Formation of structured polymers upon controlled denaturation of beta-lactoglobulin with different chaotropes.

abstract：:The total synthesis of a peptide segment corresponding to the DNA-binding segment of Sp1 (positions 532-623) using a native chemical ligation approach is described. The folding of the synthetic segment in the presence of Zn(II) gave a zinc-coordinated protein. The dissociation constant (K(D)) for the DNA binding of th...

journal_title：Biopolymers

authors： Futaki S,Tatsuto K,Shiraishi Y,Sugiura Y

更新日期：2004-01-01 00:00:00

abstract：:In this paper, we explore the possibility of using experimental observations in the Monte Carlo chain growth method that we have previously developed. In this method, the macromolecule (peptide, protein, nuclei acid, etc.) is grown atom-by-atom (or residue-by-residue, etc.) and partial chains are replicated according ...

journal_title：Biopolymers

authors： Bascle J,Garel T,Orland H,Velikson B

更新日期：1993-12-01 00:00:00

abstract：:Tropoelastin is a complex polymeric protein composed primarily of repeating segments of Val-Pro-Gly-Gly, Val-Pro-Gly-Val-Gly, and Ala-Pro-Gly-Val-Gly-Val that occurs in connective tissue and arteries. It has rubber-like extensible properties. A synthetic cyclic dodecapeptide, with a double repeat of the hexapeptide se...

journal_title：Biopolymers

authors： Karle IL,Urry DW

更新日期：2005-03-01 00:00:00

abstract：:A new series of oxy-peptide nucleic acids (pyrrolidine-based oxy-peptide nucleic acids = POPNAs) of four different stereoisomeric forms (cis-L, cis-D, trans-L, trans-D) have been synthesized. To find a favorable stereoisomer of POPNA for hybridization with DNA or RNA, thermodynamic parameters and conformations of the ...

journal_title：Biopolymers

authors： Kitamatsu M,Shigeyasu M,Saitoh M,Sisido M

更新日期：2006-01-01 00:00:00

abstract：:Recently, we isolated and purified a neutral polysaccharide (PGN) from edible fungus Pleurotus geestanus. Its structure was characterized by a range of physical-chemical methods, including high performance anion exchange chromatography, uronic acid, and protein analyses, size exclusion chromatography with ultraviolet,...

journal_title：Biopolymers

authors： Zhang M

更新日期：2010-02-01 00:00:00

abstract：:Designed ligands that inhibit protein-protein interactions involved in gene expression are valuable as reagents for genomics research and as leads for drug discovery efforts. Selective modulation of protein-protein interactions has proven to be a daunting task for synthetic ligands; however, the last decade has seen s...

journal_title：Biopolymers

authors： Guarracino DA,Bullock BN,Arora PS

更新日期：2011-01-01 00:00:00

abstract：:Core peptide is a hydrophobic peptide derived from the T-cell antigen receptor-alpha chain (TCR-alpha) transmembrane region with therapeutic potential. The mechanism by which the peptide inserts into the membrane, including any requirements to change conformational or association states during the insertion, is unclea...

journal_title：Biopolymers

authors： Zheng G,Torres AM,Ali M,Manolios N,Price WS

更新日期：2011-01-01 00:00:00

abstract：:The discovery of extreme halophile microorganisms in the Dead Sea, which are specifically dependent on a multimolar salt environment to survive, stimulated major developments in biology and physical chemistry. The minireview focuses on the molecular level. After a brief introduction to the history of halophile studies...

journal_title：Biopolymers

authors： Zaccai G

更新日期：2013-04-01 00:00:00

abstract：:The preferred helical screw senses of chiral α-amino acids with a C(α)-tetrasubstituted α-carbon atom, as determined in the crystal state by X-ray diffraction analyses on derivatives and peptides, are reviewed. This survey covers C(α)-methylated and C(α)-ethylated α-amino acids, as well as α-amino acids cyclized on th...

journal_title：Biopolymers

authors： Crisma M,Toniolo C

更新日期：2015-01-01 00:00:00

abstract：:The coupling of nuC-O and deltaO-D vibrations in the 1200-900 cm(-1) IR range leads to band shifting in opposite directions, which provides information on intramolecular hydrogen bonding of carbohydrates in aqueous solution. The aqueous solution IR spectra of 2-acetamide-1,6-anhydro-2-deoxy-D-glucopyranose and cis-1,2...

