Conformational studies on a synthetic C-terminal fragment of the alpha subunit of G(S) proteins.

abstract：:Antagonism of the calcitonin gene-related peptide (CGRP) receptor may be a useful approach for migraine treatment. Selective PEGylated peptide antagonists to the CGRP receptor are described, derived from CGRP(8-37) with polymer derivatization at an engineered lysine-25 residue. Potent PEGylated peptides with improved ...

journal_title：Biopolymers

authors： Miranda LP,Shi L,Holder JR,Wright M,Gegg CV,Johnson E,Wild K,Stenkilsson M,Doellgast G,Manning BH,Salyers K

更新日期：2013-07-01 00:00:00

abstract：:In this study, we identified and chemically synthesized three cationic and amphipathic peptides (Glycinin-17, BCAS-16, and BCBS-11) from soybean proteins. These peptides had high isoelectric points, high positive net charges, and included multiple hydrophobic amino acids. Subsequently, we identified multiple functions...

journal_title：Biopolymers

authors： Taniguchi M,Saito K,Nomoto T,Namae T,Ochiai A,Saitoh E,Tanaka T

更新日期：2017-07-01 00:00:00

abstract：:A novel nucleic acid model using peptide ribonucleic acid (PRNA), which contains 5'-amino-5'-deoxypyrimidine ribonucleoside as a recognition site for nucleic acids, has been designed, synthesized and applied to the external reversible control of recognition behavior of the complementary polynucleotide through the orie...

journal_title：Biopolymers

authors： Wada T,Sato H,Inoue Y

更新日期：2004-01-01 00:00:00

abstract：:For the controlled synthesis of even the simplest dipeptide, the N(alpha)-amino group of one of the amino acids and the C-terminal carboxyl group of the other should both be blocked with suitable protecting groups. Formation of the desired amide bond can now occur upon activation of the free carboxyl group. After coup...

journal_title：Biopolymers

authors： Albericio F

更新日期：2000-01-01 00:00:00

abstract：:The synthetic peptide Gly-L-Ala-L-Phe (C14H19N3O4.2H2O; GAF) crystallizes in the monoclinic space group P2I1), with a = 5.879(1), b = 7.966(1), c = 17.754(2) A, beta = 95.14(2) degrees, Dx = 1.321 g cm-3, and Z = 2. The crystal structure was solved by direct methods using the program SHELXS-86 and refined to an R valu...

journal_title：Biopolymers

authors： Ramasubbu N,Parthasarathy R

更新日期：1989-07-01 00:00:00

abstract：:The interaction of natural calf thymus DNA with Cr(3+) ions was studied at room temperature by means of vibrational CD (VCD) and infrared absorption (ir) spectroscopy, and atomic force microscopy (AFM). Cr(3+) ion binding mainly to N(7) (G) and to phosphate groups was demonstrated. Psi-type VCD spectra resembling elec...

journal_title：Biopolymers

authors： Andrushchenko V,Leonenko Z,Cramb D,van de Sande H,Wieser H

更新日期：2001-01-01 00:00:00

abstract：:Peptide recognition by G-protein coupled receptors (GPCRs) is reviewed with an emphasis on the indirect approach used to determine the receptor-bound conformation of peptide ligands. This approach was developed in response to the lack of detailed structural information available for these receptors. Recent advances in...

journal_title：Biopolymers

authors： Marshall GR

更新日期：2001-01-01 00:00:00

abstract：:It was shown earlier, from database analysis, model building studies, and molecular dynamics simulations that formation of cross-strand bifurcated or Extra Watson-Crick hydrogen (EWC) bonds between successive base pairs may lead to extra rigidity to DNA double helices of certain sequences. The strengths of these hydro...

journal_title：Biopolymers

authors： Bandyopadhyay D,Bhattacharyya D

更新日期：2006-10-15 00:00:00

abstract：:Raman spectra of series of aqueous solutions of peptides containing two amino acids, glycine-X, alanine-X, and serine-X, where X is an uncharged amino acid, show that the amide III band shifts systematically to lower frequencies as the side chain of the X amino acid becomes larger. The range of this shift is about 20 ...

journal_title：Biopolymers

authors： Weaver JL,Williams RW

更新日期：1990-01-01 00:00:00

abstract：:Different hexameric forms of insulin have been crystallized from a variety of conditions. In the presence of 1% phenol, 1.0 M sodium chloride, and at a pH of 8.5, a rhombohedral form is produced with two monomers in the asymmetric unit, space group R3, a = 79.92 A and c = 40.39 A. The structure has been solved and ref...

