Abstract:
:The applicability of the FTIR attenuated total reflectance technique for in situ monitoring of plant physiological processes such as leaf senescence and aging has been examined. Difference spectra obtained by subtracting the spectrum of the young plant leaf from that of the older one revealed positive bands at 1650-1500 cm(-1), indicating a higher relative concentration of phenolics in the older leaves of both black cherry and sweet pepper bush leaves. Prolonged physiological stress of tobacco leaves exhibited a progressive time-dependent increase of the absorbance at around 3475 cm(-1), corresponding to hydroxyl functional groups. Absorption changes were also observed between 1650 and 1500 cm(-1), which are likely to correspond to phenolics. The characteristic changes of the FTIR absorbance spectra resulting from physiological and induced aging were detected also as a response to treatment with a recombinant alpha-elicitin, cinnamomin. This allowed the first quantification of the biological activity of a recombinant elicitin using a spectroscopic method. We suggest that FTIR spectroscopy provides important information about physiological events occurring in plant tissue in vivo, and it could be useful for the in situ characterization of the plant responsiveness to fungal toxins such as elicitins.
journal_name
Biopolymersjournal_title
Biopolymersauthors
Ivanova DG,Singh BRdoi
10.1002/bip.10297subject
Has Abstractpub_date
2003-01-01 00:00:00pages
79-85issue
2eissn
0006-3525issn
1097-0282journal_volume
72pub_type
杂志文章相关文献
BIOPOLYMERS文献大全abstract::TACC3 is a centrosomal adaptor protein that plays important roles during mitotic spindle assembly. It interacts with chTOG/XMAP215, which catalyzes the addition of tubulin dimers during microtubule growth. A 3D coiled-coil model for this interaction is available but the structural details are not well described. To ch...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.23039
更新日期:2017-11-01 00:00:00
abstract::The large abundance of bioactive single- and multiple-stranded cystine-rich peptides in nature has fostered the development of orthogonal thiol-protection schemes and of efficient chemistries for regioselective disulfide formation in synthetic replica for decades. In parallel to these entirely synthetic strategies, an...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.20174
更新日期:2005-01-01 00:00:00
abstract::Fe(III)-Mimochrome VI (MC6) is a recently reported artificial heme-peptide conjugate system with a high peroxidase-like activity. By design, its structure features a five-coordinated Fe(III)-deuteroporphyrin active site, embedded in a compact α-helix-heme-α-helix "sandwich" motif. Up to now, no detailed MC6 structural...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.23225
更新日期:2018-08-01 00:00:00
abstract::To investigate the mechanism leading to the reduction in tensile strength of permanent waved human hair, the structure of cross-sections at various depths of permanent waved white human hair was directly analyzed without isolating the cuticle and cortex, using Raman spectroscopy. The beta-sheet and/or random coil cont...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20646
更新日期:2007-02-15 00:00:00
abstract::In our continuing series of measurements of the complex index of refraction for representative samples of biological materials, we measured ovalbumin (egg albumin) over the spectral region from 0.130 (76,923 cm(-1)) to 2.50 microm (4000 cm(-1)). Films of ovalbumin suitable for optical analyses were prepared and measur...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.1004
更新日期:2001-01-01 00:00:00
abstract::We analyzed the CD and uv absorption spectra of 5S RNA from Escherichia coli using the method developed in the preceding paper. The analysis of spectra of 5S RNA at 20 degrees C in 0.1M NaClO4, 2.5 mM Na+ (phosphate), pH 7.0, and 0.5 mM MgSO4 gave 7 +/- 3.6 A.U base pairs, 25 +/- 3.6 G.C base pairs, and 7.5 +/- 3.6 G....