journal_title：Biopolymers

authors： Carmona P,Molina M,Aboitiz N,Vicent C

更新日期：2002-01-01 00:00:00

abstract：:Dynamic and static intensity light scattering techniques were employed to identify conditions allowing preparation of homogeneous solutions of distinct oligomeric states of RecA protein. These hydrodynamically distinguishable oligomer populations of RecA protein were obtained in homogeneous pure quantities sufficient ...

journal_title：Biopolymers

authors： Budzynski DM,Gao X,Benight AS

更新日期：1996-04-01 00:00:00

abstract：:The gel electrophoresis of spherical particles with a radius above 0.2 micron has not been reported yet. In the present study, video phase-contrast light microscopy is used to observe the motion of individual latex spheres, 0.52 micron in radius, during electrophoresis in 0.1% agarose gels. At 2 V/cm, the spheres init...

journal_title：Biopolymers

authors： Griess GA,Serwer P

更新日期：1990-12-01 00:00:00

abstract：:To examine stabilizing effects of the base pair interaction on a protein scaffold, various peptides with L-α-amino acids bearing a nucleobase in the side chain (nucleobase amino acids; NBAs) were designed based on a G-peptide β-hairpin structure, and their conformational properties were investigated by circular dichro...

journal_title：Biopolymers

authors： Uozumi R,Takahashi T,Yamazaki T,Granholm V,Mihara H

更新日期：2010-01-01 00:00:00

abstract：:The aim of this study was to develop a nondestructive method to quantitate relative amounts of n-3 and n-6 polyunsaturated fatty acid (PUFA) species in vegetable oils and oil seeds using Fourier transform IR spectroscopy (FTIR). The alkene Cbond;H stretching vibrations of unsaturated fatty acids in oils showed IR abso...

journal_title：Biopolymers

authors： Yoshida S,Yoshida H

更新日期：2003-12-01 00:00:00

abstract：:The large abundance of bioactive single- and multiple-stranded cystine-rich peptides in nature has fostered the development of orthogonal thiol-protection schemes and of efficient chemistries for regioselective disulfide formation in synthetic replica for decades. In parallel to these entirely synthetic strategies, an...

journal_title：Biopolymers

authors： Moroder L,Musiol HJ,Götz M,Renner C

更新日期：2005-01-01 00:00:00

abstract：:The self-cleaving hepatitis delta virus (HDV) ribozyme is essential for the replication of HDV, a liver disease causing pathogen in humans. The catalytically critical nucleotide C75 of the ribozyme is buttressed by a trefoil turn pivoting around an extruded G76. In all available crystal structures, the conformation of...

journal_title：Biopolymers

authors： Sefcikova J,Krasovska MV,Spacková N,Sponer J,Walter NG

更新日期：2007-04-05 00:00:00

abstract：:The effects of inserting 16 base pair (bp) of alternating CG [(CG)8] near the middle of a much longer restriction fragment (1097 bp) are investigated by measuring various properties that are sensitive to secondary and tertiary structure. Results for this fragment are compared with those for a control fragment (1089 bp...

journal_title：Biopolymers

authors： Kim US,Fujimoto BS,Furlong CE,Sundstrom JA,Humbert R,Teller DC,Schurr JM

更新日期：1993-11-01 00:00:00

abstract：:It has recently been reported that synthetic peptides corresponding to the C-terminal sequence of G alpha, can be used to study the molecular mechanisms of interaction between this protein and G protein coupled receptors (Hamm et al., Science, 1988, Vol. 241, pp. 832-835). A conformational analysis on a 11 amino acids...

journal_title：Biopolymers

authors： Albrizio S,D'Ursi A,Fattorusso C,Galoppini C,Greco G,Mazzoni MR,Novellino E,Rovero P

更新日期：2000-09-01 00:00:00

abstract：:The aim of this study was to investigate the effect of dopa and catecholamines on the generation of oxygen active species during oxidation of farmorubicin by "Fenton-like" reagents using chemiluminescent and spectrophotometric techniques. The tested catechols were found to reduce the light emission accompanying oxidat...

journal_title：Biopolymers

authors： Kruk I,Lichszteld K,Aboul-Enein HY

更新日期：2002-01-01 00:00:00

abstract：:A series of novel nonpeptide inhibitors of the pp60(c-Src) (Src) SH2 domain is described that exploit multifunctional group replacement of the phenylphosphate moiety of phosphotyrosine (pTyr). Relative to an x-ray structure of citrate complexed to the pTyr binding site of the Src SH2 domain, these nonpeptide ligands i...