journal_title：Biopolymers

authors： Smith GD,Dodson GG

更新日期：1992-04-01 00:00:00

abstract：:Depsipeptide analogues of peptide sequences can help in elucidating the role of specific hydrogen bonds in determining the conformation in peptides. The repeating pentapeptide and hexapeptide sequences of elastin have been suggested to contain a type II beta-turn with a 4----1 hydrogen bond. Depsipeptide analogues of ...

journal_title：Biopolymers

authors： Arad O,Goodman M

更新日期：1990-10-01 00:00:00

abstract：:The structures of the helices II-III region and the helix IV region of B. megaterium 5S rRNA have been examined by means of energy minimization and molecular dynamics calculations. Calculated distances between neighboring hydrogen-bonded imino protons in helices II, III, and IV were between 3.5 and 4.5 A. The overall ...

journal_title：Biopolymers

authors： Kim JH,Marshall AG

更新日期：1992-09-01 00:00:00

abstract：:The IR data for the R1 CO-O-CHR2 -CO-NHR3 derivatives are interpreted in terms of a H…π interaction involving the NH bond and the π orbitals of the ester function and giving rise to a high ν(CO) frequency and a low ν frequency. The resulting molecular conformation corresponds to the angular values ϕ # -90°, ψ # 0°. ...

journal_title：Biopolymers

authors： Boussard G,Marraud M

更新日期：1981-01-01 00:00:00

abstract：:This report details the observation of (a) the intact double helix of DNA at 0% relative humidity in poly(dA).poly(dT) and poly(dA-dT).poly(dA-dT) films, (b) the fractal-like growth of DNA crystals in films of poly(dA).poly(dT), and (c) poly(dA).poly(dT) with adenines in the B form and thymines in the A form. Observat...

journal_title：Biopolymers

authors： Sclavi B,Peticolas WL,Powell JW

更新日期：1994-08-01 00:00:00

abstract：:We have extended our studies of Trp/Trp to other Aryl/Aryl through-space interactions that stabilize hairpins and other small polypeptide folds. Herein we detail the NMR and CD spectroscopic features of these types of interactions. NMR data remains the best diagnostic for characterizing the common T-shape orientation....

journal_title：Biopolymers

authors： Anderson JM,Kier BL,Jurban B,Byrne A,Shu I,Eidenschink LA,Shcherbakov AA,Hudson M,Fesinmeyer RM,Andersen NH

更新日期：2016-06-01 00:00:00

abstract：:RNA and DNA molecules experience motions on a wide range of time scales, ranging from rapid localized motions to much slower collective motions of entire helical domains. The many functions of RNA in biology very often require this molecule to change its conformation in response to biological signals in the form of sm...

journal_title：Biopolymers

authors： Shajani Z,Varani G

更新日期：2007-08-05 00:00:00

abstract：:The phase content of amylose-water mixtures (0.7/0.3 w/w) has been analyzed by transmission Fourier transform infrared (FTIR) spectroscopy in the 1175-950 cm(-1) region. Spectra are obtained under three different conditions: (a) as a function of temperature (T) from 25 to 97 degrees C; (b) at room temperature (RT), af...

journal_title：Biopolymers

authors： Bernazzani P,Chapados C,Delmas G

更新日期：2001-03-01 00:00:00

abstract：:Shorter analogues of a continuous epitope of hepatitis A virus, VP3(110-121) peptide, failed to react with convalescent sera, indicating the importance of the entire peptide in the epitope structure. To better understand the influence of the structural properties of this 12-mer peptide epitope on its biological activi...

journal_title：Biopolymers

authors： Sospedra P,Muñoz M,García M,Alsina MA,Mestres C,Haro I

更新日期：2000-12-01 00:00:00

abstract：:Infrared spectra of the amino acid residues in H2O solution have been obtained in the 1800-1400-cm-1 region. It has been established that amino acid residues of arginine, asparagine, glutamine, aspartic and glutamic acids, lysine, tyrosine, histidine, and phenylalanine have intensive absorption in this spectral region...

journal_title：Biopolymers

authors： Venyaminov SYu,Kalnin NN

更新日期：1990-01-01 00:00:00

abstract：:Individual ethidium-stained DNA molecules, embedded in an agarose gel made with electrophoresis buffer (0.05 molar salt), are observed using a fluorescence microscope. In the first experiment, open circular 66 kilobase pair (kbp) plasmids, immobilized by agarose fibers threaded through their centers, display entropic ...

