The interaction of ellipticine derivatives with nucleic acids studied by optical and 1H-nmr spectroscopy: effect of size of the heterocyclic ring system.


:The DNA interaction of derivatives of ellipticine with heterocyclic ring systems with three, four, or five rings and a dimethylaminoethyl side chain was studied. Optical spectroscopy of drug complexes with calf thymus DNA, poly[(dA-dT).(dA-dT)], or poly[(dG-dC).(dG-dC)] showed a 10 nm bathochromic shift of the light absorption bands of the pentacyclic and tetracyclic compounds upon binding to the nucleic acids, which indicates binding by intercalation. For the tricyclic compound a smaller shift of 1-3 nm was observed upon binding to the nucleic acids. Flow linear dichroism studies show that the geometry of all complexes is consistent with intercalation of the ring system, except for the DNA and poly[(dG-dC).(dG-dC)] complexes of the tricyclic compound, where the average angle between the drug molecular plane and the DNA helix axis was found to be 65 degrees. One-dimensional 1H-nmr spectroscopy was used to study complexes between d(CGCGATCGCG)2 and the tricyclic and pentacyclic compounds. The results on the pentacyclic compound show nonselective broadening due to intermediate chemical exchange of most oligonucleotide resonances upon drug binding. The imino proton resonances are in slow chemical exchange, and new resonances with upfield shifts approaching 1 ppm appear upon drug binding, which supports intercalative binding of the pentacyclic compound. The results on the tricyclic compound show more rapid binding kinetics and very selective broadening of resonances. The data suggest that the tricyclic compound is in an equilibrium between intercalation and minor groove binding, with a preference to bind close to the AT base pairs with the side chain residing in the minor groove.






Behravan G,Leijon M,Sehlstedt U,Nordén B,Vallberg H,Bergman J,Gräslund A




Has Abstract


1994-05-01 00:00:00












  • The question of long-range allosteric transitions in DNA.

    abstract::The question of long-range allosteric transitions of DNA secondary structure and their possible involvement in transcriptional activation is discussed in the light of new results. A variety of recent evidence strongly supports a fluctuating long-range description of DNA secondary structure. Balanced equilibria between...


    pub_type: 杂志文章,评审


    authors: Schurr JM,Delrow JJ,Fujimoto BS,Benight AS

    更新日期:1997-01-01 00:00:00

  • Conformational preferences of peptide-peptoid hybrid oligomers.

    abstract::Peptomers are oligomeric molecules composed of both α-amino acids and N-substituted glycine monomers, thus creating a hybrid of peptide and peptoid units. Peptomers have been used in several applications such as antimicrobials, protease inhibitors, and antibody mimics. Despite the considerable promise of peptomers as ...


    pub_type: 杂志文章


    authors: Butterfoss GL,Drew K,Renfrew PD,Kirshenbaum K,Bonneau R

    更新日期:2014-09-01 00:00:00

  • Molecular dynamics studies of the antimicrobial peptides piscidin 1 and its mutants with a DOPC lipid bilayer.

    abstract::Piscidin 1 (Pis-1) has a high broad-spectrum activity against bacteria, fungi, and viruses but it also has a moderate hemolytic activities. To improve the antibacterial activity and to reduce toxicity, mutants Pis-1AA (G8A/G13A double mutant) and Pis-1PG (G8P mutant) have been designed based on the crystal structure o...


    pub_type: 杂志文章


    authors: Yuan T,Zhang X,Hu Z,Wang F,Lei M

    更新日期:2012-12-01 00:00:00

  • Identification and characterization of multifunctional cationic and amphipathic peptides from soybean proteins.

    abstract::In this study, we identified and chemically synthesized three cationic and amphipathic peptides (Glycinin-17, BCAS-16, and BCBS-11) from soybean proteins. These peptides had high isoelectric points, high positive net charges, and included multiple hydrophobic amino acids. Subsequently, we identified multiple functions...


    pub_type: 杂志文章


    authors: Taniguchi M,Saito K,Nomoto T,Namae T,Ochiai A,Saitoh E,Tanaka T

    更新日期:2017-07-01 00:00:00

  • Fourier transform infared spectroscopy investigation of protein conformation in spray-dried protein/trehalose powders.

    abstract::Spray drying is a way to generate protein solids (powders), which is also true for lyophilization. Sugars are used to protect proteins from conformational changes and chemical degradations arising from drying processes and storage conditions such as the humidity. The influence of trehalose and humidity on the conforma...


    pub_type: 杂志文章


    authors: French DL,Arakawa T,Li T

    更新日期:2004-03-01 00:00:00

  • CD and fluorescence studies of the human granulocyte-macrophage colony-stimulating factor and related peptide conformations in aqueous solution.

