Abstract:
:Terminally blocked (L-Pro-Aib)n and Aib-(L-Pro-Aib)n sequential oligopeptides are known to form right-handed beta-bend ribbon spirals under a variety of experimental conditions. Here we describe the results of a complete CD and ir characterization of this subtype of 3(10)-helical structure. The electronic CD spectra were obtained in solvents of different polarity in the 260-180 nm region. The vibrational CD and Fourier transform ir (FTIR) spectra were measured in deuterochloroform solution in the amide I and amide II (1750-1500 cm-1) regions. The critical chain length for full development of the beta-bend ribbon spiral structure is found to be five to six residues. Spectral effects related to concentration-induced stabilization of the structures of the longer peptides were seen in the resolution-enhanced FTIR spectra. Comparison to previous studies of (Aib)n and (Pro)n oligomers indicate that the low frequency of the amide I mode is due to the interaction of secondary and tertiary amide bonds and not to a strong difference in conformation from a regular 3(10)-helix.
journal_name
Biopolymersjournal_title
Biopolymersauthors
Yoder G,Keiderling TA,Formaggio F,Crisma M,Toniolo Cdoi
10.1002/bip.360350111subject
Has Abstractpub_date
1995-01-01 00:00:00pages
103-11issue
1eissn
0006-3525issn
1097-0282journal_volume
35pub_type
杂志文章相关文献
BIOPOLYMERS文献大全abstract::Molecular structures for parallel DNA and RNA double helices with Hoogsteen pairing are proposed for the first time. The DNA helices have sugars in the C2'-endo region and the phosphodiester conformations are (trans, gauche-), and the RNA helices have sugars in the C3'-endo region and the phosphodiester conformations ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360341202
更新日期:1994-12-01 00:00:00
abstract::Synthetic RNA poly[r(A-T)] has been synthesized and its CD spectral properties compared to those of poly[r(A-U)], poly[d(A-T)], and poly[d(A-U)] in various salt and ethanolic solutions. The CD spectra of poly[r(A-T)] in an aqueous buffer and of poly[d(A-T)] in 70.8% v/v ethanol are very similar, suggesting that they b...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360290210
更新日期:1990-02-05 00:00:00
abstract::The role of phosphorylation in stabilizing the N-termini of alpha-helices is examined using computer simulations of model peptides. The models comprise either a phosphorylated or unphosphorylated serine at the helix N-terminus, followed by nine alanines. Monte Carlo/stochastic Dynamics simulations were performed on th...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0282(199903)49:3<225::AID-BIP4>
更新日期:1999-03-01 00:00:00
abstract::Regulatory, receptor-binding peptides are considered as the agents of choice for diagnostic imaging and therapy of cancers, because their receptors are overexpressed in various human cancer cells. It has been recently indicated that there is a putative role of NPY in breast tumors. The expression of the two best-inves...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20666
更新日期:2007-01-01 00:00:00
abstract::Interactions between the diketopiperazine cyclo-alanylglycine and four fluorinated alcohols in water-fluoroalcohol mixtures were examined by (1)H[(19)F] intermolecular nuclear Overhauser effects (NOE) experiments. The alcohols studied were trifluoroethanol, hexafluoroacetone trihydrate, 1,1,1,3,3,3-hexafluoroisopropan...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10176
更新日期:2002-08-15 00:00:00
abstract::To examine stabilizing effects of the base pair interaction on a protein scaffold, various peptides with L-α-amino acids bearing a nucleobase in the side chain (nucleobase amino acids; NBAs) were designed based on a G-peptide β-hairpin structure, and their conformational properties were investigated by circular dichro...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21464
更新日期:2010-01-01 00:00:00
abstract::The absorption, CD, and fluorescence emission spectra, and the fluorescence emission and depolarization lifetimes of the human granulocyte-macrophage colony-stimulating factor (hGM-CSF) and related peptides previously tested for their immunological activity, were measured in water at various pHs and temperatures to ob...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360360102
更新日期:1995-07-01 00:00:00
abstract::Surface-enhanced Raman spectroscopy (SERS) in a silver sol assisted by density functional theory (DFT) calculations is shown to be a promising tool in the characterization of platinum complexes and their interaction with nucleic acid bases. This is demonstrated using cisplatin and guanine as a model. The energies and ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10480
更新日期:2003-01-01 00:00:00
