Binding of tacrine and 6-chlorotacrine by acetylcholinesterase.

abstract：:The quantum yield for cyclobutyl-pyrimidine dimerization in DNA has been observed to increase approximately linearly with increasing pyrimidine tract length. A model without adjustable parameters for this yield is proposed based on energy delocalization, vibronic symmetry switching, and saturation statistics that desc...

journal_title：Biopolymers

authors： Texter J

更新日期：1992-01-01 00:00:00

abstract：:The oxidation of methionine to methionine sulfoxide both in vivo and in vitro can lead to the loss of biological activity in a variety of proteins. This loss of activity can be reversed by an enzyme called methionine sulfoxide reductase. The gene for this enzyme has been cloned and sequenced from a variety of prokaryo...

journal_title：Biopolymers

authors： Brot N,Weissbach H

更新日期：2000-01-01 00:00:00

abstract：:Terminally blocked (L-Pro-Aib)n and Aib-(L-Pro-Aib)n sequential oligopeptides are known to form right-handed beta-bend ribbon spirals under a variety of experimental conditions. Here we describe the results of a complete CD and ir characterization of this subtype of 3(10)-helical structure. The electronic CD spectra w...

journal_title：Biopolymers

authors： Yoder G,Keiderling TA,Formaggio F,Crisma M,Toniolo C

更新日期：1995-01-01 00:00:00

abstract：:Two-dimensional nmr spectra [correlated spectroscopy (COSY), homonuclear Hartmann-Hahn (HOHAHA), nuclear Overhauser effect spectroscopy (NOESY)] have been observed for cross-linked lysozyme, a chemically modified lysozyme derivative with an extra ester cross-link between residues E35 and W108. Eight shifted cross-peak...

journal_title：Biopolymers

authors： Noda Y,Fukuda Y,Segawa S

更新日期：1997-02-01 00:00:00

abstract：:In the past two decades, keratin biomaterials have shown impressive results as scaffolds for tissue engineering, wound healing, and nerve regeneration. In addition to its intrinsic biocompatibility, keratin interacts with specific cell receptors eliciting beneficial biochemical cues. However, during extraction from na...

journal_title：Biopolymers

authors： Parker RN,Roth KL,Kim C,McCord JP,Van Dyke ME,Grove TZ

更新日期：2017-10-01 00:00:00

abstract：:DMT1, also known as Nramp2, is an iron transporter, and belongs to the family of Nramp proteins. Disease-causing mutations both in Nramp1 and Nramp2 occurring at the conserved two adjacent glycine residues located within the fourth transmembrane domain (TM4) suggest that TM4 may serve an important biological function....

journal_title：Biopolymers

authors： Li H,Li F,Kwan M,He QY,Sun H

更新日期：2005-03-01 00:00:00

abstract：:The aim of this study was to develop a nondestructive method to quantitate relative amounts of n-3 and n-6 polyunsaturated fatty acid (PUFA) species in vegetable oils and oil seeds using Fourier transform IR spectroscopy (FTIR). The alkene Cbond;H stretching vibrations of unsaturated fatty acids in oils showed IR abso...

journal_title：Biopolymers

authors： Yoshida S,Yoshida H

更新日期：2003-12-01 00:00:00

abstract：:Seven water-insoluble (1 --> 3)-beta-D-glucan fractions TM8-1 to TM8-7 with weight-average molecular mass M(w) ranged from 2.22 to 77.4 x 10(4) obtained from the sclerotia of Pleurotus tuber-regium were carboxymethylated to produce the water-soluble fractions CTM8-1 to CTM8-7 with M(w) ranged from 3.87 to 87.8 x 10(4)...

journal_title：Biopolymers

authors： Zhang M,Zhang L,Cheung PC

更新日期：2003-02-01 00:00:00

abstract：:Partially acetylated, high molecular weight glucuronans were produced by a Sinorhizobium meliloti mutant strain. Two native glucuronan samples with various degrees of acetylation were sonicated to obtain lower molecular weight samples and with low viscosity suitable for chemical modification and (13)C NMR experiments....

journal_title：Biopolymers

authors： Pau-Roblot C,Petit E,Sarazin C,Courtois J,Courtois B,Barbotin JN,Séguin JP

更新日期：2002-06-01 00:00:00

abstract：:Fluorescein-labeled antibodies are widely used in clinical assays and fluorescence microscopy. The fluorescent signal per labeled antibody is limited by fluorescein self-quenching, which occurs when the antibody is heavily labeled with multiple fluoresceins. We examined immunoglobulin G (IgG) when labeled with 0.7 to ...