    abstract::The absorption, CD, and fluorescence emission spectra, and the fluorescence emission and depolarization lifetimes of the human granulocyte-macrophage colony-stimulating factor (hGM-CSF) and related peptides previously tested for their immunological activity, were measured in water at various pHs and temperatures to ob...


    pub_type: 杂志文章


    authors: Casté L,Zandomeneghi M,Houben JL,Rovero P,Pegoraro S,Revoltella PR,Brochon JC

    更新日期:1995-07-01 00:00:00

  • Stabilization of a novel beta-turn-like motif by nonconventional intramolecular hydrogen-bonding interactions in a model peptide incorporating beta-alanine.

    abstract::The chemical synthesis and x-ray crystal structure analysis of a model peptide incorporating a conformationally adaptable unsubstituted beta-Ala residue: Boc-beta-Ala-Acc6-OCH3 (C16H28N2O5, molecular weight = 328.41; 1) has been described. The peptide crystallized in the space group P2(1)2(1)2(1) a = 8.537 (3), b = 8....


    pub_type: 杂志文章


    authors: Thakur AK,Kishore R

    更新日期:2000-05-01 00:00:00

  • An evaluation of Poisson-Boltzmann electrostatic free energy calculations through comparison with experimental mutagenesis data.

    abstract::For systems involving highly and oppositely charged proteins, electrostatic forces dominate association and contribute to biomolecular complex stability. Using experimental or theoretical alanine-scanning mutagenesis, it is possible to elucidate the contribution of individual ionizable amino acids to protein associati...


    pub_type: 杂志文章


    authors: Gorham RD Jr,Kieslich CA,Nichols A,Sausman NU,Foronda M,Morikis D

    更新日期:2011-11-01 00:00:00

  • Fast membrane association is a crucial factor in the peptide pep-1 translocation mechanism: a kinetic study followed by surface plasmon resonance.

    abstract::The use of peptide carriers, termed "cell-penetrating peptides (CPPs)" has attracted much attention due to their potential for cellular delivery of hydrophilic molecules with pharmacological interest, overcoming the membrane barrier. These peptides are able to deliver attached cargos in a nontoxic manner, with the upt...


    pub_type: 杂志文章


    authors: Henriques ST,Castanho MA,Pattenden LK,Aguilar MI

    更新日期:2010-01-01 00:00:00

  • Bone marrow immunoregulatory peptides (myelopeptides): isolation, structure, and functional activity.

    abstract::Myelopeptides (MPs) are bioregulatory mediators of bone marrow origin. Several individual MPs have been isolated from the supernatant of porcine bone marrow cell culture by successive solid phase extraction and reversed-phase high performance liquid chromatography. Two of them, MP-1 (Phe-Leu-Gly-Phe-Pro-Thr) and MP-2 ...


    pub_type: 杂志文章,评审


    authors: Petrov RV,Mikhailova AA,Fonina LA

    更新日期:1997-01-01 00:00:00

  • Revisiting "reverse hydrophobic effect": applicable only to coil mutations at the surface.

    abstract::In a seminal paper, Pakula and Sauer (Nature, 1990, 344, 363-364) demonstrated that the increase in side-chain hydrophobicity has a reverse relationship with protein stability. We have addressed this problem with several examples of mutants that span at different locations in protein structure based on secondary struc...


    pub_type: 杂志文章


    authors: Gromiha MM

    更新日期:2009-07-01 00:00:00

  • A model for type II collagen fibrils: distinctive D-band patterns in native and reconstituted fibrils compared with sequence data for helix and telopeptide domains.

    abstract::The periodical D-band pattern is generally considered a unique ultrastructural feature shared by all fibril-forming collagens, which correlates with the intrafibril, paracrystalline array of tropocollagen monomers. Distinct band patterns have been reported, however, for collagen stained long-spacing (SLS) crystallites...


    pub_type: 杂志文章


    authors: Ortolani F,Giordano M,Marchini M

    更新日期:2000-11-01 00:00:00

  • Crystal structure of cyclic (APGVGV)2, an analog of elastin, and a suggested mechanism for elongation/contraction of the molecule.

    abstract::Tropoelastin is a complex polymeric protein composed primarily of repeating segments of Val-Pro-Gly-Gly, Val-Pro-Gly-Val-Gly, and Ala-Pro-Gly-Val-Gly-Val that occurs in connective tissue and arteries. It has rubber-like extensible properties. A synthetic cyclic dodecapeptide, with a double repeat of the hexapeptide se...


    pub_type: 杂志文章


    authors: Karle IL,Urry DW

    更新日期:2005-03-01 00:00:00

  • The spectroscopic properties of the lipodepsipeptide, syringomycin E.

    abstract::The spectroscopic properties of syringomycin E, an antibiotic lipodepsinonapeptide associated with pathological states in plants, have been investigated by uv absorbance and CD spectroscopies, and by the synthesis of relevant model compounds. Initial studies [E. Vaillo, A. Ballio, P. L. Luisi, and R. M. Thomas (1990) ...


