Abstract:
:Differential scanning calorimetry measurements performed on RNase A in aqueous binary solutions containing different concentrations of urea, tetramethylurea, guanidinium chloride, and guanidinium thiocyanate, and in aqueous ternary solutions, containing the same denaturants plus 1 M trimethylamine N-oxide, TMAO, demonstrate that the latter has a general counteracting ability at pH 7.0, but not at pH 4.0. Experimental data rule out the idea that counteraction originates from direct interactions between TMAO molecules and denaturing agents. A rationalization is provided on the basis of a theoretical approach grounded on the solvent-excluded volume effect, whose magnitude depends on the density of aqueous solutions.
journal_name
Biopolymersjournal_title
Biopolymersauthors
Vigorita M,Cozzolino S,Oliva R,Graziano G,Del Vecchio Pdoi
10.1002/bip.23104subject
Has Abstractpub_date
2018-08-01 00:00:00pages
e23104issue
10eissn
0006-3525issn
1097-0282journal_volume
109pub_type
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