Abstract:
:Partially acetylated, high molecular weight glucuronans were produced by a Sinorhizobium meliloti mutant strain. Two native glucuronan samples with various degrees of acetylation were sonicated to obtain lower molecular weight samples and with low viscosity suitable for chemical modification and (13)C NMR experiments. The average degree of substitution (DS) of the polymer was estimated by Fourier transform infrared (FTIR) and NMR. (13)C NMR spectra were obtained and used to suggest a complete assignment of the signals. The nuclear Overhauser effect spectroscopy (NOESY) and heteronuclear multi-bond coherence (HMBC) experiments were used to elucidate connectivities between the various residues and deduce the linkage of these residues within the polysaccharide.
journal_name
Biopolymersjournal_title
Biopolymersauthors
Pau-Roblot C,Petit E,Sarazin C,Courtois J,Courtois B,Barbotin JN,Séguin JPdoi
10.1002/bip.10134subject
Has Abstractpub_date
2002-06-01 00:00:00pages
34-43issue
1eissn
0006-3525issn
1097-0282pii
10.1002/bip.10134journal_volume
64pub_type
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