    pub_type: 杂志文章


    authors: Vaillo E,Ballio A,Luisi PL,Thomas RM

    更新日期:1992-10-01 00:00:00

  • Synthesis and characterization of the sweet protein brazzein.

    abstract::The sweet protein brazzein isolated from the fruit of the African plant, Pentadiplandra brazzeana Baillon is 2000-500 times sweeter than sucrose and consists of 54 amino acid residues with four intramolecular disulfide bonds. Brazzein was prepared by the fluoren-9-yl-methoxycarbonyl solid-phase method, and was identic...


    pub_type: 杂志文章


    authors: Izawa H,Ota M,Kohmura M,Ariyoshi Y

    更新日期:1996-07-01 00:00:00

  • Sr(2+) induces unusually stable d(GGGTGGGTGGGTGGG) quadruplex dimers.

    abstract::Guanine-rich sequences are able to form quadruplexes consisting of G-quartet structural units. Quadruplexes play an important role in the regulation of gene expression and have therapeutic and biotechnological potential. The HIV-1 integrase inhibitor, (GGGT)4 , and its variants demonstrate unusually high thermal stabi...


    pub_type: 杂志文章


    authors: Lomidze L,Kelley S,Gogichaishvili S,Metreveli N,Musier-Forsyth K,Kankia B

    更新日期:2016-11-01 00:00:00

  • The hydration of nucleic acid duplexes as assessed by a combination of volumetric and structural techniques.

    abstract::Using high precision densimetric and ultrasonic measurements, we have determined, at 25 degrees C, the apparent molar volumes PhiV and the apparent molar compressibilities PhiK(S) of four nucleic acid duplexes-namely, the DNA duplex, poly(dIdC)poly(dIdC); the RNA duplex, poly(rA)poly(rU); and the two DNA/RNA hybrid du...


    pub_type: 杂志文章


    authors: Chalikian TV,Völker J,Srinivasan AR,Olson WK,Breslauer KJ

    更新日期:1999-10-15 00:00:00

  • Estimation of strength in different extra Watson-Crick hydrogen bonds in DNA double helices through quantum chemical studies.

    abstract::It was shown earlier, from database analysis, model building studies, and molecular dynamics simulations that formation of cross-strand bifurcated or Extra Watson-Crick hydrogen (EWC) bonds between successive base pairs may lead to extra rigidity to DNA double helices of certain sequences. The strengths of these hydro...


    pub_type: 杂志文章


    authors: Bandyopadhyay D,Bhattacharyya D

    更新日期:2006-10-15 00:00:00

  • Role of water molecules in the crystal structure of Gly-L-Ala-L-Phe: a possible sequence preference for nucleation of alpha-helix?

    abstract::The synthetic peptide Gly-L-Ala-L-Phe (C14H19N3O4.2H2O; GAF) crystallizes in the monoclinic space group P2I1), with a = 5.879(1), b = 7.966(1), c = 17.754(2) A, beta = 95.14(2) degrees, Dx = 1.321 g cm-3, and Z = 2. The crystal structure was solved by direct methods using the program SHELXS-86 and refined to an R valu...


    pub_type: 杂志文章


    authors: Ramasubbu N,Parthasarathy R

    更新日期:1989-07-01 00:00:00

  • An improved model for analyzing the small angle x-ray scattering of starch granules.

    abstract::The structure of starch was studied using small-angle x-ray scattering (SAXS). The scattering data was modeled by considering a finite stack of alternating lamellae that are allowed to fluctuate both along the layer repeat direction and along the transverse layer direction. Analysis in this way of the SAXS data from s...


    pub_type: 杂志文章


    authors: Daniels DR,Donald AM

    更新日期:2003-06-01 00:00:00

  • De novo design of α,β-didehydrophenylalanine containing peptides: from models to applications.

    abstract::The de novo design of peptides and proteins has emerged as an approach for investigating protein structure and function. The success relies heavily on the ability to design relatively short peptides that can adopt stable secondary structures. To this end, substitution with α,β-dehydroamino acids, especially α,β-didehy...


    pub_type: 杂志文章,评审


    authors: Gupta M,Chauhan VS

    更新日期:2011-03-01 00:00:00

  • Expression and biophysical analysis of a triple-transmembrane domain-containing fragment from a yeast G protein-coupled receptor.

    abstract::Structural characterization of G protein-coupled receptors (GPCRs) is hindered by the inherent hydrophobicity, flexibility, and large size of these signaling proteins. Insights into conformational preferences and the three-dimensional (3D) structure of domains of these receptors can be obtained using polypeptide fragm...


    pub_type: 杂志文章


    authors: Caroccia KE,Estephan R,Cohen LS,Arshava B,Hauser M,Zerbe O,Becker JM,Naider F