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360310404
更新日期:1991-03-01 00:00:00
abstract::For mimicking macromolecular crowding of DNA quadruplexes, various crowding agents have been used, typically PEG, with quadruplexes of micromolar strand concentrations. Thermal and thermodynamic stabilities of these quadruplexes increased with the concentration of the agents, the rise depended on the crowder used. A d...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22400
更新日期:2014-04-01 00:00:00
abstract::Inhibitor design against viral targets must take into account the peculiar characteristics of viral biology-in particular, the plasticity of their replicative machinery. This includes maturational cleavage of the polyprotein, which is mediated by virally encoded proteases. Designing against a movable target is particu...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.10230
更新日期:2002-01-01 00:00:00
abstract::Cyclic tri-β-peptide having tetrathiafulvalene (TTF) at the side chain was synthesized to prepare a peptide nanotube aligning TTF side chains along the nanotube. The polarized light microscopic observations revealed crystallization of the cyclic peptide by the vapor diffusion method. Fourier-transform infrared and ele...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22850
更新日期:2016-05-01 00:00:00
abstract::The design of modified nucleic acid aptamers is improved by considering thermodynamics and kinetics of their association/dissociation processes. Locked Nucleic Acids (LNA) is a promising class of nucleic acid analogs. In this work the thermodynamic and kinetic properties of a LNA quadruplex formed by the TGGGT sequenc...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20591
更新日期:2006-12-15 00:00:00
abstract::DNA sequences containing four types of analog nucleosides are described. All four are pyridine derivatives constructed as C-nucleosides so that they mimic the pyrimidine derivatives 2'-deoxyuridine, thymidine or 2'-deoxycytidine, but in all cases the analogs lack the corresponding O2-carbonyls that in duplex DNA are l...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20817
更新日期:2007-10-05 00:00:00
abstract::Neurokinins and their receptors are a complex system consisting of at least three endogenous agents--substance P (SP), neurokinin A (NKA), and neurokinin B (NKB)--and their corresponding receptor types, respectively, NK-1, NK-2, and NK-3. Investigations on receptors have been made using sensitive and fairly selective ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360280111
更新日期:1989-01-01 00:00:00
abstract::Porins from outer membrane of Gram-negative bacteria have a highly stable structure. Our previous studies on porin from Paracoccus denitrificans showed that the outer membrane protein porin is extremely stable toward heat, pH, and chemical denaturants. The major question we have addressed in this paper is whether the ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20422
更新日期:2006-07-01 00:00:00
abstract::Dynamic and static intensity light scattering techniques were employed to identify conditions allowing preparation of homogeneous solutions of distinct oligomeric states of RecA protein. These hydrodynamically distinguishable oligomer populations of RecA protein were obtained in homogeneous pure quantities sufficient ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(sici)1097-0282(199604)38:4<471::aid-bip4>
更新日期:1996-04-01 00:00:00
abstract::Nocistatin, a new heptadecapeptide encoded in the bPNP-3 gene, has a powerful biological activity connected with the mechanisms of pain transmission. It does not bind to the opioid receptors but to another brain receptor with high affinity. In order to substantiate these novel biological data with a structural basis, ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0282(200003)53:3<257::AID-BIP5>
更新日期:2000-03-01 00:00:00
abstract::The open reading frame 4 (ORF 4) gene product of barley yellow dwarf virus (BYDV) may act as a movement protein (MP) by assisting the transport of viral genomic RNA across the nuclear envelope (NE) of host plant cells. To investigate interactions between BYDV MP and the NE, wild-type and mutant open reading frame (ORF...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20334
更新日期:2005-10-05 00:00:00
abstract::Amyloid aggregates have been implicated in the pathogenesis of diseases such as type 2 diabetes, Alzheimer's, Parkinson's, and prion disease. Recently determined microcrystal structures of several short peptide segments derived from fibril-forming proteins revealed coexistence of alternative aggregation modes (amyloid...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21731
更新日期:2012-01-01 00:00:00
abstract::The conformation of a 13-residue C-peptide analogue of ribonuclease A, suc-AET-AAAKFLRAHA-CONH2, in NaDodSO4 solutions with respect to temperature was studied with CD. The equilibrium constant of unfolding yielded a straight line in a van't Hoff plot. In 10 mM NaDodSO4, delta G mu = 120 cal/mol, delta H mu = 700 cal/m...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360300315
