Abstract:
:Raman spectra of normal hair shafts and hair shafts from patients exhibiting trichothiodystrophy (TTD) were obtained using line focus laser illumination. Because hair from TTD patients has a significant decrease in the content of the sulfur-containing amino acids in comparison to normal hair, the 550-500 cm(-1) disulfide stretching mode region of the Raman spectrum was examined in detail. A quantitative spectral analysis demonstrates significant increases in the two energetically less favored gauche-gauche-trans (g-g-t) and trans-gauche-trans (t-g-t) forms. These observations suggest that the increased amounts of these less stable disulfide conformers are contributing factors to or associated with the hair brittleness observed for this congenital disorder. Structure-spectra correlations for the three dominant disulfide conformers are confirmed by quantum chemical calculations using modern density functional theory (DFT).
journal_name
Biopolymersjournal_title
Biopolymersauthors
Schlücker S,Liang C,Strehle KR,DiGiovanna JJ,Kraemer KH,Levin IWdoi
10.1002/bip.20515subject
Has Abstractpub_date
2006-08-15 00:00:00pages
615-22issue
6eissn
0006-3525issn
1097-0282journal_volume
82pub_type
杂志文章相关文献
BIOPOLYMERS文献大全abstract::In a seminal paper, Pakula and Sauer (Nature, 1990, 344, 363-364) demonstrated that the increase in side-chain hydrophobicity has a reverse relationship with protein stability. We have addressed this problem with several examples of mutants that span at different locations in protein structure based on secondary struc...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21187
更新日期:2009-07-01 00:00:00
abstract::Ultraviolet hyperchromicity experiments indicate that in DNA duplex formation, a C-T mismatch is destabilizing in the center of a duplex, but behaves as a stable base pair at the terminus of a duplex. The C-T base pair is thought to contain two hydrogen bonds, but has thermodynamic parameters (delta Ho and delta Go of...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360310908
更新日期:1991-08-01 00:00:00
abstract::Useful methods of protein labeling via functional peptide tags have been developed in the field of proteome and chemical biology. New tag-probe pairs based on leucine zipper peptides for labeling target proteins are described. These consist of an α-helical probe peptide with an environmental-sensitive fluorescent dye ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21444
更新日期:2010-01-01 00:00:00
abstract::Spin-spin and spin-lattice 1H-nmr relaxation times of the sea anemone polypeptide anthopleurin-A were measured at frequencies of 200, 300, 400, and 500 MHz. Relaxation times were fitted iteratively by least squares regression to the isotropic tumbling model, Woessner's model for anisotropic motion, and Lipari and Szab...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360280303
更新日期:1989-03-01 00:00:00
abstract::We describe the synthesis of several polyamides that retain the secondary structure of proteins and contain derivatizable side chains. The derivatizable side chain allows for further reaction of the polymer chain (e.g., chain cross-linking or addition of pendant groups). Polymers of alpha-amino acids containing a term...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360350509
更新日期:1995-05-01 00:00:00
abstract::In this article, the pulsed double electron-electron resonance in electron spin-echo (PELDOR) technique is applied to study the self-aggregation of spin-labeled zervamicin IIA, a hexadecapeptide antibiotic of fungal origin, which is known to form ion channels in a phospholipid double layer. Measurements of the ion cha...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10208
更新日期:2002-09-01 00:00:00
abstract::The design of modified nucleic acid aptamers is improved by considering thermodynamics and kinetics of their association/dissociation processes. Locked Nucleic Acids (LNA) is a promising class of nucleic acid analogs. In this work the thermodynamic and kinetic properties of a LNA quadruplex formed by the TGGGT sequenc...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20591
更新日期:2006-12-15 00:00:00
abstract::Molecular modeling is used to demonstrate that the major structural deformations of DNA caused by four different minor groove binding proteins, TBP, SRY, LEF-1, and PurR, can all be mimicked by stretching the double helix between two 3'-phosphate groups flanking the binding region. This deformation reproduces the wide...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0282(19990415)49:5<341::AID-BIP
更新日期:1999-04-15 00:00:00
