Energetic aspects of locked nucleic acids quadruplex association and dissociation.

abstract：:The present study characterizes the unfolding and folding processes of recombinant manganese peroxidase. This enzyme contains five disulfide bonds, two calcium ions, and one heme prosthetic group. Circular dichroism in the far UV was used to monitor global changes of the protein secondary structure, whereas UV-visible...

journal_title：Biopolymers

authors： Banci L,Bartalesi I,Ciofi-Baffoni S,Tien M

更新日期：2003-01-01 00:00:00

abstract：:Individual ethidium-stained DNA molecules, embedded in an agarose gel made with electrophoresis buffer (0.05 molar salt), are observed using a fluorescence microscope. In the first experiment, open circular 66 kilobase pair (kbp) plasmids, immobilized by agarose fibers threaded through their centers, display entropic ...

journal_title：Biopolymers

authors： Smith SB,Bendich AJ

更新日期：1990-07-01 00:00:00

abstract：:The DNA-binding protein HU from Bacillus stearothermophilus (HUBst) is a dimer with a molecular weight of 195 kDa that is capable of bending DNA. An x-ray structure has been determined previously [Tanaka et al. 1984) Nature, vol. 310, pp. 376-381], but no structure could be established for a large part of the supposed...

journal_title：Biopolymers

authors： Boelens R,Vis H,Vorgias CE,Wilson KS,Kaptein R

更新日期：1996-01-01 00:00:00

abstract：:An improved model for calculating the CD of polypeptides has been developed. Excited state wavefunctions were derived from CNDO/S (complete neglect of differential overlap, spectroscopic) calculations on N-methylacetamide. Four discrete peptide-localized transitions were employed: pi 0 pi* (NV1), pi* + pi* (NV2), n pi...

journal_title：Biopolymers

authors： Manning MC,Woody RW

更新日期：1991-04-01 00:00:00

abstract：:The IgY antibodies offer an attractive alternative to mammalian IgGs in research, diagnosis and medicine. The isolation of immunoglobulin Y from the egg yolks is efficient and economical, causing minimal suffering to animals. Here we present the methodology for the production of IgY antibodies specific to Staphylococc...

journal_title：Biopolymers

authors： Walczak M,Grzywa R,Łupicka-Słowik A,Skoreński M,Bobrek K,Nowak D,Boivin S,Brown EL,Oleksyszyn J,Sieńczyk M

更新日期：2015-09-01 00:00:00

abstract：:Using internal conformational degrees of freedom for biopolymers as natural variables, and introducing a Lagrangian dynamics approach, one can simulate time-dependent processes over a much longer time scale than in classical Newtonian molecular dynamics (MD) techniques. Two factors contribute to this: a substantial re...

journal_title：Biopolymers

authors： Rudnicki WR,Lesyng B,Harvey SC

更新日期：1994-03-01 00:00:00

abstract：:Fluorescein-labeled antibodies are widely used in clinical assays and fluorescence microscopy. The fluorescent signal per labeled antibody is limited by fluorescein self-quenching, which occurs when the antibody is heavily labeled with multiple fluoresceins. We examined immunoglobulin G (IgG) when labeled with 0.7 to ...

journal_title：Biopolymers

authors： Lakowicz JR,Malicka J,Huang J,Gryczynski Z,Gryczynski I

更新日期：2004-08-15 00:00:00

abstract：:The comprehensive structural analysis reported herein of eight N-glycopeptides, in three different solvents, is based on quantitative CD experiments, homonuclear nuclear Overhauser effect measurements, and molecular dynamics (MD) calculations. Although several orientations of the two amide planes attached to the carbo...

journal_title：Biopolymers

authors： Perczel A,Kollát E,Hollósi M,Fasman GD

更新日期：1993-04-01 00:00:00

abstract：:The dissociation and assembly of quadruplex DNA structures (and a few quadruplex RNAs) have been characterized at several levels of rigor, ranging from gross descriptions of factors that govern each process, to semiquantitative comparisons of the relative abilities of these factors to induce stabilization or destabili...

journal_title：Biopolymers

authors： Hardin CC,Perry AG,White K

更新日期：2000-01-01 00:00:00

abstract：:The occurrence of a highly conserved 11-mer repeating motif in the primary sequence is a major characteristic of group 3 late embryogenesis abundant (LEA3) proteins, which are strongly associated with abiotic stress tolerance of the plants. In this study, the three-dimensional structure, mimetic membrane association, ...