journal_title：Biopolymers

authors： Lakowicz JR,Malicka J,Huang J,Gryczynski Z,Gryczynski I

更新日期：2004-08-15 00:00:00

abstract：:The presence of non-native kinetic traps in the free energy landscape of a protein may significantly lengthen the overall folding time so that the folding process becomes unreliable. We use a computational model alpha-helical hairpin peptide to calculate structural free energy landscapes and relate them to the kinetic...

journal_title：Biopolymers

authors： Chapagain PP,Gerstman BS

更新日期：2006-02-15 00:00:00

abstract：:Infrared spectra of the amino acid residues in H2O solution have been obtained in the 1800-1400-cm-1 region. It has been established that amino acid residues of arginine, asparagine, glutamine, aspartic and glutamic acids, lysine, tyrosine, histidine, and phenylalanine have intensive absorption in this spectral region...

journal_title：Biopolymers

authors： Venyaminov SYu,Kalnin NN

更新日期：1990-01-01 00:00:00

abstract：:We analyzed the CD and uv absorption spectra of 5S RNA from Escherichia coli using the method developed in the preceding paper. The analysis of spectra of 5S RNA at 20 degrees C in 0.1M NaClO4, 2.5 mM Na+ (phosphate), pH 7.0, and 0.5 mM MgSO4 gave 7 +/- 3.6 A.U base pairs, 25 +/- 3.6 G.C base pairs, and 7.5 +/- 3.6 G....

journal_title：Biopolymers

authors： Johnson KH,Gray DM

更新日期：1991-03-01 00:00:00

abstract：:We describe here a new method for determining the conformational stability of antiparallel beta-sheets. Due to coupling between the transition dipoles, beta-sheet conformations typically exhibit a characteristic high-frequency amide I component centered at approximately 1680 cm(-1). Using one beta-sheet protein and tw...

journal_title：Biopolymers

authors： Wang T,Xu Y,Du D,Gai F

更新日期：2004-10-05 00:00:00

abstract：:Peptides are frequently used model systems for protein folding. They are also gaining increased importance as therapeutics. Here, the ability of molecular dynamics (MD) simulation for describing the structure and dynamics of β-hairpin peptides was investigated, with special attention given to the impact of a single in...

journal_title：Biopolymers

authors： Niebling S,Danelius E,Brath U,Westenhoff S,Erdélyi M

更新日期：2015-11-01 00:00:00

abstract：:Raman spectra of series of aqueous solutions of peptides containing two amino acids, glycine-X, alanine-X, and serine-X, where X is an uncharged amino acid, show that the amide III band shifts systematically to lower frequencies as the side chain of the X amino acid becomes larger. The range of this shift is about 20 ...

journal_title：Biopolymers

authors： Weaver JL,Williams RW

更新日期：1990-01-01 00:00:00

abstract：:Deoxyribozyme and aptamer selections are typically conducted in aqueous buffer solutions. Using nonaqueous cosolvents in selection experiments will help expand the activity of deoxyribozymes with non-oligonucleotide substrates and will allow identification of new aptamers for nonprotein targets. We undertook in vitro ...

journal_title：Biopolymers

authors： Behera AK,Schlund KJ,Mason AJ,Alila KO,Han M,Grout RL,Baum DA

更新日期：2013-06-01 00:00:00

abstract：:Molecular dynamics simulation, with backbone constraints for 20 ps of equilibration and simulation, of a repeating polypeptide segment, Gln-Pro-His-Gln-Pro-Leu-Gln-Pro-His-Gln-Pro-Leu-Gln-Pro-Met-(Gln-Pro-Leu )4, constituting residues 112-138 of bovine amelolgenin, a 19.35 kD hydrophobic protein, are discussed. It is ...

journal_title：Biopolymers

authors： Renugopalakrishnan V,Pattabiraman N,Prabhakaran M,Strawich E,Glimcher MJ

更新日期：1989-01-01 00:00:00

abstract：:Fourteen tryptic peptides and nine intermediates were identified as products of trypsin digestion of reduced and S-3-(trimethylated amino) propylated lysozyme. Kinetics of the appearance and disappearance of these products were observed by monitoring the peak areas on the chromatogram. In spite of the complicated reac...

journal_title：Biopolymers

authors： Noda Y,Fujiwara K,Yamamoto K,Fukuno T,Segawa S

更新日期：1994-02-01 00:00:00

abstract：:Synthesis and CD and (13)C(alpha)-NMR studies in a near-neutral saline buffer are reported for a 65-residue peptide ((190)Tm(254)) comprising residues 190-254 of the alpha-tropomyosin chain. CD on a version disulfide cross-linked via the N-terminal cysteine side chains indicates that this dimer is highly helical and m...