    更新日期:2011-01-01 00:00:00

  • Nondestructive FTIR monitoring of leaf senescence and elicitin-induced changes in plant leaves.

    abstract::The applicability of the FTIR attenuated total reflectance technique for in situ monitoring of plant physiological processes such as leaf senescence and aging has been examined. Difference spectra obtained by subtracting the spectrum of the young plant leaf from that of the older one revealed positive bands at 1650-15...


    pub_type: 杂志文章


    authors: Ivanova DG,Singh BR

    更新日期:2003-01-01 00:00:00

  • Conformational and structural analysis of the equilibrium between single- and double-strand beta-helix of a D,L-alternating oligonorleucine.

    abstract::Alternating sequences of D and L residues in peptides are directly related to the formation of several kinds of regular helical conformations usually called beta-helices. The major feature of these structures is that they can be associated with the transmembrane ion-conducting channel activity in some natural antibact...


    pub_type: 杂志文章


    authors: Navarro E,Fenude E,Celda B

    更新日期:2004-02-05 00:00:00

  • Peptide methionine sulfoxide reductase: biochemistry and physiological role.

    abstract::The oxidation of methionine to methionine sulfoxide both in vivo and in vitro can lead to the loss of biological activity in a variety of proteins. This loss of activity can be reversed by an enzyme called methionine sulfoxide reductase. The gene for this enzyme has been cloned and sequenced from a variety of prokaryo...


    pub_type: 杂志文章,评审


    authors: Brot N,Weissbach H

    更新日期:2000-01-01 00:00:00

  • CD and (13)C(alpha)-NMR studies of folding equilibria in a two-stranded coiled coil formed by residues 190-254 of alpha-tropomyosin.

    abstract::Synthesis and CD and (13)C(alpha)-NMR studies in a near-neutral saline buffer are reported for a 65-residue peptide ((190)Tm(254)) comprising residues 190-254 of the alpha-tropomyosin chain. CD on a version disulfide cross-linked via the N-terminal cysteine side chains indicates that this dimer is highly helical and m...


    pub_type: 杂志文章


    authors: Emerson Holtzer M,Mints L,Hogue Angeletti R,d'Avignon DA,Holtzer A

    更新日期:2001-10-05 00:00:00

  • Molecular mass and chain conformation of carboxymethylated derivatives of beta-glucan from sclerotia of Pleurotus tuber-regium.

    abstract::Seven water-insoluble (1 --> 3)-beta-D-glucan fractions TM8-1 to TM8-7 with weight-average molecular mass M(w) ranged from 2.22 to 77.4 x 10(4) obtained from the sclerotia of Pleurotus tuber-regium were carboxymethylated to produce the water-soluble fractions CTM8-1 to CTM8-7 with M(w) ranged from 3.87 to 87.8 x 10(4)...


    pub_type: 杂志文章


    authors: Zhang M,Zhang L,Cheung PC

    更新日期:2003-02-01 00:00:00

  • The role of DNA-protein salt bridges in molecular recognition: a model study.

    abstract::A theoretical study is presented of the influence of salt bridges between cationic side chains and DNA phosphates on DNA conformation and flexibility. The DNA sequence studied is that of the catabolite activator protein binding oligomer from the crystallized complex. The effect of salt bridges is modeled by neutraliza...


    pub_type: 杂志文章


    authors: Gurlie R,Duong TH,Zakrzewska K

    更新日期:1999-04-01 00:00:00

  • Solid-state conformations of aminosuccinyl peptides: crystal structure of tert-butyloxycarbonyl-L-leucyl-L-aminosuccinyl-L-phenylalaninami de.

    abstract::The protected tripeptide tert-butyloxycarbonyl-L-leucyl-L-aminosuccinyl-L-phenylalaninamide crystallizes in the orthorhombic space group P2(1)2(1)2(1), with a = 6.214(3), b = 12.832(3), c = 33.094(4) A, Z = 4. The structure was solved by direct methods using MULTAN 80 and refined to an R value of 0.055 for 1458 reflec...


    pub_type: 杂志文章


    authors: Capasso S,Mazzarella L,Sica F,Zagari A

    更新日期:1989-01-01 00:00:00

  • Multinuclear NMR and FTIR studies of new polyoxaalkyl esters of lasalocid and their complexes with lithium and sodium cations.

    abstract::Three new polyoxaalkyl esters of lasalocid are synthesized. Their ability to form complexes with Li(+) and Na(+) cations is studied using multinuclear NMR methods, FTIR spectroscopy in the middle and far IR regions, and mass spectrometry. It is found that lasalocid esters form only 1:1 complexes with the metal cations...


    pub_type: 杂志文章


    authors: Pankiewicz R,Schroeder G,Gierczyk B,Brzezinski B,Bartl F

    更新日期:2002-10-15 00:00:00