更新日期:1990-01-01 00:00:00
abstract::The interaction of natural calf thymus DNA with Cr(3+) ions was studied at room temperature by means of vibrational CD (VCD) and infrared absorption (ir) spectroscopy, and atomic force microscopy (AFM). Cr(3+) ion binding mainly to N(7) (G) and to phosphate groups was demonstrated. Psi-type VCD spectra resembling elec...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10159
更新日期:2001-01-01 00:00:00
abstract::Combinatorial library composed of rigid rod-like peptides with a triple-helical scaffold was constructed. The component peptides were designed to have various combinations of basic and neutral (or hydrophobic) amino acid residues based on collagen-like (Gly-Pro-Yaa)-repeating sequences, inspired from the basic and amp...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22791
更新日期:2016-11-04 00:00:00
abstract::Bovine carbonmonoxy hemoglobin investigated with light scattering studies is found to dissociate from its native tetramer structure into dimers and monomers. The values of the hydrated tetramer radius, RT = 32.1 A, and the fractional dissociation vs pH, have been obtained at different ionic strengths from the autocorr...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360340607
更新日期:1994-06-01 00:00:00
abstract::The results are presented for the deconvolution of IR spectra of disaccharides and polysaccharides with alpha and beta configurations of the 1 --> 4 glycosidic linkage (maltose, cellobiose, amylose, and cellulose), as well as of their corresponding monosaccharides (alpha- and beta-D-glucose) in the 1200-920 cm(-1) fre...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/1097-0282(2000)57:4<257::AID-BIP7>3.0.CO;2
更新日期:2000-01-01 00:00:00
abstract::Vibrational spectroscopy coupled with thermogravimetry (TG) and differential scanning calorimetry (DSC) was used to characterize racemic propanoic acid, 2-methyl-5,6,7,8-tetrahydro-6-(methylamino)-1,2-naphthalenediyl ester hydrochloride (CHF-1035), which is a new DA2 dopaminergic receptor/alpha2 agonist and beta block...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10089
更新日期:2002-01-01 00:00:00
abstract::Differential scanning calorimetry measurements performed on RNase A in aqueous binary solutions containing different concentrations of urea, tetramethylurea, guanidinium chloride, and guanidinium thiocyanate, and in aqueous ternary solutions, containing the same denaturants plus 1 M trimethylamine N-oxide, TMAO, demon...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.23104
更新日期:2018-08-01 00:00:00
abstract::Using a variety of spectroscopic techniques, a number of molecular functionalities have been studied in relation to the adsorption process of proteins to air-water interfaces. While ellipsometry and drop tensiometry are used to derive information on adsorbed amount and exerted surface pressure, external reflection cir...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20036
更新日期:2004-05-01 00:00:00
abstract::The spectroscopic properties of syringomycin E, an antibiotic lipodepsinonapeptide associated with pathological states in plants, have been investigated by uv absorbance and CD spectroscopies, and by the synthesis of relevant model compounds. Initial studies [E. Vaillo, A. Ballio, P. L. Luisi, and R. M. Thomas (1990) ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360321006
更新日期:1992-10-01 00:00:00
abstract::The preferred helical screw senses of chiral α-amino acids with a C(α)-tetrasubstituted α-carbon atom, as determined in the crystal state by X-ray diffraction analyses on derivatives and peptides, are reviewed. This survey covers C(α)-methylated and C(α)-ethylated α-amino acids, as well as α-amino acids cyclized on th...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22581
更新日期:2015-01-01 00:00:00
abstract::The existence of an "RNA world" as an early step in the history of life increases the interest for the characterization of these biomolecules. The hairpin ribozyme studied here is a self-cleaving/ligating motif found in the minus strand of the satellite RNA associated with Tobacco ringspot virus. Surface-enhanced Rama...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21143
更新日期:2009-05-01 00:00:00
abstract::The presence of non-native kinetic traps in the free energy landscape of a protein may significantly lengthen the overall folding time so that the folding process becomes unreliable. We use a computational model alpha-helical hairpin peptide to calculate structural free energy landscapes and relate them to the kinetic...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20388
更新日期:2006-02-15 00:00:00
abstract::Vibrational absorption and vibrational circular dichroism (VCD) spectra of valinomycin are measured, in different solvents, in the ester and amide carbonyl stretching regions. The influence of cations, namely Li(+), Na(+), K(+), and Cs(+), in methanol-d(4) solvent is also investigated. Ab initio quantum mechanical cal...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20103
更新日期:2004-09-01 00:00:00