abstract::The HPLC retention behavior of three complete single methionine and methionine sulfoxide replacement sets of two 18-mer model peptides and neuropeptide Y (NPY) were investigated. All peptides were prepared by multiple solid-phase peptide synthesis. Plotting the retention time differences between methionine and methion...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0282(199608)39:2%3C207::AID-BIP
更新日期:1996-08-01 00:00:00
abstract::In N-methyl amino acids, the hydrogen of the N-H group is replaced with a bulky methyl group. While this change is expected to destabilize helical structures, the amount of destabilization is not known. Here the N-methyl group is placed into several positions of the helical peptides, acetyl-WGG(EAAAR)4A-amide and acet...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0282(1996)40:6%3C609::AID-BIP2%
更新日期:1996-01-01 00:00:00
abstract::Lung cancer is usually fatal once it becomes metastatic. However, in order to develop metastases, a tumor usually invades the basal membrane and enters the vascular or lymphatic system. In this study, a three-dimensional artificial membrane using collagen type I, one of the main components of basal membranes, was esta...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20297
更新日期:2005-08-15 00:00:00
abstract::Examining solute-induced changes in protein conformational equilibria is a long-standing method for probing the role of water in maintaining protein stability. Interpreting the molecular details governing the solute-induced effects, however, remains controversial. We present experimental and theoretical data for osmol...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0282(20000405)53:4<293::AID-BIP
更新日期:2000-04-05 00:00:00
abstract::Antimicrobial peptides (AMPs) have been the focus of intense research towards the finding of a viable alternative to current small-molecule antibiotics, owing to their commonly observed and naturally occurring resistance against pathogens. However, natural peptides have many problems such as low bioavailability and hi...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22885
更新日期:2016-09-01 00:00:00
abstract::We have previously proposed a model for the delta-opioid receptor binding conformation of the high affinity tetrapeptide Tyr-c[D-Cys-Phe-D-Pen]OH (JOM-13) based on experimental and theoretical conformational analysis of this peptide and a correlation of conformational preferences of further conformationally restricted...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(sici)1097-0282(199609)39:3<287::aid-bip2>
更新日期:1996-09-01 00:00:00
abstract::Cyclotides are disulfide-rich miniproteins with a circular backbone and a knotted arrangement ofdisulfide bonds. Because these plant-derived peptides are resistant to degradation and exhibit a diverse range of bioactivity they have become important agronomic and industrial objectives. They belong to a group of compoun...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.21466
更新日期:2010-01-01 00:00:00
abstract::We have determined using NMR the structure of the complex between the third double-stranded RNA-binding domain (dsRBD3) of Drosophila Staufen protein and a RNA stem-loop with optimal binding properties in vitro. This work was designed to understand how dsRBD proteins bind RNA and to investigate the role of Staufen dsR...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/1097-0282(1999)52:4<181::AID-BIP1003>3.0.C
更新日期:1999-01-01 00:00:00
abstract::The DNA-binding protein HU from Bacillus stearothermophilus (HUBst) is a dimer with a molecular weight of 195 kDa that is capable of bending DNA. An x-ray structure has been determined previously [Tanaka et al. 1984) Nature, vol. 310, pp. 376-381], but no structure could be established for a large part of the supposed...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(sici)1097-0282(1996)40:5<553::aid-bip13>3
更新日期:1996-01-01 00:00:00
abstract::The maltose transporter from Escherichia coli is one of the ATP-binding cassette (ABC) transporters that utilize the energy from ATP hydrolysis to translocate substrates across cellular membranes. Until 2011, three crystal structures have been determined for maltose transporter at different states in the process of tr...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22455
更新日期:2014-07-01 00:00:00
abstract::The coupling of nuC-O and deltaO-D vibrations in the 1200-900 cm(-1) IR range leads to band shifting in opposite directions, which provides information on intramolecular hydrogen bonding of carbohydrates in aqueous solution. The aqueous solution IR spectra of 2-acetamide-1,6-anhydro-2-deoxy-D-glucopyranose and cis-1,2...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10024