journal_title：Biopolymers

authors： Xue R,Liu Y,Zheng Y,Wu Y,Li X,Pei F,Ni J

更新日期：2012-01-01 00:00:00

abstract：:A topochemical model to explain the biological activity of dendrotoxin-I (DTX-I), a potent blocker for potassium channels, was developed by searching common spatial arrangements of functionally important residues between DTX-I, alpha-dendrotoxin, dendrotoxin-K, BgK, ShK, and charybdotoxin. The first three are structur...

journal_title：Biopolymers

authors： Katoh E,Nishio H,Inui T,Nishiuchi Y,Kimura T,Sakakibara S,Yamazaki T

更新日期：2000-07-01 00:00:00

abstract：:Molecular structures for parallel DNA and RNA double helices with Hoogsteen pairing are proposed for the first time. The DNA helices have sugars in the C2'-endo region and the phosphodiester conformations are (trans, gauche-), and the RNA helices have sugars in the C3'-endo region and the phosphodiester conformations ...

journal_title：Biopolymers

authors： Raghunathan G,Miles HT,Sasisekharan V

更新日期：1994-12-01 00:00:00

abstract：:Collagen is a natural protein, which is used as a vital biomaterial in tissue engineering. The major concern about native collagen is lack of its thermal stability and weak resistance to proteolytic degradation. In this scenario, the crosslinking compounds used for stabilization of collagen are mostly of chemical natu...

journal_title：Biopolymers

authors： Usharani N,Jayakumar GC,Kanth SV,Rao JR

更新日期：2014-08-01 00:00:00

abstract：:The rheological characteristics of gastric and duodenal mucin solutions, the building blocks of the mucus layer that covers the epithelia of the two organs, were investigated using particle tracking microrheology. We used biochemically well characterized purified porcine mucins (MUC5AC and MUC2) as models for human mu...

journal_title：Biopolymers

authors： Georgiades P,Pudney PD,Thornton DJ,Waigh TA

更新日期：2014-04-01 00:00:00

abstract：:Late expression factor 4 (LEF4) is one of the four subunits of Autographa californica nuclear polyhedrosis virus (AcNPV) RNA polymerase. LEF4 was overexpressed in Escherichia coli and recombinant protein was subjected to structural characterization. Chemical induced unfolding of LEF4 was investigated using intrinsic f...

journal_title：Biopolymers

authors： Rasheedi S,Suragani M,Haq SK,Ghosh S,Ehtesham NZ,Hasnain SE

更新日期：2009-07-01 00:00:00

abstract：:Multiple, independent sites or domains behave, on chemical change, in a manner predicted by Langmuir. Distortions of this behavior have been attributed to interactions between the domains, which vary with the progress of the changes occurring at the sites or domains. The two main models for nearest neighbor interactio...

journal_title：Biopolymers

authors： Saroff HA,Kiefer JE

更新日期：1999-05-01 00:00:00

abstract：:A new series of oxy-peptide nucleic acids (pyrrolidine-based oxy-peptide nucleic acids = POPNAs) of four different stereoisomeric forms (cis-L, cis-D, trans-L, trans-D) have been synthesized. To find a favorable stereoisomer of POPNA for hybridization with DNA or RNA, thermodynamic parameters and conformations of the ...

journal_title：Biopolymers

authors： Kitamatsu M,Shigeyasu M,Saitoh M,Sisido M

更新日期：2006-01-01 00:00:00

abstract：:The enhanced nucleophilicity and redox sensitivity of the thiol group renders cysteine residues susceptible to numerous electrophilic and oxidative post-translational modifications to form disulfides, sulfenic acids, nitrosothiols, and lipid-modified species. Outside of these well-characterized modifications of cystei...

journal_title：Biopolymers

authors： Shannon DA,Weerapana E

更新日期：2014-02-01 00:00:00

abstract：:The quantum yield for cyclobutyl-pyrimidine dimerization in DNA has been observed to increase approximately linearly with increasing pyrimidine tract length. A model without adjustable parameters for this yield is proposed based on energy delocalization, vibronic symmetry switching, and saturation statistics that desc...

journal_title：Biopolymers

authors： Texter J

更新日期：1992-01-01 00:00:00

abstract：:The indirect solvent-induced effect on the free energy of binding of biopolymers is examined within the framework of classical statistical mechanics. We focus specifically on the role of the solute-solvent hydrogen bonding. In particular, we have estimated the first order solvent effect on the indirect interaction bet...