journal_title：Biopolymers

authors： Emerson Holtzer M,Mints L,Hogue Angeletti R,d'Avignon DA,Holtzer A

更新日期：2001-10-05 00:00:00

abstract：:Conformational analysis of the neurohypophyseal hormones oxytocin (OT) and arginine-vasopressin (AVP) has been carried out using two different computational approaches and three force fields, namely by the Electrostatically Driven Monte Carlo (EDMC) method, with the Empirical Conformational Energy Program for Peptides...

journal_title：Biopolymers

authors： Liwo A,Tempczyk A,Ołdziej S,Shenderovich MD,Hruby VJ,Talluri S,Ciarkowski J,Kasprzykowski F,Lankiewicz L,Grzonka Z

更新日期：1996-02-01 00:00:00

abstract：:Differential scanning calorimetry measurements performed on RNase A in aqueous binary solutions containing different concentrations of urea, tetramethylurea, guanidinium chloride, and guanidinium thiocyanate, and in aqueous ternary solutions, containing the same denaturants plus 1 M trimethylamine N-oxide, TMAO, demon...

journal_title：Biopolymers

authors： Vigorita M,Cozzolino S,Oliva R,Graziano G,Del Vecchio P

更新日期：2018-08-01 00:00:00

abstract：:The characterization of elastic properties of biopolymers is crucial to understand many molecular reactions determined by conformational bending fluctuations of the polymer. Direct measurement of such elastic properties using single-molecule methods is usually hindered by the intrinsic tendency of such biopolymers to ...

journal_title：Biopolymers

authors： Alemany A,Ritort F

更新日期：2014-12-01 00:00:00

abstract：:The interhelical interfaces have been examined in seven high-resolution globin chains. The profiles of hydrophobic contact, as measured by the residue solvent-accessible area loss upon folding, have been calculated. The seven globins studied differ in their overall loss of solvent-accessible area upon packing of their...

journal_title：Biopolymers

authors： Weaver DL

更新日期：1992-05-01 00:00:00

abstract：:This paper is a personal recollection of some of the events and research that surrounded the amino acid sequence determination of the hypothalamic releasing factor, TRF (now known as TRH), by mass spectrometry (MS), and the development of reverse phase high performance liquid chromatography (RP-HPLC) MS and tandem MS ...

journal_title：Biopolymers

authors： Desiderio DM

更新日期：1996-01-01 00:00:00

abstract：:In order to check our current knowledge on the principles involved in beta-hairpin formation, we have modified the sequence of a 3:5 beta-hairpin forming peptide with two different purposes, first to increase the stability of the formed 3:5 beta-hairpin, and second to convert the 3:5 beta-hairpin into a 2:2 beta-hairp...

journal_title：Biopolymers

authors： Santiveri CM,Pantoja-Uceda D,Rico M,Jiménez MA

更新日期：2005-10-15 00:00:00

abstract：:The methods used to study the relative reaction rates of 45 different aliphatic and aromatic carboxylic acids when coupled to resin-bound amino acid amides is described. Competition experiments involving the coupling of incoming carboxylic acids to resin-bound amino acid amides were performed. The relative composition...

journal_title：Biopolymers

authors： Acharya AN,Ostresh JM,Houghten RA

更新日期：2002-10-05 00:00:00

abstract：:Pig citrate synthase (PCS) can be used as a model enzyme to gain some insight into the structural basis of protein thermostability. The thermal unfolding characteristics of the specific secondary structure elements within PCS were monitored in detail by following changes in its amide I band components. The result of o...

journal_title：Biopolymers

authors： Severcan F,Haris PI

更新日期：2003-08-01 00:00:00

abstract：:We have determined using NMR the structure of the complex between the third double-stranded RNA-binding domain (dsRBD3) of Drosophila Staufen protein and a RNA stem-loop with optimal binding properties in vitro. This work was designed to understand how dsRBD proteins bind RNA and to investigate the role of Staufen dsR...

journal_title：Biopolymers

authors： Ramos A,Bayer P,Varani G

更新日期：1999-01-01 00:00:00

abstract：:Spin-spin and spin-lattice 1H-nmr relaxation times of the sea anemone polypeptide anthopleurin-A were measured at frequencies of 200, 300, 400, and 500 MHz. Relaxation times were fitted iteratively by least squares regression to the isotropic tumbling model, Woessner's model for anisotropic motion, and Lipari and Szab...

journal_title：Biopolymers

authors： Torda AE,Norton RS

更新日期：1989-03-01 00:00:00