更新日期:2002-01-01 00:00:00
abstract::The absorption, CD, and fluorescence emission spectra, and the fluorescence emission and depolarization lifetimes of the human granulocyte-macrophage colony-stimulating factor (hGM-CSF) and related peptides previously tested for their immunological activity, were measured in water at various pHs and temperatures to ob...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360360102
更新日期:1995-07-01 00:00:00
abstract::Human saliva contains hundreds of small proline-rich peptides originated by the proteolytic cleavage of the salivary basic Proline-Rich Proteins. Nevertheless only for few of them a specific biological activity has been assigned to date. Among them, the 1932 Da peptide (p1932) has been patented as an anti-HIV agent. I...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22889
更新日期:2016-09-01 00:00:00
abstract::Study of model beta-hairpin peptides allows for better understanding of the factors involved in the formation of beta-sheet secondary structure in proteins. It is known that turn sequence, sidechain-sidechain interactions, interstrand hydrogen bonding, and beta-sheet propensity of residues are all important for beta-h...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20840
更新日期:2008-01-01 00:00:00
abstract::Solid-state nmr spectroscopy provides a robust method for investigating polypeptides that have been prepared by chemical synthesis and that are immobilized by strong interactions with solid surfaces or large macroscopic complexes. Solid-state nmr spectroscopy has been widely used to investigate membrane polypeptides o...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0282(1999)51:3<174::AID-BIP2>3.
更新日期:1999-01-01 00:00:00
abstract::The synthetic peptide of sequence H-Ala-Ser-Thr-Thr-Thr-Asn-Tyr-Thr-OH, termed peptide T, a competitor of the Human Immunodeficiency Virus in the binding to human T cells, and its C-terminal pentapeptide fragment, were studied by 1H-nmr in DMSO solution to determine conformational preferences. The observation of nucle...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360280142
更新日期:1989-01-01 00:00:00
abstract::Bleomycins A(2) and B(2) are the two active components in the antineoplastic drug Blenoxane. DNA is targeted by this drug in cancer cells and the mode of action of this drug involves DNA binding. Ambiguity exists as to the way in which bleomycin binds to DNA. Raman spectroscopy was used to examine both calf thymus DNA...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/1097-0282(1999)52:3<110::AID-BIP20>3.0.CO;
更新日期:1999-01-01 00:00:00
abstract::Solid state nmr methods were applied to the study of the motions and structural heterogeneity in polyglycine, sodium poly(L-glutamate), and poly(L-alanine). The response of both the main-chain and side-chain resonances to the addition of water was studied using static and magic-angle sample-spinning line shapes as wel...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360300705
更新日期:1990-01-01 00:00:00
abstract::We developed dictionaries of two-, three-, and five-residue patterns in proteins and computed the average solvent accessibility of the central residues in their native proteins. These dictionaries serve as a look-up table for making subsequent predictions of solvent accessibility of amino acid residues. We find that p...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20113
更新日期:2004-10-15 00:00:00
abstract::The comprehensive understanding of disassembly mechanism of amyloid fibrils requires nano-scale characterization of the mechanical properties of amyloid fibrils during the disassembly process. In this work, gemini surfactant C12 C6 C12 Br2 micelles were used as a probe to disassemble Aβ(1-40) fibrils. The microstructu...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22992
更新日期:2017-02-01 00:00:00
abstract::Protein flexibility and conformational diversity is well known to be a key characteristic of the function of many proteins. Human blood coagulation proteins have multiple substrates, and various protein-protein interactions are required for the smooth functioning of the coagulation cascade to maintain blood hemostasis...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21062
更新日期:2008-12-01 00:00:00
abstract::Guanine-rich sequences are able to form quadruplexes consisting of G-quartet structural units. Quadruplexes play an important role in the regulation of gene expression and have therapeutic and biotechnological potential. The HIV-1 integrase inhibitor, (GGGT)4 , and its variants demonstrate unusually high thermal stabi...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22916
更新日期:2016-11-01 00:00:00