journal_title：Biopolymers

authors： Smith SB,Bendich AJ

更新日期：1990-07-01 00:00:00

abstract：:We studied fluorescence enhancements of fluorescein tethered to silver colloids of different size. Thiolated 23-mer oligonucleotide (ss DNA-SH) was bound selectively to silver colloids deposited on 3-aminopropyltriethoxysilane (APS)-treated quartz slides. Fluorescein-labeled complementary oligonucleotide (ss Fl-DNA) w...

journal_title：Biopolymers

authors： Lukomska J,Malicka J,Gryczynski I,Leonenko Z,Lakowicz JR

更新日期：2005-01-01 00:00:00

abstract：:Spontaneous cortical activity of single neurons is often either dismissed as noise, or is regarded as carrying no functional significance and hence is ignored. Our findings suggest that such concepts should be revised. We explored the coherent population activity of neuronal assemblies in primary sensory area in the a...

journal_title：Biopolymers

authors： Grinvald A,Arieli A,Tsodyks M,Kenet T

更新日期：2003-03-01 00:00:00

abstract：:While our understanding of the folding and structure of water-soluble proteins has progressed to the point where they can be artificially designed and produced from first principles, there has been only limited work toward the de novo design of membrane proteins. Such studies have been hindered in large part due to th...

journal_title：Biopolymers

authors： Tulumello DV,Johnson RM,Isupov I,Deber CM

更新日期：2012-01-01 00:00:00

abstract：:We have previously proposed a model for the delta-opioid receptor binding conformation of the high affinity tetrapeptide Tyr-c[D-Cys-Phe-D-Pen]OH (JOM-13) based on experimental and theoretical conformational analysis of this peptide and a correlation of conformational preferences of further conformationally restricted...

journal_title：Biopolymers

authors： Mosberg HI,Dua RK,Pogozheva ID,Lomize AL

更新日期：1996-09-01 00:00:00

abstract：:Small-angle x-ray scattering was used for studying intermediate species, isolated after heat-induced aggregation of the A variant of bovine beta-lactoglobulin. The intermediates were separated in two fractions, the heated metastable dimer and heated metastable oligomers larger than the dimer. The pair distance distrib...

journal_title：Biopolymers

authors： Carrotta R,Arleth L,Pedersen JS,Bauer R

更新日期：2003-10-01 00:00:00

abstract：:Although many anticancer drugs exert their biological activity by forming DNA interstrand crosslinks (ICLs), the thermodynamics of biologically relevant long crosslinked DNAs has not been intensively studied in contrast to short duplexes. Here, we carry out computer modeling of the shift of melting temperature of long...

journal_title：Biopolymers

authors： Chang CL,Lando DY,Fridman AS,Hu CK

更新日期：2012-10-01 00:00:00

abstract：:From Auricularia auricula-judae, a water soluble beta-D-glucan, named as AAG, was isolated by extraction with 70% ethanol/water solution. Its chemical structure was analyzed by gas chromatography (GC), gas chromatography-mass spectrometry (GC-MS), matrix-assisted laser desorption /ionization (MALDI)-time of flight (TO...

journal_title：Biopolymers

authors： Ma Z,Wang J,Zhang L

更新日期：2008-07-01 00:00:00

abstract：:Differential scanning calorimetry measurements performed on RNase A in aqueous binary solutions containing different concentrations of urea, tetramethylurea, guanidinium chloride, and guanidinium thiocyanate, and in aqueous ternary solutions, containing the same denaturants plus 1 M trimethylamine N-oxide, TMAO, demon...

journal_title：Biopolymers

authors： Vigorita M,Cozzolino S,Oliva R,Graziano G,Del Vecchio P

更新日期：2018-08-01 00:00:00

abstract：:The conformation of a 13-residue C-peptide analogue of ribonuclease A, suc-AET-AAAKFLRAHA-CONH2, in NaDodSO4 solutions with respect to temperature was studied with CD. The equilibrium constant of unfolding yielded a straight line in a van't Hoff plot. In 10 mM NaDodSO4, delta G mu = 120 cal/mol, delta H mu = 700 cal/m...

journal_title：Biopolymers

authors： Wu CS,Yang JT

更新日期：1990-01-01 00:00:00

abstract：:The kinetics of the hydrodynamic volume change associated with the unfolding and refolding of a globular protein can be observed using high performance size exclusion chromatography. Chromatographic profiles that evidence such dynamics can be simulated using equations in which chromatographic partitioning and the conf...

journal_title：Biopolymers

authors： Shalongo W,Jagannadham M,Stellwagen E

更新日期：1993-01-01 00:00:00