journal_title：Biopolymers

authors： Sundaramoorthi R,Kawahata N,Yang MG,Shakespeare WC,Metcalf CA 3rd,Wang Y,Merry T,Eyermann CJ,Bohacek RS,Narula S,Dalgarno DC,Sawyer TK

更新日期：2003-01-01 00:00:00

abstract：:A systematic molecular dynamics (MD) simulation has been carried out on collagen-like peptides with different combinations of interruptions in the Gly-X(AA) -Y(AA) repeats. Although experimental studies have been carried out to elucidate the structural consequences of homotrimeric collagen-like peptides, this is the f...

journal_title：Biopolymers

authors： Singam ER,Balamurugan K,Gopalakrishnan R,Subramanian SR,Subramanian V,Ramasami T

更新日期：2012-11-01 00:00:00

abstract：:Elastin-like polypeptide (ELP) fusions have been designed to allow large-scale, nonchromatographic purification of many soluble proteins by using the inverse transition cycling (ITC) method; however, the sensitivity of the aqueous lower critical solubility phase transition temperature (T(t)) of ELPs to the addition of...

journal_title：Biopolymers

authors： Thapa A,Han W,Simons RH,Chilkoti A,Chi EY,López GP

更新日期：2013-01-01 00:00:00

abstract：:The photophysics of Trp-containing peptides is extremely affected by the position of the indole ring with respect to substituents. In this work an unusual temperature fluorescence quenching behavior is presented. The N-tryptophan terminal peptides (N-Trp) show an increase of the static emission intensity as rising the...

journal_title：Biopolymers

authors： Brancaleon L,Crippa PR,Diemmi D

更新日期：1995-12-01 00:00:00

abstract：:Side-chain lactam bridges linking amino acid residues that are spaced several residues apart in the linear sequence offer a convenient and flexible method for introducing conformational constraints into a peptide structure. The availability of a variety of selectively cleavable protecting groups for amines and carboxy...

journal_title：Biopolymers

authors： Taylor JW

更新日期：2002-01-01 00:00:00

abstract：:The general properties of hydroxyethylcellulose (HEC) grafted with 3-glycidoxypropyltrimethoxysilane (GPTMS) or 3-glycidoxypropylmethyldiethoxysilane (GPDMS) were studied for potential biomedical applications. The graft involved a Williamson reaction between the free hydroxyl function of HEC and the epoxy function of ...

journal_title：Biopolymers

authors： Bourges X,Weiss P,Coudreuse A,Daculsi G,Legeay G

更新日期：2002-04-05 00:00:00

abstract：:Peptides represent a rich natural source of potential medicines with one notable pharmaceutical limitation being their relatively short duration of action. A particularly good example of this phenomenon is glucagon-like peptide 1 (GLP), a hormone of appreciable interest for the treatment of type II diabetes. In the na...

journal_title：Biopolymers

authors： De A,DiMarchi RD

更新日期：2010-01-01 00:00:00

abstract：:Actinomycin-D (actD) binds to natural DNA at two different classes of binding sites, weak and strong. The affinity for these sites is highly dependent on DNA sequence and solution conditions, and the interaction appears to be purely entropic driven. Although the entropic character of this reaction has been attributed ...

journal_title：Biopolymers

authors： Ruggiero Neto J,Colombo MF

更新日期：2000-01-01 00:00:00

abstract：:Functional RNAs such as ribosomal RNA and structured domains of mRNA are targets for small molecule ligands that can act as modulators of the RNA biological activity. Natural ligands for RNA display a bewildering structural and chemical complexity that has yet to be matched by synthetic RNA binders. Comparison of natu...

journal_title：Biopolymers

authors： Hermann T

更新日期：2003-09-01 00:00:00

abstract：:Nanoparticle based delivery systems can offer opportunities for targeting, controlled release, and enhanced stability of their drug, protein, or gene therapy payload. This study investigated the use of chitosan in combination with the ionic additives sulfobutyl-ether-7-beta-cyclodextrin (SB-CD) or SB-CD/dextran sulfat...

journal_title：Biopolymers

authors： Chen Y,Siddalingappa B,Chan PH,Benson HA

更新日期：2008-01-01 00:00:00

abstract：:Human hair as alpha-keratin fiber exhibits a complex morphology, which for the context of this investigation is considered as a filament/matrix-composite, comprising the intermediate filaments (IF) and a variety of amorphous protein components as matrix. Differential scanning calorimetry (DSC) under aqueous conditions...

journal_title：Biopolymers

authors： Wortmann FJ,Popescu C,Sendelbach G

更新日期：2006-12-15 00:00:00