journal_title：Biopolymers

authors： Ben-Naim A,Ting KL,Jernigan RL

更新日期：1990-05-01 00:00:00

abstract：:Simulated ir absorption and vibrational CD (VCD) spectra of four alanine-based octapeptides, each having its main chain constrained to a different secondary structure conformation, were analyzed and compared with experimental results for several different peptides. The octapeptide simulations were based on transfer of...

journal_title：Biopolymers

authors： Bour P,Kubelka J,Keiderling TA

更新日期：2000-04-15 00:00:00

abstract：:Seven water-insoluble (1 --> 3)-beta-D-glucan fractions TM8-1 to TM8-7 with weight-average molecular mass M(w) ranged from 2.22 to 77.4 x 10(4) obtained from the sclerotia of Pleurotus tuber-regium were carboxymethylated to produce the water-soluble fractions CTM8-1 to CTM8-7 with M(w) ranged from 3.87 to 87.8 x 10(4)...

journal_title：Biopolymers

authors： Zhang M,Zhang L,Cheung PC

更新日期：2003-02-01 00:00:00

abstract：:Ultraviolet hyperchromicity experiments indicate that in DNA duplex formation, a C-T mismatch is destabilizing in the center of a duplex, but behaves as a stable base pair at the terminus of a duplex. The C-T base pair is thought to contain two hydrogen bonds, but has thermodynamic parameters (delta Ho and delta Go of...

journal_title：Biopolymers

authors： Vanhommerig SA,van Genderen MH,Buck HM

更新日期：1991-08-01 00:00:00

abstract：:Since the advent of single particle/molecule microscopies, researchers have applied these techniques to understanding the fluid membranes of cells. By observing diffusion of membrane proteins and lipids in live cell membranes of eukaryotic cells, it has been found that membranes contain a mosaic of fluid compartments....

journal_title：Biopolymers

authors： Ritchie K,Spector J

更新日期：2007-10-05 00:00:00

abstract：:Despite detailed knowledge of the overall structural changes and stoichiometries of surfactant binding, little is known about which protein regions constitute the preferred sites of attack for initial unfolding. Here we have exposed three proteins to limited proteolysis at anionic (SDS) and cationic (DTAC) surfactant ...

journal_title：Biopolymers

authors： Hansen JH,Petersen SV,Andersen KK,Enghild JJ,Damhus T,Otzen D

更新日期：2009-03-01 00:00:00

abstract：:Glycopeptide analogues of CSF114(Glc), modified at N-terminus with new ferrocenyl carboxylic acid and a new ferrocenyl-thiphosphino amino acid, were used to implement a new electrochemical biosensor for autoantibody detection in multiple sclerosis. The ferrocenyl moiety of these "electrochemical probes" did not affect...

journal_title：Biopolymers

authors： Real-Fernández F,Colson A,Bayardon J,Nuti F,Peroni E,Meunier-Prest R,Lolli F,Chelli M,Darcel C,Jugé S,Papini AM

更新日期：2008-01-01 00:00:00

abstract：:The solution conformations of a hybrid sequence peptide related to the bee venom peptide apamin have been determined using two-dimensional 1H-nmr. Apamin is an 18 amino acid peptide containing a C-terminal helix that is stabilized by two disulfide bonds. The deletion of one residue (K4) of the N-terminal "scaffold" re...

journal_title：Biopolymers

authors： Volkman BF,Wemmer DE

更新日期：1997-04-05 00:00:00

abstract：:The self-cleaving hepatitis delta virus (HDV) ribozyme is essential for the replication of HDV, a liver disease causing pathogen in humans. The catalytically critical nucleotide C75 of the ribozyme is buttressed by a trefoil turn pivoting around an extruded G76. In all available crystal structures, the conformation of...

journal_title：Biopolymers

authors： Sefcikova J,Krasovska MV,Spacková N,Sponer J,Walter NG

更新日期：2007-04-05 00:00:00

abstract：:Understanding the high temperature behavior of collagen and collagenous tissue is important for surgical procedures and biomaterials processing for the food, pharmaceutical, and cosmetics industries. One primary event for proteins is thermal denaturation that involves unfolding the polypeptide chains while maintaining...

journal_title：Biopolymers

authors： Fields M,Spencer N,Dudhia J,McMillan PF

更新日期：2017-06-01 00:00:00

abstract：:Hyaluronic acid (HA) is an important biomacromolecule, which fulfils a number of vital physiological functions (especially in the joint synovial fluid) and also has consumer and pharmaceutical applications. HA solution properties have already been quite thoroughly characterized in response to steady shear flows but ar...

journal_title：Biopolymers

authors： Haward SJ

更新日期：2014-03-01 00:00:00