-9 is an upstream or initiator cas...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22080
更新日期:2012-01-01 00:00:00
abstract::Over the past decade a large number of studies have focused attention on the role of nucleosomes as negative and positive regulators of specific nuclear functions. Due to the lack of an analytical method to determine the higher order conformation of the nucleosomal arrays that encompass specific genetic loci (e.g., pr...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10322
更新日期:2003-04-01 00:00:00
abstract::Recently, the use of hybrid double network (DN) hydrogels has become prominent due to their enhanced mechanical properties, which has opened the door for new applications of these soft materials. Only a few of these gels have demonstrated both injectable and moldable capabilities. In this work, we report the mechanica...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.23355
更新日期:2020-06-01 00:00:00
abstract::The applicability of the FTIR attenuated total reflectance technique for in situ monitoring of plant physiological processes such as leaf senescence and aging has been examined. Difference spectra obtained by subtracting the spectrum of the young plant leaf from that of the older one revealed positive bands at 1650-15...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10297
更新日期:2003-01-01 00:00:00
abstract::As a model of receptor protein, a series of 3alpha-helix bundle peptides constructed on a template peptide were designed so as to possess a hydrophobic cavity. The size of cavity was modulated by simple replacements of Leu residues to Ala residues in the hydrophobic core. Binding abilities to 8-anilino-1-naphthalenesu...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/1097-0282(200108)59:2<65::AID-BIP1006>3.0.
更新日期:2001-08-01 00:00:00
abstract::The Electrostatically Driven Monte Carlo (EDMC) method was applied in a study of a decamer of glycine whose conformational behavior is described by the Empirical Conformational Energy Program for Peptides (ECEPP/2) potential energy model. When free neutral end groups were used, it was found that conformations that wer...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360310306
更新日期:1991-02-15 00:00:00
abstract::We review the neighbor exclusion model and some of its applications to analyze the binding data of DNA-ligand complexes. We revisit the closed form of the model developed by McGhee and von Hippel in 1974, showing that this classic model can be used to help studying the behavior of DNA contour and persistence lengths w...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.21296
更新日期:2010-01-01 00:00:00
abstract::Multiconfiguration thermodynamic integration was used to determine the relative binding strength of tacrine and 6-chlorotacrine by Torpedo californica acetylcholinesterase. 6-Chlorotacrine appears to be bound stronger by 0.7+/-0.4 kcal/mol than unsubstituted tacrine when the active site triad residue His-440 is deprot...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(sici)1097-0282(199601)38:1<109::aid-bip9>
更新日期:1996-01-01 00:00:00
abstract::The 83-residue conopeptide (p21a) and its corresponding nonhydroxylated analog were isolated from the injected venom of Conus purpurascens. The complete conopeptide sequences were derived from Edman degradation sequencing of fragments from tryptic, chymotryptic and cyanogen bromide digestions, p21a has a unique, 10-cy...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21406
更新日期:2011-01-01 00:00:00
abstract::CD and nmr spectroscopy were used to compare the conformational properties of two related peptides. One of the peptides, Model AB, was designed to adopt a helix-turn-extended strand (alpha beta) tertiary structure in water that might be stabilized by hydrophobic interactions between two leucine residues in the amino-t...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360360202
更新日期:1995-08-01 00:00:00
abstract::The X-ray diffraction analysis of a stereocontrolled heterochiral designed model peptide Boc-(D) Pro-Thr-OMe (1) revealed the existence of an unusual folded molecular structure, stabilized via an effective unconventional C---H…O type intramolecular hydrogen-bond, encompassing a noncovalent 12-membered ring-motif. Toge...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21705
更新日期:2012-01-01 00:00:00
abstract::Molecular modeling is used to demonstrate that the major structural deformations of DNA caused by four different minor groove binding proteins, TBP, SRY, LEF-1, and PurR, can all be mimicked by stretching the double helix between two 3'-phosphate groups flanking the binding region. This deformation reproduces the wide...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0282(19990415)49:5<341::AID-BIP
更新日期:1999-04-15 00:00:00