abstract::It is becoming increasingly clear that characterization of the protein ensemble-the collection of all conformations of which the protein is capable-will be a critical step in developing a full understanding of the linkage between structure, dynamics, and function. X-ray solution scattering in the small angle (SAXS) an...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21631
更新日期:2011-08-01 00:00:00
abstract::The structures of the helices II-III region and the helix IV region of B. megaterium 5S rRNA have been examined by means of energy minimization and molecular dynamics calculations. Calculated distances between neighboring hydrogen-bonded imino protons in helices II, III, and IV were between 3.5 and 4.5 A. The overall ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360320915
更新日期:1992-09-01 00:00:00
abstract::High-level quantum-chemical and quantum-dynamics calculations are reported on the tautomerization equilibrium and rate constants of guanine and its complexes with one and two water molecules. The results are used to estimate the fraction of guanine present in the cell during DNA synthesis as the unwanted tautomer that...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/1097-0282(2001)61:1<77::AID-BIP10062>3.0.C
更新日期:2001-01-01 00:00:00
abstract::The large abundance of bioactive single- and multiple-stranded cystine-rich peptides in nature has fostered the development of orthogonal thiol-protection schemes and of efficient chemistries for regioselective disulfide formation in synthetic replica for decades. In parallel to these entirely synthetic strategies, an...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.20174
更新日期:2005-01-01 00:00:00
abstract::Amyloid aggregates have been implicated in the pathogenesis of diseases such as type 2 diabetes, Alzheimer's, Parkinson's, and prion disease. Recently determined microcrystal structures of several short peptide segments derived from fibril-forming proteins revealed coexistence of alternative aggregation modes (amyloid...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21731
更新日期:2012-01-01 00:00:00
abstract::Individual-site isotherms for the binding of bacteriophage lambda repressor to the left and right lambda operators have been determined [D. F. Senear, M. Brenowitz, M. A. Shea, and G. K. Ackers (1986) Biochemistry, Vol. 25, pp. 7344-7354.] using the DNAse protection technique [footprinting; D. J. Galas and A. Schmitz ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360330903
更新日期:1993-09-01 00:00:00
abstract::In this paper, we explore the possibility of using experimental observations in the Monte Carlo chain growth method that we have previously developed. In this method, the macromolecule (peptide, protein, nuclei acid, etc.) is grown atom-by-atom (or residue-by-residue, etc.) and partial chains are replicated according ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360331210
更新日期:1993-12-01 00:00:00
abstract::Prolonged exposure (>90 days) of bovine beta-lactoglobulin (BLG) to subdenaturing concentrations of either urea or potassium thiocyanate resulted in the formation of ordered polymers in the form of fibrils. The fibrils obtained with each chaotrope showed major differences in morphology, surface properties, thiol acces...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20704
更新日期:2007-05-01 00:00:00
abstract::For the controlled synthesis of even the simplest dipeptide, the N(alpha)-amino group of one of the amino acids and the C-terminal carboxyl group of the other should both be blocked with suitable protecting groups. Formation of the desired amide bond can now occur upon activation of the free carboxyl group. After coup...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/1097-0282(2000)55:2<123::AID-BIP30>3.0.CO;
更新日期:2000-01-01 00:00:00
abstract::Side-chain lactam bridges linking amino acid residues that are spaced several residues apart in the linear sequence offer a convenient and flexible method for introducing conformational constraints into a peptide structure. The availability of a variety of selectively cleavable protecting groups for amines and carboxy...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.10203
更新日期:2002-01-01 00:00:00
abstract::DNA sequences d-TGAGGAAAGAAGGT (a 14-mer) and d-CTCCTTTCTTCC (a 12-mer) are complementary in parallel orientation forming either Donohue (reverse Watson-Crick) base pairing at neutral pH or Hoogsteen base pairing at slightly acidic pH. The structure of the complex formed by dissolving the two strands in equimolar rati...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0282(199706)41:7<773::AID-BIP6>
更新日期:1997-06-01 00:00:00
abstract::Two-dimensional (2-D) Raman and 2-D IR correlation spectroscopy are applied to analyze changes in the nu(4) region of the IR spectrum and in the nu(1) region of the Raman spectrum during the maturation of hydroxyapatite (HA) following the solution-mediated conversion of amorphous calcium phosphate (ACP) to HA. The nu(...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0282(2000)57:3<129::AID-BIP1>3.
更新日期:2000-01-01 00:00:00
abstract::Core peptide is a hydrophobic peptide derived from the T-cell antigen receptor-alpha chain (TCR-alpha) transmembrane region with therapeutic potential. The mechanism by which the peptide inserts into the membrane, including any requirements to change conformational or association states during the insertion, is unclea...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21423
更新日期:2011-01-01 00:00:00
abstract::RADA-16-I is a self-assembling peptide which forms biocompatible fibrils and hydrogels. We used molecular dynamics simulations, atomic-force microscopy, NMR spectroscopy, and thioflavin T binding assay to examine size, structure, and morphology of RADA-16-I aggregates. We used the native form of RADA-16-I (H-(ArgAlaAs...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22755
更新日期:2016-01-01 00:00:00
abstract::The 13C off-resonance rotating frame spin-lattice relaxation technique is applicable to the study of protein rotational diffusion behavior in a variety of experimental situations. The original formalism of James and co-workers (1978) (J. Amer. Chem. Soc. 100, 3590-3594) was constrained by the assumption of random isot...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360290302
更新日期:1990-02-15 00:00:00
abstract::Cyclotides are multifunctional plant cyclic peptides containing 28-37 amino acid residues and a pattern of three disulfide bridges, forming a motif known as the cyclic cystine knot. Due to their high biotechnological potential, the sequencing and characterization of cyclotide genes are crucial not only for cloning and...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22938
更新日期:2016-11-01 00:00:00
abstract::Purified integral membrane proteins require amphipathic molecules to maintain their solubility in aqueous solutions. These complexes, in turn, are used in studies to characterise the protein structures by a variety of biophysical and structural techniques, including spectroscopy, crystallography, and cryo-electron mic...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.23067
更新日期:2018-08-01 00:00:00
abstract::Structural data produced by a 2-ns molecular dynamics (MD) simulation on Geobacillus alanine racemase (AlaR; PDB: 1SFT) was used to study hydration around the two AlaR active sites. AlaR is a crucial enzyme for bacterial cell wall biosynthesis. It has been shown previously that the potency of an inhibitor can be incre...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20893
更新日期:2008-03-01 00:00:00
abstract::Serine palmitoyltransferase (SPT) catalyses the first step in the de novo biosynthesis of sphingolipids (SLs). It uses a decarboxylative Claisen-like condensation reaction to couple L-serine with palmitoyl-CoA to generate a long-chain base product, 3-ketodihydrosphingosine. SLs are produced by mammals, plants, yeast, ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21482
更新日期:2010-09-01 00:00:00
abstract::DMT1, also known as Nramp2, is an iron transporter, and belongs to the family of Nramp proteins. Disease-causing mutations both in Nramp1 and Nramp2 occurring at the conserved two adjacent glycine residues located within the fourth transmembrane domain (TM4) suggest that TM4 may serve an important biological function....
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20204
更新日期:2005-03-01 00:00:00
abstract::A new series of oxy-peptide nucleic acids (pyrrolidine-based oxy-peptide nucleic acids = POPNAs) of four different stereoisomeric forms (cis-L, cis-D, trans-L, trans-D) have been synthesized. To find a favorable stereoisomer of POPNA for hybridization with DNA or RNA, thermodynamic parameters and conformations of the ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20364
更新日期:2006-01-01 00:00:00
abstract::For mimicking macromolecular crowding of DNA quadruplexes, various crowding agents have been used, typically PEG, with quadruplexes of micromolar strand concentrations. Thermal and thermodynamic stabilities of these quadruplexes increased with the concentration of the agents, the rise depended on the crowder used. A d...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22400
更新日期:2014-04-01 00:00:00