abstract::An increasing number of RNAs are being discovered that perform their functions by undergoing large changes in conformation in response to a variety of cellular signals, including recognition of proteins and small molecular targets, changes in temperature, and RNA synthesis itself. The measurement of NMR residual dipol...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.20765
更新日期:2007-08-05 00:00:00
abstract::Collagen is a natural protein, which is used as a vital biomaterial in tissue engineering. The major concern about native collagen is lack of its thermal stability and weak resistance to proteolytic degradation. In this scenario, the crosslinking compounds used for stabilization of collagen are mostly of chemical natu...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22473
更新日期:2014-08-01 00:00:00
abstract::The IgY antibodies offer an attractive alternative to mammalian IgGs in research, diagnosis and medicine. The isolation of immunoglobulin Y from the egg yolks is efficient and economical, causing minimal suffering to animals. Here we present the methodology for the production of IgY antibodies specific to Staphylococc...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22695
更新日期:2015-09-01 00:00:00
abstract::Cytochrome c3 from Desulfovibrio gigas is electrostatically adsorbed on Ag electrodes coated with self-assembled monolayers (SAMs) of 11-mercaptoundecanoic acid. The redox equilibria and electron transfer dynamics of the adsorbed four-heme protein are studied by surface enhanced resonance Raman spectroscopy. Immobiliz...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10101
更新日期:2002-01-01 00:00:00
abstract::Recent advances in the application of solid state nmr spectroscopy to uniformly aligned biopolymers have opened a window through which to view the detailed structure of biological macromolecules that are unable to be seen with standard techniques for structure determination such as x-ray diffraction. Atomic resolution...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360330513
更新日期:1993-05-01 00:00:00
abstract::The spatial variation in the composition of nonexpanded biopolymer blends prepared by extrusion of mixtures of gelatin with either native or pregelatinized waxy maize starch was studied using a 30-microm aperture FTIR microspectroscopy technique. The ratio of the areas of the "saccharide" bands (953-1180 cm(-1)) and t...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.1015
更新日期:2001-01-01 00:00:00
abstract::The comprehensive understanding of disassembly mechanism of amyloid fibrils requires nano-scale characterization of the mechanical properties of amyloid fibrils during the disassembly process. In this work, gemini surfactant C12 C6 C12 Br2 micelles were used as a probe to disassemble Aβ(1-40) fibrils. The microstructu...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22992
更新日期:2017-02-01 00:00:00
abstract::The secondary structure of a 38 kDa core protein from pig skin proteodermatan sulfate (PDS), was investigated in solution using CD and Fourier transform (FT) ir spectroscopy. Both techniques generally have provided complementary data on the secondary structures of proteins. CD spectral analysis has shown that the core...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360281109
更新日期:1989-11-01 00:00:00
abstract::Peptide recognition by G-protein coupled receptors (GPCRs) is reviewed with an emphasis on the indirect approach used to determine the receptor-bound conformation of peptide ligands. This approach was developed in response to the lack of detailed structural information available for these receptors. Recent advances in...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/1097-0282(2001)60:3<246::AID-BIP10044>3.0.
更新日期:2001-01-01 00:00:00
abstract::High-level quantum-chemical and quantum-dynamics calculations are reported on the tautomerization equilibrium and rate constants of guanine and its complexes with one and two water molecules. The results are used to estimate the fraction of guanine present in the cell during DNA synthesis as the unwanted tautomer that...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/1097-0282(2001)61:1<77::AID-BIP10062>3.0.C
更新日期:2001-01-01 00:00:00
abstract::Cyclic pentapeptides are excellent models for reverse turns and have been used extensively in our laboratory to explore the influence of different amino acid sequences on turn preference. This paper is divided into two parts: In the first, we review our previous studies of cyclic pentapeptides. We summarize work that ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360290130
更新日期:1990-01-01 00:00:00
abstract::The RNA recognition motif (RRM) is one of the most common RNA binding domains. We have investigated the contribution of three highly conserved aromatic amino acids to RNA binding by the N-terminal RRM of the U1A protein. Recently, we synthesized a modified base (A-4CPh) in which a phenyl group is tethered to adenine u...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20408
更新日期:2006-03-01 00:00:00